Tumor necrosis factor receptor‐associated factor‐6 (TRAF6) is a ubiquitin E3 ligase. TRAF6 plays an important role in tumor invasion and metastasis. However, the specific mechanism by which TRAF6 ...promotes colorectal cancer (CRC) metastasis is incompletely understood. This study aimed to determine whether TRAF6 affects the LPS‐NF‐κB‐VEGF‐C signaling pathway through ubiquitination, which plays a role in colorectal cancer metastasis. Here, our results showed that TRAF6 affected lymphangiogenesis through the LPS‐NF‐κB‐VEGF‐C signaling pathway. Using ubiquitination experiments, we found that TRAF6 was mainly ubiquitinated with the K63‐linked chains, and LPS promoted ubiquitination of TRAF6 and K63‐linked chains. More importantly, TRAF6 124mut is the main ubiquitination site of TRAF6 interacting with K63‐linked chains. TRAF6 affected the migration, invasion, and lymphatic metastasis of colorectal cancer through its ubiquitination. In subcutaneous xenograft models, TRAF6 124mut inhibited tumor growth. In conclusion, our results provide new insight for studying the mechanism of lymphangiogenesis in colorectal cancer to promote cancer metastasis, which may provide new ideas for tumor immunotherapy.
TRAF6 regulates the LPS‐NF‐kappaB‐VEGF‐C signal pathway to effect colorectal cancer lymphangiogenesis, growth and invasion via a ubiquitination mechanism. Our results provide new insights into the mechanism of colorectal cancer metastasis, which may provide novel ideas for tumor immunotherapy.
In the field of continuous-variable quantum information processing, non-Gaussian states with negative values of the Wigner function are crucial for the development of a fault-tolerant universal ...quantum computer. While several non-Gaussian states have been generated experimentally, none have been created using ultrashort optical wave packets, which are necessary for high-speed quantum computation, in the telecommunication wavelength band where mature optical communication technology is available. In this paper, we present the generation of non-Gaussian states on wave packets with a short 8-ps duration in the 1545.32 nm telecommunication wavelength band using photon subtraction up to three photons. We used a low-loss, quasi-single spatial mode waveguide optical parametric amplifier, a superconducting transition edge sensor, and a phase-locked pulsed homodyne measurement system to observe negative values of the Wigner function without loss correction up to three-photon subtraction. These results can be extended to the generation of more complicated non-Gaussian states and are a key technology in the pursuit of high-speed optical quantum computation.
Colorectal cancer cannot be completely cured at present, and it is still an important clinical medical problem. TRAF6 is highly expressed in many malignant tumors. However, the role of TRAF6 in ...colorectal cancer is still controversial, mainly because the specific regulatory mechanism of colorectal cancer is still unclear, and the death mode of colorectal cancer cells has not been elucidated. The recent study found that TRAF6 inhibits necroptosis in colorectal cancer cells via the RIPK1/RIPK3/MLKL signaling pathway. The RIPK1 inhibitor Necrostain-1 inhibits colorectal cancer cell necroptosis via the RIPK1/RIPK3/MLKL signaling pathway. TRAF6 directly interacts with RIPK1 through the polyubiquitination of Lys48-linked RIPK1 and reduces the levels of RIPK1 protein in colorectal cancer cells, leading to necroptosis, thus promoting the proliferation of colorectal cancer cells. The recent study demonstrated that TRAF6 promotes colorectal cell progression by inhibiting the RIPK1/RIPK3/MLKL necroptosis signaling pathway, which may provide a new therapeutic target for colorectal cancer.
Market segmentation is common across various sectors, particularly evident in China's electricity market. Meanwhile, officials form a variety of geographic networks due to their innate backgrounds ...and acquired social connections. An extensive body of studies providing insights into the important role of officials' network ties in resource allocation, but little attention has been paid to the impact of this dynamic process on energy resources. Given that energy plays a significant role in shaping economic growth, our study complements this body of literature by examining how leaders with hometown ties impact inter-provincial electricity trade in China using a gravity model. The gravity model, which has become the traditional framework for estimating the determinants of trade flows, is applicable to the province pairs data structure in our study. Using data on provincial officials' hometown and inter-provincial electricity transactions for 30 provinces in China between 2007 and 2019, the results reveal that a leader whose hometown is i and current jurisdiction is j significantly increases the probability of electricity flowing from i to j, corresponding to a 53.73 % increase, which indicates that they implement a weaker market segmentation on their hometown. Moreover, the results reveal that this effect only occurs in a leader's tenure, and is particularly pronounced when a leader's hometown and current jurisdiction are on the same regional grid, or at least adjacent to each other, or when the current jurisdiction has higher power load levels. The results of mechanism analysis reveal that China's immature electricity market drives officials to leverage hometown ties for market integration, rather than promotion or rent-seeking. Our findings contribute to a deeper understanding of the broader impact of officials' personal ties. It also provides a valuable insight into the factors facilitating the integration of China's electricity market.
