RNA silencing suppressors from different plant viruses are structurally diverse. In addition to inhibiting the antiviral silencing response to condition susceptibility, many suppressors are ...pathogenicity factors that cause disease or developmental abnormalities. Here, unrelated suppressors from multiple viruses were shown to inhibit microRNA (miRNA) activities and trigger an overlapping series of severe developmental defects in transgenic Arabidopsis thaliana. This suggests that interference with miRNA-directed processes may be a general feature contributing to pathogenicity of many viruses. A normally labile intermediate in the miRNA biogenesis/RNA-induced silencing complex (RISC) assembly pathway, miRNA*, accumulated specifically in the presence of suppressors (P1/HC-Pro, p21, or p19) that inhibited miRNA-guided cleavage of target mRNAs. Both p21 and p19, but not P1/HC-Pro, interacted with miRNA/miRNA* complexes and hairpin RNA-derived short interfering RNAs (siRNAs) in vivo. In addition, p21 bound to synthetic miRNA/miRNA* and siRNA duplexes in vitro. We propose that several different suppressors act by distinct mechanisms to inhibit the incorporation of small RNAs into active RISCs.
This study aimed to characterize antimicrobial resistance and virulence genes in multi-drug resistant enterotoxigenic
Escherichia coli (ETEC) isolates (
n
=
117) collected from porcine post-weaning ...diarrhoea cases in Australia (1999–2005). Isolates were serotyped, antibiogram-phenotyped for 12 antimicrobial agents and genotyped by PCR for 30 plasmid-mediated antimicrobial resistance genes (ARGs), 22 intestinal and 38 extraintestinal
E. coli virulence genes (VGs). Nine serogroups were identified, the most prevalent being O149 (46.2%), O141 (11.2%) and Ont (31.6%). None of the isolates showed resistance to ceftiofur or enrofloxacin and 9.4% were resistant to florfenicol. No corresponding extended-spectrum/AmpC β-lactamase, fluoroquinolone or
floR ARGs were detected. An antimicrobial resistance index (ARI) was calculated from the combined data with a weighting for each antimicrobial agent dependent upon its significance to human health. Serogroup O141 isolates had a significantly higher ARI due to an elevated prevalence of aminoglycoside ARGs and possession of more virulence genes (VGs), including ExPEC or EHEC adhesins (
bmaE,
sfa/focDE,
fimH,
ihA) in toxin-producing strains that lacked the normally associated F4 and F18 fimbriae. Few associations between ARGs and VGs were apparent, apart from
tetC,
sfa/focDE and
ompT which, for a sub-set of O141 isolates, suggest possible plasmid acquisition from ExPEC. The multi-drug resistant ETEC ARG/VG profiles indicate a high probability of considerable strain and plasmid diversity, reflecting various selection pressures at the individual farm level rather than emergence and lateral spread of MDR resistant/virulent clones.
Qualitative studies remain relatively uncommon in the transplant literature but are an important approach contributing unique strengths in some areas of research. With the increased focus on ...patient‐centered research and decision‐making, it is timely to review qualitative research in the context of transplantation. While quantitative research addresses questions about the effectiveness of interventions or associations between risk factors and outcomes, qualitative research has an equal and complementary role in providing understanding about people's behaviors, attitudes, and values. Qualitative research has provided insights into some of the important but elusive questions in transplantation, including the sources of barriers to organ donation and inequities in access to transplantation, nonadherence to immunosuppressive regimens, and complex psychosocial outcomes. This review highlights recent contributions of qualitative research to transplantation practice and policy, and identifies key principles to guide qualitative research appraisal.
Qualitative research provides insights into some of the important but elusive questions in transplantation, and this review highlights recent contributions of qualitative research to transplantation practice and policy, identifying key principles to guide qualitative research appraisal.
Betatron radiation from laser wakefield accelerators is an ultrashort pulsed source of hard, synchrotron-like x-ray radiation. It emanates from a centimetre scale plasma accelerator producing GeV ...level electron beams. In recent years betatron radiation has been developed as a unique source capable of producing high resolution x-ray images in compact geometries. However, until now, the short pulse nature of this radiation has not been exploited. This report details the first experiment to utilize betatron radiation to image a rapidly evolving phenomenon by using it to radiograph a laser driven shock wave in a silicon target. The spatial resolution of the image is comparable to what has been achieved in similar experiments at conventional synchrotron light sources. The intrinsic temporal resolution of betatron radiation is below 100 fs, indicating that significantly faster processes could be probed in future without compromising spatial resolution. Quantitative measurements of the shock velocity and material density were made from the radiographs recorded during shock compression and were consistent with the established shock response of silicon, as determined with traditional velocimetry approaches. This suggests that future compact betatron imaging beamlines could be useful in the imaging and diagnosis of high-energy-density physics experiments.
