Arid ecosystems cover ∼40% of the Earth's terrestrial surface and store a high proportion of the global nitrogen (N) pool. They are low-productivity, low-biomass, and polyextreme ecosystems, i.e., ...with (hyper)arid and (hyper)oligotrophic conditions and high surface UV irradiation and evapotranspiration. These polyextreme conditions severely limit the presence of macrofauna and -flora and, particularly, the growth and productivity of plant species. Therefore, it is generally recognized that much of the primary production (including N-input processes) and nutrient biogeochemical cycling (particularly N cycling) in these ecosystems are microbially mediated. Consequently, we present a comprehensive survey of the current state of knowledge of biotic and abiotic N-cycling processes of edaphic (i.e., open soil, biological soil crust, or plant-associated rhizosphere and rhizosheath) and hypo/endolithic refuge niches from drylands in general, including hot, cold, and polar desert ecosystems. We particularly focused on the microbially mediated biological nitrogen fixation, N mineralization, assimilatory and dissimilatory nitrate reduction, and nitrification N-input processes and the denitrification and anaerobic ammonium oxidation (anammox) N-loss processes. We note that the application of modern meta-omics and related methods has generated comprehensive data sets on the abundance, diversity, and ecology of the different N-cycling microbial guilds. However, it is worth mentioning that microbial N-cycling data from important deserts (e.g., Sahara) and quantitative rate data on N transformation processes from various desert niches are lacking or sparse. Filling this knowledge gap is particularly important, as climate change models often lack data on microbial activity and environmental microbial N-cycling communities can be key actors of climate change by producing or consuming nitrous oxide (N
O), a potent greenhouse gas.
Coxiellosis, caused by the bacterium Coxiella burnetii, is a reportable disease in animals and humans in Switzerland. The number of cases in farm animals and humans has risen continuously in recent ...years. The aim of this work was to investigate abortions and stillbirths in goats with a focus on C. burnetii, to identify excretory routes which pose a zoonotic risk and the excretion time after an acute infection. Besides the submitted fetuses, does were screened with a serological antibody test. In addition, excretion via milk, faeces and vaginal mucus were investigated in dams with fetuses tested positive for C. burnetii at 14-day intervals.C. burnetii were isolated in 8 cases (3× in the placenta, 2× in the abomasum, 3× in the placenta and abomasum) of 13 examined stillbirths/abortions. Ten abomasums of goat kids and 8 placentas were examined using modified Ziehl-Neelsen staining (ZN) according to Stamp simultaneously with a real-time PCR. Four of 18 samples were false negative using modified ZN staining according to Stamp in contrast to real-time PCR. Seven does had serum antibodies against Coxiella. The excretion of C. burnetii persisted for 63 days in the milk, for 96 days in the vaginal mucus and for 96 respectively 114 days in two does monitored extensively. Intermittent excretion could also be observed in the milk during these 63 days. The present study showed that confirmation of disease, respectively transmission cannot be based on a single test. Only combined serological antibody test and real-time PCR examinations of birth material, milk, feces and vaginal mucus can result in a conclusive diagnosis. In addition, the examination using modified ZN staining according to Stamp is less sensitive and specific than the real-time PCR examination.
Ancient Ostia at the mouth of the River Tiber into the Tyrrhenian Sea was largely significant for the economic supply of Rome. Ostia itself experienced an extraordinary period of prosperity in the ...second century AD. Starting in AD 42, a first new harbour at Portus was built by Emperor Claudius close to Ostia. It reached its full functionality under Emperor Trajan in the early second century AD, only. At Ostia itself, previous archaeological and geoarchaeological studies have brought to light a lagoon-type harbour at the western fringe of the city operating between the fourth and the second century BC in an artificially excavated harbour basin. From the second century BC onwards, a considerably smaller and shallower part of this western harbour basin was still in function as a fluvial harbour. So far, it was unclear whether Ostia’s western harbour was still in use when the harbour at Portus was set into function in the first to second century AD, or if the latter partially replaced Ostia’s harbour infrastructure. According to archaeological evidence, Ostia’s navalia-temple-complex, the main building at the eastern fringe of the western river harbour basin, was built in the second quarter of the first century AD. Was this prestigious harbour building erected although the associated harbour seemed to have been already given up before? We conducted detailed geoarchaeological investigations at the immediate western front of the navalia-temple complex. Results were compared with archaeological data obtained from excavations carried out in 2000/2001. A multi-proxy approach was used to reconstruct the history and evolution of the harbour. It was possible to identify subsurface structures and evaluate the local stratigraphy. Vibracoring brought to light a more than 1 m thick section of an
opus reticulatum
wall with parts of the original
opus latericium
on top. Such walls originally separated vaulted shipshed chambers of the navalia-temple complex at Ostia, which in turn formed the substructure of a temple complex located above it. Another core revealed the sedimentary infill of a former chamber of the building. Based on radiocarbon dating, the navalia was in use between the first and the fourth centuries AD with a water depth of maximum ca. 1.2 m at the immediate western front. This is in agreement with the date of construction of the navalia-temple complex in the second quarter of the first century AD. The relative sea level at that time was around 0.64 m below the present sea level. The harbour and the navalia were obviously accessible only for flat-keeled lighters and cargo boats. Larger cargo ships were either unloaded along the riverbank to the north of ancient Ostia (Hadler et al.
