Many epidemiological studies revealed that shift work is associated with an increased risk of a number of pathologies, including cardiovascular diseases. An experimental model of shift work in rats ...has additionally been shown to recapitulate aspects of metabolic disorders observed in human shift workers, including increased fat content and impaired glucose tolerance, and used to demonstrate that restricting food consumption outside working hours prevents shift work-associated obesity and metabolic disturbance. However, the way distinct shift work parameters, such as type of work, quantity, and duration, affect cardiovascular function and the underlying mechanisms, remains poorly understood. Here, we used the rat as a model to characterize the effects of shift work in the heart and determine whether they can be modulated by restricting food intake during the normal active phase.
We show that experimental shift work reprograms the heart cycling transcriptome independently of food consumption. While phases of rhythmic gene expression are distributed across the 24-h day in control rats, they are clustered towards discrete times in shift workers. Additionally, preventing food intake during shift work affects the expression level of hundreds of genes in the heart, including genes encoding components of the extracellular matrix and inflammatory markers found in transcriptional signatures associated with pressure overload and cardiac hypertrophy. Consistent with this, the heart of shift worker rats not eating during work hours, but having access to food outside of shift work, exhibits increased collagen 1 deposition and displays increased infiltration by immune cells. While maintaining food access during shift work has less effects on gene expression, genes found in transcriptional signatures of cardiac hypertrophy remain affected, and the heart of shift worker rats exhibits fibrosis without inflammation.
Together, our findings unraveled differential effects of food consumption on remodeled transcriptional profiles of the heart in shift worker rats. They also provide insights into how shift work affects cardiac function and suggest that some interventions aiming at mitigating metabolic disorders in shift workers may have adverse effects on cardiovascular diseases.
Multi-port beam splitters are cornerstone devices for high-dimensional quantum information tasks, which can outperform the two-dimensional ones. Nonetheless, the fabrication of such devices has ...proven to be challenging with progress only recently achieved with the advent of integrated photonics. Here, we report on the production of high-quality
N
×
N
(with
N
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4
,
7
) multi-port beam splitters based on a new scheme for manipulating multi-core optical fibers. By exploring their compatibility with optical fiber components, we create four-dimensional quantum systems and implement the measurement-device-independent random number generation task with a programmable four-arm interferometer operating at a 2 MHz repetition rate. Due to the high visibilities observed, we surpass the one-bit limit of binary protocols and attain 1.23 bits of certified private randomness per experimental round. Our result demonstrates that fast switching, low loss, and high optical quality for high-dimensional quantum information can be simultaneously achieved with multi-core fiber technology.
Sleep has a major role in learning, memory consolidation, and metabolic function. Although it is known that sleep restriction increases the accumulation of amyloid β peptide (Aβ) and the risk to ...develop Alzheimer’s disease (AD), the mechanism behind these effects remains unknown. In this review, we discuss how chronic sleep restriction induces metabolic and cognitive impairments that could result in the development of AD in late life. Here, we integrate evidence regarding mechanisms whereby metabolic signaling becomes disturbed after short or chronic sleep restriction in the context of cognitive impairment, particularly in the accumulation of Aβ in the brain. We also discuss the role of the blood-brain barrier in sleep restriction with an emphasis on the transport of metabolic signals into the brain and Aβ clearance. This review presents the unexplored possibility that the alteration of peripheral metabolic signals induced by sleep restriction, especially insulin resistance, is responsible for cognitive deficit and, subsequently, implicated in AD development.
Timing of metabolic processes is crucial for balanced physiology; many studies have shown the deleterious effects of untimely food intake. The basis for this might be an interaction between the ...arcuate nucleus (ARC) as the main integration site for metabolic information and the suprachiasmatic nucleus (SCN) as the master clock. Here we show in male rats that the SCN influences ARC daily neuronal activity by imposing a daily rhythm on the α-MSH neurons with a peak in neuronal activity at the end of the dark phase. Bilateral SCN lesions showed a complete disappearance of ARC neuronal rhythms and unilateral SCN lesions showed a decreased activation in the ARC at the lesioned side. Moreover light exposure during the dark phase inhibited ARC and α-MSH neuronal activity. The daily inhibition of ARC neuronal activity occurred in light-dark conditions as well as in dark-dark conditions, demonstrating the inhibitory effect to be mediated by increased SCN (subjective) day neuronal activity. Injections into the SCN with the neuronal tracer cholera toxin B showed that α-MSH neurons receive direct projections from the SCN. The present study demonstrates that the SCN activates and possibly also inhibits depending on the moment of the circadian cycle ARC α-MSH neurons via direct neuronal input. The persistence of these activity patterns in fasted animals demonstrates that this SCN-ARC interaction is not necessarily satiety associated but may support physiological functions associated with changes in the sleep-wake cycle.
Diabetes mellitus type 2 (T2D) complications include brain damage which increases the risk of neurodegenerative diseases and dementia. An early manifestation of neurodegeneration is olfactory ...dysfunction (OD), which is also presented in diabetic patients. Previously, we demonstrated that OD correlates with IL-1β and miR-146a overexpression in the olfactory bulb (OB) on a T2D rodent model, suggesting the participation of inflammation on OD. Here, we found that OD persists on a long-term T2D condition after the downregulation of IL-1β. Remarkably, OD was associated with the increased expression of the dopaminergic neuronal marker tyrosine hydroxylase, ERK1/2 phosphorylation, and reduced neuronal activation on the OB of diabetic rats, suggesting the participation of the dopaminergic tone on the OD derived from T2D. Dopaminergic neurons are susceptible in neurodegenerative diseases such as Parkinson’s disease; therefore further studies must be performed to completely elucidate the participation of these neurons and ERK1/2 signaling on olfactory impairment.
