Infectious diseases are a leading threat to public health. Accurate and timely monitoring of disease risk and progress can reduce their impact. Mentioning a disease in social networks is correlated ...with physician visits by patients, and can be used to estimate disease activity. Dengue is the fastest growing mosquito-borne viral disease, with an estimated annual incidence of 390 million infections, of which 96 million manifest clinically. Dengue burden is likely to increase in the future owing to trends toward increased urbanization, scarce water supplies and, possibly, environmental change. The epidemiological dynamic of Dengue is complex and difficult to predict, partly due to costly and slow surveillance systems.
In this study, we aimed to quantitatively assess the usefulness of data acquired by Twitter for the early detection and monitoring of Dengue epidemics, both at country and city level at a weekly basis. Here, we evaluated and demonstrated the potential of tweets modeling for Dengue estimation and forecast, in comparison with other available web-based data, Google Trends and Wikipedia access logs. Also, we studied the factors that might influence the goodness-of-fit of the model. We built a simple model based on tweets that was able to 'nowcast', i.e. estimate disease numbers in the same week, but also 'forecast' disease in future weeks. At the country level, tweets are strongly associated with Dengue cases, and can estimate present and future Dengue cases until 8 weeks in advance. At city level, tweets are also useful for estimating Dengue activity. Our model can be applied successfully to small and less developed cities, suggesting a robust construction, even though it may be influenced by the incidence of the disease, the activity of Twitter locally, and social factors, including human development index and internet access.
Tweets association with Dengue cases is valuable to assist traditional Dengue surveillance at real-time and low-cost. Tweets are able to successfully nowcast, i.e. estimate Dengue in the present week, but also forecast, i.e. predict Dengue at until 8 weeks in the future, both at country and city level with high estimation capacity.
Aedes aegypti is an important vector for arboviroses and widely distributed throughout the world. Climatic factors can influence vector population dynamics and, consequently, disease transmission. ...The aim of this study was to characterize the temporal dynamics of an Ae. aegypti population and dengue cases and to investigate the relationship between meteorological variables and mosquito infestation.
We monitored and analyzed the adult female Ae. aegypti population, the dengue-fever vector, in Porto Alegre, a subtropical city in Brazil using the MI-Dengue system (intelligent dengue monitoring). This system uses sticky traps to monitor weekly infestation indices. We fitted generalized additive models (GAM) with climate variables including precipitation, temperature and humidity, and a GAM that additionally included mosquito abundance in the previous week as an explanatory variable. Logistic regression was used to evaluate the effect of adult mosquito infestation on the probability of dengue occurrence.
Adult mosquito abundance was strongly seasonal, with low infestation indices during the winters and high infestation during the summers. Weekly minimum temperatures above 18 °C were strongly associated with increased mosquito abundance, whereas humidity above 75% had a negative effect on abundance. The GAM model that included adult mosquito infestation in the previous week adjusted and predicted the observed data much better than the model which included only meteorological predictor variables. Dengue was also seasonal and 98% of all cases occurred at times of high adult Ae. aegypti infestation. The probability of dengue occurrence increased by 25%, when the mean number of adult mosquitos caught by monitoring traps increased by 0.1 mosquitoes per week.
The results suggest that continuous monitoring of dengue vector population allows for more reliable predictions of infestation indices. The adult mosquito infestation index was a good predictor of dengue occurrence. Weekly adult dengue vector monitoring is a helpful dengue control strategy in subtropical Brazilian cities.
In the past decade fish farming has become an important economic activity in the Occidental Brazilian Amazon, where the number of new fish farms is rapidly increasing. One of the primary concerns ...with this phenomenon is the contribution of fishponds to the maintenance and increase of the anopheline mosquito population, and the subsequent increase in human malaria burden. This study reports the results of a 2-year anopheline abundance survey in fishponds and natural water bodies in a malaria-endemic area in northwest Brazil. The objective of this study was to investigate the contribution of natural water bodies (rivers, streams, creeks, ponds, and puddles) and artificial fishponds as breeding sites for Anopheles spp. in Mâncio Lima, Acre and to investigate the effect of limnological and environmental variables on Anopheles spp. larval abundance.
