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•We reviewed materials and functionalization strategies to engineer nanobiocatalysts.•Coordination between materials and enzymes play a critical role in developing ...nanobiocatalysts.•The characteristic properties rendering materials interesting matrices for immobilization.•Functionalized constructs can be used as immobilization carriers for enzymes.
Suitable coordination between the new wave of nanostructured materials and catalyst of interests play a critical role in developing nanobiocatalysts with new or improved functionalities. In this context, enzymes with natural origin are versatile biocatalysts with multifunctional characteristics and have been widely utilized in various sectors such as environmental, energy, biomedical, pharmaceutical, cosmeceutical, nutraceutical, fine chemicals, agro-industrial, and food industry, etc. The deployment of enzymes in a non-natural environment has limited boundaries such as the high production cost, challenging separation, purification, and liability to deactivation under non-ambient conditions. These drawbacks can be overcome by the design and fabrication of novel hybrid and functionalized nanobiocatalyst. However, appropriate coordination at chemical, physical, and the biological level is highly requisite to engineer such nanobiocatalysts of supreme interests. Currently, the generation and development of diverse nanomaterials along with new strategies have been established from the nanotechnology perspectives, where the integration of naturally occurring biocatalysts with suitable nanomaterials offer an exceptional corridor to upgrade the catalytic performances of pristine enzymes. Recent innovations in nano-biotechnology furnished numerous opportunities to integrate natural biocatalysts to a range of nanostructured materials with unique attributes. These newly introduced nanomaterials show/impart additional characteristics which enzyme in their pristine form fails to demonstrate on their own. Manipulation of these nanomaterials for enzyme delivery or recovery, remote access for activation or deactivation of enzymatic activity, and new catalytic entities with harmonizing functionalities has taken this field to a new horizon with pronounced biotechnological applications in the coming years. The present review emphases on the recent developments along with the exploitation of nanostructured materials including nanofibers, hybrid nanoflowers, mesoporous/nanoporous carriers, carbon nanotubes, magnetic or non-magnetic nanoparticles, and nanocomposites as support carriers for the immobilization of different enzymes to develop nanobiocatalysts with potential activity and stability characteristics. In addition, strategies for the synthesis and various types of new functionalization approaches, particularly the chemical method for its capability to modify nanomaterials with enormous functionalities are discussed. Towards the end, challenges related to the use of nanobiocatalysts and their possible solution are summarized.
In recent years, emerging contaminants (ECs) of high concern are broadly distributed throughout the environmental matrices because of various industrial practices and anthropogenic inputs, i.e., ...human-made activities. With ever increasing scientific knowledge, technological advancement, socio-economic awareness, people are now more concern about the widespread distribution of environmentally related ECs of high concern. As, ECs possess serious ecological threats and potential risks to human health and aquatic life, even at minor concentrations. The controlled or uncontrolled discharge and long-term persistence of ECs that includes micro-pollutants, endocrine disruptors (EDs), pesticides, pharmaceuticals, hormones, toxins, and industrially-related synthetic dyes and dyes-containing hazardous pollutants, etc. pose a significant challenge to policy regulators, engineers, and scientific community. The conventional treatment technologies are proved ineffective for the complete elimination and removal of an array of contaminants of emerging environmental concern in various biological and environmental samples. In order to overcome the aforementioned ecological threats, tremendous research efforts have been made to boost the efficiency of remediation techniques or develop new modalities to detect, quantify and treat the samples efficiently. The boom in biotechnology and environmental engineering offers potential opportunities to develop advanced and innovative remediation techniques in the field of water treatment. This review discusses the environmental and health hazards associated with a widespread distribution of micro-pollutants, pesticides, pharmaceuticals, hormones, and industrially-related synthetic dyes and dyes-containing hazardous pollutants, etc. in the water bodies, i.e., surface water, groundwater, and industrial wastewater streams. Life-cycle distribution of emerging (micro)-pollutants with suitable examples from various industrial sources viewpoints is also discussed. The later part of the review focuses on innovative and cost-effective remediation (removal) approaches from phase-changing treatment technologies for these ECs of high concern.
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•Herein, we reviewed environmentally-related emerging contaminants (ECs) of high concern.•Potential ECs sources and analytical modalities for detection, quantification, and treatment are discussed.•Significant actions are urgently required to tackle/control the ECs in the environment.
