A series of novelpyridine-connected piperidine derivatives (2a-g) and pyridine-connected 2H-thiopyran derivatives (4a-g) were synthesized and screened for larvicidal, nematicidal, and antimicrobial ...activities. Compound 4e exhibited larvicidal activity against second instar larvae with an LD50 value of 0.8μg/mL. In addition, 4e exhibited high nematicidal activity, with an LD50 value of 3.2μg/mL.Compounds 2e (MIC: 4 μg/mL) and 2d (MIC: 4 μg/mL) exhibited high antibacterial activity against Klebsiella pneumoniae and Escherichia coli, respectively. Compounds 4b (MIC: 0.25 μg/mL) and 4f (MIC: 2 μg/mL) showed high antifungal activity against Candida albicans and Microsporum audouinii, respectively. Therefore, it can be suggested that compounds 2e, 2d, 4e, 4b, and 4f may be useful as lead molecules for the development of new classes ofdrugs with larvicidal,nematicidal,and antimicrobial activities.
Crop development is used to address environmental problems in the industry. Environmental pollutants, particularly humble or toxic chemicals and dyes, are contaminants in industrial wastewater. ...Recently, green technology has become important for controlling the chemical risks associated with environmental issues. This study focused on valuable crop materials to form green catalysts that can be used to control chemical toxicity in industrial wastewater. In this study, Musa acuminata-free tepal (MAFT) crops and musa were used to mediate the formation of copper nanocatalyst for environmental pollution. The synthesised nanocatalyst was analysed by XPS, XRD, TEM, SEM, FT-IR, GC-EI-MS, and TGA-DSC. The production of Cu–O in the 512 cm−1 peak range was verified by FT-IR analysis. The XRD peaks associated with the cubic crystalline structures of copper (110) and (200) were used to confirm the Musa acuminata-free tepal CuO nanocatalyst. The SEM images of the Cu and CuO nanocatalyst in the Musa acuminata free tepal showed that the particles were almost spherical. The size of the synthesised Musa acuminata free tepal CuO nanocatalyst core-shell nanostructures was identified using HR-TEM. GC-MS investigations also verified the aerobic oxidation of the main alcohol to aldehyde, with a retention time of 30.34. The MAFT was 80 % of CBB G250 dye decolourised at 24 h, and the MAFT-CuO nanocatalyst was decolourised the CBB G250 dye at 80 % in 20 min and aerobic oxidation at the same time (dual application). Furthermore, our nanocatalyst could be effectively recycled without significant loss of catalytic activity, and the use of safe, environmentally benign MAFT-Cu and CuO nanocatalyst is considered a green chemistry approach that helps avoid wastewater treatment. MAFT-Cu and CuO nanocatalyst were successfully synthesised using Musa acuminata free tepal extract assisted by reflux sample preparation system instruments as a new, green, eco-friendly, and fast process for nanocatalyst synthesis. In the past, there have been no reported applications of MAFT-Cu and CuO nanocatalyst for decolourisation and aerobic oxidation. Nevertheless, we were able to accomplish these procedures with the help of CuO and MAFT-Cu nanocatalyst. Therefore, the most effective naturally occurring environmental catalysts for aerobic oxidation processes are MAFT-CuO nanocatalyst. They are non-toxic and environmentally benign, exhibit strong aerobic oxidation catalytic performance, and have long-lasting benefits for water quality. Additionally, they are more recoverable and biodegradable.
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•Collection of Musa acuminata free tepal crop and Synthesis of Musa acuminata free tepal crop with CuO NPs (MAFT-CuO).•Characterisation of FT-IR, UV-Visible spectroscopy, XRD, XPS, TEM, SEM, GC-MS, and TGA, etc.•Aerobic Oxidation of alcohol dehydration and Dye Decolourization via eco-friendly method.•Musa acuminata free tepal crop used for dye decolouration.•MAFT-CuO used for dual application in dye decolouration and aerobic oxidation.
