•Panama Canal Watershed is one of the most important hydrological systems worldwide.•HJ-Biplot approach proves to be an efficient tool for water quality studies.•HJ-Biplot approach allowed a robust ...classification of sampling points.•HJ-Biplot has very few restrictions and good performance for matrices inspection.•The multivariate HJ-Biplot allows relating physicochemical and biological variables.
Water quality is a sensitive topic of worldwide concern that is defined by a series of physical, chemical and biological characteristics. The complex nature for studying water quality requires finding simple models to identify the variables that influence it the most. Therefore, the use of multivariate analysis techniques will be of immense help to find relationships and conclusions which aid us to determine the state of water quality through biological, physical and chemical indicators. In this investigation, the following variables were evaluated: Temperature, pH, transparency, turbidity, nitrates, orthophosphates, phosphorus, total nitrogen, chlorophyll a, solar radiation, dissolved oxygen and microcystins. These determined water quality at the sample sites, Gamboa and Paraiso. The results obtained through the application of a two-way multivariate analysis method called HJ-Biplot reflect variableś relationships of chemical, physical and biological compositions. Furthermore, results conform two clusters of sample points that satisfactorily match to the region seasons. Cluster 1 is characterized by the presence of the following variables: pH, transparency, chlorophyll a, oxygen and temperature. On the other hand, cluster 2 comprises the following variables: nitrate, orthophosphates, turbidity and P-total. They are all parameters that suffer variations in the rainy season, and that, in turn, can influence the presence of ciabobacteria with toxigenic potential. Furthermore, this study demonstrates that the multivariate statistical methods are valuable for interpreting complex data sets, specifically, for water quality monitoring network.
Parkinson's disease (PD) is a neurodegenerative disorder for which available treatments provide symptom relief but do not stop disease progression. Mitochondria, and in particular mitochondrial ...dynamics, have been postulated as plausible pharmacological targets. Mitochondria-targeted antioxidants have been developed to prevent mitochondrial oxidative damage, and to alter the involvement of reactive oxygen species (ROS) in signaling pathways. In this study, we have dissected the effect of MitoQ, which is produced by covalent attachment of ubiquinone to a triphenylphosphonium lipophilic cation by a ten carbon alkyl chain. MitoQ was tested in an in vitro PD model which involves addition of 6-hydroxydopamine (6-OHDA) to SH-SY5Y cell cultures. At sublethal concentrations of 50μM, 6-OHDA did not induce increases in protein carbonyl, mitochondrial lipid peroxidation or mitochondrial DNA damage. However, after 3h of treatment, 6-OHDA disrupts the mitochondrial morphology and activates the machinery of mitochondrial fission, but not fusion. Addition of 6-OHDA did not increase the levels of fission 1, mitofusins 1 and 2 or optic atrophy 1 proteins, but does lead to the translocation of dynamin related protein 1 from the cytosol to the mitochondria. Pre-treatment with MitoQ (50nM, 30min) results in the inhibition of the mitochondrial translocation of Drp1. Furthermore, MitoQ also inhibited the translocation of the pro-apoptotic protein Bax to the mitochondria. These findings provide mechanistic evidence for a role for redox events contributing to mitochondrial fission and suggest the potential of mitochondria-targeted therapeutics in diseases that involve mitochondrial fragmentation due to oxidative stress.
Concentrations of heavy metals (Cu, Zn, Cd, Pb and As) were measured in water, sediment and two fish species,
Sparus aurata and
Solea senegalensis, from the estuary of Tinto and Odiel rivers in ...Huelva (Spain), one of the most metallic polluted estuaries in Europe.
As a forward step to understand metal bioavailability and assess the potential impact on aquatic biota, a study of heavy metal speciation of sediments and water were achieved. High levels of total and dissolved Zn and Cu were found in water and high pollution of Zn, Pb, As and Cu were found in sediments. Availability of metals was established as following ranking: Cd
>
Zn
>
Cu
>
Pb in both water and sediment. In addition, the effect of this pollution was studied by evaluation of metal bioaccumulations and correlations obtained between metal levels in fractions of water and sediment and metal levels in fish tissues. High Cu and Zn levels were observed in liver tissue of both species, in according with higher total content and more available metals in water and sediment. Correlations among metal content in tissues and different fractions of metal in water for