2019
) or moored offshore, their freight being reloaded to smaller lighters. Chronostratigraphic data further show that the navalia-temple complex was in use until the second half of the fourth century AD. It was not before AD 355–363 or shortly afterwards, that the harbour site was abandoned. Ostia’s western river harbour was neither abandoned nor completely silted up before the harbour at Portus was established as previously assumed by other authors. Actually, the western front of the navalia-temple complex was hit by an extreme wave event, leaving a sand layer approx. 0.5 m thick, at or shortly after AD 355–363 which led to the final abandonment of Ostia’s western river harbour. This event is interpreted as a tsunami that may have hit the wider coastal region.
Thermal barrier coatings (TBC) are applied on cooled gas turbine components. Between the heated and cooled surfaces a thermal gradient develops, resulting for constraint components in high multiaxial ...stresses, which may exceed stresses due to mechanical loading. In order to investigate the damage behaviour of TBC systems under close to reality conditions, thermal mechanical fatigue tests with controlled thermal gradients (TGMF-tests) were performed on coated tubular specimens. The specimen substrate was made from directionally solidified nickel base superalloy IN100 DS, and the coating system comprised a NiCoCrAlY bond coat and a ceramic top coat from partially stabilized zirconia. The stress distribution over the specimen wall, which was generated in the course of one TGMF cycle, has been analysed by linear elastic Finite Element calculations.
In TGMF testing specific damages occurred underneath the adherent ceramic top coat, evolving into fatigue cracks, which propagated primarily in the metallic bond coat parallel to the surface. In length sections the crack shape in this stage resembles a ‘smiley’. During further cycling the crack path deviated to the interface between BC and top coat, enhancing spallation. The ‘smiley’-crack patterns, which only occurred in TGMF but not in isothermal low cycle fatigue or thermal fatigue, are discussed with respect to the cyclic local stress distribution.
TBI-based preparative regimens are considered as standard conditioning therapy for allogeneic stem cell transplantation (AHSC) in patients with ALL. We investigated toxicity and efficacy of a ...non-TBI-based regimen consisting of treosulfan, etoposide and cyclophosphamide for ALL within a prospective study. Major inclusion criteria were CR and non-eligibility for TBI. Fifty patients with a median age of 46.5 years (range, 18-64) were included. Donors were HLA-identical sibling (n=8), matched (n=42) or mismatched (n=10) unrelated. The toxicity was moderate, resulting in a cumulative incidence of non-relapse mortality (NRM) at 1 year of 8% (90% confidence interval: 2-15%). Acute GvHD grade II-IV and grade III/IV was noted in 53% and 14%, respectively. Chronic GvHD at one year was seen in 41%. After a median follow-up of 24 months the cumulative incidence of relapse was 36% (90% confidence interval: 24-48) and 51% (90% confidence interval: 37-65) at 1 and 2 years, respectively. The estimated 2-year disease-free and overall survivals were 36 and 48%, respectively. Treosulfan, etoposide and cyclophosphamide followed by AHSC has a favorable toxicity profile with low NRM and therefore represents a potential alternative regimen for ALL in 1. CR (NCT00682305).
Ruptures of the patellar and/or quadriceps tendon are rare injuries that require immediate repair to re-establish knee extensor continuity and to allow early motion. We evaluated 36 consecutive ...patients with quadriceps or patellar tendon rupture between 1993 and 2000. There were 37 primary ruptures, 3 reruptures, 21 quadriceps and 19 patellar tendon ruptures. Follow up examination (>24 months postoperatively) included the patient's history, assessment of risk factors, clinical examination of both knees, isometric muscle strength measurements and three specific knee scores, Hospital for Special Surgery Score, Knee Society Score and Turba Score, and a short form SF-36. We evaluated 29 patients (26 men) with 33 ruptures (16 patellar tendon, 17 quadriceps tendon). Seven patients were lost to follow up. We found no difference between the range of motion and muscle strength when the injured leg was compared to the non-injured leg.
Risk factors did not influence the four scores, patient satisfaction, pain, muscle strength or range of motion. Multiple injured patients had a significant reduction in muscle strength and circumference, however patient satisfaction did not differ to the non-multiple injured patient group.
