•A series of conductive adhesive self-healing nanocomposite hydrogels were synthesized.•These hydrogels showed remarkable in vivo photothermal antibacterial property.•The hydrogels exhibited good ...hemostatic property and biodegradability.•The hydrogels showed excellent treatment effect for infected wound via photothermal therapy.
Bacteria-infected wounds and antibiotics abuse have become significant burdens to patients and medical systems. Thus, designing a non-antibiotic-dependent multifunctional wound dressing for treating bacteria-infected wounds is urgently desired. Herein, a series of conductive self-healing and adhesive nanocomposite hydrogels with a remarkable photothermal antibacterial property based on N-carboxyethyl chitosan (CEC) and benzaldehyde-terminated Pluronic F127/carbon nanotubes (PF127/CNT) were developed, and their great potential as agents for photothermal therapy (PTT) of infected wounds was demonstrated in vivo. The hydrogels exhibited a suitable gelation time, stable mechanical properties, hemostatic properties, high water absorbency, and good biodegradability. After loading the antibiotic moxifloxacin hydrochloride, the hydrogels showed a pH-responsive release profile and good antibacterial activity. The tissue adhesive property of the hydrogels allowed them to have a good hemostatic effect in a mouse liver trauma model, mouse liver incision model, and mouse tale amputation model. The addition of CNTs endowed the hydrogel with in vitro/in vivo photothermal antimicrobial activity and good conductivity. An in vivo experiment in a mouse full-thickness skin wound-infected model indicated that the hydrogels had an excellent treatment effect leading to significantly enhanced wound closure healing, collagen deposition, and angiogenesis. In summary, these conductive photothermal self-healing nanocomposite hydrogels as multifunctional wound dressing exhibit great potential for the treatment of infected wounds.
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•Nanofibrous wound dressing with comparable mechanical property to soft tissue was designed.•The dressing showed good electroactivity and anti-oxidant ability.•The dressing exhibited ...good biocompatibility and effective antibacterial activity.•The dressing significantly accelerated the healing process of full-thickness skin wound.
Developing a novel nanofibrous wound dressing with multi-functional properties, integrating suitable mechanical property, electroactivity, anti-oxidant and inherent antibacterial activity to promote wound healing process is urgently desired, which could meet the increasing requirements of clinical needs. Herein, a series of antibacterial, anti-oxidant and electroactive nanofibrous membranes were fabricated by electrospinning poly(ε-caprolactone) (PCL) and quaternized chitosan-graft-polyaniline (QCSP) polymer solutions which combined the good mechanical property of PCL and multi-functionality of QCSP. The nanofibrous wound dressings exhibited electroactivity, similar mechanical properties to soft tissue, free radical scavenging capacity, antibacterial property, and biocompatibility. In particular, PCL/QCSP15 (15 wt% of QCSP in the sample) showed a good balanced ability between antibacterial activity and cell proliferation, which significantly accelerated the healing process of wound in a mouse full-thickness wounds defect model than commercial dressing (Tegaderm™ Film) and pure PCL (PCL/QCSP0) nanofibrous membrane. Moreover, the histopathological examination and immunofluorescence staining showed that the wounds treated by PCL/QCSP15 nanofiber dressing exhibited higher collagen deposition, granulation tissue thickness, and more angiogenesis. In one word, these antibacterial, anti-oxidant, electroactivity nanofibrous membranes showed promising applications for full-thickness skin repair.
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•Legacy, short chain and emerging PFASs in residential soils of 89 cities were studied.•Serious pollution of PFASs occurred in Hunan and Eastern Coastal China.•F-53B and short-chain ...analogues already became the dominant PFAS pollutants.•GenX and FC-98 were detectable in residential soil with a relatively low level.
