Stroke is the second most deadly disease globally and seriously endangers people's lives and health. The automatic detection of stroke lesions from diffusion-weighted imaging (DWI) can improve the ...diagnosis. Recently, automatic detection methods based on YOLOv5 have been utilized in medical images. However, most of them barely capture the stroke lesions because of their small size and fuzzy boundaries.
To address this problem, a novel method for tracing the edge of the stroke lesion based on YOLOv5 (TE-YOLOv5) is proposed. Specifically, we constantly update the high-level features of the lesion using an aggregate pool (AP) module. Conversely, we feed the extracted feature into the reverse attention (RA) module to trace the edge relationship promptly. Overall, 1681 DWI images of 319 stroke patients have been collected, and experienced radiologists have marked the lesions. DWI images were randomly split into the training and test set at a ratio of 8:2. TE-YOLOv5 has been compared with the related models, and a detailed ablation analysis has been conducted to clarify the role of the RA and AP modules.
TE-YOLOv5 outperforms its counterparts and achieves competitive performance with a precision of 81.5%, a recall of 75.8%, and a mAP@0.5 of 80.7% (mean average precision while the intersection over union is 0.5) under the same backbone. At the patient level, the positive finding rate can reach 98.51%, while the confidence is set at 80.0%. After ablating RA, the mAP@0.5 decreases to 79.6%; after ablating RA and AP, the mAP@0.5 decreases to 78.1%.
The proposed TE-YOLOv5 can automatically and effectively detect stroke lesions from DWI images, especially for those with an extremely small size and blurred boundaries. AP and RA modules can aggregate multi-layer high-level features and concurrently track the edge relationship of stroke lesions. These detection methods might help radiologists improve stroke diagnosis and have great application potential in clinical practice.
•Improved YOLOv5 used to detect stroke lesions from diffusion-weighted imaging.•An aggregate pool module enables the update of high-level lesion features.•A reverse attention module is added to trace the edge relationship of lesions.•Mean average precision while the intersection over union is 0.5 reaches 81.1%.•Stroke lesions with a small size and blurred boundaries can be detected.
Increasing evidences have shown that long non-coding RNAs (lncRNAs) display crucial regulatory roles in the occurrence and development of numerous diseases. However, the function and underlying ...mechanisms of lncRNAs in hypertrophy of ligamentum flavum (HLF) have not been report.
The integrated analysis of lncRNAs sequencing, bioinformatics analysis and real-time quantitative PCR were used to identify the key lncRNAs involved in HLF progression. Gain- and loss-function experiments were used to explore the functions of lncRNA X inactive specific transcript (XIST) in HLF. Mechanistically, bioinformatics binding site analysis, RNA pull-down, dual-luciferase reporter assay, and rescue experiments were utilized to investigate the mechanism by which XIST acts as a molecular sponge of miR-302b-3p to regulate VEGFA-mediated autophagy.
We identified that XIST was outstandingly upregulated in HLF tissues and cells. Moreover, the up-regulation of XIST strongly correlated with the thinness and fibrosis degree of LF in LSCS patients. Functionally, knockdown of XIST drastically inhibited proliferation, anti-apoptosis, fibrosis and autophagy of HLF cells in vitro and suppressed hypertrophy and fibrosis of LF tissues in vivo. Intestinally, we uncovered that overexpression of XIST significantly promoted proliferation, anti-apoptosis and fibrosis ability of HLF cells by activating autophagy. Mechanistic studies illustrated that XIST directly medullated the VEGFA-mediated autophagy through sponging miR-302b-3p, thereby enhancing the development and progression of HLF.
Our findings highlighted that the XIST/miR-302b-3p/VEGFA-mediated autophagy axis is involved in development and progression of HLF. At the same time, this study will complement the blank of lncRNA expression profiles in HLF, which laid the foundation for further exploration of the relationship between lncRNAs and HLF in the future.
Hypertrophy of ligamentum flavum (HLF) is a common lumbar degeneration disease (LDD) with typical symptoms of low back pain and limb numbness owing to an abnormal pressure on spinal nerves. Previous ...studies revealed HLF might be caused by fibrosis, inflammatory, and other bio-pathways. However, a global analysis of HLF is needed severely.