Neutrophils and renal failure Heinzelmann, Michael; Mercer-Jones, Mark A.; Passmore, John C.
American journal of kidney diseases,
08/1999, Letnik:
34, Številka:
2
Journal Article
Recenzirano
In many diseases and acute inflammatory disorders, important components of pathological processes are linked to the neutrophils' ability to release a complex assortment of agents that can destroy ...normal cells and dissolve connective tissue. This review summarizes the mechanisms of tissue destruction by neutrophils and the role of kidney-specific factors that promote this effect. Nicotinamide adenine dinucleotide phosphate H (NADPH) oxidase is a membrane-associated enzyme that generates a family of reactive oxygen intermediates (ROI). There is increasing evidence that ROIs are implicated in glomerular pathophysiology: ROIs contribute to the development of proteinuria, alter glomerular filtration rate, and induce morphological changes in glomerular cells. Specific neutrophil granules contain microbicidal peptides, proteins, and proteolytic enzymes, which mediate the dissolution of extracellular matrix, harm cell structures or cell function, and induce acute and potentially irreparable damage. Although both ROI and neutrophil-derived proteases alone have the potential for tissue destruction, it is their synergism that circumvents the intrinsic barriers designed to protect the host. Even small amounts of ROI can generate hypochlorus acid (HOCl) in the presence of neutrophil-derived myeloperoxidase (MPO) and initiate the deactivation of antiproteases and activation of latent proteases, which lead to tissue damage if not properly controlled. In addition, neutrophil-derived phospholipase products such as leukotrienes and platelet-activating factor contribute to vascular changes in acute inflammation and amplify tissue damage. Increasing evidence suggests that mesangial cells and neutrophils release chemotactic substances (eg, interleukin 8), which further promote neutrophil migration to the kidney, activate neutrophils, and increase glomerular injury. Also, the expression of adhesion molecules (eg, intercellular adhesion molecule 1 on kidney-specific cells and beta-2-integrins on leukocytes) has been correlated with the degree of injury in various forms of glomerulonephritis or after ischemia and reperfusion. Together, these results suggest that neutrophils and adhesion molecules play an important role in mediating tissue injury with subsequent renal failure. Conversely, chronic renal failure reduces neutrophil function and thereby can increase susceptibility to infection and sepsis.
Microorganisms are involved in all elemental cycles and therefore it is important to study their metabolism in the natural environment. A recent technique to investigate this is the hydrogen isotopic ...composition of microbial fatty acids, i.e., heterotrophic microorganisms produce fatty acids enriched in deuterium (D) while photoautotrophic and chemoautotrophic microorganisms produce fatty acids depleted in D compared to the water in the culture medium (growth water). However, the impact of factors other than metabolism have not been investigated. Here, we evaluate the impact of growth phase compared to metabolism on the hydrogen isotopic composition of fatty acids of different environmentally relevant microorganisms with heterotrophic, photoautotrophic and chemoautotrophic metabolisms. Fatty acids produced by heterotrophs are enriched in D compared to growth water with εlipid/water between 82 and 359‰ when grown on glucose or acetate, respectively. Photoautotrophs (εlipid/water between -149 and -264‰) and chemoautotrophs (εlipid/water between -217 and -275‰) produce fatty acids depleted in D. Fatty acids become, in general, enriched by between 4 and 46‰ with growth phase which is minor compared to the influence of metabolisms. Therefore, the D/H ratio of fatty acids is a promising tool to investigate community metabolisms in nature.
Methane oxidizing prokaryotes are ubiquitous in oxic and anoxic habitats wherever C1-compounds are present. Thus, methane saturated mud volcano fluids should be a preferred habitat of methane ...consuming prokaryotes, using the readily available electron donors. In order to understand the relevance of methane as a carbon and energy source in mud volcano communities, we investigate the diversity of prokaryotic organisms involved in oxidation of methane in fluid samples from the Salse di Nirano mud volcano field situated in the Northern Apennines. Cell counts were at approximately 0.7×106microbialcells/ml. A fraction of the microbial biomass was identified as ANME (anaerobic methanotroph) archaea by fluorescence in situ hybridization (FISH) analysis. They are associated in densely colonized flakes, of some tens of μm in diameter, embedded in a hyaline matrix. Diversity analysis based on the 16S rDNA genes, retrieved from amplified and cloned environmental DNA, revealed a high proportion of archaea, involved in anaerobic oxidation of methane (AOM). Aerobic methane-oxidizing proteobacteria could be highly enriched from mud volcano fluids, indicating the presence of aerobic methanotrophic bacteria, which may contribute to methane oxidation, whenever oxygen is readily available. The results imply that biofilms, dominated by ANME archaea, colonize parts of the mud volcano venting system.
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