Raman spectroscopy is one of the most powerful experimental tools to study graphene, since it provides much useful information for sample characterization. In this Account, we show that this ...technique is also convenient to study other bidimensional materials beyond graphene, and we will focus on the semiconducting transition metal dichalcogenides (MX2), specifically on MoS2 and WS2. We start by comparing the atomic structure of graphene and 2H-MX2 as a function of the number of layers in the sample. The first-order Raman active modes of each material can be predicted on the basis of their corresponding point-group symmetries. We show the analogies between graphene and 2H-MX2 in their Raman spectra. Using several excitation wavelengths in the visible range, we analyze the first- and second-order features presented by each material. These are the E2g and 2TO(K) bands in graphene (also known as the G and 2D bands, respectively) and the A1′, E′, and 2LA(M) bands in 2H MX2. The double-resonance processes that originate the second-order bands are different for both systems, and we will discuss them in terms of the different electronic structure and phonon dispersion curves presented by each compound. According to the electronic structure of graphene, which is a zero band gap semiconductor, the Raman spectrum is resonant for all the excitation wavelengths. Moreover, due to the linear behavior of the electronic dispersion near the K point, the double-resonance bands of graphene are dispersive, since their frequencies vary when we change the laser energy used for the sample excitation. In contrast, the semiconducting MX2 materials present an excitonic resonance at the direct gap, and consequently, the double-resonance Raman bands of MX2 are not dispersive, and only their intensities depend on the laser energy. In this sense, resonant Raman scattering experiments performed in transition metal dichalcogenides using a wide range of excitation energies can provide information about the electronic structure of these materials, which is complementary to other optical spectroscopies, such as absorption or photoluminescence. Raman spectroscopy can also be useful to address disorder in MX2 samples in a similar way as it is used in graphene. Both materials exhibit additional Raman features associated with phonons within the interior of the Brillouin zone that are activated by the presence of defects and that are not observed in pristine samples. Such is the case of the well-known D band of graphene. MX2 samples present analogous features that are clearly observed at specific excitation energies. The origins of these double-resonance Raman bands in MX2 are still subjects of current research. Finally, we discuss the suitability of Raman spectroscopy as a strain or doping sensor. Such applications of Raman spectroscopy are being extensively studied in the case of graphene, and considering its structural analogies with MX2 systems, we show how this technique can also be used to provide strain/doping information for transition metal dichalcogenides.
Optical coherence tomography (OCT) is a non-invasive medical imaging technology that is playing an increasing role in the routine assessment and management of patients with neuro-ophthalmic ...conditions. Its ability to characterise the optic nerve head, peripapillary retinal nerve fibre layer and cellular layers of the macula including the ganglion cell layer enables qualitative and quantitative assessment of optic nerve disease. In this review, we discuss technical features of OCT and OCT-based imaging techniques in the neuro-ophthalmic context, potential pitfalls to be aware of, and specific applications in more common neuro-ophthalmic conditions including demyelinating, inflammatory, ischaemic and compressive optic neuropathies, optic disc drusen and raised intracranial pressure. We also review emerging applications of OCT angiography within neuro-ophthalmology.
Authorship Attribution for Social Media Forensics Rocha, Anderson; Scheirer, Walter J.; Forstall, Christopher W. ...
IEEE transactions on information forensics and security,
01/2017, Letnik:
12, Številka:
1
Journal Article
Recenzirano
The veil of anonymity provided by smartphones with pre-paid SIM cards, public Wi-Fi hotspots, and distributed networks like Tor has drastically complicated the task of identifying users of social ...media during forensic investigations. In some cases, the text of a single posted message will be the only clue to an author's identity. How can we accurately predict who that author might be when the message may never exceed 140 characters on a service like Twitter? For the past 50 years, linguists, computer scientists, and scholars of the humanities have been jointly developing automated methods to identify authors based on the style of their writing. All authors possess peculiarities of habit that influence the form and content of their written works. These characteristics can often be quantified and measured using machine learning algorithms. In this paper, we provide a comprehensive review of the methods of authorship attribution that can be applied to the problem of social media forensics. Furthermore, we examine emerging supervised learning-based methods that are effective for small sample sizes, and provide step-by-step explanations for several scalable approaches as instructional case studies for newcomers to the field. We argue that there is a significant need in forensics for new authorship attribution algorithms that can exploit context, can process multi-modal data, and are tolerant to incomplete knowledge of the space of all possible authors at training time.
