•This ESMO Guideline provides key recommendations on the role of PROMs during the care of patients with cancer.•It covers the use of PROMs in patients with cancer from the start of active treatment ...during follow-up and at the end of life.•Recommendations are based on available scientific evidence and the authors’ collective expert consensus.•Authorship includes a multidisciplinary group of experts from Europe, North America, Asia and Australia.
The hematopoietic niche is a supportive microenvironment composed of distinct cell types, including specialized vascular endothelial cells that directly interact with hematopoietic stem and ...progenitor cells (HSPCs). The molecular factors that specify niche endothelial cells and orchestrate HSPC homeostasis remain largely unknown. Using multi-dimensional gene expression and chromatin accessibility analyses in zebrafish, we define a conserved gene expression signature and cis-regulatory landscape that are unique to sinusoidal endothelial cells in the HSPC niche. Using enhancer mutagenesis and transcription factor overexpression, we elucidate a transcriptional code that involves members of the Ets, Sox, and nuclear hormone receptor families and is sufficient to induce ectopic niche endothelial cells that associate with mesenchymal stromal cells and support the recruitment, maintenance, and division of HSPCs in vivo. These studies set forth an approach for generating synthetic HSPC niches, in vitro or in vivo, and for effective therapies to modulate the endogenous niche.
Display omitted
•Multi-dimensional expression analysis identifies endothelial signatures for HSPC niches•Study defines cis-regulatory landscape underlying niche endothelial identity•3-factor combination induces ectopic niche endothelial cells that support HSPCs
Hagedorn et al. use a combination of genomic techniques to elucidate an endothelial signature unique to blood stem cell niches. They define the underlying cis-regulatory landscape and a transcription factor combination that can reprogram embryonic cells into niche endothelial cells that can recruit and support blood stem cells.
•The world's deepest electrical resistance tomography (ERT) array.•A challenging environment of high temperature, high pressure and low pH.•Effective data processing methods to salvage extremely ...noisy data.•Effective detection of CO2 breakthroughs and saturation changes with time.•A near real time remote monitoring tool for tracking CO2 migration with time lapse tomographic images of CO2 saturation.
Deep geologic sequestration of carbon dioxide (CO2) is being evaluated internationally to mitigate the impact of greenhouse gases produced during oil- and coal-based energy generation and manufacturing. Natural gas producing fields are particularly attractive sites for sequestration activities owing to the assumption that the same geologic barrier or cap rock permitting the subsurface regime to act as a long term natural gas reservoir will also serve to permanently contain the injected supercritical CO2. Electrical resistance tomography (ERT) can potentially track the movement and concentration of the injectate as well as the degree of geologic containment using time lapse electrical resistivity changes resulting from injecting the super-critical fluid into the reservoir formation. An experimental cross-well ERT system operated successfully for more than one year obtaining time lapse electrical resistivity images during the injection of approximately one-million tons of CO2 at a depth exceeding 3000m in an oil and gas field in Cranfield, MS, representing the deepest application of the method to date. When converted to CO2 saturation, the resultant images provide information about the movement of the injected CO2 within a complex geologic formation and the development of the saturation distribution with time. ERT demonstrated significant potential for near real-time assessment of the degree of geologic containment and for updating risk analyses of the sequestration process. Furthermore, electrical resistivity imaging of the developing CO2 distribution may provide crucial input about the developing reservoir pressure field that is required for active reservoir management to prevent the occurrence of cap-rock-damaging seismic activity.
PDF
