AIM： TO determine p15 promoter hypermethylation in gastric tumoral tissue and serum samples, its impact on p16-protein expression, and correlation with clinical and histological features. METHODS： ...Samples were obtained from 52 histologically confirmed cases of gastric adenocarcinoma. Gastric tissue and serum of 50 age- and sex-matched individuals with normal gastroscopy and biopsy were obtained as control samples. Methylation-specific polymerase chain reaction （MSP） was used to evaluate methylation status of p16 promoter, p16-protein expression was analyzed by immunohistochemical staining on paraffin-embedded sections. RESULTS： Methylation was detected in 44.2% （23/52） of tumoral tissues. 60.9% of them were also methylated in serum, i.e., 26.9% of all patients （14/52）. Methylation was not detected in tissue and sera of control samples. p16-protein expression was decreased in 61.5% of cases （32/52）, and was significantly associated with promoter hypermethylation （P 〈 0.001）. Methylation was significantly more frequent in higher pathological grades （P 〈 0.05）. Methylation was not associated with other clinicopathological features and environmental factors including Hpylori infection and smoking. CONCLUSION： p16 promoter hypermethylation is an important event in gastric carcinogenesis. It is the principle mechanism of p16 gene silencing. It is related to malignant tumor behavior. Detection of DNA methylation in serum may be a biomarker for early detection of gastric cancer.
To evaluate the relation of cluster of differentiation 44 (CD44) expression with clinicopathological features of gastric adenocarcinoma, and also its effect on prognosis with an emphasis on the ...differences between intestinal and diffuse types.
From 2000 to 2006, 100 patients with gastric adenocarcinoma, who had undergone total or subtotal gastrectomy without any prior treatment, were studied. Haematoxylin and eosin (HE) staining was used for histological evaluation, including the type (Lauren's classification) and grading of the tumor. The expression of CD44 in the gastric adenocarcinoma mucosa and the adjacent mucosa were determined by immunohistochemistry. The survival analysis was obtained using the Kaplan-Meier test.
Of 100 patients, 74 (74%) patients were male. The tumors were categorized as intestinal type (78%) or diffuse type (22%). Sixty-five percent of patients were CD44-positive. CD44 expression was not detected in normal gastric mucosa. Rather, CD44 was more commonly expressed in the intestinal subtype (P=0.002). A significant relation was seen between the grade of tumor and the expression of CD44 (P=0.014). The survival analysis showed a poor prognosis of patients with CD44-positive tumors (P=0.008); and this was more prominent in the intestinal (P=0.001) rather than diffuse type.
Cell adhesion molecule CD44 is highly expressed in gastric adenocarcinoma. CD44 expression is correlated with a poor prognosis in patients with the intestinal type of gastric adenocarcinoma. CD44 can, therefore, be utilized as a prognostic marker for this group of patients.
Fas (Apo-1/CD95) and its specific ligand (FasL) are key elements in apoptosis. They have been studied in different malignancies but there are few published studies about the soluble forms of these ...markers (i.e. sFas/sFasL) in gastric cancer. We have compared the serum levels of sFas/sFasL in gastric adenocarcinoma patients and cases with pre-neoplastic lesions as potential markers for early diagnosis, and investigated their relation with clinicopathological characteristics.
Fifty-nine newly-diagnosed cases of gastric adenocarcinoma who had undergone gastrectomy, along with 62 endoscopically- and histologically-confirmed non-cancer individuals were enrolled in this study. sFas/sFasL serum levels were detected by Enzyme Linked Immunosurbent Assay.
Mean serum sFas level was significantly higher in gastric cancer patients than in control group (305.97 +/- 63.71 (pg/ml) vs. 92.98 +/- 4.95 (pg/ml), P < 0.001); while the mean serum level of sFasL was lower in patients with gastric adenocarcinoma (0.138 +/- 0.04 (pg/ml) vs. 0.150 +/- 0.02 (pg/ml), P < 0.001). Mean serum levels of sFas/sFasL were significantly different in both intestinal/diffuse and cardiac/non-cardiac subtypes when compared to the control group (P < 0.001). There was an increase in the serum level of sFas from the first steps of pre-neoplastic lesions to gastric adenocarcinoma (P < 0.001). Patients who had no lymph node involvement (N0) showed significantly higher serum levels of sFas compared to others (P = 0.044).
Production of sFas may play a critical role in the carcinogenesis of intestinal-type gastric cancer. sFas serum level may serve as a non-invasive tool for early diagnosis of gastric cancer.