Natural water bodies and fishponds were sampled at eight different times over 2 years (early, mid and late rainy season, dry season) in the Amazonian town of Mâncio Lima, Acre. Anopheline larvae were collected with an entomological dipper, and physical, chemical and ecological characteristics of each water body were measured. Management practices of fishpond owners were ascertained with a systematic questionnaire.
Fishponds were four times more infested with anopheline larvae than natural water bodies. Electrical conductivity and the distance to the nearest house were both significant inverse predictors of larval abundance in natural water bodies. The density of larvae in fishponds raised with increasing border vegetation. Fishponds owned by different farmers varied in the extent of anopheline larval infestation but ponds owned by the same individual had similar infestation patterns over time. Commercial fishponds were 1.7-times more infested with anopheline larvae compared to fishponds for family use.
These results suggest that fishponds are important breeding sites for anopheline larvae, and that adequate management activities, such as removal of border vegetation could reduce the abundance of mosquito larvae, most importantly Anopheles darlingi.
A longitudinal study was conducted in Manaus, Brazil, to monitor changes of adult Aedes aegypti (L.) abundance. The objectives were to compare mosquito collections of two trap types, to characterise ...temporal changes of the mosquito population, to investigate the influence of meteorological variables on mosquito collections and to analyse the association between mosquito collections and dengue incidence. Mosquito monitoring was performed fortnightly using MosquiTRAPs (MQT) and BG-Sentinel (BGS) traps between December 2008-June 2010. The two traps revealed opposing temporal infestation patterns, with highest mosquito collections of MQTs during the dry season and highest collections of BGS during the rainy seasons. Several meteorological variables were significant predictors of mosquito collections in the BGS. The best predictor was the relative humidity, lagged two weeks (in a positive relationship). For MQT, only the number of rainy days in the previous week was significant (in a negative relationship). The correlation between monthly dengue incidence and mosquito abundance in BGS and MQT was moderately positive and negative, respectively. Catches of BGS traps reflected better the dynamic of dengue incidence. The findings help to understand the effects of meteorological variables on mosquito infestation indices of two different traps for adult dengue vectors in Manaus.
Carbon dioxide (CO2) is an important component for activating and attracting host-seeking mosquitoes. The BG-Sentinel® trap is a well-established monitoring tool for capturing Culicidae, but CO2 role ...for the trap effectiveness has not been evaluated in highly urbanised areas. The objective was to evaluate the effectiveness of BG-Sentinel traps baited with and without CO2 for capturing urban mosquitoes. Fifteen areas were selected within the city of Manaus, Brazil, where four BG-Sentinels were operated for 24 h, two of them with CO2 and two without CO2. Captured Aedes aegypti females were dissected for the determination of their parity status. A significantly higher proportion of traps (from 32-79%) were positive for female Ae. aegypti when using the BG-Sentinel with CO2 (χ2 = 11.0271, p ≤ 0.001). Catches of female Culex spp were six times higher in CO2 traps (Mann-Whitney U test = 190.5; p = 0.001). Parity rates were similar for both traps. This study showed that CO2 has primarily an enhancing effect on the efficacy of BG-Sentinel for capturing Culex spp in Manaus. For Ae. aegypti, the positivity rate of the trap was increased, when CO2 was added.
The objective of this study was to evaluate the effectiveness of Aedes aegypti mass trapping using the sticky trap MosquiTRAP (MQT) by performing a cluster randomised controlled trial in Manaus, ...state of Amazonas, Brazil. After an initial questionnaire and baseline monitoring of adult Ae. aegypti abundance with BG-Sentinel (BGS) traps in six clusters, three clusters were randomly assigned to the intervention arm where each participating household received three MQTs for mass trapping during 17 months. The remaining three clusters (control arm) did not receive traps. The effect of mass trapping on adult Ae. aegypti abundance was monitored fortnightly with BGS traps. During the last two months of the study, a serological survey was conducted. After the study, a second questionnaire was applied in the intervention arm. Entomological monitoring indicated that MQT mass trapping did not reduce adult Ae. aegypti abundance. The serological survey indicated that recent dengue infections were equally frequent in the intervention and the control arm. Most participants responded positively to questions concerning user satisfaction. According to the results, there is no evidence that mass trapping with MQTs can be used as a part of dengue control programs. The use of this sticky trap is only recommendable for dengue vector monitoring.