A genome-wide DNA methylation and single-nucleotide polymorphism analysis were performed from five LDD patients with HLF and five LDD patients without HLF. Comprehensive integrated analysis was performed using bioinformatics analysis and the validated experiments including Sanger sequencing, methylation-specific PCR, qPCR and ROC analysis. Furthermore, the function of novel genes in ligamentum flavum cells (LFCs) was detected to explore the molecular mechanism in HLF through knock down experiment, overexpression experiment, CCK8 assay, apoptosis assay, and so on.
We identified 69 SNP genes and 735 661 differentially methylated sites that were enriched in extracellular matrix, inflammatory, and cell proliferation. A comprehensive analysis demonstrated key genes in regulating the development of HLF including ACSM5. Furthermore, the hypermethylation of ACSM5 that was mediated by DNMT1 led to downregulation of ACSM5 expression, promoted the proliferation and fibrosis, and inhibited the apoptosis of LFCs.
This study revealed that DNMT1/ACSM5 signaling could enhance HLF properties in vitro as a potential therapeutic strategy for HLF.
Background and purpose Malignant brain edema (MBE) occurring after mechanical thrombectomy (MT) in acute ischemic stroke (AIS) could lead to severe disability and mortality. We aimed to investigate ...the incidence, predictors, and clinical outcomes of MBE in patients with AIS after MT. Methods The clinical and imaging data of 155 patients with AIS of anterior circulation after MT were studied. Standard non-contrast CT was used to evaluate baseline imaging characteristics at admission. Clinical outcomes were measured using the 90-day modified Rankin Scale (mRS) score. Based on the follow-up CT scans performed within 72 h after MT, the patients were classified into MBE and non-MBE group. MBE was defined as a midline shift of greater than or equal to 5 mm with signs of local brain swelling. Univariate and multivariate regression analyses were used to analyze the relationship between MBE and clinical outcomes and identify the predictors that correlate with MBE. Results MBE was observed in 19.4% of the patients who underwent MT and was associated with a lower rate of favorable 90-day clinical outcomes. Significant differences were observed in both MBE and non-MBE groups: baseline Alberta Stroke Program Early CT (ASPECT) score, hyperdense middle cerebral artery sign (HMCAS), baseline signs of early infarct, angiographic favorable collaterals, number of retrieval attempts, and revascularization rate. Multivariate analysis indicated that low baseline ASPECT score, absent HMCAS, angiographic poor collaterals, more retrieval attempt count, and poor revascularization independently influenced the occurrence of MBE in AIS patients with anterior circulation after MT. Conclusion MBE was associated with a lower rate of favorable 90-day clinical outcomes. Low baseline ASPECT score, absent HMCAS, angiographic poor collaterals, more retrieval attempt count and poor revascularization were independently associated with MBE after MT. Keywords: Acute ischemic stroke, Malignant brain edema, Mechanical thrombectomy, Large vessel occlusion, Hyperdense middle cerebral artery sign
Female moths synthesize species-specific sex pheromone components and release them to attract male moths, which depend on precise sex pheromone chemosensory system to locate females. Two types of ...genes involved in the sex pheromone biosynthesis and degradation pathways play essential roles in this important moth behavior. To understand the function of genes in the sex pheromone pathway, this study investigated the genome-wide and digital gene expression of sex pheromone biosynthesis and degradation genes in various adult tissues in the diamondback moth (DBM), Plutella xylostella, which is a notorious vegetable pest worldwide.
A massive transcriptome data (at least 39.04 Gb) was generated by sequencing 6 adult tissues including male antennae, female antennae, heads, legs, abdomen and female pheromone glands from DBM by using Illumina 4000 next-generation sequencing and mapping to a published DBM genome. Bioinformatics analysis yielded a total of 89,332 unigenes among which 87 transcripts were putatively related to seven gene families in the sex pheromone biosynthesis pathway. Among these, seven two desaturases (DES), three fatty acyl-CoA reductases (FAR) one acetyltransferase (ACT) and one alcohol dehydrogenase (AD) were mainly expressed in the pheromone glands with likely function in the three essential sex pheromone biosynthesis steps: desaturation, reduction, and esterification. We also identified 210 odorant-degradation related genes (including sex pheromone-degradation related genes) from seven major enzyme groups. Among these genes, 100 genes are new identified and two aldehyde oxidases (AOXs), one aldehyde dehydrogenase (ALDH), five carboxyl/cholinesterases (CCEs), five UDP-glycosyltransferases (UGTs), eight cytochrome P450 (CYP) and three glutathione S-transferases (GSTs) displayed more robust expression in the antennae, and thus are proposed to participate in the degradation of sex pheromone components and plant volatiles.
