The dynamic interplay between intramammary IgG, formation of antigen-IgG complexes and effector immune cell function is essential for immune homeostasis within the bovine mammary gland. We explore ...how changes in the recognition and binding of anti-LPS IgG to the glycolipid “functional” core in milk from healthy or clinically diagnosed Escherichia coli (E. coli) mastitis cows’ controls endotoxin function. In colostrum, we found a varied anti-LPS IgG repertoire and novel soluble LPS/IgG complexes with direct IgG binding to the LPS glycolipid core. These soluble complexes, absent in milk from healthy lactating cows, were evident in cows diagnosed with E. coli mastitis and correlated with endotoxin-driven inflammation. E. coli mastitis milk displayed a proportional reduction in anti-LPS glycolipid core IgG compared to colostrum. Milk IgG extracts showed that only colostrum IgG attenuated LPS induced endotoxin activity. Furthermore, LPS-stimulated reactive oxygen species (ROS) in milk granulocytes was only suppressed by colostrum IgG, while IgG extracts of neither colostrum nor E. coli mastitis milk influenced N-formylmethionine-leucyl-phenylalanine (fMLP)-stimulated ROS in LPS primed granulocytes. Our findings support bovine intramammary IgG diversity in health and in response to E. coli infection generate milk anti-LPS IgG repertoires that coordinate appropriate LPS innate-adaptive immune responses essential for animal health.
The CMS experiment at the LHC features a two-level trigger system. Events accepted by the first level trigger, at a maximum rate of 100 kHz, are read out by the Data Acquisition system (DAQ), and ...subsequently assembled in memory in a farm of computers running a software high-level trigger (HLT), which selects interesting events for offline storage and analysis at a rate of order few hundred Hz. The HLT algorithms consist of sequences of offline-style reconstruction and filtering modules, executed on a farm of 0(10000) CPU cores built from commodity hardware. Experience from the operation of the HLT system in the collider run 2010/2011 is reported. The current architecture of the CMS HLT, its integration with the CMS reconstruction framework and the CMS DAQ, are discussed in the light of future development. The possible short- and medium-term evolution of the HLT software infrastructure to support extensions of the HLT computing power, and to address remaining performance and maintenance issues, are discussed.
The error and alarm system for the data acquisition of the Compact Muon Solenoid (CMS) at CERN was successfully used for the physics runs at Large Hadron Collider (LHC) during first three years of ...activities. Error and alarm processing entails the notification, collection, storing and visualization of all exceptional conditions occurring in the highly distributed CMS online system using a uniform scheme. Alerts and reports are shown on-line by web application facilities that map them to graphical models of the system as defined by the user. A persistency service keeps a history of all exceptions occurred, allowing subsequent retrieval of user defined time windows of events for later playback or analysis. This paper describes the architecture and the technologies used and deals with operational aspects during the first years of LHC operation. In particular we focus on performance, stability, and integration with the CMS sub-detectors.
The data-acquisition (DAQ) system of the CMS experiment at the LHC performs the read-out and assembly of events accepted by the first level hardware trigger. Assembled events are made available to ...the high-level trigger (HLT), which selects interesting events for offline storage and analysis. The system is designed to handle a maximum input rate of 100 kHz and an aggregated throughput of 100 GB/s originating from approximately 500 sources and 10^8 electronic channels. An overview of the architecture and design of the hardware and software of the DAQ system is given. We report on the performance and operational experience of the DAQ and its Run Control System in the first two years of collider runs of the LHC, both in proton-proton and Pb-Pb collisions. We present an analysis of the current performance, its limitations, and the most common failure modes and discuss the ongoing evolution of the HLT capability needed to match the luminosity ramp-up of the LHC.
The CMS experiment at the LHC features over 2'500 devices that need constant monitoring in order to ensure proper data taking. The monitoring solution has been migrated from Nagios to Icinga, with ...several useful plugins. The motivations behind the migration and the selection of the plugins are discussed.
Upgrade of the CMS Event Builder Bauer, G; Behrens, U; Bowen, M ...
Journal of physics. Conference series,
01/2012, Letnik:
396, Številka:
1
Journal Article
Recenzirano
Odprti dostop
The Data Acquisition system of the Compact Muon Solenoid experiment at CERN assembles events at a rate of 100 kHz, transporting event data at an aggregate throughput of 100 GB/s. By the time the LHC ...restarts after the 2013/14 shut-down, the current computing and networking infrastructure will have reached the end of their lifetime. This paper presents design studies for an upgrade of the CMS event builder based on advanced networking technologies such as 10/40 Gb/s Ethernet and Infiniband. The results of performance measurements with small-scale test setups are shown.
Monte Carlo production in CMS has received a major boost in performance and scale since the past CHEP06 conference. The production system has been re-engineered in order to incorporate the experience ...gained in running the previous system and to integrate production with the new CMS event data model, data management system and data processing framework. The system is interfaced to the two major computing Grids used by CMS, the LHC Computing Grid (LCG) and the Open Science Grid (OSG). Operational experience and integration aspects of the new CMS Monte Carlo production system is presented together with an analysis of production statistics. The new system automatically handles job submission, resource monitoring, job queuing, job distribution according to the available resources, data merging, registration of data into the data bookkeeping, data location, data transfer and placement systems. Compared to the previous production system automation, reliability and performance have been considerably improved. A more efficient use of computing resources and a better handling of the inherent Grid unreliability have resulted in an increase of production scale by about an order of magnitude, capable of running in parallel at the order of ten thousand jobs and yielding more than two million events per day.
Monte Carlo production for the CMS experiment is carried out in a distributed computing environment; the goal of producing 30M simulated events per month in the first half of 2007 has been reached. A ...brief overview of the production operations and statistics is presented.
The Physics Analysis eXpert (PAX) is an open source toolkit for high energy
physics analysis. The C++ class collection provided by PAX is deployed in a
number of analyses with complex event ...topologies at Tevatron and LHC. In this
article, we summarize basic concepts and class structure of the PAX kernel. We
report about the most recent developments of the kernel and introduce two new
PAX accessories. The PaxFactory, that provides a class collection to facilitate
event hypothesis evolution, and VisualPax, a Graphical User Interface for PAX
objects.
The Physics Analysis eXpert (PAX) is an open source toolkit for high energy physics analysis. The C++ class collection provided by PAX is deployed in a number of analyses with complex event ...topologies at Tevatron and LHC. In this article, we summarize basic concepts and class structure of the PAX kernel. We report about the most recent developments of the kernel and introduce two new PAX accessories. The PaxFactory, that provides a class collection to facilitate event hypothesis evolution, and VisualPax, a Graphical User Interface for PAX objects.
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