S. aurata and sediment for
S. senegalensis were found.
Drug use implies important challenges related to HIV management, particularly due to an increased risk of potential interactions between antiretroviral therapy (ART) and illicit drugs (pDDIs). This ...study analyses the prevalence and severity of pDDIs among people living with HIV (PLHIV). It also explores their awareness of pDDIs and their beliefs about the toxicity that they may cause, as well as the impact of pDDIs on selected health variables. We conducted an on-line cross-sectional survey across 33 Spanish hospitals and NGOs to collect demographics and clinical data. pDDIs were checked against the Interaction Checker developed by Liverpool University. The sample of the present study was composed of 694 PLHIV who used illicit drugs. They represented 49.5% of the 1,401 PLHIV that participated in the survey. After excluding 38 participants due to lack of information on their ART or illicit drug use, 335 (51.1%) participants consuming drugs presented with some potentially significant pDDIs between their ART and illicit drugs, with a mean of 2.1±1.7 (1-10) pDDIs per patient. The drugs most frequently involved in pDDIs were cocaine, cannabis, MDMA and nitrates ("poppers"). The prevalence of pDDIs across ART regimens was: protease inhibitors (41.7%); integrase inhibitor-boosted regimens (32.1%), and non-nucleoside reverse transcriptase inhibitors (26.3%). An awareness of pDDIs and beliefs about their potential toxicity correlated positively with intentional non-adherence (p<0.0001). Participants with pDDIs exhibited a higher prevalence of intentional non-adherence (2.19±1.04 vs. 1.93±0.94; p = 0.001). The presence of pDDIs was not associated with poorer results in the clinical variables analysed. A significant proportion of PLHIV who use drugs experience pDDIs, thereby requiring close monitoring. pDDIs should be considered in the clinical management of HIV patients. Adequate information about pDDIs and indicators about how to manage ART when PLHIV use drugs could improve ART non-adherence.
This study assessed the prevalence and patterns of drug use among people living with HIV (PLHIV) in Spain. We conducted an observational cross-sectional study including 1401 PLHIV. Data were ...collected through 33 sites across Spain using an online computer-assisted self-administered interview. The survey measured use of illicit drugs and other substances, treatment adherence and health-related variables. To analyse patterns of drug use we performed cluster analysis in two stages. The most frequently consumed substances were: alcohol (86.7%), tobacco (55.0%), illicit drugs (49.5%), other substances (27.1%). The most prevalent illicit drugs used were cannabis (73.8%), cocaine powder (53.9%), and poppers (45.4%). Results found four clusters of PLHIV who used drugs. Two of them were composed mainly of heterosexuals (HTX): Cluster 1 (n = 172) presented the lowest polydrug use and they were mainly users of cannabis, and Cluster 2 (n = 84) grouped mostly men who used mainly heroin and cocaine; which had the highest percentage of people who inject drugs and presented the lowest level of treatment adherence (79.8±14.2; p < .0001). The other two clusters were composed mainly of men who have sex with men (MSM), who were mostly users of recreational drugs. Cluster 3 (n = 285) reported moderate consumption, both regarding frequency and diversity of drugs used, while Cluster 4 (n = 153) was characterized by the highest drug polyconsumption (7.4±2.2; p < .0001), and 4 grouped MSM who injected recreational drugs, and who reported the highest frequency of use of drugs in a sexual context (2.6±0.8; p < .0001) and rates of sexually transmitted infections (1.8±1.1; p < .01). This is the largest multi-centre cross-sectional study assessing the current prevalence and patterns of drug use among PLHIV in Spain. The highest prevalence of drug use was found among MSM, although HTX who used heroin and cocaine (Cluster 2) had the most problems with adherence to HIV treatment and the worst health status.
The most likely routes for the thermal decomposition of biomass derived D-galacturonic acid leading to furfural were analyzed through comparison of the activation energy barriers associated with ...possible kinetic pathways. Dehydration steps were associated with significant activation barriers. The effect of a mesoporous catalyst with Brønsted acidity such as Al-SBA-15 was analyzed. The chemical structure of SBA-15 was optimized by means of a comparison from the experimental structural data previously reported in literature and the structural parameters obtained from DFT calculations at B3LYP/6–31 + G (d, p) level of several catalyst surface models. The bicyclic model structure obtained proved to be representative of framework species in SBA-15. The isomorphic substitution of Al in the SBA-15 model structure was made in order to generate Brønsted acidity. The acidity was studied for the dehydration of methanol, this reaction being representative of the dehydration processes that occur in biomass transformation to furfural. A higher Brønsted acidity strength was observed in free silanol type (Q
3
) and geminal silanol type (Q
2
) sites, formed by the isomorphic Al substitution of Si.
