Patients with COVID-19 infection appear to develop virus-induced hypercoagulability resulting in numerous thrombotic events. The aim of the present study was to determine the relationship between the ...thrombophilia genes mutations (prothrombin G20210A, factor V Leiden, and methyltetrahydrofolate reductase (MTHFR)) and the severity of COVID-19 patients.
Prospective cross-sectional study.
One hundred and forty patients (80 adults and 60 children) were included in the current study. They were divided into the severe COVID-19 group and the mild COVID-19 group, with each group comprising 40 adults and 30 children. The patients were assessed for FV R506Q, FV R2H1299R, MTHFR A1298C, MTHFR C677T, and prothrombin gene G20210A polymorphisms. CBC, D-dimer, renal and liver function tests, hs-CRP, ferritin, and LDH were also assessed. Thrombotic events were clinically and radiologically documented.
Severe COVID-19 cases were significantly more frequent to have a heterozygous mutation for all the studied genes compared to mild COVID-19 cases (p<0.05 for all). Being mutant to gene FV R506Q carried the highest risk of developing a severe disease course (p<0.0001). Patients with abnormally high D-dimer levels were significantly more frequent to be heterozygous for FV R506Q, FV R2H1299R, and prothrombin gene G20210A (p = 0.006, 0.007, and 0.02, respectively).
We concluded that there is an evident relationship between severe COVID-19 and inherited thrombophilia. In the current study, FV R506Q gene mutation carried the highest risk of developing a severe COVID-19 disease course.
can cause intestinal and extra-intestinal infections which ranged from mild to life-threatening infections. The severity of infection is a product of many factors including virulence properties and ...antimicrobial resistance.
To determine the antibiotic resistance pattern, the distribution of virulence factors and their association with one another and with some selected resistance genes.
Virulence properties were analyzed phenotypically while antimicrobial susceptibility was tested by Kirby-Bauer agar disc diffusion method. In addition, 64
isolates were tested for 6 colicin genes,
virulence genes and
, and
resistance genes by polymerase chain reaction (PCR).
Extra-intestinal pathogenic
isolated from urine and blood samples represented a battery of virulence factors and resistance genes with a great ability to produce biofilm. Also, a significant association (P<0.05) among most of the tested colicin, virulence and resistance genes was observed. The observed associations indicate the importance and contribution of the tested factors in the establishment and the progress of infection especially with
(ExPEC) which is considered a great challenging health problem.
There is a need for studying how to control these factors to decrease the rate and the severity of infections. The relationship between virulence factors and resistance genes is complex and needs more studies that should be specific for each area.
Herein, the main objective of this research is to design and synthesize a novel optical chemosensor, 2,6-Bis(4-dimethylaminophenyl)-4-(dicyanomethylene)-cyclohexane-1,1-dicarbo-nitrile (BDC), for ...detection of one of the most significant metal ions Cu(II). This novel fluorescent chemosensor exhibits unique optical properties with large Stokes shift (about 170 nm) approximately. The fluorescence and UV–vis absorption performance among the BDC probe and Cu(II) ions were examined in 1:9 (v/v) methanol–HEPES buffer (pH = 7.2) solution. Also, BDC displays high selectivity for Cu(II) concerning other cations. Moreover, this probe provides high selectivity and sensitivity based on their fluorescence properties and recognition abilities within a detection limit of the Cu(II) contents (LOD 2.3 x 10−7 M). The suggested mechanism of BDC sensor is attributed to the chelation process with Cu(II), to establish a 1:1 metal-ligand ratio complex with a binding constant (Kbind = 7.16 x 104 M−1). The detection process is accompanied by quenching the main emission peak of the BDC at 571 nm. All the experimental data were collected to investigate the effects of several important parameters such as reversibility and the concentration limits. Besides, we study the interference of various metal ions on selectivity and detection capacity of this significant Cu (II) ion. This novel chemosensor shows ultrasensitive, fast tracing of Cu(II) in the physiological pH range (pH 7.2) and therefore may propose a novel promising method for the investigation of the biological functions of Cu(II) in living cells.
