The posterior superior temporal sulcus (STS) region plays an important role in the perception of social acts, although its full role has not been completely clarified. This functional magnetic ...resonance imaging experiment examined activity in the STS region as participants viewed actions that were congruent or incongruent with intentions established by a previous emotional context. Participants viewed an actress express either a positive or a negative emotion toward one of two objects and then subsequently pick up one of them. If the object that was picked up had received positive regard, or if the object that was not picked up had received negative regard, the action was congruent; otherwise, the action was incongruent. Activity in the right posterior STS region was sensitive to the congruency between the action and the actress's emotional expression (i.e., STS activity was greater on incongruent than on congruent trials). These findings suggest that the posterior STS represents not only biological motion, but also how another person's motion is related to his or her intentions.
Sustaining food security under climate conditions expected for the 21st century will require that existing crop production systems simultaneously increase both productivity and resiliency to warmer ...and more variable climate conditions. In this study, we analyzed observational rainfed maize (Zea mays L.) yield data from major maize production areas of the US Corn Belt. These data included detailed information on crop management and genetics not typically available in observational studies, allowing us to better understand maize yield response to climate under variable management. Spatial variability in management variables across the study domain is coincident with spatial climate gradients. Regularized global and geographically weighted regression models were used to explore maize yield response to climate, management, genetics, and their interactions, while accounting for collinearity among them associated with corresponding scales of spatial variability. In contrast with recent analyses suggesting increased susceptibility to drought stress under higher plant populations in maize production, our analyses indicated that under moisture limitation, higher yields were achieved when high planting rates were coupled with delayed planting date. Maize genetic families that performed best with adequate moisture saw greater yield penalties under moisture limited conditions, while positive response to increased radiation was consistent among family lines. The magnitude of yield response to climate, management, and their interactions was also variable across the study domain, suggesting that information on crop management in spatial yield data can be used to better tailor local management practices to changes in yield potential resulting from agronomic advancements and changing local climate.
To assess the accuracy of point-of-care ultrasound (PoCUS) in the hands of two trained and blinded emergency physicians (EPs) in detecting very small amounts of free intraperitoneal air injected ...intra-abdominally, using a fresh human cadaver model.
Fifteen cadavers were injected on 3 occasions with predefined quantities of free intraperitoneal air ranging from 0-10 mL. Seven cadavers were injected in the mid-epigastrium (ME), while 8 were injected in the left lower quadrant (LLQ). Each cadaver was scanned after each of the 3 injections by 2 trained and blinded EPs, resulting in 45 scans per sonographer. Scans were performed using previously validated and standardized techniques. All scans were recorded, time-stamped and labeled. For each scan the sonographers indicated "yes" or "no" to whether pneumoperitoneum was detected. A chi square analysis was performed to determine the sensitivity and specificity of PoCUS utilized by each sonographer of pneumoperitoneum based on the location and volume of air injected.
Free air (0.25-10 mL) injected into the ME was successfully diagnosed in 36/42 instances (86% sensitivity), but only detected in 10/36 instances when injected into the LLQ (28% sensitivity). Both EPs detected all air injections of ≥2 mL into the ME.
Detection of free air originating from the midepigastric region may become a future PoCUS indication for adequately trained EPs.
Abstract
In August 2022, Death Valley, the driest place in North America, experienced record flooding from summertime rainfall associated with the North American monsoon (NAM). Given the ...socioeconomic cost of these type of events, there is a dire need to understand their drivers and future statistics. Existing theory predicts that increases in the intensity of precipitation is a robust response to anthropogenic warming. Paleoclimatic evidence suggests that northeast Pacific (NEP) sea surface temperature (SST) variability could further intensify summertime NAM rainfall over the desert southwest. Drawing on this paleoclimatic evidence, we use historical observations and reanalyzes to test the hypothesis that warm SSTs on the southern California margin are linked to more frequent extreme precipitation events in the NAM domain. We find that summers with above-average coastal SSTs are more favorable to moist convection in the northern edge of the NAM domain (southern California, Arizona, New Mexico, and the southern Great Basin). This is because warmer SSTs drive circulation changes that increase moisture flux into the desert southwest, driving more frequent precipitation extremes and increases in seasonal rainfall totals. These results, which are robust across observational products, establish a linkage between marine and terrestrial extremes, since summers with anomalously warm SSTs on the California margin have been linked to seasonal or multi-year NEP marine heatwaves. However, current generation earth system models (ESMs) struggle to reproduce the observed relationship between coastal SSTs and NAM precipitation. Across models, there is a strong negative relationship between the magnitude of an ESM’s warm SST bias on the California margin and its skill at reproducing the correlation with desert southwest rainfall. Given persistent NEP SST biases in ESMs, our results suggest that efforts to improve representation of climatological SSTs are crucial for accurately predicting future changes in hydroclimate extremes in the desert southwest.
