K17, an indigenous and heterotrophic nitrifying-aerobic denitrifying bacterium, was isolated from the soil of a weathered crust elution-deposited rare earth ore leaching site in Longnan County, ...China. Strain K17 was identified as
Pseudomonas mosselii
. In this study, the morphological characteristics of strain K17 were observed and the optimal ammonia nitrogen removal conditions for the strain were studied using a single-factor experiment. Key enzyme activities were determined, and we also explored the ammonia nitrogen removal process of strain K17 on simulated leaching liquor of the rare earth element leaching site. Based on the determination of ammonia nitrogen removal and enzyme activity, it was found that strain K17 has both heterotrophic nitrifying and aerobic denitrifying activities. In addition, single-factor experiments revealed that the most appropriate carbon source for strain K17 was sodium citrate with a C/N ratio of 10 and an initial NH
4
+
-N concentration of 100 mg/l. Furthermore, the optimal initial pH and rotation speed were 7 and 165 r/min, respectively. Under optimal conditions, the ammonia nitrogen removal efficiency of strain K17 was greater than 95%. As an indigenous bacterium, strain K17 has great potential for treating residual ammonium leaching solutions from rare earth element leaching sites.
Autophagy, a highly conserved process in eukaryotes that involves vacuolar degradation of intracellular components and decomposition of damaged or toxic constituents, is induced by endogenous ...reactive oxygen species (ROS) accumulation, endoplasmic reticulum stress, and other factors. In plants, the role of autophagy in the induction of programmed cell death (PCD) is still unclear. Here, we show that ROS contribute to the regulation of PCD during waterlogging (which results in oxygen depletion) via autophagy. In wild-type roots, waterlogging induces the transcription of hypoxia-responsive genes and respiratory burst oxidase homolog (RBOH)-mediated ROS production. It also altered the transcription level of alternative oxidase1a and the activity level of antioxidant enzymes. Moreover, waterlogging increased the transcription levels of autophagy-related (
) genes and the number of autophagosomes. Autophagy first occurred in the root stele, and then autophagosomes appeared at other locations in the root. In
mutants, upregulation of autophagosomes was less pronounced than in the wild type upon waterlogging. However, the accumulation of ROS and the level of cell death in the roots of
mutants were higher than those in the wild type after waterlogging. In conclusion, our results suggest that autophagy induced in
roots during waterlogging has an attenuating effect on PCD in the roots.
Because of its low whiteness, complex composition, radioactivity and high impurity percentage, the usage of phosphogypsum (PG) resources is limited. A theoretical foundation for upgrading and ...bleaching PG can be obtained by researching the presence and status of impurities in the material and its symbiotic connection with gypsum. This paper makes use of an automatic mineral phase analyzer, optical microscope, XRF, XRD and SEM-EDS. After analyzing the chemical makeup of PG, phase composition and particle size composition, the distribution law and symbiotic interaction between impurities and gypsum in various particle sizes were discovered. Using a flotation test, the process mineralogy analysis results were confirmed. According to the XRF and XRD study results, the primary impurity elements in PG are Si, P and F. Si is more abundant in PG that is between +850 μm and −37.5 μm in size. The concentrations of gypsum and quartz in PG are 82.59% and 8.73%, respectively, according to the results of XRD and process mineralogy. The monomer dissociation degree of the gypsum mineral phase is as high as 90.47%. Gibbsite and pyrite are the primary causes of the low whiteness of PG and are clearly related to the quartz mineral phase. The coupling process of “flotation + pickling” produced purified PG with a purity of 95.35%, whiteness of 70.76% and SiO2 content of 2.73%. The quality met the first-class index standards of PG in GB/T23456-2018.
A series of phenolic compounds containing 2-phenylphenol (PPE), bisphenol A (BPA), 4-isopropylphenol (IPE), 4-methylphenol (ME) and phenol (PE) were selected to investigate their major influence ...factors for their adsorption on graphene oxide (GO) and reduced graphene oxide (RGO) by studying their adsorption isotherms and kinetics. It was found that the adsorption of all tested phenols fitted well with the Freundlich model. In comparison, the adsorption ability of RGO with a stronger π-π interaction was superior to GO, which was confirmed by using naphthalene probe measurements. The thermodynamic characteristics, by studying the effect of the adsorption temperatures (298, 313 and 333 K), demonstrated that the adsorption process was spontaneous, exothermic and entropy-decreasing. The chemical structures of the phenols also affected their adsorption on GO and RGO. It was found that the adsorption capacities of phenols were, in order, PE (0.271 mmol g−1 on GO and 0.483 mmol g−1 on RGO) < ME (0.356 and 0.841 mmol g−1) < IPE (0.454 and 1.117 mmol g−1) < BPA (0.4 and 1.56 mmol g−1) < PPE (0.7 and 2.054 mmol g−1), which depended on the π-electron density of the benzene ring by means of a density functional theory (DFT) calculation. Undoubtedly, the reduction of GO and an increase in π-electron density on the chemical structures of phenols facilitated the adsorption.
•Persimmon tannin shows significant hypocholesterolaemic properties in in vitro studies.•The uptake of 22-NBD-cholesterol can be inhibited by persimmon tannin intervention.•Persimmon tannin ...down-regulated the expression of NPC1L1 genes.•Persimmon tannin exhibited potential in promoting cellular cholesterol efflux.•The regulation of persimmon tannin on cholesterol homeostasis was LXRα independent.