Purpose:
An increasing number of scholarly publications on autism spectrum disorder (ASD) have urged researcher interest in this topic; however, there is still a lack of quantitative analysis. ...Therefore, this study aims to cover the knowledge gap between the amount of literature published on ASD research on architectural and designers' perspectives compared to the medical and psychological fields. The study has analyzed global research output on ASD from a designer's perspective to recognize this gap related to designing the physical environment.
Methodology:
The bibliometric method was employed to analyze the published literature from 1992-to 2021. 812 papers were downloaded from the Web of Science for analysis based on annual growth of literature, prolific authors, authorship pattern, organizations, countries, international collaboration, and subject development by keywords and thematic map analyses. Various bibliometric and scientometric software was used to analyze the data, namely Bibexcel, Biblioshiny, and VOS viewer.
Results:
The812 research papers were published in 405 sources. 2019 appeared as a productive year (NP=101), and 2014 received the highest number of citations (TC=6634). Researchers preferred to publish as journal articles (NP=538; TC=24922). The University of Toronto, Canada, was identified as a productive institution with 42 publications and 5358 citations. The USA was the leading producing country with 433 publications, and most of the researchers published in the journal "
Scientific Reports" (NP=16). The word autism (NP=257) and architecture (NP=165) were more frequently used keywords.
Conclusion:
The study identified a massive gap in the development of literature in ASD for architecture design and built environment perspective, the most important and trending keywords are missing, and the analyses also showed a lack of subject development. The authors have suggested areas and keywords for further research to fulfill the gap in the future.
Nosocomial infections mainly are due to inefficient cleaning in association with the uncontrollable prescription of antimicrobials resulting in the emergence of multi-drug resistant pathogens in the ...hospital environment. Objectives:The study aims to evaluate the impact of the implementation of culture-guided antibiotic policy with strict infection control strategies on the occurrence of nosocomial infections and the resistance pattern ofthe isolated clinical and environmental pathogens. The study was done in 2 periods. Firstly, (August 2016 – April 2017), routine disinfection procedures and the applied antibiotic policy were evaluated. Secondly, according to the results a new antibiotic policy depending on the culture sensitivity results were implemented starting from June 2017 to February 2018 in association with strict infection control practices. As a result of this intervention, A change in the type of the isolated microorganisms was observed.Antibiotic resistance was decreased. Mortality rate was reduced from 14.1% to 9.5% of neonates with nosocomial infections, the number of the prescribed antibiotics didn’t exceed 4 antibiotics decreasing the overall cost for neonates’ therapy during their hospital stay. Each hospital should have its own antibiotic policy with the application of strict infection control strategies for the control of nosocomial infection.
Objectives
The complex root canal anatomy of primary teeth keeps it very tricky to attain appropriate cleansing by biomechanical instrumentation, so obtaining an obturating material with excellent ...antimicrobial properties is a challenge in current clinical pulpectomy practice. So, this study aimed to assess the clinical and radiographic performance of zinc oxide-ozonated olive oil as a primary root canal filling material.
Materials and methods
Ninety non-vital primary molars in children ranging from 4 to 8 years were allocated into three groups in which root canals were filled with zinc oxide-ozonated olive oil, zinc oxide-olive oil, and zinc oxide-eugenol (ZOE) according to each group after pulpectomy procedure. Clinical and radiographic evaluations were done at 3-, 6-, and 12-month follow-up periods. Statistical analysis was performed for the collected data.
Results
All study groups showed a significant improvement regarding clinical signs and symptoms during follow-up periods. Ozonated-olive oil group revealed a significant increase in furcation radiodensity and a decrease in periodontal ligament space at 3-, 6-, and 12-month follow-up intervals compared to other groups.
Conclusion
Zinc oxide-ozonated olive oil and zinc oxide-olive oil paste had shown good clinical and radiographic success for primary teeth pulpectomy.
