Abstract
The formation of long-lived reactive protein species of bovine serum albumin (BSA), ovalbumin, casein and casein hydrolyzate with a half-life of 3-5 hours was shown using chemiluminescence ...induced by X-ray radiation. It was found that long-lived reactive protein species are capable of generating reactive oxygen species (ROS) (H2O2, OH*, HO2*, 1O2) in the aquatic environment over a long period of time in vitro. The interaction of X-ray-irradiated BSA with DNA in vitro led to the formation of 8-oxoguanine (8-oxo-7,8-dihydroguanine), a biomarker of oxidative damage to DNA. Some natural antioxidants are effective scavengers of ROS (inosine, tryptophan, methionine and ascorbate). They protect DNA from the action of long-lived reactive protein species leading to ROS generation and the formation of 8-oxoguanine. The intravenous injection of X-ray radiation-induced, long-lived reactive protein species to rats, as well as the peroral and intraperitoneal administration of these products to mice, gave rise to cytogenetic injuries in the cells of their red bone marrow through the formation of micronuclei in polychromatophilic erythrocytes. The administration of the same natural antioxidants used for in vitro experiments soon after irradiation made it possible to effectively eliminate the genotoxic action of oxidative stress caused by radiation-induced, long-lived reactive protein species. Our data represent clear evidence that the oxidative damage to proteins induced by X-rays is directly involved in the induction of a response to DNA damage in rodents.
Activation of pentose phosphate pathway is an important factor of enhanced cell proliferation and tumor growth. Phosphoribosyl pyrophosphate synthetase (PRPS) is a key enzyme of this pathway and ...plays a central role in the synthesis of purines and pyrimidines. Hypoxia as well as ERN1 (from endoplasmic reticulum to nuclei-1) mediated endoplasmic reticulum stress response-signalling pathway is linked to the proliferation because the blockade of ERN1 suppresses tumor growth, including glioma. We studied the expression of different PRPS genes in glioma cells with ERN1 knockdown under hypoxic condition. It was shown that hypoxia decreases the expression of PRPS1 and PRPS2 genes in both types of glioma cells, being more pronounced in cells without ERN1 function, but PRPSAP1 and PRPSAP2 gene expressions are suppressed by hypoxia only in glioma cells with blockade of ERN1. Moreover, the blockade of endoribonuclease activity of ERN1 does not affect the expression of PRPS1 and PRPS2 as well as PPRS-associated protein genes in U87 glioma cells. At the same time, the induction of endoplasmic reticulum stress by tunicamycin in glioma cells with suppressed activity of ERN1 endoribonuclease decreases the expression level of PRPS1 and PRPS2 genes only. Results of this investigation clearly demonstrated that the expression of different genes encoding subunits of PRPS enzyme is affected by hypoxia in U87 glioma cells, but the effect of hypoxia is modified by suppression of endoplasmic reticulum stress signaling enzyme ERN1.
Abstract We present the first model-independent measurement of the CKM unitarity triangle angle ϕ 3 using B ± → D( K S 0 $$ {K}_{\mathrm{S}}^0 $$ π + π − π 0) K ± decays, where D indicates either a D ...0 or D ¯ $$ \overline{D} $$ 0 meson. Measurements of the strong-phase difference of the D → K S 0 $$ {K}_{\mathrm{S}}^0 $$ π + π − π 0 amplitude obtained from CLEO-c data are used as input. This analysis is based on the full Belle data set of 772 × 106 B B ¯ $$ \overline{B} $$ events collected at the Υ(4S) resonance. We obtain ϕ 3 = ( 5.7 − 8.8 + 10.2 $$ {5.7}_{-8.8}^{+10.2} $$ ±3.5±5.7) ° and the suppressed amplitude ratio r B = 0.323±0.147±0.023±0.051. Here the first uncertainty is statistical, the second is the experimental systematic, and the third is due to the precision of the strong-phase parameters measured from CLEO-c data. The 95% confidence interval on ϕ 3 is (−29.7, 109.5) ° , which is consistent with the current world average.