The impact of inflammatory rheumatic diseases on COVID-19 severity is poorly known. Here, we compare the outcomes of a cohort of patients with rheumatic diseases with a matched control cohort to ...identify potential risk factors for severe illness.
In this comparative cohort study, we identified hospital PCR+COVID-19 rheumatic patients with chronic inflammatory arthritis (IA) or connective tissue diseases (CTDs). Non-rheumatic controls were randomly sampled 1:1 and matched by age, sex and PCR date. The main outcome was severe COVID-19, defined as death, invasive ventilation, intensive care unit admission or serious complications. We assessed the association between the outcome and the potential prognostic variables, adjusted by COVID-19 treatment, using logistic regression.
The cohorts were composed of 456 rheumatic and non-rheumatic patients, in equal numbers. Mean age was 63 (IQR 53-78) years and male sex 41% in both cohorts. Rheumatic diseases were IA (60%) and CTD (40%). Most patients (74%) had been hospitalised, and the risk of severe COVID-19 was 31.6% in the rheumatic and 28.1% in the non-rheumatic cohort. Ageing, male sex and previous comorbidity (obesity, diabetes, hypertension, cardiovascular or lung disease) increased the risk in the rheumatic cohort by bivariate analysis. In logistic regression analysis, independent factors associated with severe COVID-19 were increased age (OR 4.83; 95% CI 2.78 to 8.36), male sex (1.93; CI 1.21 to 3.07) and having a CTD (OR 1.82; CI 1.00 to 3.30).
In hospitalised patients with chronic inflammatory rheumatic diseases, having a CTD but not IA nor previous immunosuppressive therapies was associated with severe COVID-19.
Global rankings help boost the international reputation of universities, which thus attempt to achieve good positions on them. These rankings attract great interest each year and are followed ...attentively by stakeholders in higher education. This paper investigates the trajectory of Spanish universities in the ARWU and THE rankings over the last 5 years using the dynamic biplot technique to study the relationship between a multivariate dataset obtained at more than one time point. The results demonstrate that Spanish universities achieve low positions on international rankings when analyzed using this multivariate and dynamic approach. Indeed, only a small percentage occupy good positions in both studied rankings and stand out in terms of some of the indicators, whereas most achieve weak scores in the global context. Spanish universities should attempt to improve this situation, since the prestige resulting from a good position on these lists will always be beneficial in terms of the visibility of both the universities themselves and the whole Spanish university system.
We describe and characterize an exceptionally large HIV-1 subtype B transmission cluster occurring in the Comunidad Valenciana (CV, Spain). A total of 1806 HIV-1 protease-reverse transcriptase ...(PR/RT) sequences from different patients were obtained in the CV between 2004 and 2014. After subtyping and generating a phylogenetic tree with additional HIV-1 subtype B sequences, a very large transmission cluster which included almost exclusively sequences from the CV was detected (n = 143 patients). This cluster was then validated and characterized with further maximum-likelihood phylogenetic analyses and Bayesian coalescent reconstructions. With these analyses, the CV cluster was delimited to 113 patients, predominately men who have sex with men (MSM). Although it was significantly located in the city of Valencia (n = 105), phylogenetic analyses suggested this cluster derives from a larger HIV lineage affecting other Spanish localities (n = 194). Coalescent analyses estimated its expansion in Valencia to have started between 1998 and 2004. From 2004 to 2009, members of this cluster represented only 1.46% of the HIV-1 subtype B samples studied in Valencia (n = 5/143), whereas from 2010 onwards its prevalence raised to 12.64% (n = 100/791). In conclusion, we have detected a very large transmission cluster in the CV where it has experienced a very fast growth in the recent years in the city of Valencia, thus contributing significantly to the HIV epidemic in this locality. Its transmission efficiency evidences shortcomings in HIV control measures in Spain and particularly in Valencia.
J. Neurochem. (2010) 114, 933-945. Despite the increasing knowledge of Alzheimer's disease (AD) management with novel pharmacologic agents, most of them are only transiently fixing symptomatic ...pathology. Currently there is rapid growth in the field of neuroprotective pharmacology and increasing focus on the involvement of mitochondria in this devastating disease. This review is directed at understanding the role of mitochondria-mediated pathways in AD and integrating basic biology of the mitochondria with knowledge of possible pharmacologic targets for AD treatment in an attempt to elucidate novel mitochondria-driven therapeutic interventions useful to both clinical and basic research.
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