Context. The study of the inner region of the Milky Way bulge is hampered by high interstellar extinction and extreme source crowding. Sensitive high angular resolution near-infrared imaging is ...needed to study stellar populations and their characteristics in such a dense and complex environment. Aims. We aim at investigating the stellar population in the innermost Galactic bulge, to study the star formation history in this region of the Galaxy. Methods. We used the 0.2″ angular resolution JHKs data from the GALACTICNUCLEUS survey to study the stellar population within two 8.0′×3.4′ fields, about 0.6° and 0.4° to the Galactic north of the Milky Way centre and to compare it with the one in the immediate surroundings of Sagittarius A*. We also characterise the absolute extinction and the extinction curve of the two fields. Results. The average interstellar extinction to the outer and the inner field is AKs ∼ 1.20 ± 0.08 mag and ∼1.48 ± 0.10 mag, respectively. We present Ks luminosity functions that are complete down to at least two magnitudes below the red clump (RC). We detect a feature in the luminosity functions that is fainter than the RC by 0.80 ± 0.03 and 0.79 ± 0.02 mag, respectively, in the Ks band. It runs parallel to the reddening vector. We identify the feature as the red giant branch bump. Fitting α-enhanced BaSTI luminosity functions to our data, we find that a single old stellar population of ∼12.8 ± 0.6 Gyr and Z = 0.040 ± 0.003 provides the best fit. Our findings thus show that the stellar population in the innermost bulge is old, similar to the one at larger distances from the Galactic plane, and that its metallicity is about twice solar at distances as short as about 60 pc from the centre of the Milky Way, similar to what is observed at about 500 pc from the Galactic Centre. Comparing the obtained metallicity with previous known values at larger latitudes (|b| > 2°), our results favour a flattening of the gradient at |b| < 2°. As a secondary result we obtain that the extinction index in the studied regions agrees within the uncertainties with our previous value of α = 2.30 ± 0.08 that was derived for the very Galactic centre.
Obesity severely affects human health, and the accompanying non-alcoholic fatty liver disease (NAFLD) is associated with high morbidity and mortality. Rapid and non-invasive methods to detect this ...condition may substantially improve clinical care.
We used liquid and gas chromatography-quadruple time-of-flight-mass spectrometry (LC/GC-QTOF-MS) analysis in a non-targeted metabolomics approach on the plasma from morbidly obese patients undergoing bariatric surgery to gain a comprehensive measure of metabolite levels. On the basis of these findings, we developed a method (GC-QTOF-MS) for the accurate quantification of plasma α-ketoglutarate to explore its potential as a novel biomarker for the detection of NAFLD.
Plasma biochemical differences were observed between patients with and without NAFLD indicating that the accumulation of lipids in hepatocytes decreased β-oxidation energy production, reduced liver function and altered glucose metabolism. The results obtained from the plasma analysis suggest pathophysiological insights that link lipid and glucose disturbances with α-ketoglutarate. Plasma α-ketoglutarate levels are significantly increased in obese patients compared with lean controls. Among obese patients, the measurement of this metabolite differentiates between those with or without NAFLD. Data from the liver were consistent with data from plasma. Clinical utility was assessed, and the results revealed that plasma α-ketoglutarate is a fair-to-good biomarker in patients (n=230). Other common laboratory liver tests used in routine application did not favourably compare.
Plasma α-ketoglutarate is superior to common liver function tests in obese patients as a surrogate biomarker of NAFLD. The measurement of this biomarker may potentiate the search for a therapeutic approach, may decrease the need for liver biopsy and may be useful in the assessment of disease progression.
The aim of the present work was to develop 3D discrete element models capable of simulating the observed flow of glass beads (simple glass spheres) and maize grains (represented as a combination of ...spheres) during their discharge from a small model silo. A preliminary model for each material was constructed based on values for variables measured in the laboratory or taken from the literature. The ability of the models to predict the flow of these materials was then tested by comparing their results with observed discharge flows. Three variables were recorded for this: the mean bulk density at the end of the filling phase, the discharge rate and the flow pattern. The comparison of the results for the last of these variables required the discharge process be filmed using a high speed camera in order to more easily recognise the details of the flow. The preliminary model for the glass beads made very reasonable predictions, but that for the maize grains required calibration. This involved modifying the values of the friction properties of the material until a model capable of making acceptable predictions was obtained. The results obtained highlighted the influence of friction properties on the characteristics of the discharge flow. Finally, some of the numerical results provided by the models were analysed in order to describe the flow characteristics and the behaviour of the discharge rate in more detail.