To date, this is the most comprehensive gene data set of sex pheromone biosynthesis and degradation enzyme related genes in DBM created by genome- and transcriptome-wide identification, characterization and expression profiling. Our findings provide a basis to better understand the function of genes with tissue enriched expression. The results also provide information on the genes involved in sex pheromone biosynthesis and degradation, and may be useful to identify potential gene targets for pest control strategies by disrupting the insect-insect communication using pheromone-based behavioral antagonists.
This paper reports a highly site-selective alkylation of heteroarene N-oxides using hypervalent iodine(III) carboxylates to serve as an alkylating agent in the presence of a cheap copper catalyst ...under visible light conditions. This mild method proceeds at room temperature in an air atmosphere and can withstand various heteroarene N-oxides as well as various primary, secondary, and tertiary alkyl carboxylic acids. It also provides a practical method for enabling the rapid conversion of commercially available raw materials into medically relevant “drug-like” molecules.
Growth patterns of aquatic macrophytes have been shown to vary in response to hydrological properties; however, such properties are typically characterized by water level fluctuation, flow velocity, ...flooding season, and sedimentation, but not by water exchange rate (WER). Herein, we experimentally investigated how WER (three levels: exchange 0%, 20%, and 40% of total water per day) affects water and sediment properties, and the consequences that these variations have on the individual responses of two submerged macrophytes, Hydrilla verticillata and Myriophyllum aquaticum which were planted in two different sediment types (sand and clay). In the experiment without ramets, it was found that turbidity, pH value, and dissolved carbon dioxide concentration of the system water were statistically unaffected by WER, while water dissolved oxygen (DO) concentration and sediment oxidation–reduction potential (ORP, in both sediments) consistently increased with increasing WER, regardless of experimental time. In the experiment containing ramets, biomass accumulation and relative growth rate (RGR) of both species gradually increased with increasing WER regardless of sediment type. The mechanisms were related to (a) increased oxygen availability, as indicated by gradually increased water DO concentration and sediment ORP; and (b) enhanced phosphorus (P) and nitrogen (N) absorbing abilities associated with stimulated root growth, reflected in increased mean root length, specific root length, and the root/above‐ground biomass ratio, with increasing WER. Additionally, in the experiments containing ramets, significant linear relationships were consistently detected between sediment ORP and root parameters, root parameters and plant nutrients (N and P), and plant nutrients and plant growth conditions (biomass accumulation and RGR). These results demonstrate that WER plays an important role in determining oxygen availability and thus impacts the growth of submerged macrophytes by altering the ability of roots to absorb nutrients, indicating that ecosystem functions are more sensitive to WER than previously recognized.
The effect of water exchange rate (WER) on individual characteristics of submerged macrophytes has been investigated. Dissolved oxygen concentration and soil oxidation–reduction potential gradually increased with increasing WER. The growth and elongation of roots were both stimulated with increasing WER. Root’s phosphorus and nitrogen absorbing abilities gradually increased with increasing WER, and thus promoting the growth of aquatic macrophytes.
This paper examines mergers and acquisitions motivated by financial constraints. Synergy gain is measured as the cumulative abnormal return of a value‐weighted portfolio of the acquirer and the ...target around the acquisition announcement. By constructing a financial constraint difference between the target and the acquirer, we find a positive relationship between the financial constraint difference and synergy gains generated from the acquisition. The positive effect of the financial constraint difference is only significant for high growth targets and severely constrained targets. The acquirer's corporate governance also enhances the synergy gains created from the financial constraint difference. Additional evidence shows that both acquirer's and target's shareholders benefit from the financial constraint difference. Our results are robust for different measures of financial constraint.