•A gravity model is used to explore how leaders' hometown ties affect power trade.•Hometown ties increase power trade between a leader's hometown and current jurisdiction.•Hometown ties effects show a temporal, spatial and power load heterogeneity.•An immature power market drives officials to utilize hometown ties for market integration.
Inflammatory bowel disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), is a chronic, inflammatory, and autoimmune disease, but its specific etiology and pathogenesis are still ...unclear. This study aimed to better discover the causative basement membrane (BM) genes of their subtypes and their associations.
The differential expression of BM genes between CD and UC was analyzed and validated by downloading relevant datasets from the GEO database. We divided the samples into 3 groups for comparative analysis. Construction of PPI networks, enrichment of differential gene functions, screening of Lasso regression models, validation of ROC curves, nomogram for disease prediction and other analytical methods were used. The immune cell infiltration was further explored by ssGSEA analysis, the immune correlates of hub BM genes were found, and finally, the hub central genes were screened by machine learning.
We obtained 6 candidate hub BM genes related to cellular immune infiltration in the CD and UC groups, respectively, and further screened the central hub genes ADAMTS17 and ADAMTS9 through machine learning. And in the ROC curve models, AUC > 0.7, indicating that this characteristic gene has a more accurate predictive effect on IBD. We also found that the pathogenicity-related BM genes of the CD and UC groups were mainly concentrated in the ADAMTS family (ADAMTS17 and ADAMTS9). Addition there are some differences between the two subtypes, and the central different hub BM genes are SPARC, POSTN, and ADAMTS2.
In the current study, we provided a nomogram model of CD and UC composed of BM genes, identified central hub genes, and clarified the similarities and differences between CD and UC. This will have potential value for preclinical, clinical, and translational guidance and differential research in IBD.
Colorectal cancer (CRC) is a formidable disease due to the intricate mechanisms that drive its proliferation and metastasis. Despite significant progress in cancer research, the integration of these ...mechanisms that influence cancer cell behavior remains elusive. Therefore, it is imperative to comprehensively elucidate the underlying mechanisms driving CRC proliferation and metastasis. In this study, we reported a novel role of SLC26A3 in suppressing CRC progression. We found that SLC26A3 expression was downregulated in CRC, which was proportionally correlated with survival. Our in vivo and in vitro experiments demonstrated that up-regulation of SLC26A3 inhibited CRC proliferation and metastasis, while down-regulation of SLC26A3 promoted CRC progression by modulating the expression level of IκB. Furthermore, we identified NHERF2 as a novel interacting protein of SLC26A3 responsible for stabilizing the IκB protein and removing ubiquitination modification. Mechanistically, SLC26A3 augmented the interaction between NHERF2 and IκB, subsequently reducing its degradation. This process inhibited the dissociation of p65 from the IκB/p65/p50 complex and reduced the translocation of p65 from the cytoplasm to the nucleus. Moreover, our investigation revealed that NF-κB/p65 directly bound to the promoter of SLC26A3, leading to a decline in its mRNA expression. Thus, SLC26A3 impeded the nuclear translocation of NF-κB/p65, enhancing the transcription of SLC26A3 and establishing a positive regulatory feedback loop in CRC cells. Collectively, these results suggest that a SLC26A3/NHERF2-IκB/NF-κB/p65 signaling loop suppresses proliferation and metastasis in CRC cells. These findings propose a novel SLC26A3-driven signaling loop that regulates proliferation and metastasis in CRC, providing promising therapeutic interventions and prognostic targets for the management of CRC.
Coordinating the energy mix transition with environmental protection is essential for reducing climate risks. Long-term fossil fuel exploitation and utilization result in increasing ecological risks ...in energy-rich regions. While ultra-high voltage (UHV) transmission is considered a key tool for promoting long-distance energy consumption, its ecological impact has received little attention. Using city-level panel data from 2005 to 2019 in China, this study examines the impact of UHV transmission on eco-environmental quality in energy-rich regions. The empirical results show that UHV transmission reduces eco-environmental quality in energy-rich regions. This effect is particularly pronounced in regions with more abundant fossil energy sources, underdeveloped economies, and weak environmental regulations. Mechanism analysis finds that UHV transmission promotes the expansion of power generation in energy-rich regions, followed by lagging industrial structure, weakening eco-environmental governance and intensifying resource exploitation. These findings suggest that increasing the utilization of renewable energy and strengthening environmental regulation are essential for achieving sustainable development in energy-rich regions.
