There is shortage of extensive clinicopathologic studies of cellular senescence because the most reliable senescence biomarker, the detection of Senescence-Associated-beta-galactosidase activity ...(SA-β-gal), is inapplicable in archival material and requires snap-frozen tissues. We validated the histochemical Sudan-Black-B (SBB) specific stain of lipofuscin, an aggregate of oxidized proteins, lipids and metals, known to accumulate in aged tissues, as an additional reliable approach to detect senescent cells independently of sample preparation. We analyzed cellular systems in which senescence was triggered by replicative exhaustion or stressful stimuli, conditional knock-in mice producing precancerous lesions exhibiting senescence, and human preneoplastic lesions known to contain senescent cells. In the above settings we demonstrated co-localization of lipofuscin and SA-β-gal in senescent cells in vitro and in vivo (cryo-preserved tissue), strongly supporting the candidacy of lipofuscin for a biomarker of cellular senescence. Furthermore, cryo-preserved tissues positive for SA-β-gal were formalin-fixed, paraffin-embedded, and stained with SBB. The corresponding SA-β-gal positive tissue areas stained specifically for lipofuscin by SBB, whereas tissues negative for SA-β-gal were lipofuscin negative, validating the sensitivity and specificity of the SBB staining to visualize senescent cells in archival material. The latter unique property of SBB could be exploited in research on widely available retrospective tissue material.
Live fuel moisture content (LFMC) is an important factor in fire risk management in the Mediterranean region. Drawing upon a large network of stations (the Réseau Hydrique) measuring LFMC for ...operational fire danger assessment in the south-eastern region of France, this study assesses the ability of several long-term passive microwave remote sensing indices to capture the LFMC temporal dynamic of various Mediterranean shrub species. Microwave remote sensing has a high potential for monitoring LFMC independently of several constraints (e.g., atmospheric and cloud contamination effects) associated with optical-infrared and thermal remote sensing observations. The following four microwave-derived indices are considered: (1) the Essential Climate Variable near-surface soil moisture (ECV_SM); (2) the root-zone soil moisture (ECV_RZSM) derived from ECV_SM; (3) the microwave polarization difference index (MPDI) computed from five microwave frequencies (C, X, Ku, K and Ka-band corresponding to 6.9, 10.7, 18.7, 23.8 and 36.5GHz respectively); and (4) the vegetation optical depth (VOD) at C- and X-band (from the Advanced Microwave Scanning Radiometer for the Earth observing system, AMSR-E). Firstly, an evaluation of the root-zone soil moisture ECV_RZSM against a network of soil moisture measurements (SMOSMANIA in southern France) gave satisfactory results. For most of the Réseau Hydrique sites, the present study found good agreement between LFMC and individual microwave indices, including root-zone soil moisture, VOD at X-band, and MPDI at X and Ku-bands, all averaged over the 15days preceding the in-situ LFMC measurements. VOD at X-band showed the best agreement with the in situ LFMC data (median of correlation coefficients over all in situ sites=0.43). Further comparisons between LFMC data and several optical indices computed from the Moderate Resolution Imaging Spectrometer (MODIS) data including normalized difference vegetation index (NDVI), soil adjusted vegetation index (SAVI), visible atmospheric resistant index (VARI), normalized difference water index (NDWI), normalized difference infrared index 6 (NDII6), normalized difference infrared index 7 (NDII7) and global vegetation moisture index (GVMI) were made. The comparisons showed that VARI and SAVI, as optical greenness indices, outperform the microwave indices and other optical indices with median of correlation coefficients of 0.66 and 0.65, respectively. Overall, this study shows that passive microwave indices, particularly VOD, are efficient proxies for LFMC of Mediterranean shrub species and could be used along with optical indices to evaluate fire risks in the Mediterranean region.
•Live fuel moisture content (LFMC) is an important parameter in fire risk management.•We evaluated microwaves & optical remote sensing indices for monitoring LFMC.•The Root Zone SM product was based on the new ESA CCI SM product.•MPDI and VOD performed better than RZSM to monitor LFMC in the Mediterranean region.•VARI, SAVI and VOD outperformed MPDI to capture the LFMC dynamics
Chemical etching with fluoride ions is a new approach for secondary porosity engineering of aluminosilicate zeolite frameworks. We show that diluted HF solutions extract preferentially aluminum from ...zeolite frameworks. The Brønsted acidity of ZSM-5 treated in such a way decreases, while its structure is unaffected after an HF treatment. With higher HF concentrations, the number of undissociated HF molecules and the concentration of HF2 – ions, extracting indiscriminately Al and Si, increase. The addition of NH4F shifts the chemical equilibria to produce more HF2 –, avoiding the use of highly concentrated HF solutions; it also suppresses HF dissociation. The etching selectivity of such solutions is concentration-independent and extracts indiscriminately both framework Si and Al. Zeolite dissolution in NH4F-HF solutions starts preferentially at small intergrowth domains and goes deeply in the crystals without a substantial increase of the external surface area. Macropores are produced without altering zeolite acidity. Hierarchical materials obtained by these two approaches are characterized extensively by complementary methods and the catalytic impact illustrated in the m-xylene conversion.
