Effect of ozone on the visible injury, nitrogen metabolism of rice leaves, and sugar and protein contents in rice grain was carried out by the open-top chamber. The results indicated that ozone ...stress caused obvious injury in rice leaves. The increase in ozone concentration had significant influence on the nitrate reductase activity in rice leaves. At the ozone concentration of 40, 80 and 120 nL L⁻¹, the nitrate reductase activities in rice leaves in the tillering stage, the jointing stage, the heading stage and milk stage were separately reduced by 25.3–86.3%, 57.4–97.8%, 91.0–99.3% and 89.5–96.7% compared with those in the control treatment. As ozone concentration increased, the contents of ammonium nitrogen and nitrate nitrogen in rice leaves were obviously reduced. Ozone stress also had an influence on the contents of sugar and protein in rice grain. The stress of high ozone concentration (120 nL L⁻¹) caused the starch content in grain to reduce by 15.8% than that in the control treatment, but total soluble sugars in grain was actually enhanced by 47.5% compared to that in the control treatment. The contents of albumin and glutenin in rice grain increased with increasing the ozone concentration, and prolamin and crude protein contents in rice grain increased only at the higher ozone concentration. Under ozone concentration of 120 nL L⁻¹, the contents of albumin, glutenin and crude protein in rice grain were increased respectively by 23.1%, 21.0% and 21.1% compared with those in the control treatment. The result suggested that ozone tress has an influence on nitrogen metabolism of rice leaves and grain quality.
The low cyle fatigue (LCF) behavior of an extruded AZ31B magnesium alloy with the fiber texture components was investigated under asymmetric loading at different total amplitudes. It was found that ...the alloy exhibits cyclic strain-hardening characteristic at the strain amplitudes ranging from 0.7% to 1.5%, however, the stress amplitude versus the number of reversals becomes almost constant as the strain amplitude reaches up to 2.0%. The cyclic hardening dominantly originates from tensile deformation when strain amplitude is below 1.5%, and its rate increases with the increment of strain amplitude; the hysteresis loop becomes asymmetrical in shape when the strain amplitude increases. From the deformed microstructures, it suggests that the cyclic plastic deformation at the low strain amplitudes is mainly associated with the dislocation slips while the twinning–detwinning is the dominant deformation way at high amplitudes. The asymmetry of the LCF behavior is discussed in terms of the difference of the plastic deformation mechanisms between compression and tension in cyclic reversals.
The tetraoctylammonium bromide (TOAB)-stabilized gold nanoparticles have been successfully fabricated. After an annealing of the as-synthesized nanoparticles at 300 °C for 30 min, the coalescence ...behavior of gold nanoparticles has been investigated using high-resolution transmission electron microscopy in detail. Two types of coalescence, one being an ordered combination of two or more particles in appropriate orientations through twinning, and the other being an ordered combination of two small particles with facets through a common lattice plane, have been observed.
We examine the nature of the unknown enhancement around 3 GeV observed by the BaBar Collaboration in the mpp¯ spectrum of the B¯0→pp¯D0 decay. Suspecting that the peak is a resonance, which can be ...neither identified as a charmonium state, such as ηc or J/ψ, nor classified as one of the light-flavor mesons, we conclude that it corresponds to a glueball fitted as X(3020) with (mX,ΓX)=(3020±8,107±30) MeV, which could be the first glueball state above 3 GeV. This state also appears in the mpp¯ spectrum of the B¯0→pp¯D⁎0 decay.
In this article, we studied the effect of magnetic interaction on β-relaxations of FexNi72−xSi4.8B19.2Nb4 (x = 0, 10, 30, 50, 72) metallic glasses (MGs). It is found that, with the substitution of Fe ...by Ni, the β-relaxation changes from a shoulder to an excess wing, suggesting an entropic effect on the suppression of β-relaxation. A peak caused by ferromagnetic transformation appears in the loss modulus curve of Fe30Ni42Si4.8B19.2Nb4 MG with suppressed β-relaxation, which is sensitive to stress and strain. In addition, the β-relaxation can be further varied by annealing under a transverse or longitudinal magnetic field. The results suggest that the entropy-suppressed β-relaxation in MGs can be affected by magnetic interaction that could help to improve their mechanical properties.
