Foetal membranes are essential tissues for embryonic development, playing important roles related to protection, breathing, nutrition and excretion. The amnion is the innermost extraembryonic ...membrane, which surrounds the foetus, forming an amniotic sac that contains the amniotic fluid (AF). In recent years, the amniotic membrane has emerged as a potential tool for clinical applications and has been primarily used in medicine in order to stimulate the healing of skin and corneal diseases. It has also been used in vaginal reconstructive surgery, repair of abdominal hernia, prevention of surgical adhesions and pericardium closure. More recently, it has been used in regenerative medicine because the amniotic‐derived stem cells as well as AF‐derived cells exhibit cellular plasticity, angiogenic, cytoprotective, immunosuppressive properties, antitumoural potential and the ability to generate induced pluripotent stem cells. These features make them a promising source of stem cells for cell therapy and tissue engineering. In this review, we discussed the development of the amnion, AF and amniotic cavity in different species, as well as the applicability of stem cells from the amnion and AF in cellular therapy.
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A placenta is defined as structural approximation of maternal and foetal tissues to perform physiological exchange. Associated processes of differentiation and the establishment of its cells ...take place within the extracellular matrix (ECM) that provides a rich environment of collagens, fibronectins, cytokines and other components. Placental ECM is promising for tissue regeneration purposes, because it has immune tolerance capacities that may cause only minimal rejections of transplants with immunological differences between donor and recipient. However, specific characteristics of ECM during evolution of the structurally very diverse mammalian placenta are not yet revealed. We here address the major aspects of placental types, that is non‐invasive (epitheliochorial), medium (endotheliochorial)‐to‐high (haemochorial) invasive nature of the interhemal barrier between the foetal and maternal blood system as well as their main components of ECM with special reference to species that are commonly used as animal models for human placentation and in the potential applications for regenerative medicine.
Abstract Objectives Reproduction in the plains viscacha is characterized by the polyovulation of hundreds of oocytes, the loss of implantation and the development of 1–3 offspring. Our goal was to ...determine whether placental development was affected by these specializations. Study design Thirteen placentas from early pregnancy to near-term pregnancy were analyzed using histological, immunohistochemical and transmission electron microscopy. Results An inverted, villous yolk sac was present. Placentas were formed by the trophospongium, labyrinth and subplacenta. A lobulated structure with a hemomonochorial barrier was established early in pregnancy. Proliferating trophoblast that was clustered at the outer border and inside the labyrinth was responsible for placental growth. Trophoblast invasion resulted from the cellular trophoblast and syncytial streamers derived from the subplacenta. Different from other caviomorphs, numerous giant cells were observed. Conclusions The principle processes of placentation in caviomorphs follow an extraordinarily stable pattern that is independent of specializations, such as polyovulation.
Abstract Caviomorph development includes an inverted yolk sac. Since principle processes are not understood, we investigated its differentiation in Galea and re-examined material from the guinea pig. ...Galea showed the typical caviomorph conditions in blastocyst development and the nature of the definitive yolk sac, formed of the visceral layer that became villous, proliferative, vascularized and attached to the uterus and placenta. In contrast to what was known before, in both species parts of the parietal yolk sac and a yolk sac cavity were temporarily present. Data suggest that early yolk sac development in caviomorphs is more complex than thought before.
Abstract We studied the development of the inverted yolk sac in a New World rodent, Necromys lasiurus during early placentation. Ten implantation sites were investigated by means of histology, ...immunohistochemistry and electron microscopy. The yolk sac was villous near its attachment to the placenta. Elsewhere it was non-villous and closely attached to the uterus. The uterine glands were shallow and wide mouthed. They were associated with vessels and filled with secretion, suggesting the release of histotroph. This feature was absent at later stages. The intimate association of the yolk sac with specialized glandular regions of the uterus may represent a derived character condition of Necromys and/or sigmodont rodents.
Progress in the design and construction of SPES at INFN-LNL Bisoffi, G.; Prete, G.; Andrighetto, A. ...
Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms,
06/2016, Letnik:
376
Journal Article
Recenzirano
Odprti dostop
INFN-LNL is constructing an ISOL (Isotope Separation On Line) facility delivering neutron rich ion beams at 10AMeV or beyond, making use of the linear accelerator ALPI as the secondary accelerator. ...The facility includes a direct ISOL target based on UCx and able to reach 1013fissions/s. In parallel, an applied physics facility will be developed, with applications in medicine and neutron production. The SPES project is a national facility, approved and funded. Commissioning with the first exotic species is expected in 2019. The primary accelerator is a commercial cyclotron, which will send a 40MeV, 200μA proton beam onto an UCx target, connected to SIS, PIS and LIS ion sources. The extracted beam is purified through a Low Resolution Mass Separator (LMRS, i.e. a Wien filter and a dispersive dipole), a beam cooler and a High Resolution Mass Separator (HRMS) and sent to an ECR charge breeder to boost the exotic beam charge state. The highly charged exotic beam is further separated in a MRMS (Medium Resolution Mass Separator) and injected into a 100% duty cycle RFQ and into the existing superconducting linac ALPI, which will be refurbished and upgraded to be an efficient exotic beam accelerator. The upgrade of ALPI will give ∼10AMeV energy to 132Sn19+, taken as the reference ion beam. The paper presents the status of the design and construction of the SPES facility.
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Dogs have been studied for several reasons, such as the genetic improvement, their use as experimental models, in zoonotic research, cell therapy and as a model for human diseases. However, ...many features relating to the embryonic development of dogs remain unknown because of the absence of embryological studies. Considering the importance of the cardiorespiratory system in the development of embryos, the aim of this study was to investigate the development of the main cardiorespiratory organs of dog embryos and foetuses with estimated gestational ages from 16 to 46 days using macro‐ and microscopic descriptions. On day 16 of development, the neural tube and crest were formed, the anterior and posterior neuropore closure had begun and the somites had developed. Between days 22 and 27 of gestation, the lung buds and the initial formation of the primary bronchi and heart chambers were observed. The heart chambers exhibited the endo‐, myo‐ and epicardial layers but did not have obvious differences in thickness among each other. Between days 41 and 46 of gestation, the nasal conchae and septa and trachea were formed, which exhibited characteristic epithelia. The lung formation and lobation were complete. The heart and major vessels exhibited mature histological architecture when their anatomical development was complete. The results of this study contribute to a more accurate definition of the embryonic and foetal developmental stages in dogs.
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Many researches describe the embryonic developmental features in domestic animals; however, in farm animals, they are scarce. Most farm animal studies are related to assisted reproduction ...and embryos transfer techniques. But, morphological features and size measure to estimate the age gestation are rarely reported in literature. Thus, in this study, we described the developmental changes in the bubaline (Bubalus bubali) concepts from 21 to 60 days of gestation. Our results revealed that buffalo embryos similar morphological characteristics similar to other mammalian species. Also, similarities between bovine and bubaline persist; except on foetal stages when buffalos have a faster development than bovine. Therefore, buffalo's gestation period exhibits some varieties and accurate embryo age is more difficult. Yet, when we use a combination of the crown–rump, macroscopic analysis and alizarin red, it is possible to describe better the whole embryogenesis stages of the buffalo and which can contribute for future reproduction researches and applications in veterinary practice.
Status of the SPES Exotic Beam Facility Comunian, M; Andrighetto, A; Antonini, P ...
Journal of physics. Conference series,
01/2020, Letnik:
1401, Številka:
1
Journal Article
Recenzirano
Odprti dostop
At Legnaro National Laboratories of INFN is under construction a Rare Isotope Facility called "Selective Production of Exotic Species" (SPES) based on a 35-70 MeV proton cyclotron, able to deliver ...two beams with a total current up to 0.75 mA, an ISOL fission target station and an existing ALPI superconducting accelerator as a post accelerator (up to 10 MeV/u for A/q=7). The paper will cover notably: the high-resolution mass separator, the CW RFQ (80 MHz, 727 keV/u, with internal bunching), the 1+ low energy transfer line and the injection line from Charge Breeder to ALPI under installation.
SPES (Selective Production of Exotic Species) is an ISOL type facility for production and post-acceleration of exotic nuclei for forefront research in nuclear physics. Radioactive (RA) species (A = ...80÷160) will be produced by fissions induced by a proton beam impinging on an UCx target: the proton beam will be delivered by a commercial cyclotron with a 40 MeV maximum energy and a 0.25 mA maximum current. The RA species, extracted from the Target-Ion-Source system as a 1+ beam, will be cooled in a RFQ (radiofrequency quadrupole) beam cooler (RFQ-BC) and purified from the isobars contaminants through a High Resolution Mass Separator (HRMS). Post-acceleration will be performed via an ECR-based charge breeder, delivering the obtained q+ RA beam to a being built CW RFQ and to the being upgraded superconducting (sc) linac ALPI (up to 10 MeV/A for a mass-to-charge ratio A/q = 7).
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