Clinical relevance
The intricate torturous primary root canal anatomy, in addition to the child’s negative behavior, interferes with the complete debridement, so the long-lasting antibacterial effect of the primary root canal filling material aids in the pulpectomy success.
In this work, a novel optical fluoro-chemisensor was designed and synthesized for copper (II) ions detection. The sensor film is created by embedded N,N-Bis(2-hydroxo-5-bromobenzyl)ethylenediamine in ...poly vinyl chloride (PVC) film in presence of dioctyl phthalate (DOP) as plasticizer. The receptor Schiff base reveals “off-on” mode with high selectivity, significant sensitivity to Cu(II) ions. The selectivity of optical sensor for Cu(II) ions is the result of chelation enhanced fluorescence (CHEF). The optimal conditions of pH and response time at which higher efficiency of sensor film is performed was found to be 6.8 and 2.48min. The possible interference of other metal ions in solution was examined in presence of different types of metal ions. This film shows high selectivity and ultra-sensitivity with low detection limit LOD (1.1×10−8M). Thus, these considerable properties make it viable to monitor copper metal ions within very low concentration range (0–15×10−6M Cu(II)) and highly selective even in the presence of different types of metal ions. The sensor reversibility was achieved by utilizing EDTA solution with concentration of 0.1M solution.
A novel optical fluoro-chemisensor was designed and synthesized for copper (II) ions detection. The sensor film is created based on the embedded of N,N-Bis(2-hydroxo-5-bromobenzyl)ethylenediamine in poly-vinyl chloride (PVC) matrix. The optimal conditions of pH and response time at which higher efficiency of sensor film is performed were found to be 6.8 and 2.48min respectively. This film shows high selectivity and ultra-sensitivity with low detection limit of 7.6nM. Display omitted
•New chemi-sensor film based Schiff base for Cu(II) detection was constructed.•The detection mechanism based on (CHEF)•The sensor film is easy to applied in aqueous solution.•With high sensitivity and selectivity
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•New chemosensor film based DTD chemical probe for detecting Hg(III) was constructed.•The detection mechanism based on intramolecular charge transfer (ICT).•The DTD chemosensor ...exhibits high sensitivity and selectivity for Hg(II) detection.•DFT and TDDFT theoretical calculations were utilized to examine the formed complex.
we develop and design a novel chemosensor film for detecting Hg(II) ions. The determination process investigates within a concentration range between 0 and 0.10 µM. The optical chemosensor film is depending on 3,4-Dimethylthieno2,3-bthiophene-2,5-Dihydrazide (DTD). The fluorescent molecule emits a significant fluorescence emission at 430 nm under 310 nm. Moreover, the chelation process of the Hg(II) ions with DTD introduces a 2:1 (metal:ligand) complex which is escorted by the quenching of the maximum emission peak centered at 430 nm of DTD and the appearance of a second fluorescence peak at 475 nm. The ratiometric fluorescence influence is a characteristic proof for the complexation reaction between the DTD probe and Hg(II) and this could be easily noticed. The designed chemosensor was proposed depending on the significant fluorescence mechanism, (intramolecular charge transfer (ICT)). Density functional theory (DFT) and the time-dependent DFT (TDDFT) theoretical calculations were achieved to investigate Hg(II) complexation structures and their electronic characteristics in solution, the calculations support that the quenching of the fluorescence in this compound is the result of ICT. This chemosensor displays ultimate sensitivity and selectivity based on their significant fluorescence characteristics, distinctive Stocks shift ~120 nm, detection aptitudes within a substantial low detection limit for Hg(II) LOD 2.4 nM which is applied in a physiological pH medium (pH = 7.4); with a relative standard deviation RSDr (1%, n = 3). Furthermore, the DTD exhibits great binding attendance towards Hg(II) and the binding constant was calculated to be 1.02 × 106 M−1. Impressively, we introduce a significant optical sensor based on the DTD molecule.
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