ABSTRACT
Accreting black holes produce collimated outflows, or jets, that traverse many orders of magnitude in distance, accelerate to relativistic velocities, and collimate into tight opening ...angles. Of these, perhaps the least understood is jet collimation due to the interaction with the ambient medium. In order to investigate this interaction, we carried out axisymmetric general relativistic magnetohydrodynamic simulations of jets produced by a large accretion disc, spanning over 5 orders of magnitude in time and distance, at an unprecedented resolution. Supported by such a disc, the jet attains a parabolic shape, similar to the M87 galaxy jet, and the product of the Lorentz factor and the jet half-opening angle, γθ ≪ 1, similar to values found from very long baseline interferometry (VLBI) observations of active galactic nuclei (AGNs) jets; this suggests extended discs in AGNs. We find that the interaction between the jet and the ambient medium leads to the development of pinch instabilities, which produce significant radial and lateral variability across the jet by converting magnetic and kinetic energy into heat. Thus pinched regions in the jet can be detectable as radiating hotspots and may provide an ideal site for particle acceleration. Pinching also causes gas from the ambient medium to become squeezed between magnetic field lines in the jet, leading to enhanced mass loading and deceleration of the jet to non-relativistic speeds, potentially contributing to the spine-sheath structure observed in AGN outflows.
Abstract
Magnetic reconnection can power bright, rapid flares originating from the inner magnetosphere of accreting black holes. We conduct extremely high-resolution (5376 × 2304 × 2304 cells) ...general-relativistic magnetohydrodynamics simulations, capturing plasmoid-mediated reconnection in a 3D magnetically arrested disk for the first time. We show that an equatorial, plasmoid-unstable current sheet forms in a transient, nonaxisymmetric, low-density magnetosphere within the inner few Schwarzschild radii. Magnetic flux bundles escape from the event horizon through reconnection at the universal plasmoid-mediated rate in this current sheet. The reconnection feeds on the highly magnetized plasma in the jets and heats the plasma that ends up trapped in flux bundles to temperatures proportional to the jet’s magnetization. The escaped flux bundles can complete a full orbit as low-density hot spots, consistent with Sgr A* observations by the GRAVITY interferometer. Reconnection near the horizon produces sufficiently energetic plasma to explain flares from accreting black holes, such as the TeV emission observed from M87. The drop in the mass accretion rate during the flare and the resulting low-density magnetosphere make it easier for very-high-energy photons produced by reconnection-accelerated particles to escape. The extreme-resolution results in a converged plasmoid-mediated reconnection rate that directly determines the timescales and properties of the flare.
Abstract
We evolve two high-resolution general relativistic magnetohydrodynamic simulations of advection-dominated accretion flows around nonspinning black holes (BHs), each over a duration ∼3 × 10
5
...GM
BH
/
c
3
. One model captures the evolution of a weakly magnetized (SANE) disk and the other that of a magnetically arrested disk (MAD). Magnetic flux eruptions in the MAD model push out gas from the disk and launch strong winds with outflow efficiencies at times reaching 10% of the incoming accretion power. Despite the substantial power in these winds, average mass outflow rates remain low out to a radius ∼100
GM
BH
/
c
2
, only reaching ∼60%–80% of the horizon accretion rate. The average outward angular momentum transport is primarily radial in both modes of accretion, but with a clear distinction: magnetic flux eruption–driven disk winds cause a strong vertical flow of angular momentum in the MAD model, while for the SANE model, the magnetorotational instability (MRI) moves angular momentum mostly equatorially through the disk. Further, we find that the MAD state is highly transitory and nonaxisymmetric, with the accretion mode often changing to a SANE-like state following an eruption before reattaining magnetic flux saturation with time. The Reynolds stress changes directions during such transitions, with the MAD (SANE) state showing an inward (outward) stress, possibly pointing to intermittent MRI-driven accretion in MADs. Pinning down the nature of flux eruptions using next-generation telescopes will be crucial in understanding the flow of mass, magnetic flux, and angular momentum in sub-Eddington accreting BHs like M87* and Sagittarius A*.
Fluorescent labels have been widely used for biological applications, primarily in imaging and assays. Traditional fluorophores such as fluorescent dyes are mainly based on downconversion ...fluorescence, which have several drawbacks such as photobleaching, high background noise from autofluorescence, and considerable photodamage to biological materials. Upconverting fluorescent nanoparticles emit detectable photons of higher energy in the near‐infrared (NIR) or visible range upon irradiation with an NIR light in a process termed ‘upconversion.’ They overcome some of the disadvantages faced by conventional downconversion labels, thus making them an ideal fluorescent label for biological applications. This review looks at the development of these particles, critically examines the reported applications, and discusses their future in biomedicine.
Upconverting fluorescent nanoparticles emit detectable photons of higher energy in the near‐infrared (NIR) or visible range upon irradiation with an NIR light in a process termed ‘upconversion.’ They overcome some of the disadvantages faced by conventional downconversion labels, thus making them an ideal fluorescent label for biological applications. The lead‐in photograph depicts rare‐earth upconversion nanoparticles and their use in cell imaging.