Extraordinary states of highly localised pressure and temperature can be generated upon the collapse of impulsively driven cavities. Direct observation of this phenomenon in solids has proved ...challenging, but recent advances in high-speed synchrotron radiography now permit the study of highly transient, subsurface events in real time. We present a study on the shock-induced collapse of spherical cavities in a solid polymethyl methacrylate medium, driven to shock states between 0.49 and 16.60 GPa. Utilising multi-MHz phase contrast radiography, extended sequences of the collapse process have been captured, revealing new details of interface motion, material failure and jet instability formation. Results reveal a rich array of collapse characteristics dominated by strength effects at low shock pressures and leading to a hydrodynamic response at the highest loading conditions.
We use observations from the Boolardy Engineering Test Array (BETA) of the Australian Square Kilometre Array Pathfinder (ASKAP) telescope to search for transient radio sources in the field around the ...intermittent pulsar PSR J1107−5907. The pulsar is thought to switch between an ‘off’ state in which no emission is detectable, a weak state and a strong state. We ran three independent transient detection pipelines on two-minute snapshot images from a 13 h BETA observation in order to (1) study the emission from the pulsar, (2) search for other transient emission from elsewhere in the image and (3) to compare the results from the different transient detection pipelines. The pulsar was easily detected as a transient source and, over the course of the observations, it switched into the strong state three times giving a typical time-scale between the strong emission states of 3.7 h. After the first switch it remained in the strong state for almost 40 min. The other strong states lasted less than 4 min. The second state change was confirmed using observations with the Parkes radio telescope. No other transient events were found and we place constraints on the surface density of such events on these time-scales. The high sensitivity Parkes observations enabled us to detect individual bright pulses during the weak state and to study the strong state over a wide observing band. We conclude by showing that future transient surveys with ASKAP will have the potential to probe the intermittent pulsar population.
The nature of the transition from acute to chronic pain still eludes explanation, but chronic pain resulting from surgery provides a natural experiment that invites clinical epidemiological ...investigation and basic scientific inquiry into the mechanisms of this transition. The primary purpose of this article is to review current knowledge and hypotheses on the transition from acute to persistent postsurgical pain, summarizing literature on clinical epidemiological studies of persistent postsurgical pain development, as well as basic neurophysiological studies targeting mechanisms in the periphery, spinal cord, and brain. The second purpose of this article is to integrate theory, information, and causal reasoning in these areas. Conceptual mapping reveals 5 classes of hypotheses pertaining to pain. These propose that chronic pain results from: 1) persistent noxious signaling in the periphery; 2) enduring maladaptive neuroplastic changes at the spinal dorsal horn and/or higher central nervous system structures reflecting a multiplicity of factors, including peripherally released neurotrophic factors and interactions between neurons and microglia; 3) compromised inhibitory modulation of noxious signaling in medullary-spinal pathways; 4) descending facilitatory modulation; and 5) maladaptive brain remodeling in function, structure, and connectivity. The third purpose of this article is to identify barriers to progress and review opportunities for advancing the field. This review reveals a need for a concerted, strategic effort toward integrating clinical epidemiology, basic science research, and current theory about pain mechanisms to hasten progress toward understanding, managing, and preventing persistent postsurgical pain.
The development of chronic pain after surgery is a major clinical problem that provides an opportunity to study the transition from acute to chronic pain at epidemiologic and basic science levels. Strategic, coordinated, multidisciplinary research efforts targeting mechanisms of pain chronification can to help minimize or eliminate persistent postsurgical pain.
Blade Element Momentum Theory (BEMT) is a computationally efficient method of calculating the performance of a tidal stream turbine (TST) generating energy from the ocean. This efficiency is achieved ...by making several simplifying assumptions; an unintended consequence of these assumptions is the omission of some phenomena that can significantly alter the performance and loads of a TST. We can ameliorate this by incorporating suitable corrections into a BEMT model, which allow us to account for some of the effects of these phenomena. This paper examines the implementation of corrections in an established BEMT solver for two such phenomena: tip/hub losses and high induction conditions.
Tip losses are attributable to the flow of fluid around end of the blade, a flow feature omitted in the classical BEMT treatment of turbines. At high tip speed ratios, above the designed operating range of the device, the theory based on an axial interference factor, a, diverges from experimental results and, indeed, becomes physically untenable. Buhl proposed a high induction correction factor for wind turbines operating in air and a modified version of his correction is implemented here for a TST operating in water. The tip/hub loss and high-induction corrections are well-integrated with one another. The validity of the high-induction correction is checked against experimental results; we find that our model predicts power output well but overpredicts axial thrust compared to laboratory observations.
•We model tidal turbines using blade element momentum theory (BEMT).•The BEMT model includes corrections for tip/hub losses and high-induction effects.•The high-induction correction is validated against experimental data.•We make predictions for a full-scale rotor operated in a high-induction regime.
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