Young and senescent rats were exposed to audiogenic stress for 6 h running and the effects of the treatment on the atrial myocardium were evaluated. In both young and senescent animals subcellular ...alterations of atrial cardiomyocytes were seen, consisting mainly of changes at the mitochondrial level. In particular, sections from young treated rats exhibited swollen mitochondria with lysis of their cristae and dissolution of the matrix. An enlargement was observed of the space between the outer and inner membranes of the mitochondria, which also showed an increase in size. In addition, aged treated specimens revealed decreased density of the sarcoplasm and marked heterogeneity in the myofibril pattern with ruptures and loss of continuity of the myofilaments.
A series of 3-substituted 1,2,4triazino4,3-cbenzimidazoles V were prepared and tested at the central benzodiazepine receptor (BzR). These compounds were designed as rigid analogues of the previously ...described N-benzylindolylglyoxylylamide derivatives IV. The title compounds V showed an affinity which depended directly on the presence of the N(10)-H group and an aromatic ring at position 3. Some of them elicited a 2- or 3-fold higher affinity with respect to that of the indolylglyoxylylamide derivatives IV (R = H). The GABA ratio and 35S-tert-butylcyclophosphorothionate binding data revealed an efficacy profile of partial inverse agonists/antagonists for compounds 1c,e,f,j,k, and of a partial agonist for 2c. This last compound proved to be effective in antagonizing pentylenetetrazole-induced seizures in mice. Attempts were made to interpret the structure−affinity relationships of compounds V in the light of possible tautomeric equilibria involving the ligands.
The present study compares, from a morphological point of view, the possible differences between perfusion- and immersion-fixation techniques performed on rat myocardium in order to evaluate their ...reliability. The data we obtained showed that when the two techniques are carried out with particular care, they do not present significant differences in tissue preservation. Furthermore, we also evaluated the ultrastructural of rat myocardium at the end of in vitro experiments. In this case, signs of subcellular changes, such as vacuolar degeneration, swollen mitochondria, intermyofibrillar myelin-like figures and enlargements of non specialized junctions of the intercalated discs can frequently occur. So, our results indicate that the morphological integrity of myocardium is not completely preserved during in vitro investigations.
Male rats were exposed to noise for 6 running hours and the effects of pretreatment with the benzodiazepine diazepam on the adrenal gland were evaluated. Ultrastructural examination showed that, ...after noise exposure, zona reticularis cells resulted the more affected, exhibiting areas of diluted cytoplasm, disarranged endoplasmic reticulum, membrane vestigia and some altered mitochondria; diluted cytoplasmic areas appeared in noradrenaline-storing cells, too. On the contrary, zona reticularis cells from diazepam-pretreated and noise-exposed rats resulted significantly less altered, as well as the noradrenaline-storing cells. The present findings indicate that diazepam is able to exert some protective action on adrenal gland alterations due to noise exposure.
In the present research the effect of repeated microwave exposure on the noradrenergic pattern by histofluorescence method and on receptor-mediated responses using alpha and beta agonists in ...myocardium and aorta of young-adult and aged rats was studied. Young-adult irradiated rats showed an increase in noradrenergic innervation more marked in myocardial tissue, while an increase in maximal response to the agonist was found only at aortic level. Aged stressed rats exhibited an increase in fluorescent fibres at atrial and aortic level, but in the atrial section this increase was found to be less evident than in young-adult animals. Functional data in aged rats revealed a more marked decrease in maximal response ratio (M.R.R.) of myocardial tissue than in young-adult rats, together with a noticeable decrease in maximal response at aortic level. These results indicate no direct correlation between morphological and functional data. Participation of both central and peripheral mechanisms is suggested.
New fluorescent ligands for adenosine receptors are described; these compounds were obtained by the insertion, in the N
6 position of NECA (a potent adenosine agonist), of dansylaminoalkyl moieties ...with alkyl spacers of increasing carbon chain length (from 3 to 12). Among them, the compound with a C6 alkyl spacer proved to be the most interesting one, showing a marked selectivity for the A
1 receptor subtype; furthermore, in fluorescence microscopy assays it proved to be able to visualize and localize this receptor subtype at the level of the molecular layer of the rat cerebellar cortex.
New fluorescent ligands for adenosine receptors are described. Among them, compound 1c proved to be the most interesting one, as it showed a marked selectivity for the A
1 receptor subtype and in fluorescence microscopy assays proved to be able to visualize and localize this receptor subtype at the level of the molecular layer of the rat cerebellar cortex.
Un groupe de médecins généralistes et multidisciplinaire de spécialistes contribue à définir les différentes formes de sinusites ou rhinosinusites aiguës de l'enfant à partir des principaux symptômes ...et signes cliniques. Le rôle des pathologies associées telles que l'allergie, les troubles immunitaires est envisagé. L'incidence, la présentation clinique et la prise en charge des complications sont présentées. Les indications des examens radiologiques et biologiques sont analysées. La prise en charge médicale en particulier, la place et le type des antibiotiques sont discutés. Des propositions de prise en charge selon des situations cliniques rencontrées en pratique sont présentées.
A multidisciplinary panel of specialists and general practitioners provided definitions for the different forms of acute pediatric rhinosinusitis, with a description of the main symptoms and signs. They emphasized the role of concomitant systemic diseases, such as allergy and immunological disorders. Incidence, description, and management of complications are presented. They also provided the indications for radiological examination and microbiological investigations. The adequate medical management, particularly the place and the type of antibiotics, is analyzed and discussed, and guidelines for practical situations are suggested.
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