The widespread occurrence and adverse environmental and health-related impacts of various types of emerging contaminants (ECs) have become an issue of high concern. With ever increasing scientific ...knowledge, socio-economic awareness, health-related problems and ecological apprehensions, people are more concerned about the widespread ECs, around the globe. Among ECs, biologically active compounds from pharmaceutical, cosmeceutical, biomedical, personal care products (PPCPs), endocrine-disrupting chemicals (EDCs), and flame-retardants are of paramount concern. The presence and persistence of ECs in water bodies are of continued and burning interest, worldwide. Various types of ECs are being discharged knowingly/unknowingly with/without partial treatments into the aquatic environments that pose serious health issues and affects the entire living ecosystem. So far, various approaches have been developed for ECs degradation and removal to diminish their adverse impact. Many previous and/or ongoing studies have focused on contaminants degradation and efficient removal via numerous treatment strategies, i.e. (1) physical, (2) chemical and (3) biological. However, the experimental evidence is lacking to enable specific predictions about ECs mechanistic degradation and removal fate across various in-practice systems. In this context, the deployment oxidoreductases such as peroxidases (lignin peroxidases, manganese-dependent peroxidases, and horseradish peroxidase), aromatic dioxygenases, various oxygenases, laccases, and tyrosinases have received considerable research attention. Immobilization is highlighted as a promising approach to improve enzyme catalytic performance and stabilization, as well as, to protect the three-dimensional structure of the enzyme against the undesirable consequences of harsh reaction environment. This work overviews the current and state-of-the-art critical aspect related to hazardous pollutants at large and ECs in particular by the immobilized oxidoreductase enzymes. The first part of the review focuses on the occurrence, physiochemical behavior, potent sources and significant routes of ECs. Following that, environmentally-related adverse impacts and health-related issues of ECs are discussed in the second part. In the third part, biodegradation and removal strategies with a comparative overview of several conventional vs. non-conventional methods are presented briefly. The fourth part majorly focuses on operational modes of different oxidoreductase enzyme-based biocatalytic processes for the biodegradation and biotransformation of a wide array of harmful environmental contaminants. Finally, the left behind research gaps, concluding remarks as well as future trends and recommendations in the use of carrier-immobilized oxidoreductases for environmental perspective are also discussed.
•This review deals with the removal of emerging contaminants (ECs) by immobilized oxidoreductases.•Occurrence, physicochemical behavior, potent sources and significant routes of ECs are discussed.•Environmentally-related adverse impacts and health-related issues of ECs are discussed.
Industries are the paramount driving force for the economic and technological development of society. However, the flourishing industrialization and unimpeded growth of current production unit's ...result in widespread environmental pollution due to increased discharge of wastes loaded with baleful, hazardous, and carcinogenic contaminants. Physicochemical-based remediation means are costly, create a secondary disposal problem and remain inadequate for pollution mitigating because of the continuous emergence of new recalcitrant pollutants. Due to eco-friendly, social acceptance, and lesser health hazards, microbial bioremediation has received considerable global attention for pollution abatement. Moreover, with the recent advancement in biotechnology and microbiology, genetically engineered bacteria with high ability to remove environmental pollutants are widely used in the fields of environmental restoration, resulting in the bioremediation in a more viable and eco-friendly way. This review summarized the advantages of genetically engineered bacteria and their application in the treatment of a wide variety of environmental contaminants such as synthetic dyestuff, heavy metal, petroleum hydrocarbons, polychlorinated biphenyls, phenazines and agricultural chemicals which will include herbicides, pesticides, and fertilizers. Considering the risk of genetic material exchange by using genetically engineered bacteria, the challenges and limitations associated with the application of recombinant bacteria on contaminated sites are also discussed. An integrated microbiological, biological and ecological acquaintance accompanied by field engineering designs are the desired features for effective in situ bioremediation of hazardous waste polluted sites by recombinant bacteria.
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•This review highlights the mitigation of pollutants by genetically engineered bacteria.•Advantages of engineered bacteria and their applications are discussed.•The challenges and limitations associated with the recombinant bacteria are also discussed.
Engineering enzymes with improved catalytic properties in non-natural environments have been concerned with their diverse industrial and biotechnological applications. Immobilization represents a ...promising but straightforward route, and immobilized biocatalysts often display higher activities and stabilities compared to free enzymes. Owing to their unique physicochemical characteristics, including the high-specific surface area, exceptional chemical, electrical, and mechanical properties, efficient enzyme loading, and multivalent functionalization, nano-based materials are postulated as suitable carriers for biomolecules or enzyme immobilization. Enzymes immobilized on nanomaterial-based supports are more robust, stable, and recoverable than their pristine counterparts, and are even used for continuous catalytic processes. Furthermore, the unique intrinsic properties of nanomaterials, particularly nanoparticles, also confer the immobilized enzymes to be used for their broader applications. Herein, an effort has been made to present novel potentialities of multi-point enzyme immobilization in the current biotechnological sector. Various nano-based platforms for enzyme/biomolecule immobilization are discussed in the second part of the review. In summary, recent developments in the use of nanomaterials as new carriers to construct robust nano-biocatalytic systems are reviewed, and future trends are pointed out in this article.
The progressive increase in the earth's temperature due to anthropogenic activities is a major concern for humanity. The ensuing heat stress (HS) severely impacts plant growth, endangering ecosystem ...quality and world food security. Plant growth, physiological processes and final amount of edible products are affected by HS to an extent that reflects the physical damages, physiological commotions and biochemical alterations incurred at various growth stages. Therefore, a better understanding of plant behaviour in response to HS has pragmatic implications for devising counter-measures, alleviation strategies, and for acknowledging the differences between HS and the companion drought stress. Conventional breeding, biotechnological and molecular approaches are used to develop HS tolerant genotypes in plant species bred for food/feed uses. Recent achievements in the omics techniques result in a better knowledge of the molecular mechanisms involved in HS. However, shrewd management of crop practices is still helpful to improve plant resilience to HS. Suitable sowing time, seed priming, bacterial seed treatment, nutrient and water management, exogenous application of osmo-protectants, and conservation of soil moisture are important tools to improve plant behaviour under the critical HS scenarios determined by climate change and global warming.
