Mercury contamination is a global issue because mercury concentrations in aquatic systems are influenced by both natural and anthropogenic pathways. Here, liver and muscle total mercury (THg) ...concentrations in black crappie
Pomoxis nigromaculatus
from three boreal lakes in southeastern Manitoba, Canada, were related to age, morphology and physiological traits to better understand the dynamics of mercury accumulation in an introduced generalist fish species. These THg concentrations were then compared to black crappie mercury concentrations in other Canadian water bodies and to mercury concentrations in other freshwater fishes in southeastern Manitoba. Age and size had strong positive correlations (
P
< 0.001,
r
≥ 0.60) with muscle mercury concentrations. No evidence of acute point source contamination or physiological impairment in black crappie was found in the study area. Analysis of liver THg revealed the possible impacts of seasonal and ontogenetic differences in diet on exposure. Furthermore, THg analysis of liver and muscle tissue showed how generalist foraging may curb the progressively greater mercury exposure and resultant physiological consequences expected from ontogenetic diet shifts in black crappie. Although there appeared to be temporally varied levels of mercury exposure (i.e., liver THg) by sex, there was no sex effect observed in long-term mercury accumulation in the muscle. Black crappie bioaccumulated less mercury at age than primary piscivore species in the region. These results will help foster a better understanding of mercury biomagnification within a region impacted by legacy mercury.
Introduction
Mitochondrial dysfunction is observed in Alzheimer’s disease (AD). Altered mitochondrial respiration, cytochrome oxidase (COX) Vmax, and mitophagy are observed in human subjects and ...animal models of AD. Models derived from induced pluripotent stem cells (iPSCs) may not recapitulate these phenotypes after reprogramming from differentiated adult cells.
Methods
We examined mitochondrial function across iPSC derived models including cerebral organoids, forebrain neurons, and astrocytes. iPSCs were reprogrammed from fibroblasts either from the University of Kansas Alzheimer’s Disease Research Center (KU ADRC) cohort or purchased from WiCell. A total of four non-demented and four sporadic AD iPSC lines were examined. Models were subjected to mitochondrial respiration analysis using Seahorse XF technology, spectrophotometric cytochrome oxidase (COX) Vmax assays, fluorescent assays to determine mitochondrial mass, mitochondrial membrane potential, calcium, mitochondrial dynamics, and mitophagy levels. AD pathological hallmarks were also measured.
Results
iPSC derived neurons and cerebral organoids showed reduced COX Vmax in AD subjects with more profound defects in the female cohort. These results were not observed in astrocytes. iPSC derived neurons and astrocytes from AD subjects had reduced mitochondrial respiration parameters with increased glycolytic flux. iPSC derived neurons and astrocytes from AD subjects showed sex dependent effects on mitochondrial membrane potential, mitochondrial superoxide production, and mitochondrial calcium. iPSC derived neurons from AD subjects had reduced mitochondrial localization in lysosomes with sex dependent effects on mitochondrial mass, while iPSC derived astrocytes from female AD subjects had increased mitochondrial localization to lysosomes. Both iPSC derived neurons and astrocytes from AD subjects showed altered mitochondrial dynamics. iPSC derived neurons had increased secreted Aβ, and sex dependent effects on total APP protein expression. iPSC derived astrocytes showed sex dependent changes in GFAP expression in AD derived cells.
Conclusion
Overall, iPSC derived models from AD subjects show mitochondrial phenotypes and AD pathological hallmarks in a cell type and sex dependent manner. These results highlight the importance of sex as a biological variable in cell culture studies.
The use of nuclear emulsions in very large physics experiments is now possible thanks to the recent improvements in the industrial production of emulsions and to the development of fast automated ...microscopes. In this paper the hardware performances of the
European Scanning System (ESS) are described. The ESS is a very fast automatic system developed for the mass scanning of the emulsions of the OPERA experiment, which requires microscopes with scanning speeds of
∼
20
cm
2
/
h
in an emulsion volume of
44
μ
m
thickness.
The technique of nuclear emulsions for high-energy physics experiments is being revived, thanks to the remarkable progress in measurement automation achieved in the past years. The present paper ...describes the features and performances of the
European Scanning System, a last-generation automatic microscope working at a scanning speed of
20
cm
2
/
h
. The system has been developed in the framework of the OPERA experiment, designed to unambigously detect
ν
μ
→
ν
τ
oscillations in nuclear emulsions.
Precise data on the neutron magnetic form factor Gmn have been obtained with measurements of the ratio of cross sections of D(e,e′n) and D(e,e′p) up to momentum transfers of Q2=0.9 (GeV/c)2. Data ...with typical uncertainties of 1.5% are presented. These data allow for the first time to extract a precise value of the magnetic radius of the neutron.
