A new alkaloid, identified as (E)-2-(4-hydroxyphenyl)-3-(pyridazin-3-yl)-acrylaldehyde, named oleradazine, was isolated from Portulaca oleracea L., and the structure was elucidated using ...spectroscopic methods, including 1D and 2D NMR spectroscopic and UHPLC-ESI-QTOF/MS methods. In addition, the compound was used to investigate its anti-inflammatory activity in lipopolysaccharide-stimulated macrophages. It was suggested that the oleradazine can significantly inhibit the inflammatory factors, interleukin 1β and nitric oxide in lipopolysaccharide-stimulated RAW 264.7 cells by enzyme-linked immunosorbent assays.
Hypoxic-ischemic injury is a common pathological dysfunction in clinical settings. Mitochondria are sensitive organelles that are readily damaged following ischemia and hypoxia. Dynamin-related ...protein 1 (Drp1) regulates mitochondrial quality and cellular functions via its oligomeric changes and multiple modifications, which plays a role in mediating the induction of multiple organ damage during hypoxic-ischemic injury. However, there is active controversy and gaps in knowledge regarding the modification, protein interaction, and functions of Drp1, which both hinder and promote development of Drp1 as a novel therapeutic target. Here, we summarize recent findings on the oligomeric changes, modification types, and protein interactions of Drp1 in various hypoxic-ischemic diseases, as well as the Drp1-mediated regulation of mitochondrial quality and cell functions following ischemia and hypoxia. Additionally, potential clinical translation prospects for targeting Drp1 are discussed. This review provides new ideas and targets for proactive interventions on multiple organ damage induced by various hypoxic-ischemic diseases.
Brazing technology is widely used in modern industrial systems as an important connection method. The brazing joints are the weakest zone in the whole structure and directly determine the working ...efficiency and life of the entire system. However, the research on the connection mechanism and fracture behavior of brazing joints is still unclear. In this study, the peeling force and displacement curves during the peeling process are tested by using T-type specimens. Based on the cohesive zone model, the peeling energy of each part during the whole peeling process is calculated and analyzed. The results show that the whole peeling process can be divided into three stages, including the initial stage, crack propagation stage, and stable peeling stage. The peeling energy of each stage can be calculated experimentally. The larger the peeling energy, the better the joint performance. Then, a simplified calculation method for peeling energy is developed for T-type joints and is verified as accurate using experimental data. It is also observed that the increase in the base material thickness can effectively improve the peeling performance of the joints. This provides a feasible and effective method for peel strength calculation and evaluation in brazing joints.
Crown removal revitalises sand‐fixing shrubs that show declining vigour with age in drought‐prone environments; however, the underlying mechanisms are poorly understood. Here, we addressed this ...knowledge gap by comparing the growth performance, xylem hydraulics and plant carbon economy across different plant ages (10, 21 and 33 years) and treatments (control and crown removal) using a representative sand‐fixing shrub (Caragana microphylla Lam.) in northern China. We found that growth decline with plant age was accompanied by simultaneous decreases in soil moisture, plant hydraulic efficiency and photosynthetic capacity, suggesting that these interconnected changes in plant water relations and carbon economy were responsible for this decline. Following crown removal, quick resprouting, involving remobilisation of root nonstructural carbohydrate reserves, contributed to the reconstruction of an efficient hydraulic system and improved plant carbon status, but this became less effective in older shrubs. These age‐dependent effects of carbon economy and hydraulics on plant growth vigour provide a mechanistic explanation for the age‐related decline and revitalisation of sand‐fixing shrubs. This understanding is crucial for the development of suitable management strategies for shrub plantations constructed with species having the resprouting ability and contributes to the sustainability of ecological restoration projects in water‐limited sandy lands.
Summary statement
Embolism accumulation restricted carbon assimilation in aging shrubs and resulted in growth decline, while the crown‐removal treatment revitalised plants by promoting the construction of new hydraulic systems in resprouts and enhancing carbon balance through the removal of dysfunctional old stems.
Microstructure, texture, precipitation, mechanical and magnetic properties in a twin-roll strip casting Cu-bearing non-oriented electrical steel were studied by a combination of X-ray diffraction ...(XRD), electron back-scattered diffraction (EBSD), optical microscopy (OM) and transmission electron microscopy (TEM). The results indicated that the average grain size and main texture component were not affected by aging time, while the size and crystal structure of copper precipitates were influenced. In the peak strength condition, copper precipitation contributed ~ 207MPa to the yield strength of the final product without deteriorating the magnetic properties. In summary, a non-oriented electrical steel with excellent mechanical and magnetic properties was successfully processed via twin-roll strip casting.
