The frequency of 17 HLA antigens from locus A and locus B has been evaluated by microlymphocytotoxicity test in a population of 233 subjects (157 normal and 76 with deficiency of glucose-6-phosphate ...dehydrogenase G-6PDin red cells) from a village of Sardinia, a mediterranean island relatively close to continental Italy. It appears that G-6-PD-deficient people show a frequency of some HLA antigens (A2, A10, B12, BW35) significatively different from normal Sardinian subjects but close to (A10, BW35) or higher (A2, B12) than that of subjects from peninsular Italy.
The purpose of this study was to describe the morphology of the whole lymphatic way: from capillaries to thoracic duct including cisterna chili using scanning electron microscopy and Evan's ...technique. We observed the lymph vascular wall that is: the endothelial surface, the muscular layer and the adventitial one. All these vessels were covered by an endothelial surface, with raised nuclei and long cell axes oriented parallel to the direction of flow. The borders between adjacent endothelial cell were often seen and open junctions were noted in lymphatic capillaries. The technique we used, permitted the removal of connective tissue by HC1 hydrolysis, so that smooth muscle cells could be examined. The latter showed a great variety of aspects and a very irregular course. The adventitial layer was thin in capillaries and became complex in thoracic duct where collagen fibers and connective elements were seen.
In guinea pig mesenterial lymph vessels there are many bicuspid valves, which determine the flow centralwards of lymph. Our observations, based on the study of 52 guinea pig lymph collectors, ...demonstrated a different number of valves on different parts of each vessel. In fact we found more valves in the part near the intestinal wall and the mesenterial lymph node than in the middle part of the vessel. Besides we measured the time of flow in these different portions by Indian ink injection and we found a correlation between valve number and flow. In fact the time of flow decreases with the increase of the number of valves the shortest being near the intestinal wall and the mesenterial lymph node and the longest being in the middle part of the collectors. The experimental data indicate that the valves have an important role in the lymph circulation because they favour the flow lymph velocity.
