Thermally induced errors account for the majority of fabrication accuracy loss in an uncompensated machine tool. This issue is particularly relevant in the micro-machining arena due to the comparable ...size of thermal errors and the characteristic dimensions of the parts under fabrication. A spindle of a micro-milling machine tool is one of the main sources of thermal errors. Other sources of thermal errors include drive elements like linear motors and bearings, the machining process itself and external thermal influences such as variation in ambient temperature. The basic strategy for alleviating the magnitude of these thermal errors can be achieved by thermal desensitization, control and compensation within the machine tool.
This paper describes a spindle growth compensation scheme that aims towards reducing its thermally-induced machining errors. The implementation of this scheme is simple in nature and it can be easily and quickly executed in an industrial environment with minimal investment of manpower and component modifications.
Initially a finite element analysis (FEA) is conducted on the spindle assembly. This FEA correlates the temperature rise, due to heating from the spindle bearings and the motor, to the resulting structural deformation. Additionally, the structural deformation of the spindle along with temperature change at its various critical points is experimentally obtained by a system of thermocouples and capacitance gages.
The experimental values of the temperature changes and the structural deformation of the spindle qualitatively agree well with the results obtained by FEA. Consequently, a thermal displacement model of the high-speed micro-milling spindle is formulated from the previously obtained experimental results that effectively predict the spindle displacement under varying spindle speeds. The implementation of this model in the machine tool under investigation is expected to reduce its thermally induced spindle displacement by 80%, from 6
microns to less than 1 micron in a randomly generated test with varying spindle speeds.
Soil and water bioengineering (SWBE) is a nature-based solution (NBS) that can be used to stabilize riverbanks with living vegetation. Aside to protecting property and people, SWBE provides benefits ...for human well-being and biodiversity. Its use remains modest in cities, where the presumed benefits are important in a context of biodiversity crisis and warming. Negative public perceptions have been identified as one barrier to the dissemination of NBS. This article studies how environmental expertise influences perceptions and values associated with SWBE, and how the possible differences in perceptions and values induced by knowledge contribute to hindering or promoting the dissemination of these solutions. We carried out an original interdisciplinary study based on a sociological survey and ecological field measurements to characterize: (1) the perceived value that actors associate with several riverbanks equipped with different protection structures (green, hybrid, or gray) according to their level of expertise in the aquatic environment; (2) the interactions between these perceived values and the ecological values measured by restoration ecologists; and (3) the perceived benefits and drawbacks of SBWE techniques. Our results show that the ecological and social benefits provided by NBS are recognized by all, whatever their level of knowledge. Despite this consensus, we observed different hierarchies of value associated with bank protection structures among the surveyed actors, depending on their level of environmental expertise (some prioritising ecological values, others relational values), and these could hinder the dissemination of NBS. The most tangible obstacle to the dissemination of NBS in urban areas relates to the risk perceptions of lay people, who experience a higher sense of vulnerability than they do with traditional gray solutions.
•Respondents value the social and ecological benefits of SWBE whatever their expertise.•Social and ecological benefits of SWBE are a major lever for their dissemination.•Certain value conflicts induced by expertise require SWBE techniques to be adapted.•People with little environmental expertise see SWBE as less efficient against risks.•Proof of effectiveness must be provided with demonstration sites and feedbacks.
We have carried out an (e,e'p) experiment at high momentum transfer and in parallel kinematics to measure the strength of the nuclear spectral function S(k,E) at high nucleon momenta k and large ...removal energies E. This strength is related to the presence of short-range and tensor correlations, and was known hitherto only indirectly and with considerable uncertainty from the lack of strength in the independent-particle region. This experiment locates by direct measurement the correlated strength predicted by theory.
Stimulation of growth factor receptors with tyrosine kinase activity is followed by rapid receptor dimerization, tyrosine autophosphorylation and phosphorylation of signalling molecules such as ...phospholipase C gamma (PLC gamma) and the ras GTPase-activating protein. PLC gamma and GTPase-activating protein bind to specific tyrosine-phosphorylated regions in growth factor receptors through their src-homologous SH2 domains. Growth factor-induced tyrosine phosphorylation of PLC gamma is essential for stimulation of phosphatidylinositol hydrolysis in vitro and in vivo. We have shown that a short phosphorylated peptide containing tyrosine at position 766 from a conserved region of the fibroblast growth factor (FGF) receptor is a binding site for the SH2 domain of PLC gamma (ref. 8). Here we show that an FGF receptor point mutant in which Tyr 766 is replaced by a phenylalanine residue (Y766F) is unable to associate with and tyrosine-phosphorylate PLC gamma or to stimulate hydrolysis of phosphatidylinositol. Nevertheless, the Y766F FGF receptor mutant can be autophosphorylated, and can phosphorylate several cellular proteins and stimulate DNA synthesis. Our data show that phosphorylation of the conserved Tyr 766 of the FGF receptor is essential for phosphorylation of PLC gamma and for hydrolysis of phosphatidylinositol, but that elimination of this hydrolysis does not affect FGF-induced mitogenesis.
