Two-dimensional hafnium-based semiconductors and their heterostructures with native oxides have been shown unique physical properties and potential electronic and optoelectronic applications. ...However, the scalable synthesis methods for ultrathin layered hafnium-based semiconductor laterally epitaxy growth and its heterostructure are still restricted, also for the understanding of its formation mechanism. Herein, we report the stable sublimation of alkali halide vapor assisted synthesis strategy for high-quality 2D HfSe
2
nanosheets via chemical vapor deposition. Single-crystalline ultrathin 2D HfSe
2
nanosheets were systematically grown by tuning the growth parameters, reaching the lateral size of 6–40 µm and the thickness down to 4.5 nm. The scalable amorphous HfO
2
and HfSe
2
heterostructures were achieved by the controllable oxidation, which benefited from the approximate zero Gibbs free energy of unstable 2D HfSe
2
templates. The crystal structure, elemental, and time dependent Raman characterization were carried out to understand surface precipitated Se atoms and the formation of amorphous Hf–O bonds, confirming the slow surface oxidation and lattice incorporation of oxygen atoms. The relatively smooth surface roughness and electrical potential change of HfO
2
–HfSe
2
heterostructures indicate the excellent interface quality, which helps obtain the high performance memristor with high on/off ratio of 10
5
and long retention period over 9000 s. Our work introduces a new vapor catalysts strategy for the synthesis of lateral 2D HfSe
2
nanosheets, also providing the scalable oxidation of the Hf-based heterostructures for 2D electronic devices.
Niobium‐based oxides with wide bandgap and high dielectric constant show great potential in the applications of electronic and optoelectronic devices. Herein, the quasi‐van der Waals epitaxial growth ...of 2D α‐Nb2O5 nanosheets is reported, in which the growth promoter of sulfur and alkali halides has been utilized to catalyze the ultrathin 2D growth. The relatively low Gibbs free energy of α‐Nb2O5 nanosheets can drive the ultrathin growth down to 30 nm on the c‐Al2O3 substrate by the transformation of T‐Nb2O5 powder sources without any doping effects, demonstrating the diverse α‐Nb2O5 nanostructure morphologies. The as‐grown α‐Nb2O5 nanosheets are characterized with high crystalline quality and specific dominated growth plane, indicating the uniform dielectric properties. The metal–insulator–metal capacitor has confirmed the α‐Nb2O5 nanosheet with a high dielectric constant over 40. The dual promoters’ growth design strategy provides a universal synthesis method for the 2D nonlayered dielectric materials.
A new growth strategy has been created by designing growth promoter of sulfur and alkali halide, achieving ultrathin α‐Nb2O5 2D nanosheets with single‐crystalline quality and large lateral size. The high dielectric constant over 40 is shown by the metal–insulator–metal capacitator measurements. Herein, a special growth technique is provided for the synthesis of ultrathin 2D nonlayered dielectrics.
Niobium‐based oxides with wide bandgap and high dielectric constant show great potential in the applications of electronic and optoelectronic devices. Herein, the quasi‐van der Waals epitaxial growth ...of 2D α‐Nb 2 O 5 nanosheets is reported, in which the growth promoter of sulfur and alkali halides has been utilized to catalyze the ultrathin 2D growth. The relatively low Gibbs free energy of α‐Nb 2 O 5 nanosheets can drive the ultrathin growth down to 30 nm on the c‐Al 2 O 3 substrate by the transformation of T‐Nb 2 O 5 powder sources without any doping effects, demonstrating the diverse α‐Nb 2 O 5 nanostructure morphologies. The as‐grown α‐Nb 2 O 5 nanosheets are characterized with high crystalline quality and specific dominated growth plane, indicating the uniform dielectric properties. The metal–insulator–metal capacitor has confirmed the α‐Nb 2 O 5 nanosheet with a high dielectric constant over 40. The dual promoters’ growth design strategy provides a universal synthesis method for the 2D nonlayered dielectric materials.
