In this paper, we present an experiment- and simulation-based study to evaluate the use of full duplex (FD) as a potential mode in practical IEEE 802.11 networks. To enable the study, we designed a ...20-MHz multiantenna orthogonal frequency-division-multiplexing (OFDM) FD physical layer and an FD media access control (MAC) protocol, which is backward compatible with current 802.11. Our extensive over-the-air experiments, simulations, and analysis demonstrate the following two results. First, the use of multiple antennas at the physical layer leads to a higher ergodic throughput than its hardware-equivalent multiantenna half-duplex (HD) counterparts for SNRs above the median SNR encountered in practical WiFi deployments. Second, the proposed MAC translates the physical layer rate gain into near doubling of throughput for multinode single-AP networks. The two results allow us to conclude that there are potentially significant benefits gained from including an FD mode in future WiFi standards.
Triple-negative breast cancer (TNBC) is a heterogeneous and clinically aggressive disease for which there is no targeted therapy. BET bromodomain inhibitors, which have shown efficacy in several ...models of cancer, have not been evaluated in TNBC. These inhibitors displace BET bromodomain proteins such as BRD4 from chromatin by competing with their acetyl-lysine recognition modules, leading to inhibition of oncogenic transcriptional programs. Here we report the preferential sensitivity of TNBCs to BET bromodomain inhibition in vitro and in vivo, establishing a rationale for clinical investigation and further motivation to understand mechanisms of resistance. In paired cell lines selected for acquired resistance to BET inhibition from previously sensitive TNBCs, we failed to identify gatekeeper mutations, new driver events or drug pump activation. BET-resistant TNBC cells remain dependent on wild-type BRD4, which supports transcription and cell proliferation in a bromodomain-independent manner. Proteomic studies of resistant TNBC identify strong association with MED1 and hyper-phosphorylation of BRD4 attributable to decreased activity of PP2A, identified here as a principal BRD4 serine phosphatase. Together, these studies provide a rationale for BET inhibition in TNBC and present mechanism-based combination strategies to anticipate clinical drug resistance.
The use of directional antennas in wireless networks has been widely studied with two main motivations: 1) decreasing interference between devices and 2) improving power efficiency. We identify a ...third motivation for utilizing directional antennas: pushing the range limitations of full-duplex wireless communication. A characterization of full-duplex performance in the context of a base station transmitting to one device while receiving from another is presented. In this scenario, the base station can exploit "directional diversity" by using directional antennas to achieve additional passive suppression of the self-interference. The characterization shows that at 10 m distance and with 12 dBm transmit power the gains over half-duplex are as high as 90% and no lower than 60% as long as the directional antennas at the base station are separated by 45° or more. At 15 m distance the gains are no lower than 40% for separations of 90° and larger. Passive suppression via directional antennas also allows full-duplex to achieve significant gains over half-duplex even without resorting to the use of extra hardware for performing RF cancellation as has been required in the previous work.
Tuberous sclerosis complex (TSC) is an autosomal dominant tumor suppressor syndrome, characterized by tumor development in multiple organs, including renal angiomyolipoma. Biallelic loss of TSC1 or ...TSC2 is a known genetic driver of angiomyolipoma development, however, whether an altered transcriptional repertoire contributes to TSC-associated tumorigenesis is unknown. RNA-seq analyses showed that MITF A isoform (MITF-A) was consistently highly expressed in angiomyolipoma, immunohistochemistry showed microphthalmia-associated transcription factor nuclear localization, and Chromatin immuno-Precipitation Sequencing analysis showed that the MITF-A transcriptional start site was highly enriched with H3K27ac marks. Using the angiomyolipoma cell line 621-101, MITF knockout (MITF.KO) and MITF-A overexpressing (MITF.OE) cell lines were generated. MITF.KO cells showed markedly reduced growth and invasion in vitro, and were unable to form xenografted tumors. In contrast, MITF.OE cells grew faster in vitro and as xenografted tumors compared to control cells. RNA-Seq analysis showed that both ID2 and Cysteine-rich angiogenic inducer 61 (CYR61) expression levels were increased in the MITF.OE cells and reduced in the MITF.KO cells, and luciferase assays showed this was due to transcriptional effects. Importantly, CYR61 overexpression rescued MITF.KO cell growth in vitro and tumor growth in vivo. These findings suggest that MITF-A is a transcriptional oncogenic driver of angiomyolipoma tumor development, acting through regulation of CYR61.
Extensive cross-linking introduced during routine tissue fixation of clinical pathology specimens severely hampers chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) ...analysis from archived tissue samples. This limits the ability to study the epigenomes of valuable, clinically annotated tissue resources. Here we describe fixed-tissue chromatin immunoprecipitation sequencing (FiT-seq), a method that enables reliable extraction of soluble chromatin from formalin-fixed paraffin-embedded (FFPE) tissue samples for accurate detection of histone marks. We demonstrate that FiT-seq data from FFPE specimens are concordant with ChIP-seq data from fresh-frozen samples of the same tumors. By using multiple histone marks, we generate chromatin-state maps and identify cis-regulatory elements in clinical samples from various tumor types that can readily allow us to distinguish between cancers by the tissue of origin. Tumor-specific enhancers and superenhancers that are elucidated by FiT-seq analysis correlate with known oncogenic drivers in different tissues and can assist in the understanding of how chromatin states affect gene regulation.
