Abstract
Polymers prepared by chemical vapor deposition (CVD) polymerization have found broad acceptance in research and industrial applications. However, their intrinsic lack of degradability has ...limited wider applicability in many areas, such as biomedical devices or regenerative medicine. Herein, we demonstrate, for the first time, a backbone‐degradable polymer directly synthesized via CVD. The CVD co‐polymerization of 2.2
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‐cyclophanes with cyclic ketene acetals, specifically 5,6‐benzo‐2‐methylene‐1,3‐dioxepane (BMDO), results in well‐defined, hydrolytically degradable polymers, as confirmed by FTIR spectroscopy and ellipsometry. The degradation kinetics are dependent on the ratio of ketene acetals to 2.2
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‐cyclophanes as well as the hydrophobicity of the films. These coatings address an unmet need in the biomedical polymer field, as they provide access to a wide range of reactive polymer coatings that combine interfacial multifunctionality with degradability.
Doppel‐„Klick“: Zwei verschiedene Moleküle können durch die unterschiedlichen Reaktivitäten aktivierter und nichtaktivierter Alkinyl‐Gruppen nacheinander auf definierten Bereichen der gleichen ...Oberfläche immobilisiert werden (siehe Bild). Die Reaktionen wurden bei Raumtemperatur in Wasser durchgeführt; der erste Immobilisierungsschritt benötigte keinen, der zweite hingegen einen Kupfer(I)‐Katalysator.
Polyethersulfone-Activated carbon (PES-AC) hybrid particles are prepared using a liquid-liquid phase separation technique. The PES-AC hybrid particles are then used for the removal of phenobarbital ...(PB) from its aqueous solutions. The adsorption ability of the particles increased significantly when activated carbon was embedded into the particles, and with the increase of the activated carbon content in the hybrid particles, the PB adsorbed amount increased. Also when the PB concentration increased, the adsorbed amount increased. The adsorption of PB to the hybrid particles belongs to the Freundlich adsorption. To more effectively remove PB, PES-AC particle columns were prepared; and the particle column showed more high removal efficiency for PB. The results suggested that the PES -AC hybrid particles have a potential to be used for the removal of PB, and may be used in blood purification.
Polyethersulfone (PES) dead-end tubes were fabricated by means of a phase inversion technique, and then were used as scaffolds for artificial lacrimal glands. The wall of the dead-end tubes could ...allow nutrients such as ascorbic acid, L-tryptophan, and glucose to pass through, but prevents rat IgG from passing through. Lacrimal acinar epithelial cells of Sprague-Dawley rats were cultured in vitro, and cell-associated secretory component was detected with an immunofluorescence technique to identify the acinar cells. The second passage of the cells showed high degree of cellular differentiation, and was used to seed on the PES tubes. The results showed that the PES tube could support the attachment, the growth, and the proliferation of the rat lacrimal acinar cells. Thus, PES is a substrate for the growth of lacrimal acinar cells and may be a useful scaffolding biomaterial for tissue engineering, such as a scaffold for artificial lacrimal glands.
This paper considers the steady Swift-Hohenberg equation u＇＇＇＋β2u＇＇＋u^3-u=0.Using the dynamic approach, the authors prove that it has a homoclinic solution for each β∈ （4√8-ε0,4 √8）, where ε0 is a ...small positive constant. This slightly complements Santra and Wei＇s result Santra, S. and Wei, J., Homoclinic solutions for fourth order traveling wave equations, SIAM J. Math. Anal., 41, 2009, 2038-2056, which stated that it admits a homoclinic solution for each β∈C （0,β0） where β0 = 0.9342 ...
Objective: High-dose chemotherapy（HDC） followed by autologous stem cell transplantation（ASCT） is the standard of care in the upfront or relapsed/refractory setting in some patients with non-Hodgkin ...lymphoma（NHL）.However,a proportion of patients do not respond to ASCT.18F-fluorodeoxyglucose（FDG） positron emission tomography（PET）/computed tomography（CT） has been widely used for staging,response evaluation,and prognosis prediction.Here,we investigated the prognostic role of PET/CT in NHL patients before and after ASCT.Methods: A retrospective study was conducted at Peking University Cancer Hospital.All NHL patients who underwent ASCT between March 2010 and July 2016 were identified.Patients who had PET/CT scan before and after ASCT were included.Deauville criteria（5-point scale） were used to interpret PET scans.Univariate and multivariate survival analyses were performed using Cox regression.The predictive value of PET scanning was estimated by comparing the area under the receiver operating characteristic（ROC） curve.Results: In total,79 patients were enrolled in this study.In univariate analysis,pre-and post-ASCT PET result was identified as prognostic factors for 3-year progression-free survival（PFS） and overall survival（OS）.Patients with negative pre-ASCT PET result demonstrated significantly better PFS（84.2% vs.54.2%） and OS（89.2% vs.63.6%） than patients with positive pre-ASCT PET result.PFS（91.6% vs.25.3%） and OS（96.5% vs.36.8%） were also significantly different between patients with negative and positive post-ASCT PET result.Multivariate analysis also showed a significant association between survival and post-ASCT PET result.ROC analysis revealed that the predictive value of post-ASCT PET result was superior to that of pre-ASCT PET result alone.Combined pre-and post-ASCT PET result is better for predicting outcomes in patients with NHL receiving transplantation.Deauville criteria score >3 was identified as the best cutoff value for post-ASCT PET.Conclusions: Post-ASCT PET result was more important than pre-ASCT PET result in predicting outcomes for NHL patients who underwent ASCT.The prognostic significance can be improved when combining preASCT PET result with post-ASCT PET result.Deauville criteria can be used for interpreting PET scans in this scenario.
