To test the reliability and validity of the
(DEBQ) in an online format in university students from low-income regions.
We applied the questionnaire to a sample of 195 and 117 university students from ...a low-income region (Gini index of 0.56) to study validity and reliability, respectively. The DEBQ consists of 33 items on eating behavior in three dimensions/factors: emotional eating, restrained eating and external eating. The questionnaire was administered twice at 2-week intervals. We tested the reliability
temporal stability and internal consistency and construct validity
exploratory and confirmatory factor analysis.
For reliability, we identified an acceptable Spearman correlation coefficient (rho > 0.30 and
< 0.05) and Cronbach's alpha (
≥ 0.70) for all DEBQ items. In the exploratory analysis, we identified 6 factors representing a mix of original and additional factors, with an explained variance of 69.1%. In the confirmatory analysis with structural equation modeling, we observed better global model adjustment for the 6-factor model with the Tucker-Lewis index and comparative fit index closer to one, as well as root mean square error of approximation closer to zero than the original (3-factor) model. Using generalized structural equation modeling, we also observed a better fit in latent class modeling for the 6-factor model (AIC: 16990.67; BIC. 17874.38) than for the 3-factor model (AIC: 17904.09; BIC: 18342.67).
The online format of the DEBQ has acceptable reliability and validity for measuring eating behavior in university students from low-income regions.
Multinucleated giant cells (MGC) are characteristic cells in granulomatous disorders such as paracoccidioidomycosis (PCM) and also are formed in vitro from peripheral blood mononuclear cells by ...several stimuli. In this study, the authors investigated in vitro formation of MGC derived from monocytes of healthy individuals, stimulated with Paracoccidioides brasiliensis antigen (PbAg), compared with other stimuli such as IFN-γ and supernatant of Con-A-stimulated peripheral blood mononuclear cells (CM-ConA). Besides, the fungicidal activity of monocytes and monocyte-derived MGC challenged with P. brasiliensis were compared, at a ratio of one fungus per 50 monocytes. Results demonstrated that PbAg, IFN-γ, and CM-ConA stimuli were able to induce MGC generation, with fusion indices significantly higher than control cultures. Striking results were observed when MGC induced by PbAg and IFN-γ presented higher fungicidal activity than monocytes, submitted to the same stimuli, showing a better capacity of these cells to kill P. brasiliensis. In summary, the results suggest that PbAg is able to induce MGC generation, and these cells presented higher fungicidal activity against P. brasiliensis than monocytes.
Lipid transfer proteins (LTPs) were thus named because they facilitate the transfer of lipids between membranes in vitro. This study was triggered by the characterization of a 9‐kDa LTP from Capsicum ...annuum seeds that we call Ca‐LTP1. Ca‐LTP1 was repurified, and in the last chromatographic purification step, propanol was used as the solvent in place of acetonitrile to maintain the protein's biological activity. Bidimensional electrophoresis of the 9‐kDa band, which corresponds to the purified Ca‐LTP1, showed the presence of three isoforms with isoelectric points (pIs) of 6.0, 8.5 and 9.5. Circular dichroism (CD) analysis suggested a predominance of α‐helices, as expected for the structure of an LTP family member. LTPs immunorelated to Ca‐LTP1 from C. annuum were also detected by western blotting in exudates released from C. annuum seeds and also in other Capsicum species. The tissue and subcellular localization of Ca‐LTP1 indicated that it was mainly localized within dense vesicles. In addition, isolated Ca‐LTP1 exhibited antifungal activity against Colletotrichum lindemunthianum, and especially against Candida tropicalis, causing several morphological changes to the cells including the formation of pseudohyphae. Ca‐LTP1 also caused the yeast plasma membrane to be permeable to the dye SYTOX green, as verified by fluorescence microscopy. We also found that Ca‐LTP1 is able to inhibit mammalian α‐amylase activity in vitro.
This study aimed to detect and characterize antimicrobial proteins, especially antimicrobial peptides (AMPs) from leaves and roots of
Capsicum annuum
and to evaluate their inhibitory activities ...against different phytopathogenic fungi and the bacterium
Xanthomonas euvesicatoria
. Two methodologies were used for the extraction of peptides from leaves and roots of
C. annuum
: acid and ethanolic extraction. Extracts were subjected to reversed-phase chromatography on HPLC. The extraction and purification procedures were analysed by uni- and bi-dimensional electrophoresis in tricine gels. Our results show that alcoholic and acid extracts from both tissues can inhibit the growth of the phytopathogenics fungi
C. lindemuthianum
and
C. gloeosporioides
. The acid extracts from both tissues are active against
X. euvesicatoria
and only leaf extracts displayed specific inhibitory activity towards trypsin and α-amylase activity. The data compiled here aim to contribute to establish the multiplicity of potential uses of plant AMPs for the control of pests and pathogens of agricultural relevance.