Cancer nanotechnology is a prolific field of research, where nanotools are employed to diagnose and treat cancer with unprecedented precision. Targeted drug delivery is fundamental for more efficient ...cancer treatments. For this, nanoparticles have been extensively used during the past few years in order to improve the specificity, selectivity and controlled release of drug delivery. It holds potential in minimizing systemic toxicity through the development of functionalized particles for targeted treatment. Among all the type of nanoparticles, dendrimers display several advantages, which make them ideal candidates for improved and targeted drug delivery in cancer research. Dendrimers can transport large amounts of drug into specific areas. In addition, they can be employed for monitoring the progress of the treatment process, with an unprecedented theranostic capability. Special emphasis is given to colorectal cancer and to the preferred employed strategies for producing drug-loaded/functionalized NPs for cancer therapy in the past few years.
► Predictions for radio pulses emitted by extensive air showers obtained in a full MC simulation (ZHAireS). ► Results compatible with superposition of geomagnetic and charge excess radio emission. ► ...Geometry coupled to relativistic effects play prominent role. ► Toy model developed. Variable refractive index.
We present predictions for the radio pulses emitted by extensive air showers using ZHAireS, an AIRES-based Monte Carlo code that takes into account the full complexity of ultra-high energy cosmic-ray induced shower development in the atmosphere, and allows the calculation of the electric field in both the time and frequency domains. We do not presuppose any emission mechanism and our results are compatible with a superposition of geomagnetic and charge excess radio emission effects. We investigate the polarization of the electric field as well as the effects of the refractive index
n and shower geometry on the radio pulses. We show that geometry, coupled to the relativistic effects that appear when using a realistic refractive index
n
>
1, play a prominent role on the radio emission of air showers.
To characterize the sequential events that are taking place in retinal neurodegeneration in a murine model of spontaneous type 2 diabetes (db/db mouse).
C57BLKsJ-db/db mice were used as spontaneous ...type 2 diabetic animal model, and C57BLKsJ-db/+ mice served as the control group. To assess the chronological sequence of the abnormalities the analysis was performed at different ages (8, 16 and 24 weeks). The retinas were evaluated in terms of morphological and functional abnormalities electroretinography (ERG). Histological markers of neurodegeneration (glial activation and apoptosis) were evaluated by immunohistochemistry. In addition glutamate levels and glutamate/aspartate transporter (GLAST) expression were assessed. Furthermore, to define gene expression changes associated with early diabetic retinopathy a transcriptome analyses was performed at 8 week. Furthermore, an additional interventional study to lower blood glucose levels was performed.
Glial activation was higher in diabetic than in non diabetic mice in all the stages (p<0.01). In addition, a progressive loss of ganglion cells and a significant reduction of neuroretinal thickness were also observed in diabetic mice. All these histological hallmarks of neurodegeneration were less pronounced at week 8 than at week 16 and 24. Significant ERG abnormalities were present in diabetic mice at weeks 16 and 24 but not at week 8. Moreover, we observed a progressive accumulation of glutamate in diabetic mice associated with an early downregulation of GLAST. Morphological and ERG abnormalities were abrogated by lowering blood glucose levels. Finally, a dysregulation of several genes related to neurotransmission and oxidative stress such as UCP2 were found at week 8.
Our results suggest that db/db mouse reproduce the features of the neurodegenerative process that occurs in the human diabetic eye. Therefore, it seems an appropriate model for investigating the underlying mechanisms of diabetes-induced retinal neurodegeneration and for testing neuroprotective drugs.
