Human herpes virus 6A (HHV-6A) is able to integrate into the telomeric and subtelomeric regions of human chromosomes representing chromosomally integrated HHV-6A (ciHHV-6A). The integration starts ...from the right direct repeat (DR
) region. It has been shown experimentally that perfect telomeric repeats (pTMR) in the DR
region are required for the integration, while the absence of the imperfect telomeric repeats (impTMR) only slightly reduces the frequency of HHV-6 integration cases. The aim of this study was to determine whether telomeric repeats within DR
may define the chromosome into which the HHV-6A integrates. We analysed 66 HHV-6A genomes obtained from public databases. Insertion and deletion patterns of DR
regions were examined. We also compared TMR within the herpes virus DR
and human chromosome sequences retrieved from the Telomere-to-Telomere consortium. Our results show that telomeric repeats in DR
in circulating and ciHHV-6A have an affinity for all human chromosomes studied and thus do not define a chromosome for integration.
Bulk oriented films based on ultrahigh molecular weight polyethylene (UHMWPE) with a drawing ratio of 35 were prepared by using a low solvent concentration. Bulk oriented films were filled with ...fluorinated multi-walled carbon nanotubes (FMWCNTs). The structure of bulk oriented films on UHMWPE, which were manufactured at different stages of orientation, was investigated by scanning electron microscope (SEM) and differential scanning calorimetry (DSC). The addition of FMWCNTs at a concentration of 0.05 wt % in bulk oriented UHMWPE films led to an increase in the tensile strength by 10% (up to 1020 ± 23 MPa) compared to unfilled oriented films. However, the addition of FMWCNTs at a concentration of more than 0.5 wt % led to a decrease in tensile strength due to excessive accumulation of nanotubes and hindering of self-diffusion of UHMWPE macromolecules. The multiple increase in tensile strength, doubling the hardness, the formation of fibrillar structure, and the presence of carbon nanotubes led to a significant increase in tribological properties in bulk oriented films. Bulk oriented UHMWPE/1% FMWCNT films can be operated at a maximum contact pressure that is 18 times higher and exhibit a specific wear rate more than an order of magnitude and less than the traditional UHMWPE of isotropic structure. Bulk oriented UHMWPE/1% FMWCNT films have an extremely low dry coefficient of friction (COF) of 0.075 at a contact pressure of 31 MPa. The developed bulk oriented films can be used for manufacturing frictional surfaces for sliding bearings, or for acetabular cups for knee and hip endoprostheses.
Bacterial microbiota in stool may vary over a wide range, depending on age, nutrition, etc. The purpose of our work was to discriminate phyla and genera of intestinal bacteria and their biodiversity ...within a healthy population (North-Western Russia) compared to the patients with type 1 diabetes mellitus (T1DM). The study group included 183 healthy persons 2 to 53 years old (a mean of 26.5±1.0 years old), and 41 T1DM patients (mean age 18.2±1.8 years old). The disease onset was at 11±1.5 years, with a T1DM experience of 7±1.5 years. Total DNA was isolated from the stool samples, and sequencing libraries were prepared by amplifying the V3-V4 region of the 16S rRNA gene sequenced by Illumina MiSeq. Bioinformatic processing of NGS databases was adapted for microbiota evalutaion. Despite the broad scatter, the biological diversity for bacterial microbiota expressed as the Shannon index was significantly increased from younger to older ages in the comparison group, higher in adult healthy persons, with a trend for decrease in the
phylum which includes
species. Similar but non-significant age trends were noted in the T1DM group. Concordant with the
prevalence in stool samples of diabetic patients, some anaerobic bacteria (
,
and
,
) were enriched in the T1DM microbiome against controls. Hence, correction of microbiota for
and
requires future search for new probiotics. Lower abundance of
and
in T1DM suggests potential usage of Bifidobacter-based probiotics in this cohort.
In the Republic of Sakha (Yakutia), from 2001 to 2020, the incidence of asthma is increasing from 4.1 per 1000 population to 12.5 per 1000 population. Considering that currently one of the most ...effective modern methods of treating severe asthma is genetic engineering biological therapy, research on biologically active medications, as well as the lack of research on this problem in the Yakut population, our work seems relevant. The purpose of this study was to evaluate the effectiveness of treatment with genetic engineering biological drugs (GEBD) in patients with severe asthma in the Yakut population. The study included 17 patients suffering from severe asthma. In 5 patients with severe asthma, the effectiveness of the following GEBD was assessed: anti IL-4, IL-13 drug, in 6 patients – anti IL-5 drug, in 6 patients – anti IgE drug. The effectiveness of treatment was assessed using the ACQ-5 symptom control test, the number of exacerbations over the previous 12 months of asthma therapy, IgE levels, the absolute number of eosinophils, indicators of external respiratory function, and the need of patients for additional asthma therapy. Before initiation, 8 (47.10%) patients had exacerbations and sought medical help; 2 patients were hospitalized in the pulmonology department; after 6 and 12 months, no exacerbations or hospitalizations in specialized hospitals were registered. When using a GEBD for 12 months, the need for inhaled corticosteroids decreased in micrograms/day. Thus, treatment of severe asthma in the Yakut population with GEBD makes it possible to achieve control over its symptoms, reduce the risk of severe exacerbations and the number of hospitalizations, and prevent side effects from high doses of standard therapy.
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The possibility of a change in supramolecular structure of ultra high molecular weight polyethylene and its influence on the polymer mechanical and tribological properties has been ...demonstrated.
GaMnAs layers fabricated on GaAs(001) substrates by laser ablation technique were studied using the magneto-optical transversal Kerr effect (TKE) (E = 0.5 - 4.0 eV) and spectral ellipsometry (E = 1.4 ...- 4.5 eV). Obtained spectral, temperature and magnetic field TKE dependences shown that the used technique allowed us to obtain ferromagnetic (Ga,Mn)As layers, whose composition was close to single-phase one (without MnAs inclusions). Spectral dependences of the off-diagonal components (e’ = e’1 - ie’2) of the permittivity tensor and also of the magnetic circular dichroism (MCD) were calculated for ferromagnetic (Ga,Mn)As sample. The calculated dependences were compared with the published magneto-optical spectra of (Ga,Mn)As layers grown by molecular beam epitaxy.
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