•This study examines the impact of UHV transmission on eco-environmental quality of energy-rich regions.•UHV transmission reduces eco-environmental quality in energy-rich regions.•Mechanisms include lagging industrial mix, weakening eco-environmental governance, and intensifying resource exploitation.
To cope with global climate risks, promoting energy transformation has become a global consensus. China issued a reform policy in 2010 to promote the convergence of the three major information ...industries, namely broadcasting and television networks, telecommunications networks and the Internet (TPR policy). However, is the convergence of information industries able to promote energy transition? This study constructs a quasi-natural experimental framework using China's TPR policy as a representative case of industrial convergence. Using city-level panel data between 2003 and 2016 in China and a multi-period difference-in-differences model, this study examines the impact of TPR policy on energy transition. The results show that TPR policy positively contributes to energy transition at a 1% significance level. Extensive robustness tests support this finding. This positive effect is particularly evident in regions with less developed communications infrastructure and stricter environmental regulations. Mechanism analysis suggests that TPR policy promotes energy transition by improving the level of information networks, promoting technological innovation, and optimizing industrial structure. The findings of this paper emphasize the importance of information industry convergence in facilitating energy transformation.
•This paper examines the impact of TPR on energy transition by using a DID model.•TPR promotes energy transition at a 1% significance level.•TPR's effect shows heterogeneity in the level of communication and regulations.•Information level, innovation and industrial upgrading play a key role in this process.
Administrative officials are often transferred during the course of their careers, yet little attention has been paid to how their transfer might affect energy transactions across regions. By ...employing data on the transfer of provincial leaders between provinces and inter-provincial electricity transactions in China, we examine the impact of local leaders’ transfer on electricity transactions and the underlying mechanisms. The findings show that the transfer of local leaders leads to an increase in inter-regional electricity transactions in the direction of the transfer. This effect disappears when the transferred leaders leave office. The boosts are more accessible when a leader’s previous and current jurisdictions are on the same regional grid, or at least adjacent to each other, or when the current jurisdiction embraces a heavier power load. Based on insights from political economy, these empirical findings can be attributed to the motivation of provincial leaders for career advancement and the absence of a fully competitive electricity market in China. Our study adds empirical evidence in support of the tournament theory of political promotion. It also provides novel insights into how developing countries might achieve a better allocation of their energy resources.
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•The AgNW-10-W cotton fabric were prepared by dip-coating technology.•The AgNW-10-W cotton fabric shows the EMI SE as high as 53.9 dB with thickness of 1.3 mm.•The AgNW-10-W cotton ...fabric can reach 76 °C under 2 V applied voltage in 60 s.•AgNW-10-W cotton fabric exhibits excellent bending and salt water resistance.•The pHRR of AgNW-10-W cotton fabric are reduced by 31.3%.
High-performance modern integrated intelligent wearable electronic textiles show excellent joule heating performance, electromagnetic interference shielding (EMI) and fire safety. In this work, cotton fabric as a biomass material is used as the framework. Due to its strong electrostatic interaction and hydrogen bonding, 3‑aminopropyl trethoxy silane (KH550) layer, silver nanowire (AgNW) conductive network and water-based polyurethane (WPU) protective layer are formed on the surface of cotton fiber for fabricating composite fabric (AgNW-10-W). Due to the perfect collaborative conductive network and porous fiber network structure, AgNW-10-W shows excellent EMI shielding effectiveness (53.9 dB, 1.3 mm thickness). Furthermore, the surface temperature of the joule heating composite fabric with AgNW as the conductive raw material can reach over 100 °C at an applied voltage of 2 V. Meantime, the composite fabric also shows good cycling and long-term joule heating performance. In the wear tests, the AgNW-10-W cotton fabric has good bending resistance and salt water resistance. It produces heat evenly on the human body's wrist joint and has a certain wear function. In the combustion performance test, the pHRR of AgNW-10-W cotton fabric was 31.3 % lower than that of pure cotton fabric. The work demonstrates the appeal of high-performance multifunctional electronic textiles for joule heating, electromagnetic shielding, and fire safety applications in AI and emerging wearable electronics.
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