► Procedure for calibrating and validating 3D DEM models for silo discharge. ► Properties of materials (glass beads and maize grains) were experimentally measured. ► The influence of friction on bulk density, discharge rate and flow pattern was analysed. ► The flow pattern and the discharge rate were numerically analysed in detail.
Ensuring balanced distribution of chromosomes in gametes, meiotic recombination is essential for fertility in most sexually reproducing organisms. The repair of the programmed DNA double strand ...breaks that initiate meiotic recombination requires two DNA strand-exchange proteins, RAD51 and DMC1, to search for and invade an intact DNA molecule on the homologous chromosome. DMC1 is meiosis-specific, while RAD51 is essential for both mitotic and meiotic homologous recombination. DMC1 is the main catalytically active strand-exchange protein during meiosis, while this activity of RAD51 is downregulated. RAD51 is however an essential cofactor in meiosis, supporting the function of DMC1. This work presents a study of the mechanism(s) involved in this and our results point to DMC1 being, at least, a major actor in the meiotic suppression of the RAD51 strand-exchange activity in plants. Ectopic expression of DMC1 in somatic cells renders plants hypersensitive to DNA damage and specifically impairs RAD51-dependent homologous recombination. DNA damage-induced RAD51 focus formation in somatic cells is not however suppressed by ectopic expression of DMC1. Interestingly, DMC1 also forms damage-induced foci in these cells and we further show that the ability of DMC1 to prevent RAD51-mediated recombination is associated with local assembly of DMC1 at DNA breaks. In support of our hypothesis, expression of a dominant negative DMC1 protein in meiosis impairs RAD51-mediated DSB repair. We propose that DMC1 acts to prevent RAD51-mediated recombination in Arabidopsis and that this down-regulation requires local assembly of DMC1 nucleofilaments.
The repair of DNA double-strand breaks by recombination is key to the maintenance of genome integrity in all living organisms. Recombination can however generate mutations and chromosomal ...rearrangements, making the regulation and the choice of specific pathways of great importance. In addition to end-joining through non-homologous recombination pathways, DNA breaks are repaired by two homology-dependent pathways that can be distinguished by their dependence or not on strand invasion catalysed by the RAD51 recombinase. Working with the plant Arabidopsis thaliana, we present here an unexpected role in recombination for the Arabidopsis RAD51 paralogues XRCC2, RAD51B and RAD51D in the RAD51-independent single-strand annealing pathway. The roles of these proteins are seen in spontaneous and in DSB-induced recombination at a tandem direct repeat recombination tester locus, both of which are unaffected by the absence of RAD51. Individual roles of these proteins are suggested by the strikingly different severities of the phenotypes of the individual mutants, with the xrcc2 mutant being the most affected, and this is confirmed by epistasis analyses using multiple knockouts. Notwithstanding their clearly established importance for RAD51-dependent homologous recombination, XRCC2, RAD51B and RAD51D thus also participate in Single-Strand Annealing recombination.
An essential component of the homologous recombination machinery in eukaryotes, the RAD54 protein is a member of the SWI2/SNF2 family of helicases with dsDNA-dependent ATPase, DNA translocase, DNA ...supercoiling and chromatin remodelling activities. It is a motor protein that translocates along dsDNA and performs multiple functions in homologous recombination. In particular, RAD54 is an essential cofactor for regulating RAD51 activity. It stabilizes the RAD51 nucleofilament, remodels nucleosomes, and stimulates homology search and strand invasion activity of RAD51. Accordingly, deletion of RAD54 has dramatic consequences on DNA damage repair in mitotic cells. In contrast, its role in meiotic recombination is less clear. RAD54 is essential for meiotic recombination in Drosophila and C. elegans, but plays minor roles in yeast and mammals. We present here characterization of the roles of RAD54 in meiotic recombination in the model plant Arabidopsis thaliana. Absence of RAD54 has no detectable effect on meiotic recombination in otherwise wild-type plants but RAD54 becomes essential for meiotic DSB repair in absence of DMC1. In Arabidopsis, dmc1 mutants have an achiasmate meiosis, in which RAD51 repairs meiotic DSBs. Lack of RAD54 leads to meiotic chromosomal fragmentation in absence of DMC1. The action of RAD54 in meiotic RAD51 activity is thus mainly downstream of the role of RAD51 in supporting the activity of DMC1. Equivalent analyses show no effect on meiosis of combining dmc1 with the mutants of the RAD51-mediators RAD51B, RAD51D and XRCC2. RAD54 is thus required for repair of meiotic DSBs by RAD51 and the absence of meiotic phenotype in rad54 plants is a consequence of RAD51 playing a RAD54-independent supporting role to DMC1 in meiotic recombination.
