Pericarpium Citri Reticulatae 'Chachiensis' (PCRC), the premium aged pericarps of Pericarpium Citri Reticulatae, is widely used in traditional Chinese medicines with a diversity of promising ...bioactivity. Herein we report the extraction, characterization and underlying mechanism of anti-metabolic syndrome of an arabinan-rich polysaccharide from PCRC (PCRCP). This polysaccharide was obtained in a 7.0% yield by using ultrasound-assisted extraction under the optimized conditions of 30 mL/g liquid-to-solid ratio, 250 W ultrasound power for 20 min at 90 °C with pH 4.5. The PCRCP with an average molecular weight of 122.0 kDa, is mainly composed of D-galacturonic acid, arabinose and galactose, which may link via 1,4-linked Gal(p)-UA, 1,4-linked Ara(f) and 1,4-linked Gal(p). Supplementation with PCRCP not only effectively alleviated the weight gain, adiposity and hyperglycemia, but also regulated the key metabolic pathways involved in the de novo synthesis and β-oxidation of fatty acid in high-fat diet (HFD)-fed mice. Furthermore, PCRCP treatment caused a significant normalization in the intestinal barrier and composition of gut microbiota in mice fed by HFD. Notably, PCRCP selectively enriched Lactobacillus johnsonii at the family-genus-species levels, a known commensal bacterium, the level of which was decreased in mice fed by HFD. The depletion of microbiome induced by antibiotics, significantly compromised the effects of anti-metabolic syndrome of PCRCP in mice fed by HFD, demonstrating that the protective phenotype of PCRCP against anti-obesity is dependent on gut microbiota. PCRCP is exploitable as a potential prebiotic for the intervention of obesity and its complications.
Polymethoxyflavonoids (PMFs), the main bioactive compounds naturally occurring in the pericarp of
'Chachi' (CRCP), possess significant antitumor action. However, the action of PMFs in nasopharyngeal ...carcinoma (NPC) is currently unknown. The present research study was conducted to investigate the inhibitory mechanisms of PMFs from CRCP on NPC growth
and
. In our research, we used high-speed counter-current chromatography (HSCCC) to separate four PMFs (nobiletin (NOB), 3,5,6,7,8,3',4'-heptamethoxyflavone (HMF), tangeretin (TGN), and 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone (5-HPMF)) from CRCP. CCK-8 assay was used to preliminarily screen cell viability following exposure to the four PMFs. Colony formation, Hoechst-33258 staining, transwell, and wound scratch assays were performed to assess the anti-proliferation, invasion, migration, and apoptosis-inducing effects of HMF on NPC cells. NPC tumors in xenograft tumor transplantation experiments were also established to explore the effect of HMF (100 and 150 mg/kg/day) on NPC. The histopathological changes in the treated rats were observed by H&E staining and Ki-67 detection by immunohistochemical techniques. The expressions of P70S6K, p-P70S6K, S6, p-S6, COX-2, p53, and p-p53 were measured by Western blot. The four PMFs were obtained with high purity (>95.0%). The results of the preliminary screening by CCK-8 assay suggested that HMF had the strongest inhibitory effect on NPC cell growth. The results of the colony formation, Hoechst-33258 staining, transwell, and wound scratch assays indicated that HMF had significant anti-proliferation, invasion, migration, and apoptosis-inducing ability in NPC cells. Moreover, HMF suppressed NPC tumor growth in xenograft tumor transplantation experiments. Further investigation suggested that HMF regulated NPC cells proliferation, apoptosis, migration, and invasion by activating AMPK-dependent signaling pathways. In conclusion, HMF-induced AMPK activation inhibited NPC cell growth, invasion, and metastatic potency by downregulating the activation of the mTOR signaling pathway and COX-2 protein levels, as well as enhancing the p53 phosphorylation level. Our study provides a crucial experimental basis for the clinical treatment of NPC, as well as the development and utilization of PMFs from CRCP.
The overuse of synthetic insecticides has led to various negative consequences, including insecticide resistance, environmental pollution, and harm to public health. This may be ameliorated by using ...insecticides derived from botanical sources. The primary objective of this study was to evaluate the anti-mosquito activity of the essential oil (EO) of
Blanco cv. Chachiensis (Chachi) (referred to as CRB) at immature, semi-mature, and mature stages. The chemical compositions of the CRB EO were analyzed using GC-MS. The main components were identified to be D-limonene and
-terpinene. The contents of D-limonene at the immature, semi-mature, and mature stages were 62.35%, 76.72%, and 73.15%, respectively; the corresponding contents of
-terpinene were 14.26%, 11.04%, and 11.27%, respectively. In addition, the corresponding contents of a characteristic component, methyl 2-aminobenzoate, were 4.95%, 1.93%, and 2.15%, respectively. CRB EO exhibited significant larvicidal activity against
(
, Diptera: Culicidae), with the 50% lethal doses being 65.32, 61.47, and 65.91 mg/L for immature, semi-mature, and mature CRB EO, respectively. CRB EO was able to inhibit acetylcholinesterase and three detoxification enzymes, significantly reduce the diversity of internal microbiota in mosquitoes, and decrease the relative abundance of core species within the microbiota. The present results may provide novel insights into the utilization of plant-derived essential oils in anti-mosquitoes.
