Methylation of 16S ribosomal RNA (rRNA) has recently emerged as a new mechanism of resistance against aminoglycosides among gram-negative pathogens belonging to the family Enterobacteriaceae and ...glucose-nonfermentative microbes, including Pseudomonas aeruginosa and Acinetobacter species. This event is mediated by a newly recognized group of 16S rRNA methylases, which share modest similarity to those produced by aminoglycoside-producing actinomycetes. Their presence confers a high level of resistance to all parenterally administered aminoglycosides that are currently in clinical use. The responsible genes are mostly located on transposons within transferable plasmids, which provides them with the potential to spread horizontally and may in part explain the already worldwide distribution of this novel resistance mechanism. Some of these organisms have been found to coproduce extended-spectrum β-lactamases or metallo-β-lactamases, contributing to their multidrug-resistant phenotypes. A 2-tiered approach, consisting of disk diffusion tests followed by confirmation with polymerase chain reaction, is recommended for detection of 16S rRNA methylase-mediated resistance.
We investigated the association between glucose tolerance status defined by a 75-g oral glucose tolerance test (OGTT) and the development of dementia.
A total of 1,017 community-dwelling ...dementia-free subjects aged ≥60 years who underwent the OGTT were followed up for 15 years. Outcome measure was clinically diagnosed dementia.
The age- and sex-adjusted incidence of all-cause dementia, Alzheimer disease (AD), and vascular dementia (VaD) were significantly higher in subjects with diabetes than in those with normal glucose tolerance. These associations remained robust even after adjustment for confounding factors for all-cause dementia and AD, but not for VaD (all-cause dementia: adjusted hazard ratio HR = 1.74, 95% confidence interval CI = 1.19 to 2.53, p = 0.004; AD: adjusted HR = 2.05, 95% CI = 1.18 to 3.57, p = 0.01; VaD: adjusted HR = 1.82, 95% CI = 0.89 to 3.71, p = 0.09). Moreover, the risks of developing all-cause dementia, AD, and VaD significantly increased with elevated 2-hour postload glucose (PG) levels even after adjustment for covariates, but no such associations were observed for fasting plasma glucose (FPG) levels: compared with those with 2-hour PG levels of <6.7 mmol/L, the multivariable-adjusted HRs of all-cause dementia and AD significantly increased in subjects with 2-hour PG levels of 7.8 to 11.0 mmol/L or over, and the risk of VaD was significantly higher in subjects with levels of ≥11.1 mmol/L.
Our findings suggest that diabetes is a significant risk factor for all-cause dementia, AD, and probably VaD. Moreover, 2-hour PG levels, but not FPG levels, are closely associated with increased risk of all-cause dementia, AD, and VaD.
High molecular weight polyhydroxyalkanoates (PHA) are synthesized and stored in the cell cytoplasm as water-insoluble inclusions by various microorganisms. This intriguing biological polyester ...initially attracted the attention of microbiologists and managed to keep many polymer scientists occupied over the second half of the last century. Concerted multidisciplinary scientific approaches have been directed to the elucidation of various aspects of PHA. Of significant interest are the findings that PHA can consist of various hydroxyalkanoate monomers, and the cloning of its biosynthesis genes. This has resulted in the production of PHA with various physical properties by genetically engineered microorganisms. In fact, it is now possible that large-scale production of PHA by transgenic plants can be achieved in the near future. The physical properties of PHA homopolymers as well as co- and heteropolymers have been the subject of study in various laboratories all over the world. By controlling the monomer composition of PHA, polymer scientists have shown that the polymer's physical properties can be regulated to a great extent. Furthermore, it is also clear that the rate of degradation of PHA in various environments can be controlled by judiciously altering its monomer compositions. This review attempts to bring together the biochemical and physicochemical aspects of PHA along with new perspectives on its potential therapeutic applications.
Context.
Despite recent observational and theoretical advances in mapping the magnetic fields associated with molecular clouds, their three-dimensional (3D) morphology remains unresolved. ...Multi-wavelength and multi-scale observations will allow us to paint a comprehensive picture of the magnetic fields of these star-forming regions.
Aims.
We reconstructed the 3D magnetic field morphology associated with the Perseus molecular cloud and compared it with predictions of cloud-formation models. These cloud-formation models predict a bending of magnetic fields associated with filamentary molecular clouds. We compared the orientation and direction of this field bending with our 3D magnetic-field view of the Perseus cloud.
Methods.
We used previous line-of-sight and plane-of-sky magnetic field observations as well as Galactic magnetic field models to reconstruct the complete 3D magnetic field vectors and morphology associated with the Perseus cloud.
Results.
We approximated the 3D magnetic field morphology of the cloud as a concave arc that points in the decreasing longitude direction in the plane of the sky (from our point of view). This field morphology preserves a memory of the Galactic magnetic field. In order to compare this morphology to cloud-formation model predictions, we assume that the cloud retains a memory of its most recent interaction. After incorporating velocity observations, we find that the line-of-sight magnetic field observations are consistent with predictions of shock-cloud-interaction models.