Recombination establishes the chiasmata that physically link pairs of homologous chromosomes in meiosis, ensuring their balanced segregation at the first meiotic division and generating genetic ...variation. The visible manifestation of genetic crossing-overs, chiasmata are the result of an intricate and tightly regulated process involving induction of DNA double-strand breaks and their repair through invasion of a homologous template DNA duplex, catalysed by RAD51 and DMC1 in most eukaryotes. We describe here a RAD51-GFP fusion protein that retains the ability to assemble at DNA breaks but has lost its DNA break repair capacity. This protein fully complements the meiotic chromosomal fragmentation and sterility of Arabidopsis rad51, but not rad51 dmc1 mutants. Even though DMC1 is the only active meiotic strand transfer protein in the absence of RAD51 catalytic activity, no effect on genetic map distance was observed in complemented rad51 plants. The presence of inactive RAD51 nucleofilaments is thus able to fully support meiotic DSB repair and normal levels of crossing-over by DMC1. Our data demonstrate that RAD51 plays a supporting role for DMC1 in meiotic recombination in the flowering plant, Arabidopsis.
The application of numeric modelling for determining the impact of landfills needs for reliable emission source data. In this study, a methodology for the characterization of the emission profiles of ...the different sources present in landfills for emission factors determination, applying an indirect methodology, is presented. Ambient air concentrations of volatile organic compounds (VOCs), hydrogen sulphide (H2S) and ammonia (NH3) were determined in three potentially emission sources in Can Mata landfill (Hostalets de Pierola, Catalonia, Spain): dumping areas, pre-closed zone and leachate reservoir as well as in biogas, for the determination of emission factors. Multi-sorbent bed and Tenax TA tubes were used for a wide range of VOCs sampling, and analysis was conducted through TD-GC/MS. H2S and NH3 were sampled and analysed using Radiello passive samplers. The highest total VOC (TVOC) concentrations were found in dumping areas (0.7–3.5 mg m−3), followed by leachate reservoir (0.3–0.6 mg m−3) and pre-closed area (77–165 μg m−3). On the other hand, the highest H2S and NH3 concentrations were found in leachate reservoir, presenting values of 0.8–1.1 mg m−3 and 1.7–1.8 mg m−3, respectively. With the application of odour thresholds to the concentrations obtained, the most critical compounds regarding odour annoyances were determined. The highest odour units (O.U.) were found in leachate reservoir due to H2S concentrations, whereas VOCs contributed mainly to O.U. in the dumping areas. The obtained ambient air concentrations were used for the indirect determination of the emission factors through numerical modelling using a Eulerian dispersion model. The emission factors obtained for the landfill for TVOC, H2S and NH3 were in the range of 0.44–10.9 g s-1, 0.16–1.02 g s−1 and 0.23–1.82 g s−1, respectively, depending on the emission source. Reliable emission factors are crucial to obtain landfill impact maps, which are essential for the correct management of these facilities.
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•Landfill VOCs, H2S and NH3 emission factors were calculated using inverse methodology.•Highest TVOC concentrations (0.7–3.5 mg m−3) were found in the areas of dumping.•Alcohols, ketones, acids, aldehydes, aromatics & terpenes were the characteristic VOC.•Highest H2S concentrations (0.8–1.1 mg m−3) were found in leachate reservoir.•Emission factors: VOCs: 0.44–10.9 g s−1; H2S: 0.16–1.02 g s−1; NH3: 0.23–1.82 g s−1.
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