In this study, we developed a novel pyrazolo3,4-
pyrazole derivative with antibacterial and antifungal activities that shows great potential for treating infectious diseases. To evaluate the binding ...affinity of 1AJ0 and 1AI9 proteins for developing potent antibacterial and antifungal compounds, we used the
(
) leaf extract as the capping and reducing agent and reacted it with Fe
O
and Cu(OAc)
solutions to synthesize the
-Fe
O
-CuO nanocatalyst. The newly synthesized compounds were confirmed using Fourier transform infrared spectroscopy, transmission electron microscopy, UV-visible spectroscopy, and X-ray diffraction analyses. Antibacterial screening revealed that compound 1g was highly active against
(MIC: 1 μg mL
) and was much more effective than the standard ciprofloxacin. Compound 1b showed a higher antifungal activity than clotrimazole against
(MIC: 0.25 μg mL
) and cytotoxic activity against MCF-7 cancer cell lines. Compounds 1a-1l were exhibited low cytotoxicity activity compared to the standard doxorubicin (LC
: 21.05 ± 0.82 μg mL
). To further support the discovery of new active antibacterial agents, compounds 1g and 1b and proteins 1AJ0 and 1AI9 were examined using the AutoDock Vina program and were compared with the standards ciprofloxacin and clotrimazole. With the 1AJ0 protein, compound 1g had a higher docking score (-3.7 kcal mol
) than ciprofloxacin (-5.6 kcal mol
), and with the 1AI9 protein, compound 1b had a higher docking score (-4.8 kcal mol
) than clotrimazole (-4.4 kcal mol
). Additionally, molecular dynamics simulation was used to investigate the most probable binding mode of compounds 1b and 1g with 1AI9 and 1AJ0, respectively. The
-Fe
O
-CuO catalyst was used to prepare pyrazolo3,4-
pyrazole derivatives, which were successfully characterized and screened for antimicrobial and cytotoxic activities, molecular docking, and molecular dynamics simulation studies.
The phytochemical constituent and antimicrobial activities of Bersama abyssinica leaf extracts against multidrug-resistant bacterial uropathogens were evaluated. Seven organic solvents were used in ...the extraction of the leaves of B. abyssinica. Agar well diffusion assay, minimum inhibitory and minimum bactericidal concentration analysis, bioassay-guided fractionation, and spectral and elemental analysis were used to identify the principal phytochemical ingredients. Confirmation of detected compound was done by thin layer chromatography, elemental and spectroscopic (
1
H-NMR,
13
C NMR, and IR) techniques. Ethyl acetate-based extract demonstrated the highest antimicrobial activity against the tested bacteria. The highest zone of inhibition (395.3 ± 26.7 mm
2
) was observed by the ethyl acetate extract against multidrug-resistant uropathogenic Staphylococcus aureus. Totally, 11 main compounds were identified from the active fraction of which picolinyl hydrazide was the principal compound. Antimicrobial activities of B. abyssinica varied with the extraction solvents, concentration of extracts, and types of test microorganisms. Gram-positive bacteria were more susceptible to the plant extracts, and ethyl acetate was the best extraction solvent.
In this study, we developed a novel pyrazolo3,4-
c
pyrazole derivative with antibacterial and antifungal activities that shows great potential for treating infectious diseases. To evaluate the ...binding affinity of
1AJ0
and
1AI9
proteins for developing potent antibacterial and antifungal compounds, we used the
Vitex negundo
(
VN
) leaf extract as the capping and reducing agent and reacted it with Fe
2
O
3
and Cu(OAc)
2
solutions to synthesize the
VN
-Fe
3
O
4
-CuO nanocatalyst. The newly synthesized compounds were confirmed using Fourier transform infrared spectroscopy, transmission electron microscopy, UV-visible spectroscopy, and X-ray diffraction analyses. Antibacterial screening revealed that compound
1g
was highly active against
Escherichia coli
(MIC: 1 μg mL
−1
) and was much more effective than the standard ciprofloxacin. Compound
1b
showed a higher antifungal activity than clotrimazole against
Candida albicans
(MIC: 0.25 μg mL
−1
) and cytotoxic activity against MCF-7 cancer cell lines. Compounds
1a-1l
were exhibited low cytotoxicity activity compared to the standard doxorubicin (LC
50
: 21.05 ± 0.82 μg mL
−1
). To further support the discovery of new active antibacterial agents, compounds
1g
and
1b
and proteins
1AJ0
and
1AI9
were examined using the AutoDock Vina program and were compared with the standards ciprofloxacin and clotrimazole. With the
1AJ0
protein, compound
1g
had a higher docking score (−3.7 kcal mol
−1
) than ciprofloxacin (−5.6 kcal mol
−1
), and with the
1AI9
protein, compound
1b
had a higher docking score (−4.8 kcal mol
−1
) than clotrimazole (−4.4 kcal mol
−1
). Additionally, molecular dynamics simulation was used to investigate the most probable binding mode of compounds
1b
and
1g
with
1AI9
and
1AJ0
, respectively. The
VN
-Fe
3
O
4
-CuO catalyst was used to prepare pyrazolo3,4-
c
pyrazole derivatives, which were successfully characterized and screened for antimicrobial and cytotoxic activities, molecular docking, and molecular dynamics simulation studies.
