Proper orientation of the mitotic spindle is critical for successful cell division in budding yeast. To investigate the mechanism of spindle orientation, we used a green fluorescent protein ...(GFP)-tubulin fusion protein to observe microtubules in living yeast cells. GFP-tubulin is incorporated into microtubules, allowing visualization of both cytoplasmic and spindle microtubules, and does not interfere with normal microtubule function. Microtubules in yeast cells exhibit dynamic instability, although they grow and shrink more slowly than microtubules in animal cells. The dynamic properties of yeast microtubules are modulated during the cell cycle. The behavior of cytoplasmic microtubules revealed distinct interactions with the cell cortex that result in associated spindle movement and orientation. Dynein-mutant cells had defects in these cortical interactions, resulting in misoriented spindles. In addition, microtubule dynamics were altered in the absence of dynein. These results indicate that microtubules and dynein interact to produce dynamic cortical interactions, and that these interactions result in the force driving spindle orientation.
Lymphocytes home to lymph nodes, using L-selectin to bind specific ligands on high endothelial venules (HEV). In vitro studies implicate GlcNAc-6-sulfate as an essential posttranslational ...modification for ligand activity. Here, we show that genetic deletion of HEC-GlcNAc6ST, a sulfotransferase that is highly restricted to HEV, results in the loss of the binding of recombinant L-selectin to the luminal aspect of HEV, elimination of lymphocyte binding in vitro, and markedly reduced in vivo homing. Reactivity with MECA 79, an adhesion-blocking mAb that stains HEV in lymph nodes and vessels in chronic inflammatory sites, is also lost from the luminal aspects of HEV. These results establish a critical role for HEC-GlcNAc6ST in lymphocyte trafficking and suggest it as an important therapeutic target.
The standard model of particle physics
describes the known fundamental particles and forces that make up our Universe, with the exception of gravity. One of the central features of the standard model ...is a field that permeates all of space and interacts with fundamental particles
. The quantum excitation of this field, known as the Higgs field, manifests itself as the Higgs boson, the only fundamental particle with no spin. In 2012, a particle with properties consistent with the Higgs boson of the standard model was observed by the ATLAS and CMS experiments at the Large Hadron Collider at CERN
. Since then, more than 30 times as many Higgs bosons have been recorded by the ATLAS experiment, enabling much more precise measurements and new tests of the theory. Here, on the basis of this larger dataset, we combine an unprecedented number of production and decay processes of the Higgs boson to scrutinize its interactions with elementary particles. Interactions with gluons, photons, and W and Z bosons-the carriers of the strong, electromagnetic and weak forces-are studied in detail. Interactions with three third-generation matter particles (bottom (b) and top (t) quarks, and tau leptons (τ)) are well measured and indications of interactions with a second-generation particle (muons, μ) are emerging. These tests reveal that the Higgs boson discovered ten years ago is remarkably consistent with the predictions of the theory and provide stringent constraints on many models of new phenomena beyond the standard model.
This article documents the muon reconstruction and identification efficiency obtained by the ATLAS experiment for 139
fb
-
1
of
pp
collision data at
s
=
13
TeV collected between 2015 and 2018 during ...Run 2 of the LHC. The increased instantaneous luminosity delivered by the LHC over this period required a reoptimisation of the criteria for the identification of prompt muons. Improved and newly developed algorithms were deployed to preserve high muon identification efficiency with a low misidentification rate and good momentum resolution. The availability of large samples of
Z
→
μ
μ
and
J
/
ψ
→
μ
μ
decays, and the minimisation of systematic uncertainties, allows the efficiencies of criteria for muon identification, primary vertex association, and isolation to be measured with an accuracy at the per-mille level in the bulk of the phase space, and up to the percent level in complex kinematic configurations. Excellent performance is achieved over a range of transverse momenta from 3 GeV to several hundred GeV, and across the full muon detector acceptance of
|
η
|
<
2.7
.
