Detection of plant viruses by ELISA using different reagent strips Reichenbaecher, D. (Akademie der Landwirtschaftswissenschaften der Deutschen Demokratischen Republik, Aschersleben (German D.R.). Inst. fuer Phytopathologie); Reiss, E; Hunger, H.-D ...
Journal of phytopathology,
04/1990, Letnik:
128, Številka:
4
Journal Article
Recenzirano
A simplified immunoassay for detection of plant viruses under field conditions was developed on the basis of direct double antibody sandwich ELISA using dry reagent carriers immobilized on ...PVC-supports which are arranged in a fan-like manner. The fan consists of a first reagent carrier with antivirus IgG covalently bound to cyanuric chloride-activated (CCA) paper while the second and third are filter papers impregnated with alkaline phophatase labelled antivirus IgG and the fluorogenic substrate 4-methylumbelliferylphosphate, respectively. Performing the test, the first carrier is contacted with a liquid sample containing the virus to be identified, the second and third carriers are sequentially put one upon the other and the reactions carried out. The virus is detected by the reacted substrate fluorescing under a UV-light. The applicability of the test is demonstrated with cucumber mosaic virus and potato virus X