With the worldwide regulation of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS), the alternatives (short chain analogues and emerging per- and polyfluoroalkyl substances, PFASs) have gradually attracted global attention. This study analysed the replacing of legacy PFASs in China using PFASs data from residential soils, which might be good environmental indicators of their present usage. The total concentrations of 21 PFASs ranged from 244 to 13564 pg/g, and PFOA was the dominant compound among the studied PFASs, with a concentration of 354 ± 439 pg/g. Serious PFASs pollution in residential soils mainly occurred in Eastern Coastal China as a result of locally developed industry and economies. Weak but significant correlations were found between PFASs and environmental and socioeconomic factors, suggesting that various factors determine PFASs contamination in residential soils. The concentration and detection frequency (DF) of short-chain analogues (C < 8) (375 ± 509 pg/g and 100%), and F-53B (216 ± 306 pg/g and 98.9%) were higher than those for PFOS (193 ± 502 pg/g and 85.4%), indicating that these compounds have been widely used as PFOS alternatives and their consumption has already exceeded that of PFOS in China. In addition, GenX (the PFOA alternative) had a concentration and DF of 19.1 ± 104 pg/g and 40.5%, respectively. These values were much lower than those for PFOA (354 ± 439 pg/g and 96.6%), indicating GenX consumption is still limited at the national scale of China, despite its use as a PFOA replacement. Moreover, the low concentration and DF of FC-98 (2.31 ± 11.1 pg/g and 27.0%) indicate that its consumption might be negligible. Our study demonstrated that short chain analogues and emerging alternatives have become the dominant PFAS pollutants in Chinese residential soils, and further studies need to be conducted to understand their toxicity and environmental risks.
As the abuse of antibiotics continues to increase, the emergence of antibiotic resistance and unknown drug-resistant bacterial infections pose a great threat on people worldwide. In this work, we ...aimed to develop a series of injectable antimicrobial conductive hydrogels based on glycidyl methacrylate functionalized quaternized chitosan (QCSG), gelatin methacrylate (GM), and graphene oxide (GO) for drug-resistant bacterial disinfection and infectious wound healing. The rheology, morphology, mechanical properties, and electrical and photothermal properties of the hydrogels were characterized. Furthermore, the good in vitro and in vivo intrinsic antibacterial, photothermal antibacterial, and antibiotics released antibacterial properties of this multiantibacterial hydrogel were verified. The good biocompatibility of these hydrogels was also investigated by cytocompatibility, hemocompatibility, and histocompatibility tests. In the drug-resistant Methicillin-resistant Staphylococcus aureus (MRSA) infected mouse full-thickness defect model, the wound closure rate, the length of dermal tissue gap, number of blood vessels and hair follicles in hematoxylin-eosin (HE) staining, the amount of collagen in Masson staining, and the related cytokines for the expression of inflammation (interleukin-6, IL-6) and regeneration of blood vessels (vascular endothelial growth factor, VEGF) in immunofluorescence were all further studied. All the results demonstrated the better wound healing effect of these multiantibacterial injectable conductive hydrogel in infectious skin tissue defect repair, indicating their great potential for infected wound healing.
Small cell lung cancer (SCLC) is a refractory cancer with poor prognosis due to its aggressive malignancy and high rates of metastasis, recurrence and drug resistance. These characteristics have also ...greatly impeded the identification of new treatment methods and drugs. The traditional model of SCLC treatment that has been reliant on platinum combined with etoposide for decades has been superseded by the emergence of immune checkpoint inhibitors (ICIs), which have shown significant therapeutic effects and broad application prospects as a monotherapy. This has led to the evaluation of ICIs with different mechanisms of action and their use in combination with radiotherapy or a variety of molecular targeted drugs to achieve synergy, complementary advantages, and reduce adverse reactions. Here, we review the progress in the use of ICIs as a monotherapy or in combination therapy for SCLC and consider the current limitations of these approaches as well as prospects for future developments.
Prediction of enzyme kinetic parameters is essential for designing and optimizing enzymes for various biotechnological and industrial applications, but the limited performance of current prediction ...tools on diverse tasks hinders their practical applications. Here, we introduce UniKP, a unified framework based on pretrained language models for the prediction of enzyme kinetic parameters, including enzyme turnover number (k
), Michaelis constant (K
), and catalytic efficiency (k
/ K
), from protein sequences and substrate structures. A two-layer framework derived from UniKP (EF-UniKP) has also been proposed to allow robust k
prediction in considering environmental factors, including pH and temperature. In addition, four representative re-weighting methods are systematically explored to successfully reduce the prediction error in high-value prediction tasks. We have demonstrated the application of UniKP and EF-UniKP in several enzyme discovery and directed evolution tasks, leading to the identification of new enzymes and enzyme mutants with higher activity. UniKP is a valuable tool for deciphering the mechanisms of enzyme kinetics and enables novel insights into enzyme engineering and their industrial applications.
With long-term metabolic malfunction, diabetes can cause serious damage to whole-body tissue and organs, resulting in a variety of complications. Therefore, it is particularly important to further ...explore the pathogenesis of diabetes complications and develop drugs for prevention and treatment. In recent years, different from apoptosis and necrosis, ferroptosis has been recognized as a new regulatory mode of cell death and involves the regulation of nuclear receptor coactivator 4 (NCOA4)-mediated ferritinophagy. Evidence shows that ferroptosis and ferritinophagy play a significant role in the occurrence and development of diabetes complications.
we systematically review the current understanding of ferroptosis and ferritinophagy, focusing on their potential mechanisms, connection, and regulation, discuss their involvement in diabetes complications, and consider emerging therapeutic opportunities and the associated challenges with future prospects.