By constructing Fv and single-chain Fv (scFv) fragments of antibodies, the variable domains are taken out of their natural context in the Fab fragment, where they are associated with the constant ...domains of the light (CL) and heavy chain (CH1). As a consequence, all residues of the former variable/constant domain interface become solvent exposed. In an analysis of 30 non-redundant Fab structures it was found that at the former variable/constant domain interface of the Fv fragment the frequency of exposed hydrophobic residues is much higher than in the rest of the Fv fragment surface. We investigated the importance of these residues for different properties such as folding in vivo and in vitro, thermodynamic stability, solubility of the native protein and antigen affinity. The experimental model system was the scFv fragment of the anti-fluorescein antibody 4-4-20, of which only 2% is native when expressed in the periplasm of Escherichia coli. To improve its in vivo folding, a mutagenesis study of three newly exposed interfacial residues in various combinations was carried out. The replacement of one of the residues (V84D in VH) led to a 25-fold increase of the functional periplasmic expression yield of the scFv fragment of the antibody 4-4-20. With the purified scFv fragment it was shown that the thermodynamic stability and the antigen binding constant are not influenced by these mutations, but the rate of the thermally induced aggregation reaction is decreased. Only a minor effect on the solubility of the native protein was observed, demonstrating that the mutations prevent aggregation during folding and not of the native protein. Since the construction of all scFv fragments leads to the exposure of these residues at the former variable/constant domain interface, this strategy should be generally applicable for improving the in vivo folding of scFv fragments and, by analogy, also the in vivo folding of other engineered protein domains.
Abstract
Observations and analyses of two tidally recurring, oblique, internal hydraulic jumps at a stratified estuary mouth (Columbia River, Oregon/Washington) are presented. These hydraulic ...features have not previously been studied due to the challenges of both horizontally resolving the sharp gradients and temporally resolving their evolution in numerical models and traditional observation platforms. The jumps, both of which recurred during ebb, formed adjacent to two engineered lateral channel constrictions and were identified in marine radar image time series. Jump occurrence was corroborated by (i) a collocated sharp gradient in the surface currents measured via airborne along-track interferometric synthetic aperture radar and (ii) the transition from supercritical to subcritical flow in the cross-jump direction via shipborne velocity and density measurements. Using a two-layer approximation, observed jump angles at both lateral constrictions are shown to lie within the theoretical bounds given by the critical internal long-wave (Froude) angle and the arrested maximum-amplitude internal bore angle, respectively. Also, intratidal and intertidal variability of the jump angles are shown to be consistent with that expected from the two-layer model, applied to varying stratification and current speed over a range of tidal and river discharge conditions. Intratidal variability of the upchannel jump angle is similar under all observed conditions, whereas the downchannel jump angle shows an additional association with stratification and ebb velocity during the low discharge periods. The observations additionally indicate that the upchannel jump achieves a stable position that is collocated with a similarly oblique bathymetric slope.
Precise data on the neutron magnetic form factor Gmn have been obtained with measurements of the ratio of cross sections of D(e,e′n) and D(e,e′p) up to momentum transfers of Q2=0.9 (GeV/c)2. Data ...with typical uncertainties of 1.5% are presented. These data allow for the first time to extract a precise value of the magnetic radius of the neutron.
Bathymetry is a major factor in determining nearshore and surf zone wave transformation and currents, yet is often poorly known. This can lead to inaccuracy in numerical model predictions. Here ...bathymetry is estimated as an uncertain parameter in a data assimilation system, using the ensemble Kalman filter (EnKF). The system is tested by assimilating several remote sensing data products, which were collected in September 2010 as part of a field experiment at the U.S. Army Corps of Engineers Field Research Facility (FRF) in Duck, NC. The results show that by assimilating remote sensing data alone, nearshore bathymetry can be estimated with good accuracy, and nearshore forecasts (e.g., the prediction of a rip current) can be improved. This suggests an application where a nearshore forecasting model could be implemented using only remote sensing data, without the explicit need for in situ data collection.
Key Points
Field application of a nearshore EnKF data assimilation system
Remote sensing data are assimilated to improve model bathymetry
After assimilating data, model prediction of a rip current is also improved
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