Persimmon tannin (proanthocyanidins) has been proven to exert significant hypocholesterolaemic effects in a variety of models. However, the regulatory mechanisms of persimmon tannin on cholesterol metabolism have yet to be fully understood. The present study investigated the regulation of persimmon tannin on the expression of genes critical for cholesterol absorption and cholesterol efflux. Our results indicated that persimmon tannin reduced the uptake of 22-NBD-cholesterol and inhibited the accumulation of cholesterol significantly in HepG2 and Caco-2 cell lines. The mRNA and proteins were quantified using real time PCR and western blot, respectively. The expression of SREBP-2 gene, critical for cholesterol synthesis, and NPC1L1 involved in cholesterol absorption were significantly inhibited by persimmon tannin. Moreover, persimmon tannin showed potential in promoting cholesterol efflux through up-regulating mRNA and/or protein levels of ABCA1, ABCG1, SR-BI, CYP7A1 and ABCG5/G8. However, the regulation effects of persimmon tannin on cholesterol homeostasis in both cell lines were LXRα independent.
Core–shell ZIFs wrapped CuO hybrid materials (CuO@ZIF-67(Co)) were designed, synthesized, characterized, and employed as peroxymonosulfate (PMS) activators to degrade methylene blue (MB). It ...demonstrated outstanding catalytic activity on account of the unique structure and the synergistic effect between CuO cores and ZIF-67(Co) shells, resulting in complete degradation of MB (10 mg/L) in 1 min. Reactive oxygen species (ROSs) research showed that both SO4− and OH were responsible for the removal of MB. The synergistic activation mechanisms in the CuO@ZIF-67(Co)/PMS system were investigated, which mainly involved the effective electron transfer of CuO and ZIF-67(Co) for accelerating the cycle of CuII/CuI and CoIII/CoII. This study broadens the application of MOF-derived materials for wastewater treatment.
Endothelial cell (EC) plays critical roles in vascular physiological and pathological processes. With the development of high-throughput technologies, transcriptomics analysis of EC has increased ...dramatically and a large amount of informative data have been generated. The dynamic patterns of gene expression in ECs under various conditions were revealed. Unfortunately, due to the lack of bioinformatics infrastructures, reuse of these large-scale datasets is challenging for many scientists. Here, by systematic re-analyzing, integrating, and standardizing of 203 RNA sequencing samples from freshly isolated mouse ECs under 71 conditions, we constructed an integrated mouse EC gene expression omnibus (ECO). The ECO database enables one-click retrieval of endothelial expression profiles from different organs under different conditions including disease models, genetic modifications, and clinically relevant treatments
. The EC expression profiles are visualized with user-friendly bar-plots. It also provides a convenient search tool for co-expressed genes. ECO facilitates endothelial research with an integrated tool and resource for transcriptome analysis. The ECO database is freely available at https://heomics.shinyapps.io/ecodb/.
Currently, the primary method for leaching rare earth ores is through in situ leaching. This approach involves contact between clay minerals and liquids, which can lead to the potential swelling of ...clay minerals with water, triggering natural disasters such as landslides. The main purpose of this study is to select the suitable anti-swelling solution for Hunan Jianghua ionic rare earth ore. According to the ore composition analysis, 88 wt% of Hunan Jianghua ionic rare earth ore is composed of halloysite clay mineral. Therefore, halloysite clay mineral is used to investigate its anti-swelling behavior in order to provide a reference for future research on the selection of raw ore swelling inhibitors. In this study, the traditional leaching agent, MgSO4 solution, was used as the solvent along with two additional compounds, CH3COOK and KCl, which were prepared in different concentrations to form a new composite swelling inhibitor solution to observe their effect on the swelling rate of halloysite clay mineral. At the same time, the seepage velocity of halloysite clay mineral with different anti-swelling solutions is studied. The results indicate that the optimal concentration in the CH3COOK + MgSO4 solution system is 0.05 mol/dm3. At this concentration, the swelling rate is 5.129%, the inhibition rate is 20.08%, and the seepage velocity rate is 12.51 × 10−3 cm/min, respectively. In KCl + MgSO4 solution, the swelling rate is 4.868%, the inhibition rate is 24.15% and the seepage velocity rate is 13.23 × 10−3 cm/min at the concentration of 0.02 mol/dm3, which is the optimum concentration. In addition, FTIR and TG studies have further demonstrated the mechanism by which these two composite bulking inhibitors inhibit the swelling of halloysite clay mineral.
Vasculature plays critical roles in the pathogenesis and neurological repair of traumatic brain injury (TBI). However, how vascular endothelial cells respond to TBI at the molecular level has not ...been systematically reviewed. Here, by integrating three transcriptome datasets including whole cortex of mouse brain, FACS-sorted mouse brain endothelial cells, and single cell sequencing of mouse brain hippocampus, we revealed the key molecular alteration of endothelial cells characterized by increased Myc targets and Epithelial-Mesenchymal Transition signatures. In addition, immunofluorescence staining of patients' samples confirmed that IGFBP7 was up-regulated in vasculature in response to TBI. TGFβ1, mainly derived from microglia and endothelial cells, sufficiently induces IGFBP7 expression in cultured endothelial cells, and is significantly upregulated in response to TBI. Our results identified IGFBP7 as a potential biomarker of vasculature in response to TBI, and indicate that TGFβ signaling may contribute to the upregulation of IGFBP7 in the vasculature.
PDF