In the first three chapters of the present thesis, the BG-Sentinel (BGS), the MosquiTRAP (MQT) and the BG-Mosquitito (BGM) mosquito traps, designed for Aedes aegypti (L.) monitoring, were evaluated ...for their use as dengue vector control tools in mass trapping experiments. In the fourth chapter, longitudinal monitoring data of BGSs and MQTs were compared and associations between trap catches, dengue incidence and meteorological variables were analyzed. The first experiment evaluated the effect of mass trapping with BGSs on adult populations of dengue vectors in Manaus (AM). Results of entomological monitoring indicate that mass trapping significantly reduced the abundance of adult females Ae. aegypti during the first five rainy months of the study, but not during the dry season. In the subsequent rainy season, less females were caught in the treated areas, however without significant difference in comparison to the control arm. The serological study for the presence of DENV specific IgM antibodies indicated, that recent dengue infections were less frequent in the mass trapping arm, but the difference in comparison to the control arm was not significant. The results were not completely conclusive but there is evidence that the BGS is a promising tool that might be used as a part of dengue control programs. However, additional studies are necessary to prove a significant effect of BGS mass trapping on vector abundance and dengue virus transmission. In the second chapter, MQTs were evaluated as a dengue vector control tool in Manaus. Significantly more female Ae. aegypti were present in the treated areas during the intervention, in comparison to the untreated control areas. Therefore, entomological monitoring suggested that MQT mass trapping did not reduce the abundance of adult dengue vectors. The frequency of recent dengue infections was not reduced in the mass trapping arm. According to the results of this study there is no evidence that MQT mass trapping (using three traps per house) might be used as a part of dengue control programs in Manaus. Therefore, the use of MQT is only recommendable for Ae. aegypti monitoring. In the third experiment, mass trapping with BGMs was used in a neighborhood of Sete Lagoas (MG) with high infestation of Ae. aegypti, according to the Intelligent Dengue Monitoring (MI-D). Monitoring was performed using MQTs e BGMs. Results of MQT monitoring revealed that there were significantly less gravid Ae. aegypti in the mass trapping area. The BGM monitoring traps however suggested no significant difference between the adult female dengue vector catches in the two areas. The results indicate, that BGMs might be used as a part of dengue control strategies in combination with MI-D. Additional studies are necessary to prove a significant effect of the traps. The objectives of the study described in chapter IV were to compare mosquito collections of two trap types, to characterize temporal changes of the mosquito population and to investigate the influence of meteorological variables on mosquito collections. Additionally, associations between adult mosquito collections and dengue incidence were analyzed. Correlation between mean monthly Ae. aegypti collections and monthly dengue incidence was moderate negative for MQT and moderate positive for BGS. The two traps revealed differing temporal infestation patterns, with highest mosquito collections of MQTs during the dry season and highest collections of BGSs during the first rainy season. Both traps were sensitive to detect the presence of dengue vectors in all monitoring weeks. Several meteorological variables were significant predictors of mosquito collections in BGS, but for MQT, only the number of rainy days in the previous week was significant. The findings help to understand the effects of meteorological variables on mosquito infestation indices of two different traps for adult dengue vectors in the climatic conditions of Manaus.
