Bdelloid rotifers are micro-invertebrates distributed worldwide, from temperate latitudes to the most extreme areas of the planet like Antarctica or the Atacama Desert. They have colonized any ...habitat where liquid water is temporarily available, including terrestrial environments such as soils, mosses, and lichens, tolerating desiccation and other types of stress such as high doses of ionizing radiation (IR). It was hypothesized that bdelloid desiccation and radiation resistance may be attributed to their potential ability to repair DNA double-strand breaks (DSBs). Here, these properties are investigated and compared among nine bdelloid species collected from both mild and harsh habitats, addressing the correlation between the ability of bdelloid rotifers to survive desiccation and their capacity to repair massive DNA breakage in a phylogenetically explicit context. Our research includes both specimens isolated from habitats that experience frequent desiccation (at least 1 time per generation), and individuals sampled from habitats that rarely or never experienced desiccation.
Our analysis reveals that DNA repair prevails in somatic cells of both desiccation-tolerant and desiccation-sensitive bdelloid species after exposure to X-ray radiation. Species belonging to both categories are able to withstand high doses of ionizing radiation, up to 1000 Gy, without experiencing any negative effects on their survival. However, the fertility of two desiccation-sensitive species, Rotaria macrura and Rotaria rotatoria, was more severely impacted by low doses of radiation than that of desiccation-resistant species. Surprisingly, the radioresistance of desiccation-resistant species is not related to features of their original habitat. Indeed, bdelloids isolated from Atacama Desert or Antarctica were not characterized by a higher radioresistance than species found in more temperate environments.
Tolerance to desiccation and radiation are supported as ancestral features of bdelloid rotifers, with a group of species of the genus Rotaria having lost this trait after colonizing permanent water habitats. Together, our results provide a comprehensive overview of the evolution of desiccation and radiation resistance among bdelloid rotifers.
Invasive bivalves continue to spread and negatively impact freshwater ecosystems worldwide. As different metrics for body size and biomass are frequently used within the literature to standardise ...bivalve‐related ecological impacts (e.g. respiration and filtration rates), the lack of broadly applicable conversion equations currently hinders reliable comparison across bivalve populations. To facilitate improved comparative assessment among studies originating from disparate geographical locations, we report body size and biomass conversion equations for six invasive freshwater bivalves (or species complex members) worldwide: Corbicula fluminea, C. largillierti, Dreissena bugensis, D. polymorpha, Limnoperna fortunei and Sinanodonta woodiana, and tested the reliability (i.e. precision and accuracy) of these equations.
Body size (length, width and height) and biomass metrics of living‐weight (LW), wet‐weight (WW), dry‐weight (DW), dry shell‐weight (SW), shell free dry‐weight (SFDW) and ash‐free dry‐weight (AFDW) were collected from a total of 44 bivalve populations located in Asia, the Americas and Europe. Relationships between body size and individual biomass metrics, as well as proportional weight‐to‐weight conversion factors, were determined.
For most species, although inherent variation existed between sampled populations, body size directional measurements were found to be good predictors of all biomass metrics (e.g. length to LW, WW, SW or DW: R2 = 0.82–0.96), with moderate to high accuracy for mean absolute error (MAE): ±9.14%–24.19%. Similarly, narrow 95% confidence limits and low MAE were observed for most proportional biomass relationships, indicating high reliability for the calculated conversion factors (e.g. LW to AFDW; CI range: 0.7–2.0, MAE: ±0.7%–2.0%).
Synthesis and applications. Our derived biomass prediction equations can be used to rapidly estimate the biologically active biomass of the assessed species, based on simpler biomass or body size measurements for a wide range of situations globally. This allows for the calculation of approximate average indicators that, when combined with density data, can be used to estimate biomass per geographical unit‐area and contribute to quantification of population‐level effects. These general equations will support meta‐analyses, and allow for comparative assessment of historic and contemporary data. Overall, these equations will enable conservation managers to better understand and predict ecological impacts of these bivalves.
Our derived biomass prediction equations can be used to rapidly estimate the biologically active biomass of the assessed species, based on simpler biomass or body size measurements for a wide range of situations globally. This allows for the calculation of approximate average indicators that, when combined with density data, can be used to estimate biomass per geographical unit‐area and contribute to quantification of population‐level effects. These general equations will support meta‐analyses, and allow for comparative assessment of historic and contemporary data. Overall, these equations will enable conservation managers to better understand and predict ecological impacts of these bivalves.
Development of RE1/1 RPCs for the CMS muon trigger system Park, S.; Ahn, S.H.; Akimenko, S. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
05/2009, Letnik:
602, Številka:
3
Journal Article
Recenzirano
The first six Resistive Plate Chambers (RPCs) of RE1/1 in the forward region of the Compact Muon Solenoid (CMS) detector were constructed and are being tested. These RPCs cover the pseudo-rapidity ...region from 1.6 to 2.1 and will serve as the base detector for the CMS RPC muon trigger. We report that these six RPCs are being tested with the CMS RPC quality certification procedures and that a full measurement of a chamber efficiency of
95
±
4
%
has been achieved at an operating voltage of 9.4
kV. This performance demonstrates that the RPC is qualified to be tested at the closest distance to the beam pipe as the muon trigger detector at the full LHC design luminosity.
This paper introduces a novel production technique for the planar wire grid plates of microstrip gas chambers (MSGCs, gaseous particle detectors), taking advantage from the typical thin film ...metallization technologies. The aim of the work here presented has been to manufacture MSGC substrates which can meet the challenging requirements proper to particle tracking detectors in highly ionizing environments. These requirements are mainly represented by high reliability and robustness, since the devices are supposed to operate efficiently for at least 10 years in the extremely hostile conditions foreseen at the large hadron collider at CERN (CH). A detailed process description will be given and results will be analyzed both from the production point of view and from the application of the manufactured prototypes as detectors for high energy physics experiments.
The CMS experiment comprises MSGCs as one of the key detection elements for high luminosity tracking at LHC. In _addition to the high dose rate of 10 mC/year per cm of strip, these detectors have to ...survive the hostile presence of highly ionizing particles, neutrons low energy gammas and hadrons. In this report we present the results of systematic tests on maximum safe operational gain limits in MSGCs before the discharge. Long term ageing tests performed on prototype open ‘banana’ modules envisaged to be arranged around the interaction region in the forward part of the CMS tracker show no evidence of gain drop up to equivalent ∼ 10 years of LHC operation. A comparison is made between argon and neon gas mixtures with DME in equal proportions by investigating long term irradiation effocts on chamber operation by introducing controlled and reproducible pollution in the gas lines.
A search for pair-production of first generation scalar leptoquarks is performed in the final state containing an electron, a neutrino, and at least two jets using proton-proton collision data at ...inline image. The data were collected by the CMS detector at the LHC, corresponding to an integrated luminosity of 36 pb super(-1. The number of observed events is in good agreement with the predictions for standard model processes. Prior CMS results in the dielectron channel are combined with this electron + neutrino search. A 95% confidence level combined lower limit is set on the mass of a first generation scalar leptoquark at 339 GeV for beta=0.5, where beta is the branching fraction of the leptoquark to an electron and a quark. These results represent the most stringent direct limits to date for values of beta greater than 0.05.)