In this study, we developed a novel pyrazolo3,4-
c
pyrazole derivative with antibacterial and antifungal activities that shows great potential for treating infectious diseases.
In addition to the physical barrier, the epidermis acts as a natural barrier against microbial proliferation. It is prone to bacterial infections on the skin and in the nose, such as Staphylococcus ...aureus, as well as a variety of other skin illnesses. Green nanomaterial production, which eliminates the use of harmful chemicals while simultaneously reducing time, is gaining popularity in the nanotechnology area. Using the leaf extract of the pharmacologically valuable plant Moringa oleifera, we described a green synthesis of ZnO NPs (zinc oxide nanoparticles). ZnO NPs had a particle size of 201.6 nm and a zeta potential of -56.80 mV, respectively. A novel aminoketone antibacterial medication was synthesized and tested for antibacterial activity using ZnO NPs as a phytocatalyst in this work. This method produces high yields while maintaining efficient and gentle reaction conditions. Moringa oleifera extract can reduce ZnO to ZnO NPs in a straightforward manner. FT-IR,
H-NMR,
C-NMR, mass spectra, elemental analysis, and morphological analysis were used to synthesize and describe the antibacterial medicines (1a-1g) and (2a-2g). In addition, antibacterial activity was evaluated against bacteria such as
and
and compound 1c (63
g/mL,
) and compound 2e (0.12
g/mL,
) were found to be very active when compared to other medications. mupirocin is used as a reference. In addition, studies of in silico molecular docking for the bacterial DsbA protein were conducted. The strong molecules 1c (-4.3 kcal/mol) and 2e (-5.1 kcal/mol) exhibit a high binding affinity through hydrogen bonding, according to docking tests.
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•First time, we reported novel morpholine-connected pyrazolidine derivatives.•Easy way to synthesis.•Using metal-free catalytic of pyrrolidine compounds.•High yield.•High significant ...of antimicrobial, antioxidant and cytotoxic activities.
A simple and convenient one-pot four-component synthesis of morpholine-connected pyrazolidine derivatives 2a–f and 4a–f was developed using direct metal-free catalysis, with the identities of the synthesized compounds confirmed by IR, NMR (1H and 13C), mass spectrometry, and elemental analysis. The prepared compounds were inspected for antimicrobial, antioxidant, and cytotoxic activities.
Antimicrobial and antifungal activities against five bacterial and four fungal pathogens, respectively, were investigated using the disc diffusion technique. In antibacterial activity, compounds 2d and 2f (MIC=2μg/mL) exhibited significantly higher activity than the standard ciprofloxacin. The results of antifungal assay showed that the activity of compound 4a (MIC=0.5μg/mL) was significantly higher than the standard clotrimazole. Antioxidant activity was screened based on ABTS+ radical scavenging and linoleic acid peroxidation performance. Compound 4a showed substantial antioxidant (91.3%) activities, as compared with the Trolox standard. Cytotoxicity was evaluated using HepG2 (liver), HeLa (cervical), and MCF-7 (breast) cancer cell lines, with high toxicities observed for 2b (GI50=12.2μm) and 4a (GI50=07.8μm).
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