Arachidonic acid (ARA) is an important ω-6 polyunsaturated fatty acid (PUFA), and docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and n-3 docosapentaenoic acid (n-3 DPA) are three well-known ...ω-3 PUFAs. These fatty acids can be metabolized into a number of bioactive lipids. Eicosanoids derived from ARA have drawn great attention because of their important and complex biofunctions. Although EPA, DHA and n-3 DPA have also shown powerful biofunctions, we have fewer studies of metabolites derived from them than those from ARA. Recently, growing research has focused on the bioaction of ω-3 PUFA-derived metabolites, which indicates their great potential for treating metabolic disorders. Most of the functional studies of these bioactive lipids focused on their anti-inflammatory effects. However, several studies elucidated their direct effects on pancreatic β cells, hepatocytes, adipocytes, skeletal muscle cells, and endothelial cells. These researches revealed the importance of studying the functions of metabolites derived from ω-3 polyunsaturated fatty acids other than themselves. The current review summarizes research into the effects of ω-3 PUFA-derived oxylipins on metabolic disorders, including diabetes, non-alcoholic fatty liver disease, adipose tissue dysfunction, and atherosclerosis.
To explore the rule of airflow-dust-gas dispersion and interaction in a fully mechanized mining face, the airflow current vector, the dust trajectory, and the gas spatial distribution were ...numerically simulated by Fluent. The results show that under the influence of airflow, respiratory dust diffuses to the leeward side of each dust-producing point and footway space and forms a high-concentration (peak concentration 2000 mg/m
3
) dust mass at 2 m on the leeward side of the advancing support. Gas tends to accumulate near the coal cutter drum, the roof, and the return air corner of the mining face, and the peak concentration exceeds the lower limit of explosion. Near the rear drum of the coal cutter and at the advancing support, considering that the gas and coal dust concentrations are both high and dust and gas can reduce each other’s explosion limit, serious gas and coal dust explosions are extremely likely to occur in the presence of a fire source, which may result in serious consequences. Therefore, the two areas can be regarded as key areas of gas and coal dust explosion prevention and control.
Aging is a major risk factor for metabolic disorders. Polyunsaturated fatty acid-derived bioactive lipids play critical roles as signaling molecules in metabolic processes. Nonetheless, their effects ...on age-related liver steatosis remain unknown. Here we show that senescent liver cells induce liver steatosis in a paracrine manner. Linoleic acid-derived 9-hydroxy-octadecadienoic acid (9-HODE) and 13-HODE increase in middle-aged (12-month-old) and aged (20-month-old) male mouse livers and conditioned medium from senescent hepatocytes and macrophages. Arachidonate 15-lipoxygenase, an enzyme for 13-HODE and 9-HODE production, is upregulated in senescent cells. A 9-HODE and 13-HODE mixture induces liver steatosis and activates SREBP1. Furthermore, catalase (CAT) is a direct target of 13-HODE, and its activity is decreased by 13-HODE. CAT overexpression reduces 13-HODE-induced liver steatosis and protects male mice against age-related liver steatosis. Therefore, 13-HODE produced by senescent hepatocytes and macrophages activates SREBP1 by directly inhibiting CAT activity and promotes liver steatosis.
Tail gas emitted by underground trackless rubber wheel cars poses a serious threat to the health and safety of underground workers. To effectively reduce the tail gas concentration of a comprehensive ...excavation face, this study adopted a numerical simulation method to investigate the influence of air suction volume Q and distance L between trackless rubber wheel cars and headfaces on the diffusion law of diesel particulate matter, CO, and NO
under long suction and short pressure ventilation. The results showed that under the condition of L = 20 m, the trackless rubber wheel car is closer to the suction air duct. At this point, when Q = 600 m
/min, the tail gas control effect in the roadway is optimum. In addition, under the condition of L = 40 m, the trackless rubber wheel car is in the middle of the roadway. At this point, when Q = 300 m
/min, the tail gas control effect in the roadway is optimum. When L = 60 m and Q = 200 m
/min, the ventilation mode in the roadway is mainly pressure-in ventilation. The high-volume-fraction NO
region and the medium-volume-fraction NO
region under this air volume are small.
Cytosine DNA methylation is an important epigenetic modification termed as the fifth base that functions in diverse processes. Till now, the genome-wide DNA methylation maps of many organisms has ...been reported, such as human, Arabidopsis, rice and silkworm, but the methylation pattern of bird remains rarely studied. Here we show the genome-wide DNA methylation map of bird, using the chicken as a model organism and an immunocapturing approach followed by high-throughput sequencing. In both of the red jungle fowl and the avian broiler, DNA methylation was described separately for the liver and muscle tissue. Generally, chicken displays analogous methylation pattern with that of animals and plants. DNA methylation is enriched in the gene body regions and the repetitive sequences, and depleted in the transcription start site (TSS) and the transcription termination site (TTS). Most of the CpG islands in the chicken genome are kept in unmethylated state. Promoter methylation is negatively correlated with the gene expression level, indicating its suppressive role in regulating gene transcription. This work contributes to our understanding of epigenetics in birds.
