Applications based on Wireless Sensor Networks for Internet of Things scenarios are on the rise. The multiple possibilities they offer have spread towards previously hard to imagine fields, like ...e-health or human physiological monitoring. An application has been developed for its usage in scenarios where data collection is applied to smart spaces, aiming at its usage in fire fighting and sports. This application has been tested in a gymnasium with real, non-simulated nodes and devices. A Graphic User Interface has been implemented to suggest a series of exercises to improve a sportsman/woman s condition, depending on the context and their profile. This system can be adapted to a wide variety of e-health applications with minimum changes, and the user will interact using different devices, like smart phones, smart watches and/or tablets.
Herein, we describe a series of molecular rotors formed by cocrystallizing three indolo3,2-bcarbazole (ICZ) derivatives with butyl and cyclohexyl substituents, along with ...1,4-diaza2.2.2bicyclooctane (DABCO). The structures of rotors I and III were confirmed through single-crystal X-ray diffraction (SCXRD), revealing a concatenated 1D arrangement between the two components. Variable-temperature (VT) SCXRD experiments on rotors I and III suggested that the rotator shows rotational motion, with activation energies of 6.8 and 1.8 kcal mol–1, respectively. The lower activation energy for rotor III was attributed to the flexible environment around DABCO due to the presence of cyclohexyl groups, while the surroundings of rotor I were found to be more rigid. Additionally, our predictions of radiative and nonradiative decay constants indicate that the vibrations of the molecular rotors impact nonradiative decay rates and, consequently, the fluorescence quantum yields.
Abstract
The 3′-ends of eukaryotic pre-mRNAs are processed in the nucleus by a large multiprotein complex, the cleavage and polyadenylation factor (CPF). CPF cleaves RNA, adds a poly(A) tail and ...signals transcription termination. CPF harbors four enzymatic activities essential for these processes, but how these are coordinated remains poorly understood. Several subunits of CPF, including two protein phosphatases, are also found in the related 'associated with Pta1′ (APT) complex, but the relationship between CPF and APT is unclear. Here, we show that the APT complex is physically distinct from CPF. The 21 kDa Syc1 protein is associated only with APT, and not with CPF, and is therefore the defining subunit of APT. Using ChIP-seq, PAR-CLIP and RNA-seq, we show that Syc1/APT has distinct, but possibly overlapping, functions from those of CPF. Syc1/APT plays a more important role in sn/snoRNA production whereas CPF processes the 3′-ends of protein-coding pre-mRNAs. These results define distinct protein machineries for synthesis of mature eukaryotic protein-coding and non-coding RNAs.
•Hydrogenated Diamond-Like Carbon was successfully deposited on AISI 52100 steel.•The multi-layered DLC significantly improved adhesion with 72.6 N of critical load.•The wear rate behaves as the ...applied load does due to a transferred carbon layer.•Hybrid grey-fuzzy reasoning was used to find optimum real work conditions.
The amorphous carbon (a-C:H) coating, with interlayer architectures improves adhesion significantly. Because of this, a novel multi-layered a-C:H coating was deposited on an AISI 52100 steel, by PECVD. The coating was characterised using SEM-EDS, Raman, nano-indentation, interferometry and pin-on-plate. In addition, a designed experiment was implemented for the tribological tests. Wear and friction results were analysed using a hybrid grey-fuzzy method to obtain optimum combination of the tribo-parameters.
RNA-targeting CRISPR-Cas systems van Beljouw, Sam P B; Sanders, Jasper; Rodríguez-Molina, Alicia ...
Nature reviews. Microbiology,
01/2023, Letnik:
21, Številka:
1
Journal Article
Recenzirano
Odprti dostop
CRISPR-Cas is a widespread adaptive immune system in bacteria and archaea that protects against viral infection by targeting specific invading nucleic acid sequences. Whereas some CRISPR-Cas systems ...sense and cleave viral DNA, type III and type VI CRISPR-Cas systems sense RNA that results from viral transcription and perhaps invasion by RNA viruses. The sequence-specific detection of viral RNA evokes a cell-wide response that typically involves global damage to halt the infection. How can one make sense of an immune strategy that encompasses broad, collateral effects rather than specific, targeted destruction? In this Review, we summarize the current understanding of RNA-targeting CRISPR-Cas systems. We detail the composition and properties of type III and type VI systems, outline the cellular defence processes that are instigated upon viral RNA sensing and describe the biological rationale behind the broad RNA-activated immune responses as an effective strategy to combat viral infection.