The uninterruptible operation of grid-connected renewables under the occurrence of grid voltage sags is addressed in this work. This is achieved due to the incorporation of an enhanced control ...algorithm of a renewable source. The low-voltage ride-through algorithm was developed in accordance to the voltage profile introduced by IEC 61400-21 regarding grid voltage sags. To guarantee continuous operation of the renewable agent during voltage sags, not only instantaneous reactive power but also instantaneous active power under moderate voltage sags was injected to the utility grid fulfilling grid code requirements. A dual second-order generalized integrator frequency-locked loop synchronization algorithm was used to estimate the system’s frequency, together with the positive and the negative sequences of the three-phase utility grid voltages when unbalanced sags occurred. The current control was made in a stationary reference frame by using proportional-resonant regulators, and a DC voltage source was used to emulate the primary energy from any type of renewable system. The validation of the proposed control algorithm was conducted for a three-phase grid-connected renewable system with an apparent power of 500 kVA. The results from several experimental tests demonstrated the proper behavior of the enhanced algorithm.
•This paper gives a performance study of the Positive Sequence Detector plus a dqPLL.•A real-time digital simulation platform is used to validate the results.•With low-level harmonic pollution it is ...not a necessity to use a complicated synchronization algorithm.•A significant attenuation of the harmonic contamination is attained when the PSD block is used.•A good power factor can be obtained even though variations in frequency occur.
In a distributed generation (DG) system, several renewable agents are connected to the low-voltage 3-phase utility grid through an inverter which is used as power condition and must guarantee the higher efficiency of the renewable agent. To attain this level of efficiency, a unitary power factor (FP) between the inverter currents and the utility grid voltages is necessary, and a synchronization algorithm is needed for the perfect synchronization between the renewable agent and the 3-phase utility grid. Within this context, this paper gives a performance study of the Positive Sequence Detector plus a Synchronous Reference Frame Phase-Locked Loop (PSD+dqPLL) as the synchronization algorithm, evaluating its accuracy under different conditions and studying their advantages and drawbacks. A grid-connected photovoltaic system with a nominal power of 6kW is used so as to evaluate the behavior of the synchronization algorithm when the 3-phase utility grid is affected by some disturbances such as voltage unbalances, frequency variations and harmonic distortions. Firstly, several simulations with a disturbed 3-phase utility grid using MATLAB/SIMULINK from The MathWorks, Inc. are shown, and secondly, the previous tests are run in a Real-Time Digital Simulation (RTDS) platform in order to validate the obtained results with simulations.
The Transiting Exoplanet Survey Satellite (TESS) mission was designed to perform an all-sky search of planets around bright and nearby stars. Here we report the discovery of two sub-Neptunes orbiting ...around TOI 1062 (TIC 299799658), a V = 10.25 G9V star observed in the TESS Sectors 1, 13, 27, and 28. We use precise radial velocity observations from HARPS to confirm and characterize these two planets. TOI 1062b has a radius of 2.265(-0.091,+0.096) Rꚛ, a mass of 10.15 ± 0.8 Mꚛ, and an orbital period of 4.1130 ± 0.0015 days. The second planet is not transiting, has a minimum mass of 9.78(−1.18,+1.26) Mꚛ and is near the 2:1 mean motion resonance with the innermost planet with an orbital period of 7.972(−0.024,+0.018) days. We performed a dynamical analysis to explore the proximity of the system to this resonance, and to attempt further constraining the orbital parameters. The transiting planet has a mean density of 4.85(−0.74,+0.84) g/cu. cm and an analysis of its internal structure reveals that it is expected to have a small volatile envelope accounting for 0.35% of the mass at most. The star’s brightness and the proximity of the inner planet to what is know as the radius gap make it an interesting candidate for transmission spectroscopy, which could further constrain the composition and internal structure of TOI 1062b.
The circadian disruption in shift-workers is suggested to be a risk factor to develop overweight and metabolic dysfunction. The conflicting time signals given by shifted activity, shifted food intake ...and exposure to light at night occurring in the shift-worker are proposed to be the cause for the loss of internal synchrony and the consequent adverse effects on body weight and metabolism. Because food elicited signals have proven to be potent entraining signals for peripheral oscillations, here we review the findings from experimental models of shift-work and verify whether they provide evidence about the causal association between shifted feeding schedules, circadian disruption and altered metabolism. We found mainly four experimental models that mimic the conditions of shift-work: protocols of forced sleep deprivation, of forced activity during the normal rest phase, exposure to light at night and shifted food timing. A big variability in the intensity and duration of the protocols was observed, which led to a diversity of effects. A common result was the disruption of temporal patterns of activity; however, not all studies explored the temporal patterns of food intake. According to studies that evaluate time of food intake as an experimental model of shift-work and studies that evaluate shifted food consumption, time of food intake may be a determining factor for the loss of balance at the circadian and metabolic level.
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