ABSTRACT
We have investigated the structure of the Hercules supercluster (SCL160) based on data originally extracted from the Sloan Digital Sky Survey (SDSS-DR7). We have traced the mass distribution ...in the field through the numerical density, weighted by the r′ luminosity of the galaxies, and classified objects based on their spatial position and redshift. This has allowed us to address not only the kinematics of the supercluster as a whole, but also the internal kinematics of each cluster, which had not been explored further before. We have confirmed that the Hercules supercluster is composed of the galaxy clusters A2147, A2151, and A2152. A2151 consists of five subclusters, A2147 two, and A2152 at least two. They form the heart of the Hercules supercluster. We also have found two other gravitationally bound clusters, therefore increasing the known members of the supercluster. We have estimated a total mass of 2.1 ± 0.2 × 1015 M⊙ for the Hercules supercluster. To determine the dynamical masses in this work, we have resorted to the M200−σ scaling relation and the caustic technique. Comparing both methods with simulated data of bimodal merging clusters, we found that the caustic, as well as the σ-based masses, is biased through the merger age, showing a boost just after pericentric passage. This is not in line with the principle of the caustic method, which affirms it does not depend on the cluster dynamical state.
The Various Roles of Fatty Acids de Carvalho, Carla C C R; Caramujo, Maria José
Molecules (Basel, Switzerland),
10/2018, Letnik:
23, Številka:
10
Journal Article
Recenzirano
Odprti dostop
Lipids comprise a large group of chemically heterogeneous compounds. The majority have fatty acids (FA) as part of their structure, making these compounds suitable tools to examine processes raging ...from cellular to macroscopic levels of organization. Among the multiple roles of FA, they have structural functions as constituents of phospholipids which are the "building blocks" of cell membranes; as part of neutral lipids FA serve as storage materials in cells; and FA derivatives are involved in cell signalling. Studies on FA and their metabolism are important in numerous research fields, including biology, bacteriology, ecology, human nutrition and health. Specific FA and their ratios in cellular membranes may be used as biomarkers to enable the identification of organisms, to study adaptation of bacterial cells to toxic compounds and environmental conditions and to disclose food web connections. In this review, we discuss the various roles of FA in prokaryotes and eukaryotes and highlight the application of FA analysis to elucidate ecological mechanisms. We briefly describe FA synthesis; analyse the role of FA as modulators of cell membrane properties and FA ability to store and supply energy to cells; and inspect the role of polyunsaturated FA (PUFA) and the suitability of using FA as biomarkers of organisms.
Recent improvements in the speed, cost and accuracy of next generation sequencing are revolutionizing the discovery of single nucleotide polymorphisms (SNPs). SNPs are increasingly being used as an ...addition to the molecular ecology toolkit in nonmodel organisms, but their efficient use remains challenging. Here, we discuss common issues when employing SNP markers, including the high numbers of markers typically employed, the effects of ascertainment bias and the inclusion of nonneutral loci in a marker panel. We provide a critique of considerations specifically associated with the application and population genetic analysis of SNPs in nonmodel taxa, focusing specifically on some of the most commonly applied methods.
The hypothesis that lung collapse is detrimental during the acute respiratory distress syndrome is still debatable. One of the difficulties is the lack of an efficient maneuver to minimize it.
To ...test if a bedside recruitment strategy, capable of reversing hypoxemia and collapse in > 95% of lung units, is clinically applicable in early acute respiratory distress syndrome.
Prospective assessment of a stepwise maximum-recruitment strategy using multislice computed tomography and continuous blood-gas hemodynamic monitoring.
Twenty-six patients received sequential increments in inspiratory airway pressures, in 5 cm H(2)O steps, until the detection of Pa(O(2)) + Pa(CO(2)) >or= 400 mm Hg. Whenever this primary target was not met, despite inspiratory pressures reaching 60 cm H(2)O, the maneuver was considered incomplete. If there was hemodynamic deterioration or barotrauma, the maneuver was to be interrupted. Late assessment of recruitment efficacy was performed by computed tomography (9 patients) or by online continuous monitoring in the intensive care unit (15 patients) up to 6 h. It was possible to open the lung and to keep the lung open in the majority (24/26) of patients, at the expense of transient hemodynamic effects and hypercapnia but without major clinical consequences. No barotrauma directly associated with the maneuver was detected. There was a strong and inverse relationship between arterial oxygenation and percentage of collapsed lung mass (R = - 0.91; p < 0.0001).
It is often possible to reverse hypoxemia and fully recruit the lung in early acute respiratory distress syndrome. Due to transient side effects, the required maneuver still awaits further evaluation before routine clinical application.
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