Virstatin is a small molecule that inhibits Vibrio cholerae virulence regulation, the causative agent for cholera. Here we report the interaction of virstatin with human serum albumin (HSA) using ...various biophysical methods. The drug binding was monitored using different isomeric forms of HSA (N form ∼pH 7.2, B form ∼pH 9.0 and F form ∼pH 3.5) by absorption and fluorescence spectroscopy. There is a considerable quenching of the intrinsic fluorescence of HSA on binding the drug. The distance (r) between donor (Trp214 in HSA) and acceptor (virstatin), obtained from Forster-type fluorescence resonance energy transfer (FRET), was found to be 3.05 nm. The ITC data revealed that the binding was an enthalpy-driven process and the binding constants K(a) for N and B isomers were found to be 6.09×10(5 )M(-1) and 4.47×10(5) M(-1), respectively. The conformational changes of HSA due to the interaction with the drug were investigated from circular dichroism (CD) and Fourier Transform Infrared (FTIR) spectroscopy. For 1:1 molar ratio of the protein and the drug the far-UV CD spectra showed an increase in α- helicity for all the conformers of HSA, and the protein is stabilized against urea and thermal unfolding. Molecular docking studies revealed possible residues involved in the protein-drug interaction and indicated that virstatin binds to Site I (subdomain IIA), also known as the warfarin binding site.
Abstract Upconversion fluorescence imaging technique with excitation in the near-infrared (NIR) region has been used for imaging of biological cells and tissues. This has several advantages, ...including absence of photo-damage to living organisms, very low auto-fluorescence, high detection sensitivity, and high light penetration depth in biological tissues. In this report we demonstrate the use of a new upconversion fluorophore, lanthanide doped nanocrystals, for imaging of cells and some deep tissues in animal. Polyethyleneimine (PEI) coated NaYF4 :Yb,Er nanoparticles were synthesized, which produce very strong upconversion fluorescence when excited at 980 nm by a NIR laser. The nanoparticles were shown to be stable in physiologic buffered saline (PBS), non-toxic to bone marrow stem cells, and resistant to photo-bleaching. The nanoparticles delivered into some cell lines or injected intradermally and intramuscularly into some tissues either near the body surface or deep in the body of rats showed visible fluorescence, when exposed to a 980 nm NIR laser. To the best of our knowledge, this represents the first demonstration of use of upconversion fluorophores for cellular and tissue imaging.
Decarboxylative borylation Li, Chao; Wang, Jie; Barton, Lisa M. ...
Science (American Association for the Advancement of Science),
06/2017, Letnik:
356, Številka:
6342
Journal Article
Recenzirano
Odprti dostop
The widespread use of alkyl boronic acids and esters is frequently hampered by the challenges associated with their preparation. We describe a simple and practical method to rapidly access densely ...functionalized alkyl boronate esters from abundant carboxylic substituents. This broad-scope nickel-catalyzed reaction uses the same activating principle as amide bond formation to replace a carboxylic acid moiety with a boronate ester. Application to peptides allowed expedient preparations of α-amino boronic acids, often with high stereoselectivity, thereby facilitating synthesis of the alkyl boronic acid drugs Velcade and Ninlaro as well as a boronic acid version of the iconic antibiotic vancomycin. The reaction also enabled the discovery and extensive biological characterization of potent human neutrophil elastase inhibitors, which offer reversible covalent binding properties.
ABSTRACT
Sgr A* exhibits regular variability in its multiwavelength emission, including daily X-ray flares and roughly continuous near-infrared (NIR) flickering. The origin of this variability is ...still ambiguous since both inverse Compton and synchrotron emission are possible radiative mechanisms. The underlying particle distributions are also not well constrained, particularly the non-thermal contribution. In this work, we employ the GPU-accelerated general relativistic magnetohydrodynamics code H-AMR to perform a study of flare flux distributions, including the effect of particle acceleration for the first time in high-resolution 3D simulations of Sgr A*. For the particle acceleration, we use the general relativistic ray-tracing code bhoss to perform the radiative transfer, assuming a hybrid thermal+non-thermal electron energy distribution. We extract ∼60 h light curves in the sub-millimetre, NIR and X-ray wavebands, and compare the power spectra and the cumulative flux distributions of the light curves to statistical descriptions for Sgr A* flares. Our results indicate that non-thermal populations of electrons arising from turbulence-driven reconnection in weakly magnetized accretion flows lead to moderate NIR and X-ray flares and reasonably describe the X-ray flux distribution while fulfilling multiwavelength flux constraints. These models exhibit high rms per cent amplitudes, $\gtrsim 150{{\ \rm per\ cent}}$ both in the NIR and the X-rays, with changes in the accretion rate driving the 230 GHz flux variability, in agreement with Sgr A* observations.
Microbial Symbiosis: A Network towards Biomethanation Saha, Shouvik; Basak, Bikram; Hwang, Jae-Hoon ...
Trends in microbiology (Regular ed.),
December 2020, 2020-12-00, 20201201, Letnik:
28, Številka:
12
Journal Article
Recenzirano
Biomethanation through anaerobic digestion (AD) is the most reliable energy harvesting process to achieve waste-to-energy. Microbial communities, including hydrolytic and fermentative bacteria, ...syntrophic bacteria, and methanogenic archaea, and their interspecies symbioses allow complex metabolisms for the volumetric reduction of organic waste in AD. However, heterogeneity in organic waste induces community shifts in conventional anaerobic digesters treating sewage sludge at wastewater treatment plants globally. Assessing the metabolic roles of individual microbial species in syntrophic communities remains a challenge, but such information has important implications for microbially enhanced energy recovery. This review focuses on the alterations in digester microbiome and intricate interspecies networks during substrate variation, symbiosis among the populations, and their implications for biomethanation to aid stable operation in real-scale digesters.
Anaerobic digestion (AD) involves hydrolysis and acidogenesis, acetogenesis, interspecies electron transfers, and methanogenesis.Heterogeneity in organic waste induces community shifts in digester microbiota during AD.Community shifts affect intricate interspecies symbiotic networks and stable biomethanation.Alterations in deterministic factors influence the microbiota, leading to digester perturbation.
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