Objective： Diffuse large B-cell lymphoma （DLBCL） is a heterogeneous disease. The prognostic factor currently used is not accurate enough to predict the outcomes of patients with DLBCL. The prognostic ...significance of interim PET/CT in DLBCL remains controversial. The aim of this study is to determine the predictive value of interim 18F-FDG PET/CT after first-line treatment in patients with DLBCL. Methods： Thirty-two patients with DLBCL underwent baseline, interim and post-treatment lSF-FDG PET/CT scans. Imaging results were analyzed for the survival of patients via software SPSS 13.0, retrospectively. Results： Thirty-one of the 32 patients were treated with R-CHOP regimen, and interim 18F-FDG PET/CT of 24 patients was performed after 2 cycles of treatment. After a median follow-up period of 16.7 months, the 2-year progression-free survival （PFS） rates were significantly different between the groups above and below SUVmax CUt-Off value of 2.5 （P=0.039）. No significant differences were found in the 2-year PFS rates if SUVm, cut-offvalues were set as 2.0 and 3.0, respectively （P=0.360; P=0.113）. Conclusions： Interim PET/CT could predict the prognosis of DLBCL patients with the SUVmax cut-off value of 2.5, but more clinical data should be concluded to confirm this conclusion.
Long non-coding RNAs (lncRNAs) represent a class of riboregulators that either directly act in long form or are processed into shorter microRNAs (miRNAs) and small interfering RNAs. Long noncoding ...RNAs (lncRNAs) are arbitrarily defined as RNA genes larger than 200 nt in length that have no apparent coding potential. lncRNAs have emerged as playing important roles in various biological regulatory processes and are expressed in a more tissue-specific manner than mRNA. Emerging evidence shows that lncRNAs participate in stress-responsive regulation.
In this study, in order to develop a comprehensive catalogue of lncRNAs in upland cotton under salt stress, we performed whole-transcriptome strand-specific RNA sequencing for three-leaf stage cotton seedlings treated with salt stress (S_NaCl) and controls (S_CK). In total we identified 1117 unique lncRNAs in this study and 44 differentially expressed RNAs were identified as potential non-coding RNAs. For the differentially expressed lncRNAs that were identified as intergenic lncRNAs (lincRNA), we analysed the gene ontology enrichment of cis targets and found that cis target protein-coding genes were mainly enriched in stress-related categories. Real-time quantitative PCR confirmed that all selected lincRNAs responsive to salt stress. We found lnc_388 was likely as regulator of Gh_A09G1182. And lnc_883 may participate in regulating tolerance to salt stress by modulating the expression of Gh_D03G0339 MS_channel. We then predicted the target mimics for miRNA in Gossypium. six miRNAs were identified, and the result of RT-qPCR with lncRNA and miRNA suggested that lnc_973 and lnc_253 may regulate the expression of ghr-miR399 and ghr-156e as a target mimic under salt stress.
We identified 44 lincRNAs that were differentially expressed under salt stress. These lincRNAs may target protein-coding genes via cis-acting regulation. We also discovered that specifically-expressed lincRNAs under salt stress may act as endogenous target mimics for conserved miRNAs. These findings extend the current view on lincRNAs as ubiquitous regulators under stress stress.
Superoxide dismutases (SODs) are a key antioxidant enzyme family, which have been implicated in protecting plants against the toxic effects of reactive oxygen species. Despite current studies have ...shown that the gene family are involved in plant growth and developmental processes and biotic and abiotic stress responses, little is known about its functional role in upland cotton.
In the present study, we comprehensively analyzed the characteristics of the SOD gene family in upland cotton (Gossypium hirsutum). Based on their conserved motifs, 18 GhSOD genes were identified and phylogenetically classified into five subgroups which corroborated their classifications based on gene-structure patterns and subcellular localizations. The GhSOD sequences were distributed at different densities across 12 of the 26 chromosomes. The conserved domains, gene family evolution cis-acting elements of promoter regions and miRNA-mediated posttranscriptional regulation were predicted and analyzed. In addition, the expression pattern of 18 GhSOD genes were tested in different tissues/organs and developmental stages, and different abiotic stresses and abscisic acid, which indicated that the SOD gene family possessed temporal and spatial specificity expression specificity and may play important roles in reactive oxygen species scavenging caused by various stresses in upland cotton.
This study describes the first genome-wide analysis of the upland cotton SOD gene family, and the results will help establish a foundation for the further cloning and functional verification of the GhSOD gene family during stress responses, leading to crop improvement.
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