The potential of sponge-derived chemicals for pharmaceutical applications remains largely unexploited due to limited available biomass. Although many have attempted to culture marine sponge cells in ...vitro to create a scalable production platform for such biopharmaceuticals, these efforts have been mostly unsuccessful. We recently showed that Geodia barretti sponge cells could divide rapidly in M1 medium. In this study we established the first continuous marine sponge cell line, originating from G. barretti. G. barretti cells cultured in OpM1 medium, a modification of M1, grew more rapidly and to a higher density than in M1. Cells in OpM1 reached 1.74 population doublings after 30 min, more than twofold higher than the already rapid growth rate of 0.74 population doublings in 30 min in M1. The maximum number of population doublings increased from 5 doublings in M1 to at least 98 doublings in OpM1. Subcultured cells could be cryopreserved and used to inoculate new cultures. With these results, we have overcome a major obstacle that has blocked the path to producing biopharmaceuticals with sponge cells at industrial scale for decades.
Probing Cold Dense Nuclear Matter Subedi, R; Shneor, R; Monaghan, P ...
Science (American Association for the Advancement of Science),
06/2008, Letnik:
320, Številka:
5882
Journal Article
Recenzirano
The protons and neutrons in a nucleus can form strongly correlated nucleon pairs. Scattering experiments, in which a proton is knocked out of the nucleus with high-momentum transfer and high missing ...momentum, show that in carbon-12 the neutron-proton pairs are nearly 20 times as prevalent as proton-proton pairs and, by inference, neutron-neutron pairs. This difference between the types of pairs is due to the nature of the strong force and has implications for understanding cold dense nuclear systems such as neutron stars.
Archaeological bone undergoes alterations after burial (diagenesis) that constitute a problem for the survival of archaeological information. A common method to assess this alteration is Fourier ...transform infrared spectrometry (FTIR). However, the commonly applied method (FTIR–KBr) is destructive and sample preparation may influence the results. This paper tests the suitability of FTIR attenuated total reflection (FTIR–ATR), a method not commonly used to investigate bone diagenesis. FTIR–ATR requires less sample preparation and can be non‐destructive, allowing analysis of bone cross‐sections. Modern and archaeological bones were analysed using both methods and different sample preparation methods were tested. The results show that FTIR–ATR has advantages for the rapid assessment of bone diagenesis.
A new reflected shock tunnel capable of generating hypersonic environments at realistic flight enthalpies has been commissioned at Sandia. The tunnel uses an existing free-piston driver and shock ...tube coupled to a conical nozzle to accelerate the flow to approximately Mach 9. The facility design process is outlined and compared to other ground test facilities. A representative flight-enthalpy condition is designed using an in-house state-to-state solver and piston dynamics model and evaluated using quasi-1D modeling with the University of Queensland L1d code. This condition is demonstrated using canonical models and a calibration rake. A 25-cm core flow with 4.6-MJ/kg total enthalpy is achieved over an approximately 1-ms test time. The condition was refined using analysis and a heavier piston, leading to an increase in test time. A novel high-speed molecular tagging velocimetry method is applied using in situ nitric oxide to measure the freestream velocity of approximately 3016 m/s. Companion simulation data show good agreement in exit velocity, pitot pressure, and core flow size.
A line list for the N2 second positive system, B3Πg - C3Πu, has been compiled using the PGOPHER spectral simulation software. The line list extends the number of vibrational states of the B3Πg up to ...v= 29 and a maximum rotational state of J= 150 for simulation temperatures up to 7000 K. New electronic–vibrational transition moments were calculated using refined potential energy curves and a transition dipole moment with the DUO software. Comparisons to experimental data and the SPECAIR software have been used to validate the new line list. The results are available in ASCII ExoMol .state and .trans files and as a PGOPHER input file for use in spectral analysis.
•New validated N2(C3Πu−B3Πg) second positive system linelist containing 1,207,500 transitions between 31,500 states.•New electronic–vibrational transition moments were calculated for the band system.•Comparison with SPECAIR and high-resolution experimental emission data.
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