Objective. In this study the biomass of the yeast Saccharomyces cerevisiae was used to remove lead, mercury and nickel in the form of ions dissolved in water. Materials and methods. Synthetic ...solutions were prepared containing the three heavy metals, which were put in contact with viable microorganisms at different conditions of pH, temperature, aeration and agitation. Results. Both individual variables and the interaction effects influenced the biosorption process. Throughout the experimental framework it was observed that the biomass of Saccharomyces cerevisiae removed a higher percentage of lead (86.4%) as compared to mercury and nickel (69.7 and 47.8% respectively). When the pH was set at a value of 5 the effect was positive for all three metals. Conclusions. pH was the variable that had a greater influence on the biosorption of lead on the biomass of Saccharomyces cerevisiae. The affinity of the heavy metals for the biomass followed the order Pb>Hg>Ni. Key words: Bioremediation, heavy metals, biomass, contaminant removal, bioaccumulation (Source: DECS). Objetivo. En este estudio se utilizo la biomasa de la levadura Saccharomyces cerevisiae para retener plomo, mercurio y niquel en forma de iones disueltos en agua. Materiales y metodos. Se prepararon soluciones sinteticas que contenian los tres metales pesados, las cuales se pusieron en contacto con el microorganismo en forma viable a diferentes condiciones de pH, temperatura, aireacion y agitacion. Resultados. Tanto las variables individuales como los efectos de interaccion influyeron sobre el proceso de biosorcion. A traves de todos los experimentos, se observo que la biomasa de Saccharomyces cerevisiae elimino un mayor porcentaje de plomo (86.4%) en comparacion al mercurio y al niquel (69.7 y 47.8% respectivamente). Cuando el pH se fijo en valor de 5, el efecto fue positivo para los tres metales. Conclusiones. El pH fue la variable que tuvo una mayor influencia en la biosorcion de plomo sobre la biomasa de Saccharomyces cerevisiae. La afinidad de los metales pesados por la biomasa siguio el orden Pb>Hg>Ni. Palabras clave: Biorremediacion, metales pesados, biomasa, remocion de contaminantes, bioacumulacion (Fuente: DECS).
The liver is the most frequent site of metastases from colon cancer. To improve our knowledge of liver metastases and to develop new adjuvant therapies, a good animal model is necessary. The aims of ...this study were to obtain a model of liver metastases with intraportal injection of colon adrenocarcinoma cell aggregates (DHDK12 cell line) and to study the effect of various factors, i.e., sex, liver regeneration and immunosuppression, on the development of liver metastasis. Cell aggregates were injected into the portal vein of 59 syngenic male and female BD IX rats following randomization into three groups. Group 1, (control 12 males and 10 females) received only cell aggregates; group 2 (12 males and 10 females) underwent a 70% hepatectomy before cell injection; group 3 (15 males and 10 females) received cyclosporin A injections at a dose of 10 mg/kg per day for 28 days following cell injection. Autopsy was performed at 10 weeks. Liver metastases were more frequent in the male rats in group 3 than in those in group 1 (80% vs. 30%, p less than 0.04). The rate of liver metastases in females was not increased by immunosuppression (22.2% vs. 12.5%, N.S.). Liver resection (group 2) did not significantly modify the incidence of liver metastasis. No female had liver metastases in this group. This relatively simple model rapidly produces liver metastasis with a high yield, but only in male rats. Besides sexual factors, immunosuppression also increased the rate of experimental liver metastasis, while liver regeneration failed to do so.
We present the characterization of a new mouse cell surface protein, recognized by the 3E8-specific monoclonal antibody. The expression of this antigen is predominantly restricted to the ...hematopoietic and lymphoid tissues: bone marrow, spleen, lymph node, and thymus. Immunoblot analyses show that the 3E8 determinant is present on molecules with different apparent relative masses. The 3E8 antigen migrates on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a single band of Mr 115,000 for normal nonstimulated spleen cells and thymocytes and as two bands of Mr 115,000 and Mr 125,000 for bone marrow cells and mitogen-stimulated spleen cells. The multiple sizes of the 3E8 antigens (isoforms) found on various cell lines are not due to allelic polymorphism, but instead may reflect the specific cell type or reflect the cell's state of activation or maturation. Results from lectin chromatography and N-glycanase and neuraminidase studies suggest that the 3E8 antigen is a heavily sialylated O-linked glycoprotein. The unusual features of this antigen indicate that it may be the mouse homologue of the rat W3/13 antigen and the human leukosialin/sialophorin antigens.