This study aimed to investigate the role of long-chain non-coding RNA CTBP1-AS in breast cancer progression and cell invasion as well as migration. Clinical data of breast cancer patients (N = 155) ...in our hospital was collected for further analysis. qRT-PCR was used to detect LncRNA CTBP1-AS expression levels in human normal breast epithelial cell (MCF-10A) and breast cancer cells (MCF-7, BT- 549, MDA-MB-231 and MDA-MB-435). LncRNA CTBP1-AS knock-down and overexpressed lentivirus vectors were constructed to transfect breast cancer cells. Colony formation assay was employed to detect cell proliferative abilities. Flow cytometry was performed to detect cell apoptosis ratio. Wound healing scratch assay was used to detect cell migration, and Transwell matrigel assay was used to detect cell invasion. Bioinformatics analysis was performed to predict the downstream targets of LncRNA CTBP1-AS, which were further validated by dual-luciferase reporter gene system. The results showed that LncRNA CTBP1-AS was aberrantly overexpressed in breast cancer tissues and breast cancer cells compared to the control group. Moreover, the expression levels of LncRNA CTBP1-AS were positively related with tumor size, histological grade and the expression levels of Ki-67 and Her2. Further analysis showed that LncRNA CTBP1-AS expression levels negatively correlated with patient survival time and clinical prognosis. Of note, overexpressed LncRNA CTBP1-AS promoted breast cancer cell proliferation and invasion as well as migration, and decreased cell apoptosis ratio. Bioinformatics analysis and dual-luciferase reporter gene system results validated that microRNA-940 was the downstream target of LncRNA CTBP1-AS. Interestingly, overexpressed microRNA-940 abrogated the effects of LncRNA CTBP1-AS on cell proliferation, apoptosis, and invasion. In conclusion, overexpressed LncRNA CTBP1- AS promoted breast cancer cell proliferation, invasion as well as migration, inhibited cell apoptosis and accelerated breast cancer development by sponging microRNA-940.
The lattice Boltzmann models, especially the pseudopotential models, have been developed to investigate multicomponent multiphase fluids in presence of phase change process. However, the ...interparticle force between different components causes compressibility error in the non-phase-change component. This restricts the model capability in quantitative analysis of the physical foaming process, such as expansion rate and decay time. In the present study, a multicomponent multiphase pseudopotential phase change model (the MMPPCM) is improved by introducing an effective mass form of high-pressure-difference multicomponent model in the non-phase-change component. The improved model is compared with the MMPPCM based on simulations of the phase change process of static and moving fluids, as well as the physical foaming process. Density variation of non-phase-change component and its effect on flow field characteristics are analyzed during the phase change process. Simulation results of physical foaming process lead to about 10% ~ 20% reduction of the compressibility error for the improved model as compared with the results of MMPPCM. The improved model also enhances the computational stability of phase change simulation of the static droplets.
Let-7 was one of the earliest discovered miRNAs and while it reportedly acts as a tumor suppressor in various solid tumors, its function in breast cancer has not been fully studied. Therefore, we ...examined let-7a and MAGE-A1 expression in breast tissues by qRT-PCR and found that let-7a expression significantly correlates with larger tumor size, higher histological grade (p<0.05) and is significantly lower in patients with Her-2-positive cancers and Ki-67 >14% (p=0.028 and p=0.023). MAGE-A1 expression incidence is 50.8% (33/65) and it inversely correlates with let-7a expression (p=0.008). let-7a inhibition of breast cancer cell proliferation, migration and invasion was also observed in in vitro cell culture experiments, and dual-luciferase reporter assays showed that melanoma-associated antigen A1 (MAGE-A1) was its target gene; the target comprised bases 451-457 of the 3'UTR region of the MAGE-A1 mRNA. RT-qPCR and Western blot analyses showed that let-7a inhibited MAGE-A1 expression at both the nucleic acid and protein levels. In our final co-transfection experiment, we targeted MAGE-A1 in a breast cancer cell line and observed that let-7a inhibited cell proliferation, migration and invasion. These combined results confirm that let-7a functions as a tumor suppressor by targeting MAGE-A1 in breast cancer and it therefore provides a novel target in breast cancer clinical treatment.
We study the three-body baryonic Formula omitted decays with M representing the Formula omitted or Formula omitted meson. Particularly, we predict that Formula omitted or Formula omitted, where the ...errors arise from the non-factorizable effects as well as the uncertainties in the Formula omitted and Formula omitted transition form factors, while the two different results are due to overall relative signs between the form factors, causing the constructive and destructive interference effects. For the corresponding baryonic Formula omitted decays, we find that Formula omitted or Formula omitted with the errors similar to those above. The decays in question are accessible to the experiments at BELLE and LHCb.