With the aim of gathering temporal trends on bacterial epidemiology and resistance from multiple laboratories in China, the CHINET surveillance system was organized in 2005. Antimicrobial ...susceptibility testing was carried out according to a unified protocol using the Kirby-Bauer method or automated systems. Results were analyzed according to Clinical and Laboratory Standards Institute (CLSI) 2014 definitions. Between 2005 and 2014, the number of bacterial isolates ranged between 22 774 and 84 572 annually. Rates of extended-spectrum β-lactamase production among Escherichia coli isolates were stable, between 51.7 and 55.8%. Resistance of E. coli and Klebsiella pneumoniae to amikacin, ciprofloxacin, piperacillin/tazobactam and cefoperazone/sulbactam decreased with time. Carbapenem resistance among K. pneumoniae isolates increased from 2.4 to 13.4%. Resistance of Pseudomonas aeruginosa strains against all of antimicrobial agents tested including imipenem and meropenem decreased with time. On the contrary, resistance of Acinetobacter baumannii strains to carbapenems increased from 31 to 66.7%. A marked decrease of methicillin resistance from 69% in 2005 to 44.6% in 2014 was observed for Staphylococcus aureus. Carbapenem resistance rates in K. pneumoniae and A. baumannii in China are high. Our results indicate the importance of bacterial surveillance studies.
Mutations in the kinase domain of epidermal growth factor receptor (EGFR) are associated with clinical responsiveness to gefitinib in patients with non-small-cell lung cancers (NSCLC). Recently, we ...have identified many novel EGFR mutations in NSCLC tissues. In this study, we found that gefitinib could suppress the tyrosine phosphorylation of most EGFR mutants better than the wild-type receptor. However, gefitinib had quite variable growth-suppressive effects on different EGFR mutant-expressing cells. All tested EGFR mutants have high basal phosphorylation at multiple tyrosine residues. Upon EGF stimulation, the mutated EGFRs did not have apparently stronger phosphorylation at tyrosines 845, 992, 1,068, and 1,173 than the wild-type receptor. However, stronger phosphorylation at tyrosine 1,045 was observed in the S768I, L861Q, E709G, and G719S mutants. The E746-A750 deletion mutant was less responsive to EGF than the wild-type and other mutant receptors. The S768I, L861Q, E709G, and G719S mutants were refractory to EGF-induced ubiquitination and had more sustained tyrosine phosphorylation. E709G and G719S also lacked EGF-induced receptor downregulation. Our results indicate that, in addition to sensitivity to gefitinib, EGFR mutations also caused various changes in EGFR's regulatory mechanisms, which may contribute to the constitutive activation of EGFR mutants and oncogenesis in NSCLC.
•Collective vehicle parking pattern reveals public charging demand.•Siting charging stations based on travel patterns can improve electrification rate.•Environmental impacts are assessed for ...different charging infrastructure siting scenarios.•Peak vehicle charging demand overlaps with Beijing’s peak load.
Charging infrastructure is critical to the development of electric vehicle (EV) system. While many countries have implemented great policy efforts to promote EVs, how to build charging infrastructure to maximize overall travel electrification given how people travel has not been well studied. Mismatch of demand and infrastructure can lead to under-utilized charging stations, wasting public resources. Estimating charging demand has been challenging due to lack of realistic vehicle travel data. Public charging is different from refueling from two aspects: required time and home-charging possibility. As a result, traditional approaches for refueling demand estimation (e.g. traffic flow and vehicle ownership density) do not necessarily represent public charging demand. This research uses large-scale trajectory data of 11,880 taxis in Beijing as a case study to evaluate how travel patterns mined from big-data can inform public charging infrastructure development. Although this study assumes charging stations to be dedicated to a fleet of PHEV taxis which may not fully represent the real-world situation, the methodological framework can be used to analyze private vehicle trajectory data as well to improve our understanding of charging demand for electrified private fleet. Our results show that (1) collective vehicle parking “hotspots” are good indicators for charging demand; (2) charging stations sited using travel patterns can improve electrification rate and reduce gasoline consumption; (3) with current grid mix, emissions of CO2, PM, SO2, and NOx will increase with taxi electrification; and (4) power demand for public taxi charging has peak load around noon, overlapping with Beijing’s summer peak power.
