Diabetic nephropathy and inflammation Montserrat B Duran-Salgado Alberto F Rubio-Guerra
World journal of diabetes,
06/2014, Letnik:
5, Številka:
3
Journal Article
Odprti dostop
Diabetic nephropathy(DN) is the leading cause of end-stage renal failure worldwide. Besides, diabetic nephropathy is associated with cardiovascular disease, and increases mortality of diabetic ...patients. Several factors are involved in the pathophysiology of DN, including metabolic and hemodynamic alterations, oxidative stress, and activation of the renin-angiotensin system. In recent years, new pathways involved in the development and progression of diabetic kidney disease have been elucidated; accumulated data have emphasized the critical role of inflammation in the pathogenesis of diabetic nephropathy. Expression of cell adhesion molecules, growth factors, chemokines and pro-inflammatory cytokines are increased in the renal tissues of diabetic patients, and serum and urinary levels of cytokines and cell adhesion molecules, correlated with albuminuria. In this paper we review the role of inflammation in the development of diabetic nephropathy, discussing some of the major inflammatory cytokines involved in the pathogenesis of diabetic nephropathy, including the role of adipokines, and take part in other mediators of inflammation, as adhesion molecules.
An electrochemical impedimetric immunosensor was developed for the detection of the neuropeptide Corticotropin Releasing Hormone (CRH) based on the immobilization of half-antibody fragments on gold ...nanoparticles (AuNp). Then, the optimal conditions for the obtainment of AuNp through electroplating on a bare gold electrode were studied. The results showed that the obtainment of AuNp at a fixed potential of −0.2 V for 330 s, at 80 °C and 2·10-3 mol·L-1 of HAuCl4 generates an adequate nanostructured surface and is a highly reproducible method. Also, the optimal conditions for immobilizing the half-antibody on AuNp were studied. The interaction of the CRH with the recognition layer of the immobilized half-antibody on the nanostructured surface was carried out by incubation at 4 °C for 2 h. A dissolution of Fe(CN)64-/Fe(CN)63− as a redox probe was used to study the electrochemical responses of the nanostructured surface and the immobilization processes of the half-antibody and detection of CRH, using cyclic voltammetry and electrochemical impedance spectroscopy. An immunosensor was obtained for the specific detection of CRH, within a range of 10.0–80.0 μg mL-1, with a limit of detection of 2.7 μg mL-1 and a limit of quantification of 9.2 μg mL-1. Additionally, the association constant between the CRH and the immobilized half-antibody was calculated at 1.96·105 M−1.
•First electrochemical immunosensor developed for Corticotropin Releasing Hormone.•The adequate electrochemical surface was obtained under optimal condition experimentals.•Electrochemical impedance spectroscopy was used for the electrochemical determination.•The modified electrode possesses good specificity and stability.
Notch signaling is central to vertebrate development, and analysis of Notch has provided important insights into pathogenetic mechanisms in the CNS and many other tissues. However, surprisingly ...little is known about the role of Notch in the development and pathology of Schwann cells and peripheral nerves. Using transgenic mice and cell cultures, we found that Notch has complex and extensive regulatory functions in Schwann cells. Notch promoted the generation of Schwann cells from Schwann cell precursors and regulated the size of the Schwann cell pool by controlling proliferation. Notch inhibited myelination, establishing that myelination is subject to negative transcriptional regulation that opposes forward drives such as Krox20. Notably, in the adult, Notch dysregulation resulted in demyelination; this finding identifies a signaling pathway that induces myelin breakdown in vivo. These findings are relevant for understanding the molecular mechanisms that control Schwann cell plasticity and underlie nerve pathology, including demyelinating neuropathies and tumorigenesis.
