Background
Gas-Coupled Laser Acoustic Detection (GCLAD) is a non-contact ultrasonic detection technique whose functioning relies on the deviation that a probe laser beam sustains when intersected by ...an acoustic wavefront propagating in a fluid. The maximum sensitivity of the technique is typically obtained when the ultrasound insists on an ample portion of the probe laser beam extension, but such a condition can be unfeasible in several non-destructive testing applications (as in case of limited accessibility to the component).
Objective
In the present work, a solution is provided enabling transformation of the GCLAD device in a point detector. This is based on the use of two mirrors for confining the laser beam in an area with limited width and depth, where reflections however maximize the portion of the probe laser beam subjected to ultrasonic oscillation.
Methods
The characteristics of the obtained GCLAD probe are thoroughly analysed by applying the device to the detection of surface acoustic waves, propagating on a metal bar and refracting into the air. Two different inspection configurations are considered, whose difference lies in the mutual orientation between laser beam and solid surface. The effect on the received signal amplitude of the number of beam reflections, the dimensions of the resulting device, and the bar axisimmetry is investigated in both configurations.
Results
The optimization of all the analysed standpoints enables obtaining a compact GCLAD probe that features the same signal amplitude of the non-compact alternative. To obtain maximum responsivity of the system, the number of reflections must be maximized, while the distance between the mirrors must be carefully set based on the employed inspection configuration and the eventual axisimmetry of the specimen. The devised GCLAD compact probe is capable of expanding the application range of the technique also to those cases in which the use of point detectors is desirable, without compromising the signal-to-noise ratio of the resulting acquisitions compared to the non-compact alternative.
Fluorescence in situ hybridization (FISH) and Hi-C methods are largely used to investigate the three-dimensional organization of the genome in the cell nucleus and are applied here to study the ...organization of genes (
,
,
,
,
) localized in the human 7q36.3 band. This region contains the
gene, which is normally not expressed in human lymphocytes beyond embryonic development. However, this homeobox gene is frequently activated in leukemic cells and its expression is associated with an altered gene positioning in the leukemia cell nuclei. In this study, we used FISH on 3D-preserved nuclei to investigate the nuclear positioning of
in the leukemia-derived cell line K562. Of the five copies of the
gene present in K562, four alleles were positioned in the nuclear periphery and only one in the nuclear interior. Using the Juicebox's Hi-C dataset, we identified five chromatin loops in the 7q36.3 band, with different extensions related to the size and orientation of the genes located here, and independent from their expression levels. We identified similar loops in 11 human and three mouse cell lines, showing that these loops are highly conserved in different human cell lines and during evolution. Moreover, the chromatin loop organization is well conserved also during neuronal cell differentiation, showing consistency in genomic organization of this region in development. In this report, we show that FISH and Hi-C are two different approaches that complement one another and together give complete information on the nuclear organization of specific chromosomal regions in different conditions, including cellular differentiation and genetic diseases.
Male breast cancer (MBC) is a rare disease compared with female BC and our current understanding regarding breast carcinogenesis in men has been largely extrapolated from the female counterpart. We ...focus on differences between the ethical issues related to male and female BC patients. A systematic literature search by using PubMed (http://www.ncbi.nlm.nih.gov/pubmed/), was carried out to provide a synopsis of the current research in the field of MBC genetics, epigenetics and ethics. Original articles and reviews published up to September 2012 were selected by using the following search key words to query the PubMed website: 'male breast cancer', 'male breast cancer and genetic susceptibility', 'male breast cancer and epigenetics', 'male breast cancer and methylation', 'male breast cancer and miRNA', 'male breast cancer and ethics'.
As in women, three classes of breast cancer genetic susceptibility (high, moderate, and low penetrance) are recognized in men. However, genes involved and their impact do not exactly overlap in female and male BC. Epigenetic alterations are currently scarcely investigated in MBC, however, the different methylation and miRNA expression profiles identified to date in female and male BCs suggest a potential role for epigenetic alterations as diagnostic biomarkers. Overall, much still needs to be learned about MBC and, because of its rarity, the main effort is to develop large consortia for moving forward in understanding MBC and improving the management of MBC patients on a perspective of gender medicine.