To predict massive cerebral infarction (MCI) occurrence after anterior circulation occlusion (ACO) by cASPECTS-CTA-CS (combined ASPECTS and CTA-CS). Of 185 cerebral infarction patients with the ACO, ...their collateral circulation scores from CT angiography (CTA) images in two groups (MCI and non-MCI) were evaluated using Alberta Stroke Program Early CT Score (ASPECTS) and CT angiography collateral score (CTA-CS) approaches. The cASPECTS-CTA-CS was validated internally using the bootstrap sampling method with 1000 bootstrap repetitions and compared to CTA-CS. Receiver-operating characteristic curve (ROC), clinical impact curve (CIC), and decision curve analysis (DCA) strategies were used to assess the clinical practicality and predictability of both approaches (cASPECTS-CTA-CS and CTA-CS). Using net reclassification improvement (NRI) and integrated discrimination improvement (IDI) analyses, discrimination levels of the cASPECTS-CTA-CS were compared with CTA-CS. Classification and regression tree (CART) analyses was conducted to identify the best predictive values and identify subgroup of MCI. The discrimination ability of collateral circulation evaluation score using the cASPECTS-CTA-CS AUC: 0.918, 95% confidence interval (CI): 0.869-0.967, P < 0.01; NRI: 0.200, 95% CI: -0.104 to 0.505, P = 0.197; and IDI: 0.107, 95% CI: 0.035-0.178, P = 0.004 was better than CTA-CS alone (AUC: 0.885, 95% CI: 0.833-0.937, P < 0.01). DCA indicated the net benefits of the cASPECTS-CTA-CS approach was higher than CTA-CS alone when the threshold probability range over 20%. CIC analyses showed that the number of high risks and true positives were in agreement when the threshold probability > 80%. Less than 23 of cASPECTS-CTA-CS by CART was important factor in determining MCI occurrence, and ASPECTS < 7 was followed factor. The cASPECTS-CTA-CS approach cumulatively predicted MCI after ACO.
To analyse the value of the apparent diffusion coefficient (ADC) in diffusion-weighted imaging (DWI) and the choline (Cho)/creatine (Cr) ratio and Cho/N-acetyl-aspartate (NAA) ratio in magnetic ...resonance spectroscopy (MRS) in the differential diagnosis between recurrent glioma and radiation injury. Chinese and English studies related to the diagnosis of recurrent glioma and radiation injury using DWI and MRS and published before 15 October 2022 were retrieved from PubMed, Embase, the Cochrane Library, China National Knowledge Infrastructure, China Biomedical Literature Database, VIP Journal Database, and Wanfang Database for a meta-analysis. A total of 11 articles were included in this study. ADC was lower in the recurrent glioma group than in the radiation injury group (standardized mean difference = −1.29, 95% confidence interval (CI) (−1.87, −0.71), P<0.001). The Cho/Cr ratio was higher in the recurrent glioma group than in the radiation injury group (weighted mean difference = 0.65, 95% CI (0.40, 0.90), and P<0.001). The Cho/NAA ratio was higher in the recurrent glioma group than in the radiation injury group, as evidenced by the sensitivity analysis. The sensitivity and specificity of the Cho/Cr ratio were 0.85 (0.73–0.92) and 0.82 (0.67–0.91), respectively, and the area under the curve was 0.86. The sensitivity and specificity of the Cho/NAA ratio were 0.82 (0.66–0.91) and 0.94 (0.69–0.99), respectively, and the area under the curve was 0.93. This meta-analysis showed that ADC, Cho/Cr, and Cho/NAA ratios all had high sensitivity and specificity. Therefore, DWI combined with MRS can effectively improve the diagnosis of recurrent glioma and radiation injury.
Few studies focused on the roles of high glucose combined with high lipid in placental development or fetal growth. This study was designed to investigate the roles of high glucose combined with high ...lipid in mitochondrial dysfunction of JEG-3 cells. We determined the cellular proliferation and apoptosis, superoxide dismutase (SOD) activity, concentration of malondialdehyde (MDA), and lactic acid dehydrogenase in control group, high glucose group, high lipid group, and high glucose and high lipid group, together with the mitochondrial dysfunction, Nrf2, HO-1, SMAC, and cytochrome
(Cyt-
) expression. Significant decrease of SOD and significant elevation of MDA was seen in high glucose and high lipid group compared with the other three groups. There was significant decrease in mitochondrial SMAC and Cyt-
in high glucose group, high lipid group, and high glucose and high lipid group compared with those of control group. Nrf2 and HO-1 protein expression in high glucose combined with high lipid group showed significant decrease compared with that of high lipid group or high glucose group. We speculated that combination of high glucose and high lipid induced oxidative stress in JEG-3 cells, and Nrf2/ARE pathway may be related to this process.
We report an unprecedented C–H bond functionalization of cyclopropenes enabled by dinuclear gold catalysis. Highly selective C–H allylation, alkynylation and halogenation of cyclopropenes with ...organic halides have been realized. The reaction does not require strong external oxidants and affords access to functionalized cyclopropenes in moderate to good yields. The reductive elimination process to controllably construct C–C or C–X bonds can be tuned by using different dinuclear gold catalysts.
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