Molecular analysis of proaerolysin selected glycosylphosphatidylinositol anchor (GPI-a) deficient isolates in the TK6 cell line was performed. Initial studies found that the expected X-linked PIGA ...mutations were rare among the spontaneous isolates but did increase modestly after ethyl methane sulfate (EMS) treatment (but to only 50% of isolates). To determine the molecular bases of the remaining GPI-a deficient isolates, real-time analysis for all the 25 autosomal GPI-a pathway genes was performed on the isolates without PIGA mutations, determining that PIGL mRNA was absent for many. Further analysis determined these isolates had several different homozygous deletions of the 5' region of PIGL (17p12-p22) extending 5' (telomeric) through NCOR1 and some into the TTC19 gene (total deletion >250,000 bp). It was determined that the TK6 parent had a hemizygous deletion in 17p12-p22 (275,712 bp) extending from PIGL intron 2 into TTC19 intron 7. Second hit deletions in the other allele in the GPI-a deficient isolates led to the detected homozygous deletions. Several of the deletion breakpoints including the original first hit deletion were sequenced. As strong support for TK6 having a deletion, a number of the isolates without PIGA mutations nor homozygous PIGL deletions had point mutations in the PIGL gene. These studies show that the GPI-a mutation studies using TK6 cell line could be a valuable assay detecting point and deletion mutations in two genes simultaneously.
•Level of depression decreased over time during pregnancy and postpartum.•Language, partner status, and prior depression significantly impacted prenatal trajectories.•Depression at the time of ...delivery was the best predictor of postpartum depression trajectory.
Despite high rates of perinatal depression among women from diverse backgrounds, the understanding of the trajectory of depressive symptoms is limited. The aim of this study was to investigate the trajectories of depressive symptoms from pregnancy to postpartum among an international sample of pregnant women.
Hispanic/Latina (79.2%), Spanish-speaking (81%) pregnant women (N = 1796; Mean age = 28.32, SD = 5.51) representing 78 unique countries/territories participated in this study. A sequential-process latent growth-curve model was estimated to examine general trajectories of depression as well as risk and protective factors that may impact depression levels throughout both the prenatal and postpartum periods.
Overall, depression levels decreased significantly across the entire perinatal period, but this decrease slowed over time within both the prenatal and postpartum periods. Spanish-speaking women, those who were partnered, and those with no history of depression reported lower levels of depression during early pregnancy, but this buffer effect reduced over time. Depression levels at delivery best predicted postpartum depression trajectories (i.e., women with higher levels of depression at delivery were at greater risk for depression postpartum).
Given the emphasis on language and not country or culture of origin this study was limited in its ability to examine the impact of specific cultural norms and expectations on perinatal depression.
Given these findings, it is imperative that providers pay attention to, and assess for, depressive symptoms and identified buffers for depression, especially when working with women from diverse communities.