Autoimmune diseases are characterized by an imbalance between regulatory T cells and effector T-cell subsets, such as Thl and Th17 cells. Studies have confirmed that natural CD4＋Foxp3＋ Tregs were ...unstable and dysfunctional in the presence of pro-inflammatory cytokines. In the current study, human CD39hi Tregs and CD39low Tregs were sorted from Tregs in vitro after 7 days of expansion. The functions of both Treg subsets were investigated under inflammatory conditions in vitro and in vivo. In the presence of IL-1β and IL-6, cultured CD4＋CD39hi Tregs maintained stable forkhead box protein 3 expression, whereas CD4＋CD39low Tregs lost Foxp3 expression and trans-differentiated into Thl or Th17 cells. Decreased IL-1βR and IL-6R expression on the CD39hi Tregs was the primary mechanism responsible for Treg stability. In addition, reduced activation of downstream molecules, such as STAT1 and STAT3, through the modulation of CpG demethylation played an important role. Finally, human CD4＋CD39hi Tregs but not CD4＋CD39low Tregs protected against xenograft versus host disease in model mice. These results strongly implied the physiological importance of CD39 expression and suggested that manipulation of CD39hi Tregs might represent a novel strategy for the treatment of autoimmune diseases.
The abuse of traditional antibiotics has caused a series of health problems including antimicrobial resistance, which threatens human health. Therefore, searching for broad sources of antimicrobial ...agents and developing multidimensional strategies to combat bacterial infections are urgent. Here, we reported two natural self-assembling modes between berberine (BBR) and flavonoid glycosides: nanoparticles (NPs) and nanofibers (NFs), which were both mainly governed by electrostatic and hydrophobic interactions. These two nanostructures exhibited different antibacterial properties from BBR. NPs showed significantly enhanced bacteriostatic activity, whereas NFs displayed a much weaker effect than BBR. The distinguishing properties can be attributed to the different spatial configurations and self-assembly processes of NPs and NFs. Flavonoid glycosides and BBR first formed a one-dimensional complex unit and subsequently self-assembled into three-dimensional nanostructures. With the hydrophilic glucuronic acid toward the outside, NPs exhibited stronger affinity to bacteria, thereby inducing the collapse of the bacteria population and the decrease in biofilm. In addition, in vitro hemolysis tests, cytotoxicity tests, and in vivo zebrafish toxicity evaluation showed that the obtained self-assemblies had good biocompatibility. This supramolecular self-assembly strategy can be applied to construct other nanoscale antibacterial drugs and thus provides weapons for the development of self-delivering drugs in bacterial infection treatment.
The application of nanotechnology for antimicrobial delivery has capacity to improve antibacterial efficacy. Currently, the usage of various inorganic and organic carriers, such as metal ions, ...nano-silicon and surfactants, might increase the potential toxicity of nanoparticles and make their clinical transformation more difficult. Herein, a nano-delivery system was constructed by direct self-assembly of antibacterial phytochemicals (berberine and rhein) originated from traditional Chinese medicine Coptis chinensis Franch. and Rheum palmatum L., respectively. Combining X-ray single crystal diffraction, nuclear magnetic resonance and other spectra characterizations, the stacked structure of nanoparticles was profoundly demonstrated. Briefly, rhein acted as the layered backbone and berberine embedded in it. In vitro bacteriostasis experiment showed the minimum bactericidal concentration of nanoparticles was 0.1 μmol/mL, which was lower than that of berberine and rhein. The results of confocal laser scanning microscope, biofilm quantitive assay and scanning electron microscopy indicated that nanoparticles had strong inhibitory effects on Staphylococcus aureus biofilm. More importantly, transmission electron microscopy and mass spectra indicated the further bacteriostatic mechanism of nanoparticles. Meanwhile, the nanoparticles had well biocompatibility and safety. Current study will open up new prospect that the design of self-assemblies between active phytochemicals can be originated from traditional Chinese medicine combination.