Over the last several years, the activity of antimicrobial peptides (AMPs), isolated from plant species, against different microorganisms has been demonstrated. More recently, some of these AMPs have ...been described as potent inhibitors of α-amylases and serine proteinases from insects and mammals. The aim of this work was to obtain AMPs from protein extracts of a hybrid
Capsicum
(Ikeda × UENF 1381) seeds and to evaluate their microbial and enzyme inhibitory activities. Initially, proteins were extracted from the
Capsicum
hybrid seeds in buffer (sodium phosphate pH 5.4,) and precipitated with ammonium sulfate (90 % saturated). Extract of hybrid seeds was subjected to size exclusion chromatography, and three fractions were obtained: S1, S2 and S3. The amino acid sequence, obtained by mass spectrometry, of the 6 kDa peptide from the S3 fraction, named HyPep, showed 100 % identity with PSI-1.2, a serine protease inhibitor isolated from
C. annuum
seeds, however the bifunctionality of this inhibitor against two enzymes is being shown for the first time in this work. The S3 fraction showed the highest antifungal activity, inhibiting all the yeast strains tested, and it also exhibited inhibitory activity against human salivary and
Callosobruchus maculatus
α-amylases as well as serine proteinases.
Neste artigo discorremos acerca do círculo de leitura, uma forma de planejamento pensada para alunos do 6º ano do Ensino Fundamental. O livro, Como criar círculos de leitura na sala de aula, de ...Rildo Cosson (2021), revisita a obra anterior do autor Círculos de leitura e letramento literário, apresentando possibilidades de estratégias que auxiliem os alunos para a compreensão do texto literário, por meio de discussões coletivas, engajamento, reflexão comportamental, que poderão impactar na formação do leitor. À luz de teorias de alguns estudiosos que têm se proposto a abordar a questão da leitura e do sentido, apresentamos uma possibilidade de iniciar as crianças nesse processo, oportunizando que possam assumir o protagonismo dos debates e despertando a subjetividade do leitor.
Over the last several years, the activity of antimicrobial peptides (AMPs), isolated from plant species, against different microorganisms has been demonstrated. More recently, some of these AMPs have ...been described as potent inhibitors of alpha-amylases and serine proteinases from insects and mammals. The aim of this work was to obtain AMPs from protein extracts of a hybrid Capsicum (Ikeda x UENF 1381) seeds and to evaluate their microbial and enzyme inhibitory activities. Initially, proteins were extracted from the Capsicum hybrid seeds in buffer (sodium phosphate pH 5.4,) and precipitated with ammonium sulfate (90 % saturated). Extract of hybrid seeds was subjected to size exclusion chromatography, and three fractions were obtained: S1, S2 and S3. The amino acid sequence, obtained by mass spectrometry, of the 6 kDa peptide from the S3 fraction, named HyPep, showed 100 % identity with PSI-1.2, a serine protease inhibitor isolated from C. annuum seeds, however the bifunctionality of this inhibitor against two enzymes is being shown for the first time in this work. The S3 fraction showed the highest antifungal activity, inhibiting all the yeast strains tested, and it also exhibited inhibitory activity against human salivary and Callosobruchus maculatus alpha-amylases as well as serine proteinases.
Introdução: O Procedimento Operacional Padrão (POP) é um dos instrumentos administrativos essenciais às atividades da equipe de enfermagem que atua na assistência do Centro Cirúrgico (CC). A gestão ...do enfermeiro sobre a equipe cirúrgica deve ser coordenada com a estrutura, os processos relacionados as intervenções e as atividades durante o período perioperatório imediato. Objetivos: Implementar tecnologia em saúde na rotina da equipe de enfermagem cirúrgica, por meio de procedimento operacional padrão específico à atenção integral de pacientes com COVID-19. Metodologia: Trata-se de um estudo do tipo qualitativo. A técnica eleita para ser utilizada no presente estudo á análise dos itens do POP pelos avaliadores será por meio do método de Pasquali.Resultados: O cálculo da totalidade do instrumento foi realizado a partir da soma de todos os itens calculados separadamente para obter a média do percentual alcançado. A validação do conteúdo alcançou pontuação máxima de 4 (aplicável) pelos sete juízes nos respectivos quesitos: objetividade, pertinência e precisão com média 28 em cada critério. Conclusão: O presente estudo torna-se relevante em razão da necessidade de elaboração e validação de um POP completo tanto para a execução das atividades, quanto para estabelecer um padrão de qualidade na execução técnica.
In this work, the viability of recycling newspaper for producing cellulose acetate was tested. Newspaper recycling is extremely important not only for the environment preservation, but also from the ...economical point of view of aggregating value to this residue. Cellulose acetate was produced from a homogeneous acetylation, and then characterized by FTIR, DSC and TGA. Acetylation times were 48
h for as received newspaper (CA48) and 24
h for delignified newspaper (CA24), resulting in cellulose diacetate (DS
=
1.98
±
0.22) for CA48 and cellulose triacetate (DS
=
2.79
±
0.02) for CA24, respectively. Membranes of these materials were produced and characterized according to the previously mentioned techniques and by measurements of water vapor flux, which were compared to membranes of nanofiltration SG from Osmonix
®. Results showed that independently of a purification step, it is possible to produce cellulose acetate membranes through the chemical recycling of newspaper and that membrane CA24 presents thermal stability comparable to membranes produced of commercial cellulose acetate.
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