The proton is one of the main building blocks of all visible matter in the Universe
. Among its intrinsic properties are its electric charge, mass and spin
. These properties emerge from the complex ...dynamics of its fundamental constituents-quarks and gluons-described by the theory of quantum chromodynamics
. The electric charge and spin of protons, which are shared among the quarks, have been investigated previously using electron scattering
. An example is the highly precise measurement of the electric charge radius of the proton
. By contrast, little is known about the inner mass density of the proton, which is dominated by the energy carried by gluons. Gluons are hard to access using electron scattering because they do not carry an electromagnetic charge. Here we investigated the gravitational density of gluons using a small colour dipole, through the threshold photoproduction of the J/ψ particle. We determined the gluonic gravitational form factors of the proton
from our measurement. We used a variety of models
and determined, in all cases, a mass radius that is notably smaller than the electric charge radius. In some, but not all cases, depending on the model, the determined radius agrees well with first-principle predictions from lattice quantum chromodynamics
. This work paves the way for a deeper understanding of the salient role of gluons in providing gravitational mass to visible matter.
The aim of the present study was to evaluate: 1) the association between AMH, AFC, superovulatory response and embryo yield in sheep; and 2) the effect of FSH treatment length during superstimulation ...of the first follicular wave on ovarian response and embryo yield, particularly in ewes with low and high AMH. The experiment was performed on 63 Polled Dorset ewes that received an ovarian superstimulatory treatment during the first follicular wave (Day 0 protocol). Ewes were administered a total dose of 240 mg of FSH distributed in six (6-dose regimen, n = 30) or eight (8-dose regimen, n = 33) decreasing doses administered 12 h apart. On Day −9 (random stage of the estrous cycle) and Day 0 (day of the first FSH dose) ovarian ultrasonography was performed and blood samples were collected for AFC and AMH determinations, respectively. A weak positive correlation between AMH and small AFC (follicles <4 mm) was observed (r = 0.23; P = 0.07), and AMH concentration was positively correlated (r = 0.29; P < 0.05) with the number of corpora lutea (CL) determined at embryo collection (i.e., 6 d after insemination). The length of FSH treatment tended (P = 0.06) to affect the ovarian response, such that the number of CL was greater in 8-dose than 6-dose treated ewes, while no differences (P > 0.10) in embryo yield outcomes were observed. For further analysis, ewes were classified into low (<7 ng/mL) and high (>10 ng/mL) serum AMH. In high AMH ewes, there were no differences (P > 0.05) in the number of CL nor embryo yield between the 6-dose and 8-dose treatment (e.g., 7.8 ± 2.4 and 8.3 ± 2.5 transferable embryos, respectively; P = 0.92). Conversely, for low AMH ewes, fertilized ova and embryo yield were greater (P ≤ 0.05) for ewes receiving the 8-dose than the 6-dose superstimulatory treatment (e.g., 8.4 ± 2.8 vs. 2.7 ± 0.9 transferable embryos, respectively, P ≤ 0.05). In conclusion, embryo production in poor responding ewes with low low circulating AMH is improved by extending the superstimulatory treatment length from 6 to 8 FSH doses.
•AMH is weakly correlated with AFC and ovarian response after superstimulation.•Superovulatory response is not affect by superstimulatory treatment length.•Lengthening the FSH treatment improves embryo yield in low but not high AMH ewes.
Herein, we report the surface modification of H2O2-treated nanoporous alumina (anodic aluminum oxide, AAO) membranes with chitosan (CS) by a solution casting method. Later, copper nanoparticles were ...incorporated into this CS-AAO membrane by immersion in an aqueous solution of a copper salt precursor and subsequent reduction using NaBH4. The IR spectra and energy dispersive X-ray spectroscopy (EDS) analyses helped to confirm the presence of CS and copper nanoparticles both on the surface and inside the nanochannels of this AAO membrane (denoted CCSA). Notable differences in the surface energy, surface topography and morphology were observed between the CS-AAO and CCSA membranes. The catalytic performance of the CCSA membrane was tested in the reduction of methylene blue (MB).Our findings revealed that this system adequately combines the attractive properties of supporting of the AAO membranes, ion retention by CS and the catalytic activity of copper nanoparticles into a tridimensional array. Finally, the recyclability of the CCSA membrane was also assessed. The results showed that the performance of this membrane was not dramatically affected after four cycles of use.