Gas chromatograph–mass spectrometer (GC–MS), ultra‐high‐performance liquid chromatograph‐Q‐Exactive Orbitrap tandem mass spectrometry (UHPLC‐Q‐Exactive Orbitrap‐MS), and high‐performance liquid ...chromatography–photodiode array detection (HPLC–PDA) were used to qualitatively and quantitatively analyze the chemical component of Citri Reticulatae Pericarpium Viride “Geqingpi” (GQP). First of all, the volatile components of GQP are identified by GC–MS. Totally 56 volatile components were determined, and γ‐Terpinene (33.39%) and D‐Limonene (22.95%) were the main terpenes. Secondly, UHPLC‐Q‐Exactive Orbitrap‐MS was used for identifying nonvolatile compositions and 42 compositions were identified totally, including 23 flavonoids, nine organic acids, three coumarins, two alkaloids compounds, and five other compounds, among which nine of the determined constituents were detected for the first time in GQP. Thirdly, the content of seven main constituents in GQP was quantitatively analyzed via HPLC‐PDA, which were synephrine, hesperidin, limonin, nobiletin, HMF, tangeretin, and 5‐HPMF. Further investigation for quantitative analysis of seven bioactive compounds suggested that the concentration of hesperidin in GQP approximately was 16.0% (160.78 ± 0.95 mg·g−1), which was far higher than the standard for identification and quality control of CRPV in Chinese Pharmacopoeia (2020 edition) that “the content of hesperidin shall not be less than 5.0%.” The phytochemicals of GQP were elucidated in this study, which might be supporting information for identification between GQP and Citri Reticulatae Pericarpium Viride “Sihuaqingpi” (SHQP) and provided a scientific basis for the further active ingredient for pharmacological research and development prospects of GQP.
Identification of volatile and nonvolatile compounds in Citri Reticulatae Pericarpium Viride using GC–MS, UPLC‐Q‐Exactive Orbitrap‐MS, and HPLC–PDA.
Citri Exocarpium Rubrum (CER), Citri Reticulatae Endocarpium Alba (CREA), and Citri Fructus Retinervus (CFR) are used as medicine and food, which derive from three different parts of the pericarp of ...Citrus reticulata Blanco through natural drying. To systematically investigate similarities and differences in phytochemicals about the three herbs, a series of analytic approaches were applied for the qualitative and quantitative analysis of chemical constituents in them. The results indicated a total of 48 volatile compounds were determined representing 99.92% of the total relative content of CER extracts, including 24 alkenes, 11 alcohols, 6 aldehydes, 2 ketones, and 2 phenols, while volatile compounds were not extracted from CREA and CFR. CER was abundant in volatile components that mainly existed in the oil gland. And a total of 32, 35, and 28 nonvolatile compounds were identified from CER, CREA, and CFR extracts, respectively. The total content of flavonoids and phenolic, and hesperidin in CFR was the highest, followed by CREA and CER. Conversely, CER was a rich source of polymethoxyflavones (PMFs), and the total polymethoxyflavone content (TPMFC), the content of nobiletin, 3,5,6,7,8,3′,4′‐heptamethoxyflavone (HMF), tangeretin, and 5‐hydroxy‐6,7,8,3′,4′‐pentamethoxyflavone (5‐HPMF) in CREA and CFR were extremely low. Besides, CER and CREA had a higher concentration of synephrine than CFR. The phytochemicals of CER, CREA, and CFR were significantly different, which might provide chemical evidence for the comparative pharmacological activities’ research and rational application of them.
Comparative analysis of the chemical constituents in Citri Exocarpium Rubrum, Citri Reticulatae Endocarpium Alba, and Citri Fructus Retinervus.