A 40-year-old male patient with suicidal ingestion of one tablet of aluminium phosphide was referred to the department of toxicology emergency of Baharloo Hospital, Tehran, Iran. The garlic odor was ...smelled from the patient and abdominal pain and continuous vomiting as well as agitation and heartburn were the first signs and symptoms. Systolic and diastolic blood pressures at the arrival time were 95 and 67 mmHg, respectively. Gastric lavage with potassium permanganate (1:10,000), and 2 vials of sodium bicarbonate through a nasogastric tube was started for the patient and the management was continued with free intravenous infusion of 1 liter of NaCl 0.9% serum plus NaHCO3, hydrocortisone acetate (200 mg), calcium gluconate (1 g) and magnesium sulfate (1 g). Regarding the large intravenous fluid therapy and vasoconstrictor administering (norepinephrine started by 5 µg/min and continued till 15 µg/min), there were no signs of response and the systolic blood pressure was 49 mmHg. At this time, hydroxyethyl starch (HES) (6% hetastarch 600/0.75 in 0.9% sodium chloride) with a dose of 600 cc in 6 hours was started for the patient. At the end of therapy with HES, the patient was stable with systolic and diastolic blood pressure of 110 and 77 mmHg, respectively. He was discharged on the 6th day after the psychological consultation, with normal clinical and paraclinical examinations. This is the first report of using HES in the management of AlP poisoning and its benefit to survive the patient.
Nowadays, the optimal use of energy, speed increase, fuel consumption reduction and environmental pollution prevention are essential in the ship design and manufacturing discussion. So it is critical ...to be able to estimate ship's response to waves, since the resulting added resistance and increasing fuel consumption also increasing environmental pollution. In this study, the numerical solution of the Iran-Bushehr container ship model was determined to simulate the free surface flow around the hull and estimate the total resistance and investigate ship motions in 2 degree-of-freedom (heave and pitch) in regular head waves. Finally, the results of simulation in different conditions are presented and compared. KCS container ship used for validation. CFD codes in Star-CCM+ used to calculate three dimensional, incompressible, unsteady RANS equations.
Every year, about 300,000 people die because of pesticide poisoning worldwide. The most common pesticide agents are organophosphates and phosphides, aluminium phosphide (AlP) in particular. AlP is ...known as a suicide poison that can easily be bought and has no effective antidote. Its toxicity results from the release of phosphine gas as the tablet gets into contact with moisture. Phosphine gas primarily affects the heart, lungs, gastrointestinal tract, and kidneys. Poisoning signs and symptoms include nausea, vomiting, restlessness, abdominal pain, palpitation, refractory shock, cardiac arrhythmias, pulmonary oedema, dyspnoea, cyanosis, and sensory alterations. Diagnosis is based on clinical suspicion, positive silver nitrate paper test to phosphine, and gastric aspirate and viscera biochemistry. Treatment includes early gastric lavage with potassium permanganate or a combination with coconut oil and sodium bicarbonate, administration of charcoal, and palliative care. Specific therapy includes intravenous magnesium sulphate and oral coconut oil. Moreover, acidosis can be treated with early intravenous administration of sodium bicarbonate, cardiogenic shock with fluid, vasopresor, and refractory cardiogenic shock with intra-aortic baloon pump or digoxin. Trimetazidine may also have a useful role in the treatment, because it can stop ventricular ectopic beats and bigeminy and preserve oxidative metabolism. This article reviews the epidemiological, toxicological, and clinical/pathological aspects of AlP poisoning and its management.
•A novel fixed hybrid mono-pile WT combined with pitching types WECs was proposed.•Hydrodynamic modeling of the study area was performed to find suitable areas for energy harvesting.•The optimum ...damping coefficient between WECs and the fixed WT was found.•The range of motion response of wave energy absorbers was investigated.•By adding the WECs to WT, the hybrid system can produce 26.44% more power.
Due to the high cost of using offshore wind turbines, increasing the efficiency and amount of energy absorbed in different ways needs to be considered. This research work aims to study the dynamics and power absorption of the wave energy converter by proposing a new hybrid mono-pile wind turbine with two pitching WECs. Each WEC has a power-take-off mechanism activated by the pitch motion of the device linked to the mono-pile via a hinged type connection. The installation point was off the coast of Dayyer port in the Persian Gulf. First, the study area's wave, current, and wind characteristics were modeled and analyzed. Then a numerical model was developed to investigate and analyze the dynamics of the hybrid system and the effect of the damping coefficient between WT and WECs concerning power production under operational conditions. Hydrodynamic forces exerted on each part of the system were estimated using BEM in the time domain. The connections were also modeled as a linear, rotational damper and stiffness. The WECs' PTO's damping coefficient was established to optimize wave energy generation. By analyzing the damping coefficient of connections between the wind turbine and WECs, appropriate coefficients were obtained for maximum WECs performance. The results show that for this fixed hybrid arrangement installed in the coastal zone of Dayyer port in the Persian Gulf, 26% of the wind turbine energy is added to the device's energy by employing wave absorption systems.