Laser-ultrasound technology is typically employed in case of non-destructive, non-contact inspection of mechanical components. In particular, low power laser sources (diodes) allow to contain ...implementation costs; on the other hand, identification of the ultrasonic peak is complex due to the low Signal-to-Noise Ratio (SNR), requiring the use of specific signal processing techniques. Features of the ultrasounds generated by the laser excitation, both in terms of frequencies and SNR, cannot be foreseen in advance, depending from the type of material and its thermo-elastic characteristics: it is thus fundamental to dispose of criteria to set in an optimal way the signal acquisition parameters to effectively apply a correct processing procedure and retrieve the useful information. In the work, surface R (Rayleigh) waves generated by a Continuous Wave (CW) low power laser are characterized, using a particular processing technique in the time domain. To identify the most influential input parameters on SNR, a Design Of Experiments (DOE) and a specific analysis are introduced: overall, the distance between source and receiver and the number of ensemble average applied before acquisition strongly affect SNR; the pulse duration results on the other hand influential at the same time on SNR and on the generated ultrasound frequency. Finally, analogies with longitudinal (L) waves generated by the same source are highlighted, allowing also for information on how to set up the investigation based on the type of wave and acquisition instruments employed.
The abundance of 26Al carries a special role in astrophysics, since it probes active nucleosynthesis in the Milky Way and constrains the Galactic core-collapse supernovae rate. It is estimated ...through the detection of the 1809 keV γ-line and from the superabundance of 26Mg in comparison with the most abundant Mg isotope (A = 24) in meteorites. For this reason, high precision is necessary also in the investigation of the stable 27Al and 24Mg isotopes. Moreover, these nuclei enter the so-called MgAl cycle, playing an important role in the production of Al and Mg. Recently, high-resolution stellar surveys have shown that the Mg–Al anticorrelation in red-giant stars in globular clusters may hide the existence of multiple stellar populations, and that the relative abundances of Mg isotopes may not be correlated with Al. The common thread running through these astrophysical scenarios is the 27Al(p,α)24Mg reaction, which is the main 27Al destruction channel and directly correlates its abundance with the 24Mg one. Since available reaction rates show large uncertainties owing to the vanishingly small cross section at astrophysical energies, we have applied the Trojan Horse Method to deduce the reaction rate with no need of extrapolation. The indirect measurement made it possible to assess the contribution of the 84 keV resonance and to lower upper limits on the strength of nearby resonances. In intermediate-mass AGB stars experiencing hot bottom burning, a sizeable increase in surface aluminum abundance is observed at the lowest masses, while 24Mg is essentially unaffected by the change in the reaction rate.
A double-phase argon Time Projection Chamber (TPC), with an active mass of 185 g, has been designed and constructed for the Recoil Directionality (ReD) experiment. The aim of the ReD project is to ...investigate the directional sensitivity of argon-based TPCs via columnar recombination to nuclear recoils in the energy range of interest (20–
200
keV
nr
) for direct dark matter searches. The key novel feature of the ReD TPC is a readout system based on cryogenic Silicon Photomultipliers (SiPMs), which are employed and operated continuously for the first time in an argon TPC. Over the course of 6 months, the ReD TPC was commissioned and characterised under various operating conditions using
γ
-ray and neutron sources, demonstrating remarkable stability of the optical sensors and reproducibility of the results. The scintillation gain and ionisation amplification of the TPC were measured to be
g
1
=
(
0.194
±
0.013
)
photoelectrons/photon and
g
2
=
(
20.0
±
0.9
)
photoelectrons/electron, respectively. The ratio of the ionisation to scintillation signals (S2/S1), instrumental for the positive identification of a candidate directional signal induced by WIMPs, has been investigated for both nuclear and electron recoils. At a drift field of 183 V/cm, an S2/S1 dispersion of 12% was measured for nuclear recoils of approximately 60–
90
keV
nr
, as compared to 18% for electron recoils depositing 60 keV of energy. The detector performance reported here meets the requirements needed to achieve the principal scientific goals of the ReD experiment in the search for a directional effect due to columnar recombination. A phenomenological parameterisation of the recombination probability in LAr is presented and employed for modeling the dependence of scintillation quenching and charge yield on the drift field for electron recoils between 50–500 keV and fields up to 1000 V/cm.
Crucial information on nova nucleosynthesis can be potentially inferred from γ-ray signals powered by 18F decay. Therefore, the reaction network producing and destroying this radioactive isotope has ...been extensively studied in the last years. Among those reactions, the 18F(p, )15O cross-section has been measured by means of several dedicated experiments, both using direct and indirect methods. The presence of interfering resonances in the energy region of astrophysical interest has been reported by many authors including the recent applications of the Trojan Horse Method. In this work, we evaluate what changes are introduced by the Trojan Horse data in the 18F(p, )15O astrophysical factor recommended in a recent R-matrix analysis, accounting for existing direct and indirect measurements. Then the updated reaction rate is calculated and parameterized and implications of the new results on nova nucleosynthesis are thoroughly discussed.