The experimental results on the ratios of branching fractions R(D)=B(Bover ¯→Dτ^{-}νover ¯_{τ})/B(Bover ¯→Dℓ^{-}νover ¯_{ℓ}) and R(D^{*})=B(Bover ¯→D^{*}τ^{-}νover ¯_{τ})/B(Bover ¯→D^{*}ℓ^{-}νover ...¯_{ℓ}), where ℓ denotes an electron or a muon, show a long-standing discrepancy with the standard model predictions, and might hint at a violation of lepton flavor universality. We report a new simultaneous measurement of R(D) and R(D^{*}), based on a data sample containing 772×10^{6} BBover ¯ events recorded at the ϒ(4S) resonance with the Belle detector at the KEKB e^{+}e^{-} collider. In this analysis the tag-side B meson is reconstructed in a semileptonic decay mode and the signal-side τ is reconstructed in a purely leptonic decay. The measured values are R(D)=0.307±0.037±0.016 and R(D^{*})=0.283±0.018±0.014, where the first uncertainties are statistical and the second are systematic. These results are in agreement with the standard model predictions within 0.2, 1.1, and 0.8 standard deviations for R(D), R(D^{*}), and their combination, respectively. This work constitutes the most precise measurements of R(D) and R(D^{*}) performed to date as well as the first result for R(D) based on a semileptonic tagging method.
We present a measurement of R_{K^{*}}, the branching fraction ratio B(B→K^{*}μ^{+}μ^{-})/B(B→K^{*}e^{+}e^{-}), for both charged and neutral B mesons. The ratio for the charged case R_{K^{*+}} is the ...first measurement ever performed. In addition, we report absolute branching fractions for the individual modes in bins of the squared dilepton invariant mass q^{2}. The analysis is based on a data sample of 711 fb^{-1}, containing 772×10^{6} BBover ¯ events, recorded at the ϒ(4S) resonance with the Belle detector at the KEKB asymmetric-energy e^{+}e^{-} collider. The obtained results are consistent with standard model expectations.
We present a measurement of angular observables and a test of lepton flavor universality in the B→K^{*}ℓ^{+}ℓ^{-} decay, where ℓ is either e or μ. The analysis is performed on a data sample ...corresponding to an integrated luminosity of 711 fb^{-1} containing 772×10^{6} BBover ¯ pairs, collected at the ϒ(4S) resonance with the Belle detector at the asymmetric-energy e^{+}e^{-} collider KEKB. The result is consistent with standard model (SM) expectations, where the largest discrepancy from a SM prediction is observed in the muon modes with a local significance of 2.6σ.
Abstract
We present a measurement of the Michel parameters of the $\tau$ lepton, $\bar{\eta}$ and $\xi\kappa$, in the radiative leptonic decay $\tau^- \rightarrow \ell^- \nu_{\tau} \bar{\nu}_{\ell} ...\gamma$ using 711 f$\mathrm{b}^{-1}$ of collision data collected with the Belle detector at the KEKB $e^+e^-$ collider. The Michel parameters are measured in an unbinned maximum likelihood fit to the kinematic distribution of $e^+e^-\rightarrow\tau^+\tau^-\rightarrow (\pi^+\pi^0 \bar{\nu}_\tau)(\ell^-\nu_{\tau}\bar{\nu}_{\ell}\gamma)$$(\ell=e$ or $\mu)$. The measured values of the Michel parameters are $\bar{\eta} = -1.3 \pm 1.5 \pm 0.8$ and $\xi\kappa = 0.5 \pm 0.4 \pm 0.2$, where the first error is statistical and the second is systematic. This is the first measurement of these parameters. These results are consistent with the Standard Model predictions within their uncertainties, and constrain the coupling constants of the generalized weak interaction.
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