The present study was designated to evaluate the in vitro antidermatophyte activity of extracts from leaves of
Piper regnellii as well as of the bioactivity-directed isolation of neolignans. The ...antifungal assay was performed by microdilution techniques. The hydroalcoholic extract of
Piper regnellii leaves presented a strong activity against the dermatophyte fungi
Trichophyton mentagrophytes, Trichophyton rubrum,
Microsporum canis and
Microsporum gypseum with MICs of 15.62, 15.62, 15.62 and 62.5
μg/ml, respectively. On light microscopy and scanning electron microscopy of nail fragments not exposed to hydroalcoholic extract of
Piper regnelli leaves, well-formed and extensive mycelial growth was seen. On nail fragments exposed to hydroalcoholic extract at concentrations more than 1.2
mg/ml and then inoculated with spore suspension, growth was not seen. The hydroalcoholic extract was fractionated on silica gel in to nine fractions. The active chloroform fraction was lyophilized and chromatographed by column chromatography on silica gel. Structures were established by comparison with literature data and identified as eupomatenoid-3 and eupomatenoid-5. The pure compounds showed strong activity on
Trichophyton rubrum with MIC of 50 and 6.2
μg/ml, respectively. Comparing the activity of the active chloroform fraction obtained from hydroalcoholic crude extract with that of isolated compound eupomatenoid-5, it is clear that this showed the same results against
Trichophyton rubrum. The results showed that the plant could be explored for possible antifungal agents and provides preliminary scientific validation for the traditional medicinal use of this plant.
The increased amount of software in embedded systems allied to constraints as tight time-to-market and lower costs have motivated the investigation of strategies to manage embedded software design ...and its complexity. UML and Simulink are considered attractive for the embedded domain, a fact that motivates researchers to aim at finding a way to exploit the benefits of both languages. This paper proposes an automatic translation from UML diagrams to Simulink CAAM models, allowing designers to use UML as modeling language and to use facilities for simulation and code generation based on Simulink.
