Demographic data are important to wildlife managers to gauge population health, to allow populations to be utilised sustainably, and to inform conservation efforts. We analysed published demographic ...data on the world's wildfowl to examine taxonomic and geographic biases in study, and to identify gaps in knowledge. Wildfowl (order: Anseriformes) are a comparatively well studied bird group which includes 169 species of duck, goose and swan. In all, 1,586 wildfowl research papers published between 1911 and 2010 were found using Web of Knowledge (WoK) and Google Scholar. Over half of the research output involved just 15 species from seven genera. Research output was strongly biased towards 'high income' countries, common wildfowl species, and measures of productivity, rather than survival and movement patterns. There were significantly fewer demographic data for the world's 31 threatened wildfowl species than for non-threatened species. Since 1994, the volume of demographic work on threatened species has increased more than for non-threatened species, but still makes up only 2.7% of total research output. As an aid to research prioritisation, a metric was created to reflect demographic knowledge gaps for each species related to research output for the species, its threat status, and availability of potentially useful surrogate data from congeneric species. According to the metric, the 25 highest priority species include thirteen threatened taxa and nine species each from Asia and South America, and six from Africa.
•Fe, Ni, Co, Mn and Mo concentrations were found to be deficient in the wastewater.•Addition of these trace elements improved the AD of slaughterhouse wastewater.•Higher OLR and lower HRT were ...achieved when trace elements were applied.•Improved process stability resulted in more biogas generated compared to controls.
In this study, anaerobic digestion of slaughterhouse wastewater with the addition of trace elements was monitored for biogas quantity, quality and process stability using CSTR digesters operated at mesophilic temperature. The determination of trace element concentrations was shown to be deficient in Fe, Ni, Co, Mn and Mo compared to recommendations given in the literature. Addition of these trace elements resulted in enhanced degradation efficiency, higher biogas production and improved process stability. Higher organic loading rates and lower hydraulic retention times were achieved in comparison to the control digesters. A critical accumulation of volatile fatty acids was observed at an organic loading rate of 1.82 g L–1 d–1 in the control compared to 2.36 g L–1 d–1 in the digesters with trace element addition. The improved process stability was evident in the final weeks of experimentation, in which control reactors produced 84% less biogas per day compared to the reactors containing trace elements.
Impaired apoptosis is now recognized to be central to tumor development. Bcl2, activated by chromosomal translocation in human follicular lymphoma, promotes oncogenesis by inhibiting apoptosis. Bim, ...a distant proapoptotic relative, is emerging as a major physiologic antagonist of Bcl2. Here, we show that loss of Bim is oncogenic. Bim protein levels were elevated in the apoptosis-prone B lymphoid cells of Eμ-Myc-transgenic mice, and Bim-mutant Eμ-Myc mice had increased numbers of IgM-bearing B cells. Eμ-Myc-expressing B lymphoid cells deficient in Bim were refractory to apoptosis induced in vitro by cytokine deprivation or antigen receptor cross-linking. Thus, Bim is induced by Myc in B cells and mediates apoptosis. Remarkably, inactivation of even a single allele of Bim accelerated Myc-induced development of tumors, particularly acute B cell leukemia. None of the primary tumors from Bim+/- Eμ-Myc mice displayed loss of the second allele of Bim. These findings indicate that Bim is a tumor suppressor, at least in B lymphocytes, and is haploinsufficient. Whereas the p19Arf/p53 pathway is frequently mutated in tumors arising in Bim+/+ Eμ-Myc mice, it was unaffected in most Bim-deficient tumors, indicating that Bim reduction is an effective alternative to loss of p53 function.