Targeting the white-to-brown fat conversion is important for developing potential strategies to counteract metabolic diseases; yet the mechanisms are not fully understood. Yes-associated-protein ...(YAP), a transcription co-activator, was demonstrated to regulate adipose tissue functions; however, its effects on browning of subcutaneous white adipose tissue (sWAT) are unclear. We demonstrated that YAP was highly expressed in cold-induced beige fat. Mechanistically, YAP was found as a target gene of miR-429, which downregulated YAP expression
and
In addition, miR-429 level was decreased in cold-induced beige fat. Additionally, pharmacological inhibition of the interaction between YAP and transcriptional enhanced associate domains by verteporfin dampened the browning of sWAT. Although adipose tissue-specific YAP overexpression increased energy expenditure with increased basal uncoupling protein 1 expression, it had no additional effects on the browning of sWAT in young mice. However, we found age-related impairment of sWAT browning along with decreased YAP expression. Under these circumstances, YAP overexpression significantly improved the impaired WAT browning in middle-aged mice. In conclusion, YAP as a regulator of sWAT browning, was upregulated by lowering miR-429 level in cold-induced beige fat. Targeting the miR-429-YAP pathway could be exploited for therapeutic strategies for age-related impairment of sWAT browning.
Myocardial infarction (MI) is a fatal manifestation of coronary heart disease, and its underlying mechanism is still largely unknown. Lipid levels and composition alterations predict the risk of MI ...complications. Glycerophospholipids (GPLs) are important bioactive lipids and play a crucial role in the development of cardiovascular diseases. However, the metabolic changes in the GPLs profile during post-MI injury remain unknown.
In the current study, we constructed a classic MI model by ligating the left anterior descending branch and assessed the alterations in both plasma and myocardial GPLs profiles during the reparative phase post-MI by liquid chromatography-tandem mass spectrometry analysis.
We found that myocardial GPLs, but not plasma GPLs, were markedly changed after MI injury. Importantly, MI injury is associated with decreased phosphatidylserine (PS) levels. Consistently, the expression of phosphatidylserine synthase 1 (PSS1), which catalyzes the formation of PS from its substrate phosphatidylcholine, was significantly reduced in heart tissues after MI injury. Furthermore, oxygen-glucose deprivation (OGD) inhibited PSS1 expression and reduced PS levels in primary neonatal rat cardiomyocytes, while overexpression of PSS1 restored the inhibition of PSS1 and the reduction in PS levels caused by OGD. Moreover, overexpression of PSS1 abrogated, whereas knockdown of PSS1 aggravated, OGD-induced cardiomyocyte apoptosis.
Our findings revealed that GPLs metabolism was involved in the reparative phase post-MI, and cardiac decreased PS levels, resulting from inhibition of PSS1, are important contributor to the reparative phase post-MI. PSS1 overexpression represents a promising therapeutic strategy to attenuate MI injury.
As their service life increases, cement-based materials inevitably undergo microcracking and local damage. In response to this problem, this study used phacoemulsification-solvent volatilization to ...prepare a multifunctional sustained-release microcapsule (SFRM) with self-healing and flame-retardant characteristics. The synthesis of SFRM is based on the modification of ethyl cellulose with nano-SiO2 particles and cross-linking with a silane coupling agent to form an organic–inorganic hybrid wall material. The epoxy resin is blended with hexaphenoxy cyclotriphosphazene (HPCTP) to form a composite core emulsion. The surface morphology, particle size distribution, core–shell composition, and thermal stability of SFRM were analyzed via scanning electron microscopy (SEM), energy-dispersive spectrometry (EDS), Malvern, Fourier-transform infrared (FT-IR), and TD-DSC-DTG. It is concluded that SFRM was successfully synthesized with superior particle size distribution and thermal stability. When the ratio of SiO2 solution and EC alcohol solution reached 1:2, the particle size distribution of the microcapsules was 30–190 μm, and the D 50 decreased to 70 μm. The core material content, slow-release performance, and flame retardancy of SFRM were measured using a UV-1800 spectrophotometer and Hartmann tubes, and the compressive and repair properties of SFRM were evaluated by uniaxial compression tests. The results demonstrate that SFRM has satisfactory slow-release and flame-retardancy properties, the LC is 67%, and the first-order kinetic model shows the best fit and conforms to the non-Fickian diffusion mechanism. The SFRM repair rate can reach approximately 61%. This is of substantial significance to the field of self-repairing cement-based materials.
Alternatively spliced genes produce multiple spliced isoforms, called transcript variants. In differential alternative splicing, transcript variant abundance differs across sample types. Differential ...alternative splicing is common in animal systems and influences cellular development in many processes, but its extent and significance is not as well known in plants. To investigate differential alternative splicing in plants, we examined RNA‐Seq data from rice seedlings. The data included three biological replicates per sample type, approximately 30 million sequence alignments per replicate, and four sample types: roots and shoots treated with exogenous cytokinin delivered hydroponically or a mock treatment. Cytokinin treatment triggered expression changes in thousands of genes but had negligible effect on splicing patterns. However, many genes were differentially spliced between mock‐treated roots and shoots, indicating that our methods were sufficiently sensitive to detect differential splicing between data sets. Quantitative fragment analysis of reverse transcriptase‐PCR products made from newly prepared rice samples confirmed 9 of 10 differential splicing events between rice roots and shoots. Differential alternative splicing typically changed the relative abundance of splice variants that co‐occurred in a data set. Analysis of a similar (but less deeply sequenced) RNA‐Seq data set from Arabidopsis showed the same pattern. In both the Arabidopsis and rice RNA‐Seq data sets, most genes annotated as alternatively spliced had small minor variant frequencies. Of splicing choices with abundant support for minor forms, most alternative splicing events were located within the protein‐coding sequence and maintained the annotated reading frame. A tool for visualizing protein annotations in the context of genomic sequence (ProtAnnot) together with a genome browser (Integrated Genome Browser) were used to visualize and assess effects of differential splicing on gene function. In general, differentially spliced regions coincided with conserved protein domains, indicating that differential alternative splicing is likely to affect protein function between root and shoot tissue in rice.
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