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Brain weight of Iranian population; the first report Mehrpour, Omid, M.D; Sheikhazadi, Ardeshir, M.D; Ghadyani, Mohammad Hasan, M.D ...
Journal of forensic and legal medicine,
11/2010, Letnik:
17, Številka:
8
Journal Article
Recenzirano
Abstract Objective Knowledge of the normal range of brain weight is a useful parameter in diagnosis of many pathological conditions. There are racial differences as well as other variables in any ...population affecting the brain weight. We conducted this study to determine the brain weight in healthy adult Iranian population among both sexes in order to formulate a standard reference range taking into account the variables of age, sex, weight, BMI and height. Materials & methods Prospective analysis of data from 1308 forensic autopsies of Iranian adults from 23 June 2008 to 22 June 2009 was performed. All subjects were Iranian adults dying of short survival injury time (<15 min) from unnatural deaths who showed no pathological changes. Results Brain weight, body weight, body height and BMI were evaluated in 1143 subjects, including 856 males and 287 females, ranging 16–88 years old. Brain weight in males and females was 1322.45 ± 117.05 and 1219.03 ± 127.85 gm respectively ( P = 0.001). A negative significant correlation between age and brain weight was seen in both sexes ( P = 0.001). Moreover, there was a positive significant correlation between body height and Brain weight in males and females ( P = 0.001). Conclusions The study indicated higher values in males than females. The observed correlation of brain weight with age, weight and body height was more prominent in females. The results revealed that body height and age in males and body height, weight and age in females may play a predictive role in estimation of brain weight.
Background: Coronary artery disease has recently increased from 20 to 45%. Previous studies emphasize a wide range of risk factors for coronary atherosclerosis. The role of inflammatory factors has ...been proven in the development of coronary artery atherosclerosis. Drugs are effective in coronary artery atherosclerosis by stimulating inflammatory factors. Objectives: This study evaluated the heart status regarding the history of drug and psychotropics use in corpses referred to Birjand Legal Medicine Center from 2011 to 2018. Materiald and Methods: In this case-control study, corpses referred to forensic medicine with a history of substance abuse (case group) and no history of substance abuse (control group) were selected. The data included pathological and toxicological autopsy results. Data analysis was performed using Mann-Whitney and chi-square tests and Spearman correlation coefficient. Results: Of 193 corpses, 126 were in the case group and 67 in the control group. The mean age was 41.82 ± 16.84. The Mann-Whitney test showed that heart weight was significantly higher in cases than in controls (P = 0.018). Mean heart dimensions in the case and control groups were 13 × 10 × 7 and 13 × 9 × 6, respectively, with a significant difference in one dimension (P = 0.014). Coronary artery stenosis results showed mean RC = 52.49, LC = 55.97, CX = 61.84, and LAD = 69.90. There was no significant positive correlation between drug abuse and atherosclerosis (r = 0.119, P = 0.09). Also, a higher prevalence of atherosclerosis was seen in males. Conclusions: Drug use can be a risk factor for cardiovascular diseases in people with substance abuse by changing the physical characteristics of the heart. Further studies must investigate the effect of drug use on coronary artery disease development and the factors intensifying these effects.
Berberis vulgaris fruit (barberry) is known for its antiarrhythmic and sedative effects in Iranian traditional medicine. The effects of crude aqueous extract of barberry on rat arterial blood ...pressure and the contractile responses of isolated rat aortic rings and mesenteric bed to phenylephrine were investigated. We also examined effect of the extract on potassium currents recorded from cells in parabrachial nucleus and cerebellum rejoins of rat brain. Administration of the extract (0.05–1
mg/100
g body weight of rat) significantly reduced the mean arterial blood pressure and heart rate in anaesthetized normotensive and desoxycorticosteron acetate-induced hypertensive rats in a dose-dependent manner. Concentration–response curves for phenylephrine effects on isolated rat aortic rings and the isolated mesenteric beds in the presence of the extract were significantly shifted to the right. Application of the extract (1–50
μg/ml) shifted the activation threshold voltage to more negative potentials, leading to an enhancement in magnitude of the outward potassium current recorded from cells present in rat brain slices of parabrachial nucleus and cerebellum. This effect on potassium current may explain the sedative and neuroprotective effects of barberry. The present data support the hypothesis that the aqueous extract of barberry has beneficial effects on both cardiovascular and neural system suggesting a potential use for treatment of hypertension, tachycardia and some neuronal disorders, such as epilepsy and convulsion.
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