Low-power laser generated ultrasounds are lately gaining importance in the research world, thanks to the possibility of investigating a mechanical component structural integrity through a non-contact ...and Non-Destructive Testing (NDT) procedure. The ultrasounds are, however, very low in amplitude, making it necessary to use pre-processing and post-processing operations on the signals to detect them. The cross-correlation technique is used in this work, meaning that a random signal must be used as laser input. For this purpose, a highly random and simple-to-create code called T sequence, capable of enhancing the ultrasound detectability, is introduced (not previously available at the state of the art). Several important parameters which characterize the T sequence can influence the process: the number of pulses Npulses, the pulse duration δ and the distance between pulses dpulses. A Finite Element FE model of a 3 mm steel disk has been initially developed to analytically study the longitudinal ultrasound generation mechanism and the obtainable outputs. Later, experimental tests have shown that the T sequence is highly flexible for ultrasound detection purposes, making it optimal to use high Npulses and δ but low dpulses. In the end, apart from describing all phenomena that arise in the low-power laser generation process, the results of this study are also important for setting up an effective NDT procedure using this technology.
•A new indirect measurement of the 27Al(p,α)24Mg has been carried out down to astrophysical energies.•The strength of the 84.3 keV resonance has been measured, and more stringent upper limits of ...nearby resonances have been set.•A new recommended reaction rate has been established, leading to a factor of 3 lower 27Al destruction rate.•Preliminary nucleosynthesis calculations have shown ∼30% changes in 27Al and 24Mg abundances for intermediate mass stars.
The 26Al abundance holds a special role in present-day astrophysics, since it is a probe of active nucleosynthesis in the Galaxy and a valuable constraint of Galactic core-collapse supernovae rate. It is estimated through the detection of the 1809-keV γ-line of the daughter 26Mg and from the superabundance of 26Mg in comparison with the most abundant 24Mg isotope in meteorites. Accurate knowledge of the reaction rates involving 26Al, its stable counterpart 27Al and 24Mg is then mandatory. Moreover, these nuclei enter the MgAl cycle playing an important role in the production of Al and Mg isotopes. Recently, high-resolution stellar surveys have shown that the Mg-Al anti-correlation in red giants of globular clusters may hide the existence of multiple stellar populations, and that the relative abundances of Mg isotopes may not show correlation with Al.
The common thread running through these astrophysical scenarios is the 27Al(p,α)24Mg reaction, which is the main 27Al destruction channel and directly correlates its abundance with the 24Mg one. Since available reaction rates show an order of magnitude uncertainty owing to the vanishingly small cross section at astrophysical energies, we have applied the Trojan Horse Method to deduce the reaction rate with no need of extrapolation. The indirect measurement made it possible to assess the contribution of the 84-keV resonance and to lower the upper limits on the strength of nearby resonances, with potential important impact for astrophysics. In particular, modifications in the 27Al and 24Mg abundances up to ∼30% are predicted for intermediate mass stars.
Carbon burning powers scenarios that influence the fate of stars, such as the late evolutionary stages of massive stars 1 (exceeding eight solar masses) and superbursts from accreting neutron ...stars2,3. It proceeds through the 12C + 12C fusion reactions that produce an alpha particle and neon-20 or a proton and sodium-23-that is, 12C(12C, α)20Ne and 12C(12C, p)23Na-at temperatures greater than 0.4 × 109 kelvin, corresponding to astrophysical energies exceeding a megaelectronvolt, at which such nuclear reactions are more likely to occur in stars. The cross-sections 4 for those carbon fusion reactions (probabilities that are required to calculate the rate of the reactions) have hitherto not been measured at the Gamow peaks 4 below 2 megaelectronvolts because of exponential suppression arising from the Coulomb barrier. The reference rate 5 at temperatures below 1.2 × 109 kelvin relies on extrapolations that ignore the effects of possible low-lying resonances. Here we report the measurement of the 12C(12C, α0,1)20Ne and 12C(12C, p0,1)23Na reaction rates (where the subscripts 0 and 1 stand for the ground and first excited states of 20Ne and 23Na, respectively) at centre-of-mass energies from 2.7 to 0.8 megaelectronvolts using the Trojan Horse method6,7 and the deuteron in 14N. The cross-sections deduced exhibit several resonances that are responsible for very large increases of the reaction rate at relevant temperatures. In particular, around 5 × 108 kelvin, the reaction rate is boosted to more than 25 times larger than the reference value 5 . This finding may have implications such as lowering the temperatures and densities 8 required for the ignition of carbon burning in massive stars and decreasing the superburst ignition depth in accreting neutron stars to reconcile observations with theoretical models 3 .
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