In summary, ferroptosis and ferritinophagy are worthy targets for the treatment of diabetes complications, but their complete molecular mechanism and pathophysiological process still require further study.
Anomaly detection based on telemetry data is a major issue in satellite health monitoring which can identify unusual or unexpected events, helping to avoid serious accidents and ensure the safety and ...reliability of operations. In recent years, sparse representation techniques have received an increasing amount of interest in anomaly detection, although its applications in satellites are still being explored. In this paper, a novel sparse feature-based anomaly detection method (SFAD) is proposed to identify hybrid anomalies in telemetry. First, a telemetry data dictionary and the corresponding sparse matrix are obtained through K-means Singular Value Decomposition (K-SVD) algorithms, then sparse features are defined from the sparse matrix containing the local dynamics and co-occurrence relations in the multivariate telemetry time series. Finally, lower-dimensional sparse features vectors are input to a one-class support vector machine (OCSVM) to detect anomalies in telemetry. Case analysis based on satellite antenna telemetry data shows that the detection precision, F1-score and FPR of the proposed method are improved compared with other existing multivariate anomaly detection methods, illustrating the good effectiveness of this method.
Highlights
A series of novel injectable pH-responsive self-healing hydrogels with enhanced adhesive strength were prepared.
The hydrogels showed good gastric hemostasis property in a swine gastric ...hemorrhage model.
The hydrogels greatly enhanced gastric wound healing in a swine gastric wound model.
Endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) are well-established therapeutics for gastrointestinal neoplasias, but complications after EMR/ESD, including bleeding and perforation, result in additional treatment morbidity and even threaten the lives of patients. Thus, designing biomaterials to treat gastric bleeding and wound healing after endoscopic treatment is highly desired and remains a challenge. Herein, a series of injectable pH-responsive self-healing adhesive hydrogels based on acryloyl-6-aminocaproic acid (AA) and AA-g-N-hydroxysuccinimide (AA-NHS) were developed, and their great potential as endoscopic sprayable bioadhesive materials to efficiently stop hemorrhage and promote the wound healing process was further demonstrated in a swine gastric hemorrhage/wound model. The hydrogels showed a suitable gelation time, an autonomous and efficient self-healing capacity, hemostatic properties, and good biocompatibility. With the introduction of AA-NHS as a micro-cross-linker, the hydrogels exhibited enhanced adhesive strength. A swine gastric hemorrhage in vivo model demonstrated that the hydrogels showed good hemostatic performance by stopping acute arterial bleeding and preventing delayed bleeding. A gastric wound model indicated that the hydrogels showed excellent treatment effects with significantly enhanced wound healing with type I collagen deposition, α-SMA expression, and blood vessel formation. These injectable self-healing adhesive hydrogels exhibited great potential to treat gastric wounds after endoscopic treatment.
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•Contamination of MPs in Bohai Bay coast was at moderate level.•Total number of MPs in the sediments of Bohai Sea was 534 trillion.•Densities of some PE and PP particles were higher ...than that of seawater.•Density increasing rates were 7.4%–12.6% and 12.1%–17.5% for PE and PP, respectively.•Density increasing was caused by the co-contribution of biofouling and minerals.
Size, shape and color are the basic parameters of environmental microplastic (MPs). However, the density of microplastics which is an important parameter to influence the fate of MPs in the environment has not been measured. Meanwhile, the MPs characteristics from coastal sediments of Bohai Bay remain unclear. In this study, the concentrations, distribution and composition (shapes and sizes) of MPs were investigated. The average concentration of MPs in the coastal sediments of Bohai Bay was 192.3 ± 138.9 items/kg dw, which was at a moderate pollution level. The MPs with fiber shape (54.5%), small size (52.0%) and low density (PE and PP) were dominant. In addition, flotation experiment showed that most of PE and PP had the higher density compared with seawater. Digestion experiments suggested that the density change of MPs was caused by the co-contribution of biofouling and inorganic minerals. The density increasing rates of PE and PP were 7.4%–12.6% and 12.1%–17.5%, respectively. Finally, we also estimated that the total number of MPs in the sediments of Bohai Sea was 534 trillion, which were significantly higher than that in global ocean surface, suggesting that the coastal sediments could be the sink of MPs.
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