The massive consumption of a wide range plastic products has generated a huge amount of plastic waste. There is a need to provide awareness of their uses and routine management as a part of our ...lifestyle. Nowadays, plastics are increasingly being used in our daily life activities, including the packaging in different food and brewing companies, cosmetics, pharmaceutical, and other production sectors need to pack their end products for efficient and safer product's delivery to the community. Plastics are produced through the biochemical process of polymerization or polycondensation. The post-use of generated plastic waste has many adverse impacts on the environment if not processed and managed in a proper way. This review aims to discuss the lifecycle of plastic products according to their different categories, including polyvinyl chloride (PVC–U), polystyrene or styrofoam (PS), polypropylene (PP), high-density polyethylene (HDPE), polyethylene terephthalate (PETE), and others. Herein, we have also discussed the problems caused by the inadequate processing of plastic waste and the possible solutions that can be provided to ensure a good atmosphere and to reduce the causes of climate changes, which is challenging to life on this planet.
Heavy metals are discharged into aquatic environment and causes serious problems to the environment, human's health, and other organisms. The industrial effluents contain high concentration of heavy ...metals that should be treated by different technologies. Numerous technologies have been widely used for the remediation of heavy metals such as chemical precipitation, ion exchange, membrane filtration, adsorption, coagulation-flocculation, floatation, electrochemical treatment, bioremediation, and photocatalysis. Among these technologies, photocatalysis has gained much attention due to chemical, physical, and electrical properties of heterogeneous semiconductor nano-photocatalysis. Bismuth vanadate is an n-type semiconductor photocatalyst having 2.4 eV band gap that was widely used from several decades having three monoclinic, tetragonal, and tetragonal zircon structures, but it also have some limitation that can be overcome by modification with metals or non-metals to gain high removal efficiency of heavy metals. This modification can tune its photocatalytic properties like band gap, absorption capacity, and surface area resulting in high photocatalytic performance towards heavy metals detoxification.
In this study, we reviewed state-of-the-art endogenous-based and exogenous-based stimuli-responsive drug delivery systems (DDS) for programmed site-specific release to overcome the drawbacks of ...conventional therapeutic modalities. This particular work focuses on the smart chemistry and mechanism of action aspects of several types of stimuli-responsive polymeric carriers that play a crucial role in extracellular and intracellular sections of diseased tissues or cells. With ever increasing scientific knowledge and awareness, research is underway around the globe to design new types of stimuli (external/internal) responsive polymeric carriers for biotechnological applications at large and biomedical and/or pharmaceutical applications, in particular. Both external/internal and even dual/multi-responsive behavior of polymeric carriers is considered an essential element of engineering so-called 'smart' DDS, which controls the effective and efficient dose loading, sustained release, individual variability, and targeted permeability in a sophisticated manner. So far, an array of DDS has been proposed, developed, and implemented. For instance, redox, pH, temperature, photo/light, magnetic, ultrasound, and electrical responsive DDS and/or all in all dual/dual/multi-responsive DDS (combination or two or more from any of the above). Despite the massive advancement in DDS arena, there are still many challenging concerns that remain to be addressed to cover the research gap. In this context, herein, an effort has been made to highlight those concerning issues to cover up the literature gap. Thus, the emphasis was given to the drug release mechanism and applications of endogenous and exogenous based stimuli-responsive DDS in the clinical settings.
Lignin peroxidase (LiP) seems to be a catalyst for cleaving high-redox potential non-phenolic compounds with an oxidative cleavage of CC and COC bonds. LiP has been picked to seek a practical and ...cost-effective alternative to the sustainable mitigation of diverse environmental contaminants. LiP has been an outstanding tool for catalytic cleaning and efficient mitigation of environmental pollutants, including lignin, lignin derivatives, dyes, endocrine-disrupting compounds (EDCs), and persistent organic pollutants (POPs) for the past couple of decades. The extended deployment of LiP has proved to be a promising method for catalyzing these environmentally related hazardous pollutants of supreme interest. The advantageous potential and capabilities to act at different pH and thermostability offer its working tendencies in extended environmental engineering applications. Such advantages led to the emerging demand for LiP and increasing requirements in industrial and biotechnological sectors. The multitude of the ability attributed to LiP is triggered by its stability in xenobiotic and non-phenolic compound degradation. However, over the decades, the catalytic activity of LiP has been continuing in focus enormously towards catalytic functionalities over the available physiochemical, conventional, catalyst mediated technology for catalyzing such molecules. To cover this literature gap, this became much more evident to consider the catalytic attributes of LiP. In this review, the existing capabilities of LiP and other competencies have been described with recent updates. Furthermore, numerous recently emerged applications, such as textile effluent treatment, dye decolorization, catalytic elimination of pharmaceutical and EDCs compounds, have been discussed with suitable examples.
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