Percussive technology continues to play an increasingly important role in understanding the evolution of tool use. Comparing the archaeological record with extractive foraging behaviors in nonhuman ...primates has focused on percussive implements as a key to investigating the origins of lithic technology. Despite this, archaeological approaches towards percussive tools have been obscured by a lack of standardized methodologies. Central to this issue have been the use of qualitative, non-diagnostic techniques to identify percussive tools from archaeological contexts. Here we describe a new morphometric method for distinguishing anthropogenically-generated damage patterns on percussive tools from naturally damaged river cobbles. We employ a geomatic approach through the use of three-dimensional scanning and geographical information systems software to statistically quantify the identification process in percussive technology research. This will strengthen current technological analyses of percussive tools in archaeological frameworks and open new avenues for translating behavioral inferences of early hominins from percussive damage patterns.
Abstract
Negative feedback is known to enable biological and man-made systems to perform reliably in the face of uncertainties and disturbances. To date, synthetic biological feedback circuits have ...primarily relied upon protein-based, transcriptional regulation to control circuit output. Small RNAs (sRNAs) are non-coding RNA molecules that can inhibit translation of target messenger RNAs (mRNAs). In this work, we modelled, built and validated two synthetic negative feedback circuits that use rationally-designed sRNAs for the first time. The first circuit builds upon the well characterised tet-based autorepressor, incorporating an externally-inducible sRNA to tune the effective feedback strength. This allows more precise fine-tuning of the circuit output in contrast to the sigmoidal, steep input-output response of the autorepressor alone. In the second circuit, the output is a transcription factor that induces expression of an sRNA, which inhibits translation of the mRNA encoding the output, creating direct, closed-loop, negative feedback. Analysis of the noise profiles of both circuits showed that the use of sRNAs did not result in large increases in noise. Stochastic and deterministic modelling of both circuits agreed well with experimental data. Finally, simulations using fitted parameters allowed dynamic attributes of each circuit such as response time and disturbance rejection to be investigated.
To determine, in patients undergoing joint preservation procedures, whether the Forgotten Joint Score (FJS) compares favorably with legacy measures.
Medical databases (including PubMed/MEDLINE and ...Embase databases) were queried for publications with the terms "Forgotten Joint Score" and "hip," "knee," "arthroscopy," or "ACL." Fourteen studies met the inclusion criteria. Methodologic quality was assessed through the Consensus-based Standards for the Selection of Health Measurement Instruments (COSMIN) checklist, and psychometric data were evaluated for ceiling or floor effects, convergent validity, internal consistency, reliability, responsiveness, measurement invariance, and measurement error by 2 fellowship-trained orthopaedic surgeons (B.D.K. and W.T.H.).
Data were collected from 14 studies using the FJS after joint-preserving procedures in 911 patients (959 joints). Four studies reported strong internal consistency with an average Cronbach α of 0.92. Two studies reported responsiveness with an effect size ranging from 0.6 to 1.16. One study reported reproducibility with an interclass correlation coefficient of 0.9 (95% confidence interval, 0.8-0.9). One study reported measurement error with an minimum detectable change (MDC)
of 32% and MDC
of 4.5%. Studies reported moderate to very strong convergent validity across legacy measures for hip and knee preservation surgery. Ceiling effects were favorable compared with many legacy scores for hip and knee preservation. Three studies reported the minimal clinically important difference whereas 1 study reported the patient acceptable symptomatic state for the FJS.
The FJS is a methodologically sound outcome measure used to evaluate patient outcomes after hip and knee preservation surgery with overall low ceiling effects compared with legacy measures.
Level IV, systematic review of Level III and IV studies.