Two essential oils were isolated from discarded perfume lemon and leaves (
Citrus limon
(L.) Burm. F.) by hydro-distillation with good yield (0.044% for perfume lemon and 0.338% for leaves). Their ...biological activities were evaluated against five selected bacterial strains and
Aedes albopictus
(
Ae. albopictus
, Diptera: Culicidae). Chemical composition indicated that both essential oils were rich in essential phytochemicals including hydrocarbons, monoterpenes and sesquiterpene. These constituents revealed some variability among the oils displaying interesting chemotypes (
R
)-(+)-limonene (12.29–49.63%), citronellal (5.37–78.70%) and citronellol (2.98–7.18%). The biological assessments proved that the two essential oils had similar effect against bacterial (inhibition zones diameter ranging from 7.27 ± 0.06 to 10.37 ± 0.15 mm; MICs and MBCs ranging from 1.6 to 6.4 mg/mL); against
Ae. albopictus
larvae (LC
50
ranging from 384.81 to 395.09 ppm) and adult mosquito (LD
50
ranging from 133.059 to 218.962 μg/cm
2
); the activity of the two chemotypes ((
R
)-(+)-limonene and citronellal): larvae (LC
50
ranging from 267.08 to 295.28 ppm), which were all presented in dose-dependent manners. Through this work, we have showcased that recycling and reusing of agriculture by-products, such as discarded perfume lemon and leaves can produce eco-friendly alternatives in bacterial disinfectants and mosquito control product.
A rapid and simple method based on the coupling of supercritical fluid extraction and ultra-high-performance liquid chromatography combined with Q-Exactive Orbitrap tandem mass spectrometry ...(SFE-UHPLC-Q-Exactive Orbitrap-MS) detection for the identification of compounds from Citrus reticulata semen (CRS) was developed for the first time in this study. Through the optimization of the SFE parameters including extractive pressure, extractive temperature, and time, most of the compounds were successfully extracted at 50 °C, 33 MPa, and 2 h without an entraining agent, among which 32 compounds were successfully identified. Moreover, the operating conditions of UHPLC-Q-Exactive Orbitrap-MS were also optimized for the analysis of the SFE extracts, and the extracts in the CRS showed good separation performance in 20 min. A total of 28 compounds from the SFE extract were identified by comparing the standard sample together with full scan and related literature data, among which esters and flavonoids were the major compounds identified in the CRS extracts. In addition, 2 phenols, 2 aldehydes, 2 triterpenes, and 5 other compounds were identified. The SFE-UHPLC-Q-Exactive Orbitrap-MS method was successfully validated and applied for the identification of compounds from the CRS.
As a commonly used hydraulic control component, the nozzle-flapper servo valve has the advantages of small structure and sensitive dynamic response. Due to the small size and complex structure of the ...servo valve, the classical control methods based on various feedback information cannot effectively improve its performance. In order to effectively improve the performance of the servo valve, this paper implemented the feedforward control method to suppress the dynamic performance of the armature assembly of the servo valve. The distributed parameters mathematical models of the armature assembly are established and verified which can accurately predict the vibration of the armature assembly. With the mathematical model, the feedforward control signal is calculated based on the real-time velocity, acceleration, and deflection of the armature assembly, respectively. The applicability of different signals is analyzed and the signals calculated by velocity and deflection are verified which can effectively suppress the vibration amplitude of armature assembly. The numerical simulation and experimental methods are implemented to verify the performance of different control signals and the optimal feedforward control signal under different external loads is obtained.
Citrus peel polymethoxyflavonoids (PMFs), which exhibited a significant anti-lipase effect in functional foods, were widely used in diet therapy. However, its biological application was limited by ...the low content in citrus peel and poor solubility in water. To enhance the solubility and anti-lipase activity of citrus peel PMFs, the citrus peel PMFs extract was enriched by reflux extraction. Then, liposomes loaded with citrus peel PMFs extract (namely, PLS) were prepared by thin film hydration-high pressure homogenization method. Citrus peel PMFs extract, mainly including eight PMFs compounds such as nobiletin and tangeretin, were enriched from citrus peel with purity of 82.76%. The PLS prepared were spherical vesicles under transmission electron microscope (TEM), and they exhibited small particle size (70.94 ± 0.82 nm), high encapsulation efficiency (87.59 ± 2.55%), sustained release and good stability in characteristics. Furthermore, PLS showed significantly better solubility and stronger anti-lipase effect compared with free PMFs. Overall, PLS might be a potential anti-lipase inhibitor for obesity, which might allow the effective valorization of citrus peel and provide a potential value-added product used in dietary supplement.
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•PMFs (polymethoxyflavonoids) extracts and PLS (PMFs-loaded liposomes) were prepared.•Eight PMFs components were obtained and the purity of PMFs was 82.76%.•PLS exhibited good characteristics after formulation optimization.•PLS showed better solubility and stronger anti-lipase effect compared with free PMFs.•PLS might be a potential active ingredient for obesity management.
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