We have carried out an (e,e'p) experiment at high momentum transfer and in parallel kinematics to measure the strength of the nuclear spectral function S(k,E) at high nucleon momenta k and large ...removal energies E. This strength is related to the presence of short-range and tensor correlations, and was known hitherto only indirectly and with considerable uncertainty from the lack of strength in the independent-particle region. This experiment locates by direct measurement the correlated strength predicted by theory.
A high-precision polarimeter Hauger, M.; Honegger, A.; Jourdan, J. ...
Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment,
04/2001, Letnik:
462, Številka:
3
Journal Article
Recenzirano
Odprti dostop
We have built a polarimeter in order to measure the electron beam polarization in hall C at JLAB. Using a superconducting solenoid to drive the pure-iron target foil into saturation, and a ...symmetrical setup to detect the M
øller electrons in coincidence, we achieve an accuracy of <1%. This sets a new standard for M
øller polarimeters.
Abstract Low aerobic capacity is strongly associated with all-cause mortality and risk for Alzheimer’s disease (AD). Individuals with early dementia and AD have lower aerobic capacity compared to ...age-matched controls. The mechanism by which aerobic capacity influences AD risk is unknown but is likely mediated by sexual dimorphism and tissue-level differences in mitochondrial energetics. Here, we used rats selectively bred for large differences in intrinsic aerobic exercise capacity. Brain tissue from 18-month and 24-month-old female and male low-capacity runner (LCR) and high-capacity runner (HCR) rats were analyzed for markers of mitochondrial function and AD-associated pathologies. LCR rats, irrespective of sex, exhibited a greater increase in brain amyloid beta (Aβ 42 ) and tau hyperphosphorylation (pTau thr181 /total tau) with aging. In female LCR rats, brain mitochondrial respiration at states 3, 4, and FCCP-induced uncoupling, when stimulated with pyruvate/malate, was reduced at 18 and 24 months, leading to lower ATP-linked mitochondrial respiration compared to mitochondria from HCR rats. Male LCR rats also showed reduced complex II-stimulated mitochondrial respiration (succinate + rotenone) at 24 months compared to HCR rats. Differences in mitochondrial respiration were associated with tau hyperphosphorylation and Aβ42 alterations in both HCR and LCR strains. Proteomic analysis unveiled a distinct difference in the mitochondrial proteome, wherein female LCR rats displayed diminished mitochondrial translation and oxidative phosphorylation (OXPHOS) proteins at 18 months compared to female HCR rats. Conversely, male LCR rats exhibited increased OXPHOS protein abundance but reduced tricarboxylic acid (TCA) cycle proteins compared to male HCR rats. These findings underscore a robust association between intrinsic aerobic exercise capacity, brain mitochondrial function, and AD pathologies during aging.
Membrane polarization occurs in sediments with different surface area of capillaries (pores) and is regarded as a slow type of polarization. This phenomenon is the foundation of the well known ...methods of induced polarization (IP): time domain and frequency domain induced polarization. The characteristic parameters of induced polarization which are required for studying physical properties of rocks are measured in the laboratory. Data measured in the laboratory confirmed the distinctions of IP processes at time-on and time-off. Additionally linear dependence of voltage and applied current is not always observed. This paper presents the first step of studying: theoretical consideration for time-on and mathematical modeling of membrane polarization, ion concentrations of electrolyte in the pores of different models of pores space, and arising voltage. The problem of concentration of ions along the pores can be solved using the diffusion equation with specified initial and boundary conditions. Reduced boundary conditions for time-on show that transient concentrations at the boundaries are linear with time. It allows obtaining the analytical solution for this equation. Mathematical modeling has been performed for different combinations of pores. It is shown that if electrical current flows from the pores with greater transfer numbers to the pores with smaller transfer numbers, an excess of ions will be observed at this boundary. If the difference of transfer numbers is negative, there is a decrease in the concentration of ions at the vicinity of the boundary. This decrease will continue until the concentration at this boundary reaches zero. In this case the galvanic chain will be interrupted and electrical current flowing through the sample does not penetrate to this cell. The duration of the process of ions distribution in the pore and time of blockage
t
0
is proportional to the radii of contacted pores and inversely proportional to the transfer number difference and square of the current flowing through this cell. It was shown by both laboratory measurement and field processes that induced polarization relates to low porous rocks with small transfer number differences.
An alternative definition of risk is proposed as risk being a function of the hazard, which is related to the risk source and the vulnerability, which is related to the risk object. The same hazard ...will not cause the same effect on all risk objects. Therefore, vulnerability is introduced as a system-dependent property to be the link between the hazard and the effect so that the combination of the occurrence of a hazard and the vulnerability of an object results in the effect. In risk communication indicators are helpful since they help to simplify the message that has to be communicated. Three examples (pluvial flooding of sewers, dissolved oxygen depletion in streams and discharge of chemicals to receiving waters) show that dependent on the risk problem possibilities for risk reduction lies either at the risk source or at the risk object. Therefore, it is important to have indicators that can be used when the possibilities of risk reduction are analysed.
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