Herein, we report two new charge-transfer organic cocrystals, CP-TCNQ and CP-TFBQ. The two cocrystals exhibit broad absorption, good thermal stability, and low photoluminescence quantum yields, the ...necessary requirements to show the photothermal conversion (PTC) effect. Indeed, the two cocrystals exhibit distinct PTC efficiencies of 11.0 ± 1.4% (CP-TCNQ) and 17.0 ± 1.0% (CP-TFBQ). The 55% improvement in the observed efficiencies is attributed to the fast in-plane reorientations of the TFBQ fragment, as determined by detailed ssNMR spectroscopy and DFT calculations. This work provides for the first time experimental and computational evidence that the molecular motion of a cocrystal coformer increases its PTC efficiency, paving the way to the design of new crystalline materials with PTC as a regulated property.
Charge parity asymmetry in charmless B meson decays is a key issue to be understood. Many theoretical calculations have been performed using short distance factorization approaches which, in general, ...do not take into account the CPT invariance constraint. For each channel with CP violation there is an equal amount of CP asymmetry in another channel or other channels, with an opposite sign. This happens if these channels are coupled through final state interactions. In the specific process B→PV, involving one pseudoscalar and one vector particle in the final state, we argue that the CP asymmetry, inherent from a short distance mechanism, could be suppressed due to the CPT constraint. In this case, we propose a sensitive and practical experimental method to identify even a small CP asymmetry, which provides the values for ACP without the need for an amplitude analysis. This method, if applied directly to data, will enable one to extract the CP asymmetry information in a model independent way and check to which extent the suggested suppression due to the CPT constraint is verified.
3‐Quinoline‐ and 5‐isoquinolineboronic acids (3QBA and 5IQBA) were combined with 1‐naphthyl and diphenyl phosphate (1NP and DPP) to give three crystalline molecular salts of 1:1 stoichiometric ...composition, viz., 3QBA‐1NP, 5IQBA‐1NP and 3QBA‐DPP. The products were characterized by elemental analysis, single‐crystal X‐ray diffraction studies, IR spectroscopy, luminescence spectroscopy and TG‐DSC measurements. The analysis of the solid‐state structures revealed that the molecular components are linked by strong charge‐assisted hydrogen bonds forming heterodimeric synthons of the type B(OH)2···–O2P and N+–H···O(P). In addition, the hydrogen phosphate salts derived from 1NP exhibit the homodimeric P(O)OH···O(HO)P synthon. The aggregates formed through the above‐mentioned hydrogen bonding motifs are further connected through π···π interactions, which involve mainly the aromatic rings of the quinolinium/isoquinolinium cations. The crystal structure analyses were accomplished by Hirshfeld surface analyses and the associated 2D fingerprint plots, illustrating the similarities and differences of the three salts more concisely. Solid‐state photoluminescence spectroscopy of the title compounds showed emission in the blue region with an extraordinary high quantum yield (Φf = 98 %) for compound 3QBA‐DPP.
A series of three new crystalline molecular salts was prepared from quinoline boronic acids and organic phosphates. The supramolecular networks in the solid state and their luminescence behavior were examined.
Wireless Sensor Networks (WSNs) are spearheading the efforts taken to build and deploy systems aiming to accomplish the ultimate objectives of the Internet of Things. Due to the sensors WSNs nodes ...are provided with, and to their ubiquity and pervasive capabilities, these networks become extremely suitable for many applications that so-called conventional cabled or wireless networks are unable to handle. One of these still underdeveloped applications is monitoring physical parameters on a person. This is an especially interesting application regarding their age or activity, for any detected hazardous parameter can be notified not only to the monitored person as a warning, but also to any third party that may be helpful under critical circumstances, such as relatives or healthcare centers. We propose a system built to monitor a sportsman/woman during a workout session or performing a sport-related indoor activity. Sensors have been deployed by means of several nodes acting as the nodes of a WSN, along with a semantic middleware development used for hardware complexity abstraction purposes. The data extracted from the environment, combined with the information obtained from the user, will compose the basis of the services that can be obtained.
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