Summary
A clearer understanding of the tumor immune microenvironment (TIME) in metastatic clear cell renal cell carcinoma (ccRCC) may help to inform precision treatment strategies. We sought to ...identify clinically meaningful TIME signatures in ccRCC. We studied tumors from 39 patients with metastatic ccRCC using quantitative multiplexed immunofluorescence and relevant immune marker panels. Cell densities were analyzed in three regions of interest (ROIs): tumor core, tumor–stroma interface and stroma. Patients were stratified into low‐ and high‐marker density groups using median values as thresholds. Log‐rank and Cox regression analyses while controlling for clinical variables were used to compare survival outcomes to patterns of immune cell distributions. There were significant associations with increased macrophage (CD68+CD163+CD206+) density and poor outcomes across multiple ROIs in primary and metastatic tumors. In primary tumors, T‐bet+ T helper type 1 (Th1) cell density was highest at the tumor–stromal interface (P = 0·0021), and increased co‐expression of CD3 and T‐bet was associated with improved overall survival (P = 0·015) and survival after immunotherapy (P = 0·014). In metastatic tumor samples, decreased forkhead box protein 3 (FoxP3)+ T regulatory cell density correlated with improved survival after immunotherapy (P = 0·016). Increased macrophage markers and decreased Th1 T cell markers within the TIME correlated with poor overall survival and treatment outcomes. Immune markers such as FoxP3 showed consistent levels across the TIME, whereas others, such as T‐bet, demonstrated significant variance across the distinct ROIs. These findings suggest that TIME profiling outside the tumor core may identify clinically relevant associations for patients with metastatic ccRCC.
A better understanding of the tumor immune microenvironment may serve as a critical step in advancing precision treatment strategies. We analyzed tumors from patients with metastatic clear cell renal cell carcinoma using quantitative multiplexed immunofluorescence and relevant immune cell markers in three separate regions of interest. Our results demonstrate that patterns of immune cell spatial distribution across microenvironment compartments in primary and metastatic tumors correlate with meaningful clinical outcomes.
The first international meeting of the Archaeozoology of Southwest Asia and Adjacent Areas (ASWA) working group of the International Council for Archaeozoology (ICAZ) took place at the University of ...Groningen in 1992. Ever since, ASWA meetings have served as an inspiring gathering for those conducting archaeozoological research in Southwest Asia, the Eastern Mediterranean, North Africa, Central Asia and the Caucasus. This book contains sixteen papers presented at the 12th ASWA meeting hosted at its inaugural institution, the University of Groningen, Groningen Institute of Archaeology, as a continuation of the usual series and to celebrate the career of Dr. Hijlke Buitenhuis, associated member and alumnus of the institute, co-organizer of the first ASWA meeting.Like other ASWA proceedings before it, this volume is full of novel theoretical and methodological approaches and new research results, tackling a large variety of topics, from the geometric morphometrics of sheep in the Pre-Pottery Neolithic Period to Predynastic fishing in the Upper Nile, to the biogeography of hartebeest and hemione, and covering the vast region stretching between Hungary in the west and Azerbaijan in the east. The volume also features an opening article by ASWA founding member M.A. Zeder on the future of archaeozoology in the region. In honor of Dr. Hijlke Buitenhuis, his full bibliography is featured herein.
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