In this millennium, global drylands face a myriad of problems that present tough research, management, and policy challenges. Recent advances in dryland development, however, together with the ...integrative approaches of global change and sustainability science, suggest that concerns about land degradation, poverty, safeguarding biodiversity, and protecting the culture of 2.5 billion people can be confronted with renewed optimism. We review recent lessons about the functioning of dryland ecosystems and the livelihood systems of their human residents and introduce a new synthetic framework, the Drylands Development Paradigm (DDP). The DDP, supported by a growing and well-documented set of tools for policy and management action, helps navigate the inherent complexity of desertification and dryland development, identifying and synthesizing those factors important to research, management, and policy communities.
Summary Background We evaluated whether performing contrast-enhanced breast MRI in addition to mammography and/or ultrasound in patients with nonpalpable suspicious breast lesions improves breast ...cancer management. Methods The MONET – study (MR mammography of nonpalpable breast tumours) is a randomised controlled trial in patients with a nonpalpable BIRADS 3–5 lesion. Patients were randomly assigned to receive routine medical care, including mammography, ultrasound and lesion sampling by large core needle biopsy or additional MRI preceding biopsy. Patients with cancer were referred for surgery. Primary end-point was the rate of additional surgical procedures (re-excisions and conversion to mastectomy) in patients with a nonpalpable breast cancer. Findings Four hundred and eighteen patients were randomised, 207 patients were allocated to MRI, and 211 patients to the control group. In the MRI group 74 patients had 83 malignant lesions, compared to 75 patients with 80 malignant lesions in the control group. The primary breast conserving surgery (BCS) rate was similar in both groups; 68% in the MRI group versus 66% in the control group. The number of re-excisions performed because of positive resection margins after primary BCS was increased in the MRI group; 18/53 (34%) patients in the MRI group versus 6/50 (12%) in the control group ( p = 0.008). The number of conversions to mastectomy did not differ significantly between groups. Overall, the rate of an additional surgical intervention (BCS and mastectomy combined) after initial breast conserving surgery was 24/53 (45%) in the MRI group versus 14/50 (28%) in the control group ( p = 0.069). Interpretation Addition of MRI to routine clinical care in patients with nonpalpable breast cancer was paradoxically associated with an increased re-excision rate. Breast MRI should not be used routinely for preoperative work-up of patients with nonpalpable breast cancer.
A Neural Network (NN) algorithm was developed to estimate global surface soil moisture for April 2015 to March 2017 with a 2–3day repeat frequency using passive microwave observations from the Soil ...Moisture Active Passive (SMAP) satellite, surface soil temperatures from the NASA Goddard Earth Observing System Model version 5 (GEOS-5) land modeling system, and Moderate Resolution Imaging Spectroradiometer-based vegetation water content. The NN was trained on GEOS-5 soil moisture target data, making the NN estimates consistent with the GEOS-5 climatology, such that they may ultimately be assimilated into this model without further bias correction. Evaluated against in situ soil moisture measurements, the average unbiased root mean square error (ubRMSE), correlation and anomaly correlation of the NN retrievals were 0.037m3m−3, 0.70 and 0.66, respectively, against SMAP core validation site measurements and 0.026m3m−3, 0.58 and 0.48, respectively, against International Soil Moisture Network (ISMN) measurements. At the core validation sites, the NN retrievals have a significantly higher skill than the GEOS-5 model estimates and a slightly lower correlation skill than the SMAP Level-2 Passive (L2P) product. The feasibility of the NN method was reflected by a lower ubRMSE compared to the L2P retrievals as well as a higher skill when ancillary parameters in physically-based retrievals were uncertain. Against ISMN measurements, the skill of the two retrieval products was more comparable. A triple collocation analysis against Advanced Microwave Scanning Radiometer 2 (AMSR2) and Advanced Scatterometer (ASCAT) soil moisture retrievals showed that the NN and L2P retrieval errors have a similar spatial distribution, but the NN retrieval errors are generally lower in densely vegetated regions and transition zones.