ABSTRACT
Microvesicles (MVs) are involved in cell‐cell interactions, including disease pathogenesis. Nondestructive Fourier‐transform infrared (FTIR) spectra from MVs were assessed as a technique to ...provide new biochemical insights into a LPS‐induced monocyte model of septic shock. FTIR spectroscopy provided a quick method to investigate relative differences in biomolecular content of different MV populations that was complementary to traditional semiquantitative omics approaches, with which it is difficult to provide information on relative changes between classes (proteins, lipids, nucleic acids, carbohydrates) or protein conformations. Time‐dependent changes were detected in biomolecular contents of MVs and in the monocytes from which they were released. Differences in phosphatidylcholine and phosphatidylserine contents were observed in MVs released under stimulation, and higher relative concentrations of RNA and a‐helical structured proteins were present in stimulated MVs compared with MVs from resting cells. FTIR spectra of stimulated monocytes displayed changes that were consistent with those observed in the corresponding MVs they released. LPS‐stimulated monocytes had reduced concentrations of nucleic acids, a‐helical structured proteins, and phosphatidylcholine compared with resting monocytes but had an increase in total lipids. FTIR spectra of MV biomolecular content will be important in shedding new light on the mechanisms of MVs and the different roles they play in physiology and disease pathogenesis.—Lee, J., Wen, B., Carter, E. A., Combes, V., Grau, G. E. R., Lay, P. A. Infrared spectroscopic characterization of monocytic microvesicles (microparticles) released upon lipopolysaccharide stimulation. FASEB J. 31, 2817–2827 (2017). www.fasebj.org
The trimeric HIV-1 Envelope protein (Env) mediates viral-host cell fusion via a network of conformational transitions, with allosteric elements in each protomer orchestrating host receptor-induced ...exposure of the co-receptor binding site and fusion elements. To understand the molecular details of this allostery, here, we introduce Env mutations aimed to prevent CD4-induced rearrangements in the HIV-1 BG505 Env trimer. Binding analysis and single-molecule Förster Resonance Energy Transfer confirm that these mutations prevent CD4-induced transitions of the HIV-1 Env. Structural analysis by single-particle cryo-electron microscopy performed on the BG505 SOSIP mutant Env proteins shows rearrangements in the gp120 topological layer contacts with gp41. Displacement of a conserved tryptophan (W571) from its typical pocket in these Env mutants renders the Env insensitive to CD4 binding. These results reveal the critical function of W571 as a conformational switch in Env allostery and receptor-mediated viral entry and provide insights on Env conformation that are relevant for vaccine design.
Confocal Raman spectroscopy is introduced as a noninvasive in vivo optical method to measure molecular concentration profiles in the skin. It is shown how it can be applied to determine the water ...concentration in the stratum corneum as a function of distance to the skin surface, with a depth resolution of 5 μm. The resulting in vivo concentration profiles are in qualitative and quantitative agreement with published data, obtained by in vitro X-ray microanalysis of skin samples. Semi-quantitative concentration profiles were determined for the major constituents of natural moisturizing factor (serine, glycine, pyrrolidone-5-carboxylic acid, arginine, ornithine, citrulline, alanine, histidine, urocanic acid) and for the sweat constituents lactate and urea. A detailed description is given of the signal analysis methodology that enables the extraction of this information from the skin Raman spectra. No other noninvasive in vivo method exists that enables an analysis of skin molecular composition as a function of distance to the skin surface with similar detail and spatial resolution. Therefore, it may be expected that in vivo confocal Raman spectroscopy will find many applications in basic and applied dermatologic research.
The complementary value of analytical elemental and molecular data for archaeological relics recovered from a burial deposition is illustrated here with the non‐destructive analysis of bone specimens ...from an early human ancestor, known as Mrs Ples. Discovered in 1947 by Robert Broom in the Sterkfontein Cave system, forming part of the Cradle of Humankind in Gauteng Province, South Africa, a skull from Australopithecus africanus dated to 2.1 Mya has been identified as a Hominidae precursor to Homo sapiens. The ability to record novel compositional and structural data from such specimens without any form of chemical or mechanical pretreatment is fundamentally necessary for the preservation of such unique relics while affording conservators the information needed to undertake restoration and the adoption of conservation strategies. Our analyses revealed information about the state of preservation of the samples as well as their depositional context. Raman microspectroscopic mapping produced data on the spatial distribution of phosphate, quartz, carbon, calcite and manganese dioxide signatures on the bone fragments. Extensive deposits of pyrolusite, manganese(II) oxide, on the bone specimens are attributed to hydrogeological processes from ground water irrigation of the skeletal remains in the depositional environment; the identification of distinct quartz‐ and calcite‐rich areas may arise from taphonomic and/or depositional processes. Raman spectroscopic signatures of the organic proteinaceous component of the prehistoric bone are not observed, indicating the degradation of endogenous organics or, if present, were masked by exogeneous materials. The identification of carbon could additionally reflect the remains of biodegradative processes that have operated in the burial site. Elemental mapping using X‐ray fluorescence and variable pressure scanning electron microscopy imaging and spatially resolved energy dispersive X‐ray spectroscopy provided complementary information about the samples. The result is a comprehensive characterisation of the samples' morphology, elemental and molecular composition from the micro scale to the macroscale, using entirely non‐destructive analytical techniques.
The complementary value of analytical elemental and molecular data for archaeological relics recovered from a burial deposition is illustrated here with the non‐destructive analysis of bone specimens from an early human ancestor. The ability to record novel compositional and structural data from such specimens without any form of chemical or mechanical pretreatment is fundamentally necessary for the preservation of such unique relics while affording conservators the information needed to undertake restoration and the adoption of conservation strategies.
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