The asteroid lightcurve database Warner, Brian D.; Harris, Alan W.; Pravec, Petr
Icarus (New York, N.Y. 1962),
07/2009, Letnik:
202, Številka:
1
Journal Article
Recenzirano
The compilation of a central database for asteroid lightcurve data, i.e., rotation rate and amplitude along with ancillary information such as diameter and albedo (known or estimated), taxonomic ...class, etc., has been important to statistical studies for several decades. Having such a compilation saves the researcher hours of effort combing through any number of journals, some obvious and some not, to check on prior research. Harris has been compiling such data in the Asteroid Lightcurve Database (LCDB) for more than 25 years with Warner and Pravec assisting the past several years. The main data included in the LCDB are lightcurve rotation periods and amplitudes, color indices, H–G parameters, diameters (actual or derived), basic binary asteroid parameters, and spin axis and shape models. As time permits we are reviewing existing entries to enter data not previously recorded (e.g., phase angle data). As of 2008 December, data for 3741 asteroids based on more than 10650 separate detail records derived from entries in various journals were included in the LCDB. Of those 3741 asteroids, approximately 3100 have data of sufficient quality for statistical analysis, including 7 that have “dual citizenship” – meaning that they have (or had) asteroid designations as well comet designations. Here we present a discussion of the nature of LCDB data, i.e., which values are actually measured and which are derived. For derived data, we give our justification for specific values. We also present some analysis based on the LCDB data, including new default albedo (pV) and phase slope parameter (G) values for the primary taxonomic classes and a review of the frequency–diameter distribution of all asteroids as well as some selected subsets. The most recent version of data used in this analysis is available for download from the Collaborative Asteroid Lightcurve Link (CALL) site at http://www.MinorPlanetObserver.com/astlc/default.htm. Other data sets, some only subsets of the full LCDB, are available in the Ephemeris of Minor Planets, The Planetary Data System, and the Minor Planet Center web site.
WormBase (www.wormbase.org) is a central repository for research data on the biology, genetics and genomics of Caenorhabditis elegans and other nematodes. The project has evolved from its original ...remit to collect and integrate all data for a single species, and now extends to numerous nematodes, ranging from evolutionary comparators of C. elegans to parasitic species that threaten plant, animal and human health. Research activity using C. elegans as a model system is as vibrant as ever, and we have created new tools for community curation in response to the ever-increasing volume and complexity of data. To better allow users to navigate their way through these data, we have made a number of improvements to our main website, including new tools for browsing genomic features and ontology annotations. Finally, we have developed a new portal for parasitic worm genomes. WormBase ParaSite (parasite.wormbase.org) contains all publicly available nematode and platyhelminth annotated genome sequences, and is designed specifically to support helminth genomic research.
Jupiter-family comets (JFCs) are the evolutionary products of trans-Neptunian objects (TNOs) that evolve through the giant planet region as Centaurs and into the inner solar system. Through numerical ...orbital evolution calculations following a large number of TNO test particles that enter the Centaur population, we have identified a short-lived dynamical Gateway, a temporary low-eccentricity region exterior to Jupiter through which the majority of JFCs pass. We apply an observationally based size distribution function to the known Centaur population and obtain an estimated Gateway region population. We then apply an empirical fading law to the rate of incoming JFCs implied by the the Gateway region residence times. Our derived estimates are consistent with observed population numbers for the JFC and Gateway populations. Currently, the most notable occupant of the Gateway region is 29P/Schwassmann-Wachmann 1 (SW1), a highly active, regularly outbursting Centaur. SW1's present-day, very-low-eccentricity orbit was established after a 1975 Jupiter conjunction and will persist until a 2038 Jupiter conjunction doubles its eccentricity and pushes its semimajor axis out to its current aphelion. Subsequent evolution will likely drive SW1's orbit out of the Gateway region, perhaps becoming one of the largest JFCs in recorded history. The JFC Gateway region coincides with a heliocentric distance range where the activity of observed cometary bodies increases significantly. SW1's activity may be typical of the early evolutionary processing experienced by most JFCs. Thus, the Gateway region, and its most notable occupant SW1, are critical to both the dynamical and physical transition between Centaurs and JFCs.
The accurate description of ancestry is essential to interpret, access, and integrate human genomics data, and to ensure that these benefit individuals from all ancestral backgrounds. However, there ...are no established guidelines for the representation of ancestry information. Here we describe a framework for the accurate and standardized description of sample ancestry, and validate it by application to the NHGRI-EBI GWAS Catalog. We confirm known biases and gaps in diversity, and find that African and Hispanic or Latin American ancestry populations contribute a disproportionately high number of associations. It is our hope that widespread adoption of this framework will lead to improved analysis, interpretation, and integration of human genomics data.
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