Intracellular trafficking is an important event in the control of type and number of ion channels expressed on the cell surface. In this study, we have identified molecular domains involved in ...assembly and trafficking of the human small conductance Ca super(2) super(+)-activated K super(+) channel SK3. Deletion of the N-terminus, the C-terminus, or the calmodulin-binding domain (CaMBD) led to retention of SK3 channels in the endoplasmic reticulum. Presence of the CaMBD allowed trafficking to the Golgi complex, and sequences downstream were required for efficient transport to the plasma membrane, suggesting several steps in the control of SK3 forward trafficking. Co-immunoprecipitation studies demonstrated that SK3 subunits lacking the N-terminus, the CaMBD, or the distal C-terminus, but not the entire C-terminus, were able to oligomerize with wild-type SK3 subunits. Thus, these two C-terminal regions of SK3 seem to contribute to assembly and trafficking of channels whereas the N-terminus is necessary for trafficking but not sufficient for oligomerization.
This article aims to explore how intimacy is constructed and experienced among couples formed through transnational and arranged marriages, between Morocco and Italy. The analysis, conducted with a ...qualitative approach, begins by retracing the process of couple formation and the role that kinship plays in facilitating this transition in migrants' life courses. I then consider how love and intimacy are intertwined with the wider effort of family settlement in the Italian context. The investigation is developed in dialogue with the literature on the detraditionalization of intimacy, on one side, and transnational marriages, on the other. I argue that these couples achieve intimacy by pursuing copious relationships resulting from their ability to navigate among family extension and nuclearization, independence and reciprocal commitment, kin expectations and self-determination.
The eye, designed to admire, can never really open wide enough.Curiously, while Marcel Duchamp judged eroticism to be a vital dynamic in his creation, his work has never really been viewed through ...that particular spy hole. The need to convene researchers from all over the world, each with their own lorgnette, spyglass or whatever, has made itself keenly felt. The challenge was to lift the veil - and these spectators eagerly accepted. They went through the work with a fine tooth comb (at the risk of coming away with no more than a few hairs), put their ideas to the test of the chocolate-grinder... and came up with reflections on DADA, Surrealism and its asides, the latest developments in art and life...More than enough to prove that Duchamp, Selavy...
The aim of this article is to consider how migration and fertility constitute interrelated events among Moroccan women in Italy. Through a qualitative approach, I analyse the evolution of their life ...courses, exploring how these women, with their partners, manage to have children in migration. This viewpoint is particularly relevant considering the Italian context, which has historically been characterized by low fertility rates. I argue that the interweaving of migration and fertility these women deploy between Morocco and Italy stems from a transnational mobilization of reproductive resources, namely an array of relations, practices and values that make marriage, childbearing and the raising of offspring sustainable and attractive in migration. Through this frame, I reconstruct the ways these women manage their fertility, distinguishing between expected and unexpected children. Finally, I describe the process through which they achieve multi-child families (4–6 children).
Eighty psychrotrophic bacterial strains, isolated from different northwest Italian bulk tank milks destined for Grana Padano cheese production, were identified by 16S rRNA gene amplification and ...partial sequence analysis of the rpoB gene. Pseudomonas spp. were the most commonly occurring contaminants, P. fluorescens being the predominant isolated species, along with Enterobacteriaceae, primarily Serratia marcescens. RAPD‐PCR was used to study genetic variability and distinguish closely related strains; a high degree of genetic heterogeneity among the strains was highlighted. All the strains were characterized for their ability to produce proteases, lipases and lecithinases at different temperatures (7, 22, and 30 °C). Forty‐one of the psychrotrophic strains were positive for all the enzymatic activities. The highest number of positive strains for all the incubation temperatures was found for lipolytic activity (59), followed by proteolytic (31) and lecithinase (28) activities, and the enzymatic traits varied among the Pseudomonas and Enterobacteriaceae strains. The proteolytic psychrotrophic strains were screened for the presence of the aprX gene, coding for a heat‐resistant metalloprotease in Pseudomonas spp. The aprX gene was detected in 19 of 63 Pseudomonas strains, and was widespread in the P. fluorescens strains (14/19).
Clinical manifestations of COVID-19 caused by the new coronavirus SARS-CoV-2 are associated with age
. Adults develop respiratory symptoms, which can progress to acute respiratory distress syndrome ...(ARDS) in the most severe form, while children are largely spared from respiratory illness but can develop a life-threatening multisystem inflammatory syndrome (MIS-C)
. Here, we show distinct antibody responses in children and adults after SARS-CoV-2 infection. Adult COVID-19 cohorts had anti-spike (S) IgG, IgM and IgA antibodies, as well as anti-nucleocapsid (N) IgG antibody, while children with and without MIS-C had reduced breadth of anti-SARS-CoV-2-specific antibodies, predominantly generating IgG antibodies specific for the S protein but not the N protein. Moreover, children with and without MIS-C had reduced neutralizing activity as compared to both adult COVID-19 cohorts, indicating a reduced protective serological response. These results suggest a distinct infection course and immune response in children independent of whether they develop MIS-C, with implications for developing age-targeted strategies for testing and protecting the population.
SARS-CoV-2 has caused a global pandemic of COVID-19 since its emergence in December 2019. The infection causes a severe acute respiratory syndrome and may also spread to central nervous system ...leading to neurological sequelae. We have developed and characterized two new organotypic cultures from hamster brainstem and lung tissues that offer a unique opportunity to study the early steps of viral infection and screening antivirals. These models are not dedicated to investigate how the virus reaches the brain. However, they allow validating the early tropism of the virus in the lungs and demonstrating that SARS-CoV-2 could infect the brainstem and the cerebellum, mainly by targeting granular neurons. Viral infection induces specific interferon and innate immune responses with patterns specific to each organ, along with cell death by apoptosis, necroptosis, and pyroptosis. Overall, our data illustrate the potential of rapid modeling of complex tissue-level interactions during infection by a newly emerged virus.
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