Jet substructure observables have significantly extended the search program for physics beyond the standard model at the Large Hadron Collider. The state-of-the-art tools have been motivated by ...theoretical calculations, but there has never been a direct comparison between data and calculations of jet substructure observables that are accurate beyond leading-logarithm approximation. Such observables are significant not only for probing the collinear regime of QCD that is largely unexplored at a hadron collider, but also for improving the understanding of jet substructure properties that are used in many studies at the Large Hadron Collider. This Letter documents a measurement of the first jet substructure quantity at a hadron collider to be calculated at next-to-next-to-leading-logarithm accuracy. The normalized, differential cross section is measured as a function of log10ρ2, where ρ is the ratio of the soft-drop mass to the ungroomed jet transverse momentum. This quantity is measured in dijet events from 32.9 fb−1 of s=13 TeV proton-proton collisions recorded by the ATLAS detector. The data are unfolded to correct for detector effects and compared to precise QCD calculations and leading-logarithm particle-level Monte Carlo simulations.
A search for the electroweak production of charginos and sleptons decaying into final states with two electrons or muons is presented. The analysis is based on 139 fb−1 of proton–proton collisions ...recorded by the ATLAS detector at the Large Hadron Collider at s√=13 TeV. Three R-parity-conserving scenarios where the lightest neutralino is the lightest supersymmetric particle are considered: the production of chargino pairs with decays via either W bosons or sleptons, and the direct production of slepton pairs. The analysis is optimised for the first of these scenarios, but the results are also interpreted in the others. No significant deviations from the Standard Model expectations are observed and limits at 95% confidence level are set on the masses of relevant supersymmetric particles in each of the scenarios. For a massless lightest neutralino, masses up to 420 GeV are excluded for the production of the lightest-chargino pairs assuming W-boson-mediated decays and up to 1 TeV for slepton-mediated decays, whereas for slepton-pair production masses up to 700 GeV are excluded assuming three generations of mass-degenerate sleptons.
The algorithms used by the ATLAS Collaboration during Run 2 of the Large Hadron Collider to identify jets containing
b
-hadrons are presented. The performance of the algorithms is evaluated in the ...simulation and the efficiency with which these algorithms identify jets containing
b
-hadrons is measured in collision data. The measurement uses a likelihood-based method in a sample highly enriched in
t
t
¯
events. The topology of the
t
→
W
b
decays is exploited to simultaneously measure both the jet flavour composition of the sample and the efficiency in a transverse momentum range from 20 to 600 GeV. The efficiency measurement is subsequently compared with that predicted by the simulation. The data used in this measurement, corresponding to a total integrated luminosity of 80.5
fb
-
1
, were collected in proton–proton collisions during the years 2015–2017 at a centre-of-mass energy
s
=
13 TeV. By simultaneously extracting both the efficiency and jet flavour composition, this measurement significantly improves the precision compared to previous results, with uncertainties ranging from 1 to 8% depending on the jet transverse momentum.
Jet energy scale measurements and their systematic uncertainties are reported for jets measured with the ATLAS detector using proton-proton collision data with a center-of-mass energy of s=13 TeV, ...corresponding to an integrated luminosity of 3.2 fb−1 collected during 2015 at the LHC. Jets are reconstructed from energy deposits forming topological clusters of calorimeter cells, using the anti-kt algorithm with radius parameter R=0.4. Jets are calibrated with a series of simulation-based corrections and in situ techniques. In situ techniques exploit the transverse momentum balance between a jet and a reference object such as a photon, Z boson, or multijet system for jets with 20<pT<2000 GeV and pseudorapidities of |η|<4.5, using both data and simulation. An uncertainty in the jet energy scale of less than 1% is found in the central calorimeter region (|η|<1.2) for jets with 100<pT<500 GeV. An uncertainty of about 4.5% is found for low-pT jets with pT=20 GeV in the central region, dominated by uncertainties in the corrections for multiple proton-proton interactions. The calibration of forward jets (|η|>0.8) is derived from dijet pT balance measurements. For jets of pT=80 GeV, the additional uncertainty for the forward jet calibration reaches its largest value of about 2% in the range |η|>3.5 and in a narrow slice of 2.2<|η|<2.4.
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