One hundred and thirty-six
bla
OXA-51
-negative strains were identified from 1,067
Acinetobacter calcoaceticus
–
A. baumannii
complex (ACB complex) isolates, which were collected during October 2010 ...to March 2013 from 15 general hospitals in 10 cities throughout Zhejiang Province, China. Seven of the 136
bla
OXA-51
-negative ACB complex isolates were New Delhi metallo-β-lactamase-1 (NDM-1)-positive, among which three were identified as
A. nosocomialis
and four were identified as
A. pittii
strains using 16S–23S rRNA gene intergenic spacer (ITS) sequencing and partial RNA polymerase β-subunit (
rpoB
) sequencing. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) analysis showed that the seven NDM-positive isolates belonged to three clonal strains with three novel sequence types (STs). Polymerase chain reaction (PCR) assays and DNA sequence analysis of the carbapenemase and other β-lactamase genes indicated that all the isolates harbored the
bla
NDM-1
gene, and that only one strain of
A. nosocomialis
isolates harbored both
bla
NDM-1
and
bla
OXA-23
. All of them were positive for
bla
ADC
, from which three novel
bla
ADC
genes (designated as
bla
ADC-69
,
bla
ADC-70
, and
bla
ADC-71
) were detected for the first time. The presence of
IS
Aba125 upstream of
bla
NDM-1
was identified through genetic environment analysis. Carbapenem resistance can be transferred from
A. nosocomialis
and
A. pittii
to
Escherichia coli
EC600 by the conjugation experiment. Plasmid analysis, DNA hybridization, and extraction experiments indicated that
bla
NDM-1
was located on a plasmid of approximately 50 kb. In conclusion, we characterized the dissemination of NDM-1-positive
A. pittii
strains in Zhejiang Province, China, and reported the NDM-producing
A. nosocomialis
for the first time.
Sweet corn is one of the most important vegetables in the United States and Canada. Here, we present a de novo assembly of a sweet corn inbred line Ia453 with the mutated shrunken2-reference allele ...(Ia453-sh2). This mutation accumulates more sugar and is present in most commercial hybrids developed for the processing and fresh markets. The ten pseudochromosomes cover 92% of the total assembly and 99% of the estimated genome size, with a scaffold N50 of 222.2 Mb. This reference genome completely assembles the large structural variation that created the mutant sh2-R allele. Furthermore, comparative genomics analysis with six field corn genomes highlights differences in single-nucleotide polymorphisms, structural variations, and transposon composition. Phylogenetic analysis of 5,381 diverse maize and teosinte accessions reveals genetic relationships between sweet corn and other types of maize. Our results show evidence for a common origin in northern Mexico for modern sweet corn in the U.S. Finally, population genomic analysis identifies regions of the genome under selection and candidate genes associated with sweet corn traits, such as early flowering, endosperm composition, plant and tassel architecture, and kernel row number. Our study provides a high-quality reference-genome sequence to facilitate comparative genomics, functional studies, and genomic-assisted breeding for sweet corn.
Summary
This prospective randomized clinical study is aimed to evidence the reproductive impairment of frequent scrotal heat exposure. A total of 20 normozoospermic subjects were randomly divided ...into two groups to undergo testicular warming in a 43 °C water bath 10 times, for 30 min each time; the subjects in group 1 underwent testicular warming for 10 consecutive days and those in group 2 once every 3 days. Sperm chromatin structure assay (SCSA), sperm mitochondrial membrane potential (MMP), apoptosis, and seminal plasma‐soluble Fas (sFas) were analyzed before treatment and every 2 weeks after, for a total of 10 times. In group 1, some critical proteins involved in heat stress, hypoxia, structure, and function of sperm mitochondria and flagella were evaluated before hyperthermia and 2, 6, 10, and 16 weeks after hyperthermia. Both groups showed a reversible increase in the proportion of spermatozoa with a disrupted MMP (both p < 0.05 when the minimums were compared with baseline levels, the same below), sperm apoptosis (both p < 0.01) and high DNA stainability (both p < 0.05). The sFas concentration in both groups showed no obvious changes except one: the value at week 2 was significantly increased over baseline in group 1 (p = 0.036). The level of Bcl‐2 decreased significantly at weeks 6 and 10 (p = 0.017 and 0.05, respectively) and recovered to baseline at week 16. Proteins involved in heat stress and mitochondria functions were up‐regulated, whereas in flagella structure and function was down‐regulated (all p < 0.05). This study demonstrated that transient and frequent scrotal hyperthermia severely and reversibly damaged spermatogenesis, consecutive heat exposure had more serious effects than intermittent exposure, whereas intermittent exposure led to a later recovery of sperm damage.