Conclusions.
To our knowledge, this is the first time that the 3D magnetic fields of a molecular cloud have been reconstructed. We find the 3D magnetic field morphology of the Perseus cloud to be consistent with the predictions of the shock-cloud-interaction model that describes the formation mechanism of filamentary molecular clouds.
Magnetic fields permeate the interstellar medium and are important in the star formation process. Determining the three-dimensional (3D) magnetic fields of molecular clouds will allow us to better ...understand their role in the evolution of these clouds and the formation of stars. We fully reconstruct the approximate 3D magnetic field morphology of the Orion A molecular cloud (on scales of a few to ∼100 pc) using Galactic magnetic field models, as well as available line-of-sight and plane-of-sky magnetic field observations. While previous studies identified the 3D magnetic field morphology of the Orion A cloud as an arc shape, in this study we provide the orientation of this arc-shaped field and its plane-of-sky direction for the first time. We find that this 3D field is a tilted, semi-convex (from our point of view) structure and mostly points in the direction of decreasing latitude and longitude on the plane of the sky from our vantage point. The previously identified bubbles and events in this region were key in shaping this arc-shaped magnetic field morphology.
The rising prevalence of antibiotic resistance underscores the urgent need for novel antimicrobial agents. Antimicrobial peptides (AMPs) are potentially effective therapeutics that disrupt bacterial ...membranes regardless of resistance to traditional antibiotics. We have developed engineered cationic AMPs (eCAPs) with broad activity against multidrug-resistant (MDR) bacteria, but stability remains an important concern. Therefore, we sought to enhance the clinical utility of eCAP WLBU2 in biological matrices relevant to respiratory infection. A designed substitution of d-Val for l-Val resulted in increased resistance to protease enzymatic degradation. We observed multiple gains of functions such as higher activity against bacteria in biofilm mode of growth, significantly lower toxicity to erythrocytes and white blood cells compared to WLBU2, with increased safety in mice. Direct airway delivery revealed a therapeutic index of >140 for the selected enantiomer compared to that of <35 for WLBU2. The data warrant clinical exploration by aerosolized delivery to mitigate MDR-related respiratory infection.
Context. Circumstellar disks are expected to evolve quickly in massive young clusters harboring many OB-type stars. Two processes have been proposed to drive the disk evolution in such cruel ...environments: (1) gravitational interaction between circumstellar disks and nearby passing stars (stellar encounters); and (2) photoevaporation by UV photons from massive stars. The relative importance of both mechanisms is not well understood. Studies of massive young star clusters can provide observational constraints on the processes of driving disk evolution. Aims. We investigate the properties of young stars and their disks in the NGC 6357 complex, concentrating on the most massive star cluster within the complex: Pismis 24. Methods. We use infrared data from the 2MASS and Spitzer GLIMPSE surveys, complemented with our own deep Spitzer imaging of the central regions of Pismis 24, in combination with X-ray data to search for young stellar objects (YSOs) in NGC 6357 complex. The infrared data constrain the disk presence and are complemented by optical photometric and spectroscopic observations, obtained with VLT/VIMOS, that constrain the properties of the central stars. For those stars with reliable spectral types we combine spectra and photometry to estimate the mass and age. For cluster members without reliable spectral types we obtain the mass and age probability distributions from R and I-band photometry, assuming these stars have the same extinction distribution as those in the “spectroscopic” sample. We compare the disk properties in the Pismis 24 cluster with those in other clusters/star-forming regions employing infrared color–color diagrams. Results. We discover two new young clusters in the NGC 6357 complex. We give a revised distance estimate for Pismis 24 of 1.7 ± 0.2 kpc. We find that the massive star Pis 24-18 is a binary system, with the secondary being the main X-ray source of the pair. We provide photometry in 9 bands between 0.55 and 9 μm for the members of the Pismis 24 cluster. We derive the cluster mass function and find that up to the completeness limit at low masses it agrees well with the initial mass function of the Trapezium cluster. We derive a median age of 1 Myr for the Pismis 24 cluster members. We find five proplyds in HST archival imaging of the cluster, four of which are newly found. In all cases the proplyd tails are pointing directly away from the massive star system Pis 24-1. One proplyd shows a second tail, pointing away from Pis 24-2, suggesting this object is being photoevaporated from two directions simultaneously. We find that the global disk frequency (~30%) in Pismis 24 is much lower than some other clusters of similar age, such as the Orion Nebula Cluster. When comparing the disk frequencies in 19 clusters/star-forming regions of various ages and different (massive) star content, we find that the disks in clusters harboring extremely massive stars (typically earlier than O5), like Pismis 24, are dissipated roughly twice as quickly as in clusters/star-forming regions without extremely massive stars. Within Pismis 24, we find that the disk frequency within a projected distance of 0.6 pc from Pis 24-1 is substantially lower than at larger radii (~19% vs. ~37%). We argue for a combination of photoevaporation and irradiation with ionizing UV photons from nearby massive stars, causing increased MRI-induced turbulence and associated accretion activity, to play an important role in the dissipation of low-mass star disks in Pismis 24.