Ber-Rhe nanoparticles (NPs) were formed by direct self-assembly of Ber and Rhe. Combining X-ray single crystal diffraction and spectral analyses, the self-assembly mechanism of NPs had been demonstrated. Meanwhile, Ber–Rhe NPs demonstrated the inhibitory effect against Staphylococcus aureus by destroying the cell wall & membrane and cleaning the biofilm. Display omitted
Inferring 3D human motion is fundamental in many applications, including understanding human activity and analyzing one's intention. While many fruitful efforts have been made to human motion ...prediction, most approaches focus on pose-driven prediction and inferring human motion in isolation from the contextual environment, thus leaving the body location movement in the scene behind. However, real-world human movements are goal-directed and highly influenced by the spatial layout of their surrounding scenes. In this paper, instead of planning future human motion in a "dark" room, we propose a Multi-Condition Latent Diffusion network (MCLD) that reformulates the human motion prediction task as a multi-condition joint inference problem based on the given historical 3D body motion and the current 3D scene contexts. Specifically, instead of directly modeling joint distribution over the raw motion sequences, MCLD performs a conditional diffusion process within the latent embedding space, characterizing the cross-modal mapping from the past body movement and current scene context condition embeddings to the future human motion embedding. Extensive experiments on large-scale human motion prediction datasets demonstrate that our MCLD achieves significant improvements over the state-of-the-art methods on both realistic and diverse predictions.
S. aureus is resistant to various first-line antibiotics, and seeking multifarious strategies aimed at effective control of antibiotic-resistant behavior is urgently needed. Here, we report a ...two-component directed self-assembly mode: the phytochemicals berberine and cinnamic acid can directly self-assemble into nanoparticles (NPs) displaying good bacteriostastic activity. Compared with several first-line antibiotics, the obtained nanostructures have a better inhibitory effect on multidrug-resistant S. aureus (MRSA) and stronger ability for biofilm removal. These qualities are attributed to the fact that organic assemblies can first spontaneously adhere to the surface of the bacteria, infiltrate into the cell, and then lead to converging attack against MRSA; thereafter, multipath bactericidal mechanisms of NPs on MRSA are found by both transcriptomic analysis and quantitative Polymerase Chain Reaction analysis. Moreover, when combined with spectral data and single crystal X-ray diffraction, the NPs’ self-assembly mechanism governed by hydrogen bonds and π–π stacking interactions is clearly elucidated. These non-covalent interactions induce the NPs’ formation of butterfly-like one-dimensional self-assembled units and finally layered three-dimensional spatial configuration. In addition, biocompatibility tests show that the NPs are nonhemolytic with little toxicity in vitro and in vivo. This directed self-assembly mode can offer a new perspective toward the design of biocompatible antimicrobial nanomedicines for clinical translation.
Panel‐based methods are commonly employed for the analysis of novel gene fusions in precision diagnostics and new drug development in cancer. However, these methods are constrained by limitations in ...ligation yield and the enrichment of novel gene fusions with low variant allele frequencies. In this study, we conducted a pioneering investigation into the stability of double‐stranded adapter DNA, resulting in improved ligation yield and enhanced conversion efficiency. Additionally, we implemented blocker displacement amplification, achieving a remarkable 7‐fold enrichment of novel gene fusions. Leveraging the pre‐enrichment achieved with this approach, we successfully applied it to Nanopore sequencing, enabling ultra‐fast analysis of novel gene fusions within one hour with high sensitivity. This method offers a robust and remarkably sensitive mean of analyzing novel gene fusions, promising the discovery of pivotal biomarkers that can significantly improve cancer diagnostics and the development of new therapeutic strategies.
The state of double‐strand stem in adapter imposes effect on ligation strategy and reaction equilibrium. We conducted a pioneering investigation into the stability of double‐stranded adapter DNA and cascaded it with blocker displacement amplification technology, enabling improved ligation yield with enhanced conversion efficiency and selective enrichment in mutations with low variant allele frequency.
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