Atopic dermatitis (AD) is a chronic inflammatory skin disease accompanied by severe itching and dry skin. Currently, the incidence of AD due to excessive activation of immune cells by various ...environmental factors is increasing worldwide, and research on inflammatory response inhibitors with fewer side effects is continuously needed. Cynanoside F (CF) is one of the pregnane-type compounds in the root of Cynanchum atratum, an oriental medicinal herb that has been shown to have antioxidant, antitumor, and anti-inflammatory effects. Although CF has been isolated as a component in Cynanchum atratum, the scientific role of CF has not yet been explored. In this study, we evaluated the effect of CF on AD and revealed the mechanism using in vitro and in vivo experimental models. CF significantly reduced lipopolysaccharide (LPS)-induced protein expression levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), and cyclooxygenase-2 (COX-2), which are important proinflammatory mediators in the RAW264.7 macrophage cell line. CF did not inhibit the nuclear factor-kappa B (NF-κB) signaling activated by LPS but significantly reduced the phosphorylation of mitogen-activated protein kinases (MAPKs), such as p38 MAPK, JNK, and ERK. CF consistently inhibited the activity of the activator protein-1 (AP-1) transcription factor, a downstream molecule of MAPK signaling. In addition, in an experiment using an oxazolone-induced AD mouse model, the CF-treated group showed a marked decrease in epidermal thickness, the number of infiltrated mast cells, and the amount of histamine. The mRNA levels of IL-1β, interleukin-4 (IL-4), and thymic stromal lymphopoietin (TSLP) were consistently lowered in the group treated with CF. Moreover, the phosphorylation of c-Jun and c-Fos protein levels, which are the AP-1 components, were lowered in the skin tissues of CF-treated mice. These results provide the first evidence that CF has an inhibitory effect on AD and suggest the possibility of CF being developed as a potential therapeutic agent for AD.
Pesticide use affects biodiversity and ecosystem service provision. Yet, such effects may vary between pesticides, dosage, application timing and pollinator assemblage in the study area. ...Understanding such diversity of effects is essential to enable integrated pest management practices that minimize negative impacts on pollinators. Here, we use a controlled experiment to compare different strategies of pesticide application in tomato crops: no application, low‐intensity applications (every 7 days) and high‐intensity application (every 3–4 days) with the last being the usual practice used by farmers in the study region. We focus on imidacloprid that is a neonicotinoid insecticide commonly used in Brazil. We show the negative effects of imidacloprid on the pollinator visitation rate of tomato flowers varied between flower visitor species. While the bee Paratrigona lineata was negatively affected by the imidacloprid application, no effects were detected when analysing all other bees as a group, indicating a null net effect. Although some studies have shown this insecticide is extremely harmful to bees' health, others showed that some bees do not avoid it, which may explain our results. However, the visitation rate recovery for P. lineata was only detected in the less than the more intensive treatment after imidacloprid applications ceased. The fact that no differences in fruit production were detected between treatments (including control blocks) could result from a null net effect of the negative effects on pollinators and positive effects on pest control. Further studies would be needed to disentangle the two effects.
On Full-Duplex Radios With Modulo-ADCs Ordonez, Luis G.; Ferrand, Paul; Duarte, Melissa ...
IEEE open journal of the Communications Society,
2021, Letnik:
2
Journal Article
Recenzirano
Odprti dostop
Wireless full-duplex (FD) transceivers have become more commonplace in the recent years, enabled by improvements in hardware as well as in signal processing algorithms dedicated to self-interference ...(SI) mitigation. A key design target for any FD radio is to limit the analog-to-digital converter (ADC) quantization noise affecting the signal of interest (SoI), which results from the difference in dynamic range between the SI and the SoI at the input of the ADC. Indeed, when using conventional ADCs, since the SI power is much larger than that of the SoI, the SI spans most of the ADC's dynamic range and the SoI becomes distorted by a large quantization noise. Reducing the power of the SI before the ADC, for example via analog domain SI cancellation, is so far the only means to mitigate this undesired effect. In this paper we consider the use of so-called modulo ADCs as a new tool to reduce the quantization noise that affects the SoI in the presence of SI. We demonstrate theoretically and numerically that by substituting the conventional ADCs with modulo-ADCs, which fold the analog input signal before analog-to-digital conversion, and by using appropriately designed analog gain control and digital-domain SI cancellation, one can reduce the quantization noise that affects the SoI. As such, the work in this paper provides a new technique for counteracting the detrimental effect of SI in analog-to-digital conversion and, thus, provides a new route to be explored in order to enhance the performance of FD radios.
We present an experiment-based characterization of passive suppression and active self-interference cancellation mechanisms in full-duplex wireless communication systems. In particular, we consider ...passive suppression due to antenna separation at the same node, and active cancellation in analog and/or digital domain. First, we show that the average amount of cancellation increases for active cancellation techniques as the received self-interference power increases. Our characterization of the average cancellation as a function of the self-interference power allows us to show that for a constant signal-to-interference ratio at the receiver antenna (before any active cancellation is applied), the rate of a full-duplex link increases as the self-interference power increases. Second, we show that applying digital cancellation after analog cancellation can sometimes increase the self-interference, and thus digital cancellation is more effective when applied selectively based on measured suppression values. Third, we complete our study of the impact of self-interference cancellation mechanisms by characterizing the probability distribution of the self-interference channel before and after cancellation.
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