The interaction between microplastics (MPs) and cadmium (Cd) poses a threat to agricultural soil environments, and their effects on plant growth and rhizosphere microbial community functions are not ...yet clear. In this study, energy sorghum was used as a test plant to investigate the effects of two types of MPs, polystyrene (PS) and polyethylene (PE), at different particle sizes (13 μm, 550 μm) and concentrations (0.1%, 1% w/w), and Cd, as well as their interactions, on the growth of sorghum in a soil-cultivation pot experiment. The results showed that the combined effects of MP and Cd pollution on the dry weight and Cd accumulation rate in sorghum varied depending on the type, concentration, and particle size of the MPs, with an overall trend of increasing stress from combined pollution with increasing Cd content and accumulation. High-throughput sequencing analysis revealed that combined MP and Cd pollution increased bacterial diversity, and the most significant increase was observed in the abundance-based coverage estimator (ACE), Shannon, and Sobs indices in the 13 μm 1% PS+Cd treatment group. Metagenomic analysis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) metabolic pathways revealed that 19 groups of metabolic pathways, including microbial metabolism and methane metabolism, differed significantly under combined MP and Cd pollution. Hierarchical clustering results indicated that Cd treatment and combined MP and Cd treatment affected the abundances of sorghum rhizosphere soil nitrogen (N) and phosphorus (P) cycling genes and that the type of MP present was an important factor affecting N and P cycling genes. The results of this study provide a basis for exploring the toxic effects of combined MP and Cd pollution and for conducting soil environmental risk assessments.
Display omitted
•Combined MP and Cd treatment tended to increase stress in sorghum plants.•Combined MP and Cd treatment increased Cd content and accumulation in sorghum.•Metagenomic analysis was used to determine the effects of combined MP and Cd treatment on rhizosphere microbial functions.•Combined MP and Cd treatment changed rhizosphere bacterial diversity and community composition.•Combined MP and Cd treatment altered microbial functions.
Background:
Intervertebral disc degeneration (IDD), characterized by diverse pathological changes, causes low back pain (LBP). However, prophylactic and delaying treatments for IDD are limited. The ...aim of our study was to investigate the gene network and biomarkers of IDD and suggest potential therapeutic targets.
Methods:
Differentially expressed genes (DEGs) associated with IDD were identified by analyzing the mRNA, miRNA, and lncRNA expression profiles of IDD cases from the Gene Expression Omnibus (GEO). The protein–protein interaction (PPI) network, Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis as well as miRNA–lncRNA–mRNA networks were conducted. Moreover, we obtained 71 hub genes and performed a comprehensive analysis including GO, KEGG, gene set enrichment analysis (GSEA), gene set variation analysis (GSVA), Disease Ontology (DO), methylation analysis, receiver operating characteristic (ROC) curve analysis, immune infiltration analysis, and potential drug identification. We finally used qRT-PCR to verify 13 significant DEGs in normal and degenerative nucleus pulposus cells (NPCs).
Results:
We identified 305 DEGs closely related to IDD. The GO and KEGG analyses indicated that changes in IDD are significantly associated with enrichment of the inflammatory and immune response. GSEA analysis suggested that cell activation involved in the inflammatory immune response amide biosynthetic process was the key for the development of IDD. The GSVA suggested that DNA repair, oxidative phosphorylation, peroxisome, IL-6-JAK-STAT3 signaling, and apoptosis were crucial in the development of IDD. Among the 71 hub genes, the methylation levels of 11 genes were increased in IDD. A total of twenty genes showed a high functional similarity and diagnostic value in IDD. The result of the immune cell infiltration analysis indicated that seven genes were closely related to active natural killer cells. The most relevant targeted hub genes for potential drug or molecular compounds were MET and PIK3CD. Also, qRT-PCR results showed that ARHGAP27, C15orf39, DEPDC1, DHRSX, MGAM, SLC11A1, SMC4, and LINC00887 were significantly downregulated in degenerative NPCs; H19, LINC00685, mir-185-5p, and mir-4306 were upregulated in degenerative NPCs; and the expression level of mir-663a did not change significantly in normal and degenerative NPCs.
Conclusion:
Our findings may provide new insights into the functional characteristics and mechanism of IDD and aid the development of IDD therapeutics.
PDF