Immunization of C57BL/6 mice with purified acetylcholine receptor (AChR) is known to induce a T cell-dependent antibody response that results in experimental autoimmune myasthenia gravis (EAMG). ...Since past observations link V beta 6+ T cells with a prominent AChR epitope specificity, a V beta 6-specific immunotoxin (VIT6) was tested in vitro for its ability to selectively kill monoclonal and polyclonal T cells that demonstrate reactivity against AChR. Results described below clearly demonstrate the ability to selectively kill AChR-reactive T cells based on their expression of a particular V beta-associated antigen receptor.
Biliary hydatidosis Molina Infante, J.; Fernández Bermejo, M.; Martín Noguerol, E. ...
Revista española de enfermedades digestivas,
02/2009, Letnik:
101, Številka:
2
Journal Article
To assess the clinical utility of PERCIST and Hopkins criteria and changes in 18FFDG PET/CT quantitative parameters as prognostic factors for progression-free survival (PFS) and cancer-specific ...survival (CSS) in patients with head and neck squamous cell carcinoma treated by chemoradiotherapy.
Forty patients (34 men) diagnosed with head and neck squamous cell carcinoma were retrospectively assessed over an interval of 8 years. PERCIST and Hopkins criteria were used to assess response to treatment. Variations in the metabolic parameters maximum SUV (ΔSUVmax), metabolic tumor volume (ΔMTV) and total lesion glycolysis (ΔTLG) between pre- and post-treatment PET/CT studies were also determined. The Cox regression model, ROC curves and the Kaplan–Meier method were used for the analysis of prognostic factors and survival curves.
The mean follow-up was 39.4 months, with 24 progressions and 22 deaths. Both PERCIST and Hopkins criteria and the three metabolic parameters were predictive factors in the univariate analysis and only ΔSUVmax was in the multivariate analysis. Survival analysis showed statistically significant differences in PFS and CSS curves for the five parameters considered.
Application of PERCIST and Hopkins criteria as well as ΔSUVmax, ΔMTV and ΔTLG from PET/CT studies proved to be prognostic factors for survival in patients in our setting for treating head and neck cancer. The results may help to personalize treatment.
Valorar la utilidad clínica de los criterios PERCIST, Hopkins y de los cambios en los parámetros cuantitativos de la PET/TC con 18FFDG como factores pronósticos para la supervivencia libre de progresión (SLP) y la supervivencia cáncer específica (SCE) en pacientes con cáncer escamoso de cabeza y cuello tratados mediante quimiorradioterapia.
Se valoraron retrospectivamente 40 pacientes (34 hombres) diagnosticados de cáncer escamoso de cabeza y cuello durante un intervalo de 8 años. Se utilizaron los criterios PERCIST y Hopkins para determinar la respuesta al tratamiento. Asimismo, se cuantificaron las variaciones de los parámetros metabólicos SUV máximo (ΔSUVmax), volumen metabólico tumoral (ΔMTV) y glicólisis tumoral total (ΔTLG) entre los estudios PET/TC pre y postratamiento. El modelo de regresión de Cox, las curvas ROC y el método de Kaplan–Meier y se aplicaron para el análisis de factores pronósticos y curvas de supervivencia.
El seguimiento medio fue de 39,4 meses produciéndose 24 recidivas-progresiones y 22 muertes. Tanto los criterios PERCIST y Hopkins como los tres parámetros metabólicos fueron factores predictivos en análisis univariante y solo el ΔSUVmax en el multivariante. El análisis de supervivencia mostró curvas de SLP y SCE significativamente diferentes para los cinco parámetros considerados.
La aplicación de los criterios PERCIST y Hopkins, así como los ΔSUVmax, ΔMTV y ΔTLG de los estudios PET/TC demostraron ser factores pronósticos para la supervivencia en pacientes de nuestro entorno tratados por cáncer de cabeza y cuello. Los resultados podrían ayudar a personalizar el tratamiento.
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