Type 2 diabetes is associated with an increased risk of dementia. This study investigated the global connectivity patterns in the brains of patients with type 2 diabetes by using a functional MR ...imaging technique.
Forty patients and 43 age-, sex-, and education-matched healthy controls underwent resting-state functional imaging in a 3T MR imaging unit. Degree centrality, a commonly used measurement of global connectivity, was computed for a full-brain exploration of the regions influenced by type 2 diabetes. We then examined the functional connectivity of each region by using the seed-based approach. Finally, voxelwise correlation analyses were performed to explore the relationship among the connectivity changes, cognitive performance, and diabetes-related variables.
Patients exhibited decreased degree centrality in the left lingual gyrus and increased centrality in the right insula and dorsal anterior cingulate cortex (corrected
< .05). The occipital network anchored in the lingual gyrus showed extensively reduced connectivity, while the network connectivity of the insula and cingulate cortex (mostly included in the salience network) was significantly elevated (corrected
< .05). Correlational analyses revealed that in the diabetic group, impaired visual memory and executive function performance were correlated with occipital hypoconnectivity, while higher fasting plasma glucose levels and better executive functioning were related to anterior cingulate cortex hyperconnectivity (all corrected
values < .05). Similar effects were not detected in the controls.
This preliminary study shows that network connectivity is altered in patients with type 2 diabetes, which may provide critical insight into the neural substrate of diabetes-related cognitive decline.
A new class of ignition designs is proposed for inertial confinement fusion experiments. These designs are based on the hot-spot ignition approach, but instead of a conventional target that is ...comprised of a spherical shell with a thin frozen deuterium-tritium (DT) layer, a liquid DT sphere inside a wetted-foam shell is used, and the lower-density central region and higher-density shell are created dynamically by appropriately shaping the laser pulse. These offer several advantages, including simplicity in target production (suitable for mass production for inertial fusion energy), absence of the fill tube (leading to a more-symmetric implosion), and lower sensitivity to both laser imprint and physics uncertainty in shock interaction with the ice-vapor interface. The design evolution starts by launching an ∼ 1 -Mbar shock into a DT sphere. After bouncing from the center, the reflected shock reaches the outer surface of the sphere and the shocked material starts to expand outward. Supporting ablation pressure ultimately stops such expansion and subsequently launches a shock toward the target center, compressing the ablator and fuel, and forming a shell. The shell is then accelerated and fuel is compressed by appropriately shaping the drive laser pulse, forming a hot spot using the conventional or shock ignition approaches. This Letter demonstrates the feasibility of the new concept using hydrodynamic simulations and discusses the advantages and disadvantages of the concept compared with more-traditional inertial confinement fusion designs.
We report results on the searches of weakly interacting massive particles (WIMPs) with sub-GeV masses (mχ) via WIMP-nucleus spin-independent scattering with Migdal effect incorporated. Analysis on ...time-integrated (TI) and annual modulation (AM) effects on CDEX-1B data are performed, with 737.1 kg day exposure and 160 eVee threshold for TI analysis, and 1107.5 kg day exposure and 250 eVee threshold for AM analysis. The sensitive windows in mχ are expanded by an order of magnitude to lower DM masses with Migdal effect incorporated. New limits on σχNSI at 90% confidence level are derived as 2×10−32∼7×10−35 cm2 for TI analysis at mχ∼50–180 MeV/c2, and 3×10−32∼9×10−38 cm2 for AM analysis at mχ∼75 MeV/c2–3.0 GeV/c2.
Plants evolve stress-specific responses that sense changes in their external environmental conditions and develop various mechanisms for acclimatization and survival. Calcium (Ca
) is an essential ...stress-sensing secondary messenger in plants. Ca
sensors, including calcium-dependent protein kinases (CDPKs), calmodulins (CaMs), CaM-like proteins (CMLs), and calcineurin B-like proteins (CBLs), are involved in jasmonates (JAs) signalling and biosynthesis. Moreover, JAs are phospholipid-derived phytohormones that control plant response to abiotic stresses. The JAs signalling pathway affects hormone-receptor gene transcription by binding to the basic helix-loop-helix (bHLH) transcription factor. MYC2 acts as a master regulator of JAs signalling module assimilated through various genes. The Ca
sensor CML regulates MYC2 and is involved in a distinct mechanism mediating JAs signalling during abiotic stresses. This review highlights the pivotal role of the Ca
sensors in JAs biosynthesis and MYC2-mediated JAs signalling during abiotic stresses in plants.
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