Aims
Early studies have shown that magnesium intake decreases the risk of Type 2 diabetes, but the results are still inconsistent. We prospectively examined the association between magnesium intake ...and incidence of Type 2 diabetes in a general Japanese population.
Methods
A total of 1999 subjects without diabetes aged 40–79 years who underwent a 75‐g oral glucose tolerance test were followed up prospectively for a mean of 15.6 years.
Results
During the follow‐up, 417 subjects developed Type 2 diabetes. The age‐ and sex‐adjusted incidence of Type 2 diabetes significantly decreased with increasing magnesium intake quartile levels (≤ 148.5, 148.6–171.5, 171.6–195.5 and ≥ 195.6 mg/day, P for trend = 0.01). In multivariate analyses, after adjusting for comprehensive risk factors and other dietary factors, the hazard ratio of Type 2 diabetes was 0.67 (95% CI 0.49–0.92; P = 0.01) in the third quartile and 0.63 (95% CI 0.44–0.90; P = 0.01) in the highest quartile compared with the first quartile. In addition, the risk of Type 2 diabetes was 14% lower (P = 0.04) for a 1‐sd increment of log‐transformed magnesium intake in the multivariate‐adjusted model. In stratified analysis, there were statistically significant interactions between magnesium intake and levels of homeostasis model assessment of insulin resistance, high‐sensitivity C‐reactive protein or alcohol intake on the risk of Type 2 diabetes (all P < 0.05).
Conclusions
Our findings suggest that increased magnesium intake was a significant protective factor for the incidence of Type 2 diabetes in the general Japanese population, especially among subjects with insulin resistance, low‐grade inflammation and a drinking habit.
What's new?
A number of prospective cohort studies and meta‐analyses have suggested that magnesium intake reduces the risk of diabetes, but the results are still inconsistent. In addition, most cohort studies have been derived from Caucasian populations, and thus the effect of magnesium intake on diabetes risk in Asians remains to be fully elucidated. In this paper, we demonstrate that higher intake of magnesium reduces the risk of Type 2 diabetes in Japanese.
To the best of our knowledge, this is the first prospective study showing the effect modification by insulin resistance and low‐grade inflammation on the association between magnesium intake and the development of Type 2 diabetes.
•Large, cryogenic magnetocaloric effect reported for rare-earth ferrite-type compounds EuGd2O4 and EuDy2O4.•Magnetocaloric effect directly measured and obtained from magnetization and heat capacity ...measurements.•High magnetic density and low ordering temperatures for EuGd2O4 and EuDy2O4.•Magnetic ordering mechanisms explained semi-quantitatively with mean-field simulations.
Magnetization, heat capacity and direct measurements of the magnetocaloric effect show that EuGd2O4 and EuDy2O4 have a remarkably large magnetocaloric effect at cryogenic temperatures, owing to their high magnetic density and low ordering temperatures. The Gd derivative orders antiferromagnetically at TN = 4.6 K, while its magnetocaloric effect largely exceeds that of the reference refrigerant Gadolinium Gallium Garnet (GGG) above 5 K. The Dy derivative undergoes two phase transitions at TC1 = 3.65 K and TC2 = 4.7 K, respectively, which are the result of a peculiar magnetic arrangement: the first Dy sublattice is parallel to the crystallographic c-axis, while the Eu sublattice makes a variable angle from 0o to 45o with the direction of the second Dy sublattice that lies in the ab-plane. EuDy2O4 has a lower magnetocaloric effect than EuGd2O4, yet larger than GGG. Both ordering mechanisms are semi-quantitatively explained within the frame of a mean-field simulation, which takes into account the magnetic anisotropy strength of the participating magnetic ions.
We present a new catalog of 118 ultraluminous infrared galaxies (ULIRGs) and one hyperluminous infrared galaxy (HLIRG) by cross-matching the AKARI all-sky survey with the Sloan Digital Sky Survey ...Data Release 10 (SDSS DR10) and the final data release of the Two-Degree Field Galaxy Redshift Survey. Forty of the ULIRGs and one HLIRG are new identifications. We find that ULIRGs are interacting pair galaxies or ongoing or postmergers. This is consistent with the widely accepted view: ULIRGs are major mergers of disk galaxies. We confirm the previously known positive trend between the active galactic nucleus fraction and infrared luminosity. We show that ULIRGs have a large offset from the main sequence up to z ~ 1; their offset from the z ~ 2 "main sequence" is relatively smaller. We find a result consistent with the previous studies showing that, compared to local starforming SDSS galaxies of similar mass, local ULIRGs have lower oxygen abundances. We demonstrate for the first time that ULIRGs follow the fundamental metallicity relation (FMR). The scatter of ULIRGs around the FMR (0.09 dex-0.5 dex) is comparable to the scatter of z ~ 2-3 galaxies. We provide the largest local (0.050 < z < 0.487) ULIRG catalog with stellar masses, star-formation rates, gas metallicities, and optical colors.
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