Nos primeiros três capítulos do presente trabalho, as armadilhas BG-Sentinel (BGS), MosquiTRAP (MQT) e BG-Mosquitito (BGM), projetadas para o monitoramento de Aedes aegypti (L.), foram avaliadas para o seu uso no controle de vetores da dengue em experimentos de coleta massal. No quarto capítulo, dados longitudinais de monitoramento com BGSs e MQTs foram comparados e as associações entre as capturas, a incidência da dengue e as variáveis meteorológicas foram analisadas. No primeiro experimento, o efeito da coleta massal com BGSs sobre as populações adultas de vetores da dengue em Manaus (AM) foi avaliado. Os resultados do monitoramento entomológico indicaram que a coleta massal reduziu significativamente a abundância de Ae. aegypti fêmeas, nos primeiros cinco meses do estudo (estação chuvosa) mas não na época de seca. Nos meses chuvosos subsequentes, menos fêmeas foram capturadas nas áreas tratadas porém, não houve diferença significativa em relação às áreas sem coleta massal. A pesquisa de anticorpos específicos (IgM) indicou que infecções recentes de DENV foram menos frequentes nas áreas tratadas, mas a diferença em relação às de controle não foi significativa. Os resultados não foram conclusivos mas, há evidências de que a BGS é uma ferramenta promissora, que pode ser utilizada como um componente de programas de controle da dengue. Estudos adicionais são necessários para comprovação de um efeito significativo da coleta massal sobre a abundância do vetor e a transmissão da dengue. No Capítulo II, o uso de MQTs foi avaliado para o controle de Ae. aegypti em Manaus. Durante a intervenção, houve um número significativamente maior de Ae. aegypti fêmeas nas áreas tratadas, em comparação com as de controle. Portanto, os resultados do monitoramento entomológico indicaram que a coleta massal com MQTs não reduziu a abundância fêmeas adultas de Ae. aegypti. A frequência de infecções recentes de DENV também não foi menor nas áreas de tratadas. A partir dos resultados obtidos, não houve evidências de que a coleta massal com MQT (três unidades por casa) poderia ser utilizada no combate dos vetores da dengue em Manaus, porém seu uso é recomendável apenas para o monitoramento de Ae. aegypti. No terceiro experimento, a coleta massal com BGMs foi utilizada em um bairro com infestação elevada em Sete Lagoas (MG), detectada pelo sistema MI-Dengue (MI-D). O monitoramento foi realizado com MQTs e BGMs. Os resultados do monitoramento com MQTs mostraram que, durante o período de intervenção, houve um número significativamente menor de Ae. aegypti fêmeas, na área de coleta massal. Contudo, não houve diferença significativa entre as capturas de Ae. aegypti fêmeas, em BGMs de monitoramento. Os resultados sugerem que as BGMs podem ser utilizadas como parte de uma estratégia de controle da dengue, juntamente com o MI-D. Entretanto, novos estudos são necessários para comprovação de um efeito significativo das armadilhas e para identificação da forma mais eficiente de combinação do MI-D com a coleta massal. O objetivo do experimento descrito no capítulo IV foi comparar as capturas de mosquitos de dois tipos de armadilhas para Ae. aegypti adultos, para caracterizar as alterações temporais da população e investigar a influência de variáveis meteorológicas sobre estas. Além disso, a associação entre a captura de mosquitos adultos e a incidência de dengue foi analisada. A correlação entre a média mensal de capturas de Ae. aegypti e a incidência mensal de dengue foi moderadamente negativa para MQTs e moderadamente positiva para BGSs. Os dois tipos de armadilhas revelaram diferentes padrões temporais de infestação, com capturas maiores da MQT na estação seca, e maiores da BGS na estação chuvosa. Ambas as armadilhas foram sensíveis para detecção de vetores da dengue, em todas as semanas de monitoramento. Inúmeras variáveis meteorológicas foram apontadas como preditores significativos para a captura de mosquitos em BGSs. Para a MQT, apenas o número de dias chuvosos na semana anterior, que apresentou uma associação negativa, foi significativo. Estes resultados contribuem na compreensão dos efeitos das variáveis meteorológicas sobre os índices de infestação de mosquitos de duas armadilhas diferentes para vetores da dengue adultos, nas condições climáticas de Manaus.
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