Nonnative species are often perceived to cause the decline or impede management and recovery of native species, yet the ability to quantify the ecological impacts of nonnative species is often ...difficult. Disentangling the consequences of other stressors (e.g., habitat loss, climate change) and nonnative invasions on native fish communities might be accomplished by a thorough understanding of spatial and temporal variation in nonnative fish behavior and physiological rates of prey consumption mediated by environmental conditions. We examined the potential predatory and competitive threat of nonnative channel catfish (
Ictalurus punctatus
) on a native fish community in the San Juan River, New Mexico, Colorado, and Utah by examining how diet composition and prey intake varied across diel, seasonal, and annual temporal scales over a broad spatial extent (152 km). Prey intake rate and diet composition did not differ across diel temporal scales, but shifted across seasonal and annual scales as well as longitudinally. Estimated consumption of prey by the entire channel catfish population varied between 9.3 dry kg/ha/yr in 2019 and 16.5 dry kg/ha/yr in 2018 with consumption dominated by nonnative terrestrial plants (57.9%; mainly nonnative Russian olive seeds), aquatic invertebrates (13.4%), and aquatic plants (9.0%). The probability of prey in the diet of channel catfish was largely predicted by factors influencing their availability in the system, highlighting the opportunistic feeding strategy of channel catfish. Native fish were consistent in the diet spatially but increased with higher water turbidity and in larger catfish, and despite their relatively low proportion of total prey consumed (2.6%) the impact of this number depends on population sizes of the nonnative predator and native prey. Opportunistic feeding across a wide range of prey items likely allows catfish to sustain high abundances in this system, increasing the potential for negative interaction with native fishes. However, this foraging strategy is dominated by nonnative Russian olive seeds, which may also reduce predatory and competitive interactions with native fishes.
The visible world is founded on the proton, the only composite building block of matter that is stable in nature. Consequently, understanding the formation of matter relies on explaining the dynamics ...and the properties of the proton's bound state. A fundamental property of the proton involves the response of the system to an external electromagnetic field. It is characterized by the electromagnetic polarizabilities
that describe how easily the charge and magnetization distributions inside the system are distorted by the electromagnetic field. Moreover, the generalized polarizabilities
map out the resulting deformation of the densities in a proton subject to an electromagnetic field. They disclose essential information about the underlying system dynamics and provide a key for decoding the proton structure in terms of the theory of the strong interaction that binds its elementary quark and gluon constituents. Of particular interest is a puzzle in the electric generalized polarizability of the proton that remains unresolved for two decades
. Here we report measurements of the proton's electromagnetic generalized polarizabilities at low four-momentum transfer squared. We show evidence of an anomaly to the behaviour of the proton's electric generalized polarizability that contradicts the predictions of nuclear theory and derive its signature in the spatial distribution of the induced polarization in the proton. The reported measurements suggest the presence of a new, not-yet-understood dynamical mechanism in the proton and present notable challenges to the nuclear theory.
The CLAS12 Geant4 simulation Ungaro, M.; Angelini, G.; Battaglieri, M. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
04/2020, Letnik:
959, Številka:
C
Journal Article
Recenzirano
Odprti dostop
The Geant4 Monte-Carlo (GEMC) package is used to simulate the passage of particles through the various CLAS12 detectors. The geometry is implemented through a database of Geant4 volumes created ...either through the GEMC native API, by the CLAS12 geometry service, or imported from the CAD engineering model. The truth information is digitized with a plugin mechanism by routines specific to each detector and includes the use of the CLAS12 calibration database constants to produce both ADC and TDC response functions. Theoretical models that produce the generated events interface with GEMC through the LUND data format. The merging of simulated data with real random trigger data provides a mechanism to include both beam and electronic background into the simulation of generated events to accurately model beam data from the CLAS12 detector. The performance of simulation is demonstrated by comparison with the experimental data.
PDF