•Development of SMAP neural network soil moisture retrieval product•Evaluating retrievals and model soil moisture versus core and sparse in situ sites•Comparable skill of SMAP neural network and official SMAP retrieval products•Comparison of global error patterns from triple collocation analysis
Very intense neutrino beams and large neutrino detectors will be needed in order to enable the discovery of CP violation in the leptonic sector. We propose to use the proton linac of the European ...Spallation Source currently under construction in Lund, Sweden, to deliver, in parallel with the spallation neutron production, a very intense, cost effective and high performance neutrino beam. The baseline program for the European Spallation Source linac is that it will be fully operational at 5 MW average power by 2022, producing 2 GeV 2.86 ms long proton pulses at a rate of 14 Hz. Our proposal is to upgrade the linac to 10 MW average power and 28 Hz, producing 14 pulses/s for neutron production and 14 pulses/s for neutrino production. Furthermore, because of the high current required in the pulsed neutrino horn, the length of the pulses used for neutrino production needs to be compressed to a few μs with the aid of an accumulator ring. A long baseline experiment using this Super Beam and a megaton underground Water Cherenkov detector located in existing mines 300–600 km from Lund will make it possible to discover leptonic CP violation at 5 σ significance level in up to 50% of the leptonic Dirac CP-violating phase range. This experiment could also determine the neutrino mass hierarchy at a significance level of more than 3 σ if this issue will not already have been settled by other experiments by then. The mass hierarchy performance could be increased by combining the neutrino beam results with those obtained from atmospheric neutrinos detected by the same large volume detector. This detector will also be used to measure the proton lifetime, detect cosmological neutrinos and neutrinos from supernova explosions. Results on the sensitivity to leptonic CP violation and the neutrino mass hierarchy are presented.
A search for the pair production of first-generation scalar leptoquarks is performed using proton-proton collision data recorded at 13 TeV center-of-mass energy with the CMS detector at the LHC. The ...data correspond to an integrated luminosity of 35.9 fb−1. The leptoquarks are assumed to decay promptly to a quark and either an electron or a neutrino, with branching fractions β and 1−β, respectively. The search targets the decay final states comprising two electrons, or one electron and large missing transverse momentum, along with two quarks that are detected as hadronic jets. First-generation scalar leptoquarks with masses below 1435 (1270) GeV are excluded for β=1.0(0.5). These are the most stringent limits on the mass of first-generation scalar leptoquarks to date. The data are also interpreted to set exclusion limits in the context of an R-parity violating supersymmetric model, predicting promptly decaying top squarks with a similar dielectron final state.
The 8B+64Zn reaction at 38.5 MeV has been studied at HIE-ISOLDE CERN to investigate proton halo effect on the reaction dynamics. For the first time it was used the only existing post-accelerated 8B ...beam. The measured elastic scattering angular distribution showed a small suppression of the Coulomb-nuclear interference peak, opposite to what observed for the one-neutron halo nucleus 11Be on the same target where a large suppression was observed instead. Inclusive angular and energy distributions of breakup fragments were also measured showing that, both, elastic and non-elastic breakup contribute. The presence of the additional Coulomb interactions halo-core and halo-target in 8B makes the reaction dynamics in this proton-halo nucleus different than the neutron-halo case.
Background and Objective: Focused Ion Beam - Scanning Electron Microscopy (FIB-SEM) allows three-dimensional ultrastructural analysis of cells and tissues at the nanoscale. The technique iteratively ...removes a section of the sample with a FIB and takes an SEM image from the exposed surface. The section thickness is usually higher than the image pixel size to reduce acquisition time, thus resulting in anisotropic resolution. In this work, we explore novel interpolation methods along the sectioning direction to produce isotropic resolution and facilitate proper interpretation of the FIB-SEM 3D volumes.
Methods: Classical interpolation methods are usually applied in this context under the assumption that the changes through successive images are relatively smooth. However, the actual 3D arrangement of the structures in the sample may cause significant changes in the biological features between consecutive images of the FIB-SEM stacks. We have developed a novel interpolation strategy that accounts for this variation by using the Optical Flow (OF) to estimate it. As an intermediate stage, OF-compensated images are produced by aligning the spatial regions of the biological structures. Interpolated images are then generated from these OF-compensated images. The final isotropic stack is assembled by interleaving the interpolated images with the original images of the anisotropic stack.
Results: OF-driven and classical interpolation methods were compared using an objective assessment based on Pearson Correlation Coefficient (PCC) and a qualitative evaluation based on visual results, using public datasets and representative anisotropy conditions. The objective assessment demonstrated that the OF-driven interpolation always yields higher PCC values, with interpolated images closer to the ground truth. The qualitative evaluation corroborated those results and confirmed that classical interpolation may blur areas with substantial changes between consecutive images whereas OF-driven interpolation provides sharpness.
Conclusions: We have developed an OF-driven interpolation approach to generating FIB-SEM stacks with isotropic resolution from experimental anisotropic data. It adapts to the rapid variation of the biological structures observed through the images of the FIB-SEM stack. Our approach outperforms classical interpolation and manages to produce sharp interpolated views in cases where there are significant changes between consecutive experimental images.
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