Memory formation is a complex cognitive function regulated by coordinated synaptic and nuclear processes in neurons. In mammals, it is controlled by multiple molecular activators and suppressors, ...including the key signalling regulator, protein phosphatase 1 (PP1). Here, we show that memory control by PP1 involves the miR-183/96/182 cluster and its selective regulation during memory formation. Inhibiting nuclear PP1 in the mouse brain, or training on an object recognition task similarly increases miR-183/96/182 expression in the hippocampus. Mimicking this increase by miR-183/96/182 overexpression enhances object memory, while knocking-down endogenous miR-183/96/182 impairs it. This effect involves the modulation of several plasticity-related genes, with HDAC9 identified as an important functional target. Further, PP1 controls miR-183/96/182 in a transcription-independent manner through the processing of their precursors. These findings provide novel evidence for a role of miRNAs in memory formation and suggest the implication of PP1 in miRNAs processing in the adult brain.
The understanding of the nonlinear optical (NLO) properties of photonic materials is relevant and necessary for basic studies and technological developments. Among the materials with high optical ...nonlinearities, layered transition-metal dichalcogenides (LTMDs) have attracted considerable attention from the viewpoint of both synthesis as well as characterization and applications. We report here the third-order NLO properties of one LTMDthe metallic NbS2that was prepared by a modified redox exfoliated method, suspended in acetonitrile. The LTMD was morphologically and compositionally characterized using transmission electron microscopy, atomic force microscopy, X-ray diffraction, Raman scattering, and linear absorption. The Z-scan technique was employed to characterize the nonlinear refraction (NLR) and nonlinear absorption (NLA) behavior in the femtosecond regime. Metallic NbS2 presented a sign inversion of the nonlinear refractive index from negative (self-defocusing) to positive (self-focusing) with the increase of intensity up to 100 GW/cm2. Two distinct features characteristic of NLA, two-photon absorption and saturated absorption, were also observed for different intensity ranges, with the former evolving into the latter as the intensity increased in the same range as for the NLR measurements. The material’s band structure of NbS2 was calculated using the density functional theory, and the origin of the nonlinearities is discussed by comparison with the experimental data.
doi: https://doi.org/10.12669/pjms.36.5.3009
How to cite this:Jawaid SA, Jawaid M. Challenges faced by the Medical Editors in COVID19 Pandemic era. Pak J Med Sci. 2020;36(5):855-856. doi: ...https://doi.org/10.12669/pjms.36.5.3009
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Microtubules are highly dynamic structures that form spindle fibres during mitosis and are one of the most validated cancer targets. The success of drugs targeting microtubules, however, is often ...limited by the development of multidrug resistance. Here we describe the discovery and characterization of SSE15206, a pyrazolinethioamide derivative 3-phenyl-5-(3,4,5-trimethoxyphenyl)-4,5-dihydro-1H-pyrazole-1-carbothioamide that has potent antiproliferative activities in cancer cell lines of different origins and overcomes resistance to microtubule-targeting agents. Treatment of cells with SSE15206 causes aberrant mitosis resulting in G2/M arrest due to incomplete spindle formation, a phenotype often associated with drugs that interfere with microtubule dynamics. SSE15206 inhibits microtubule polymerization both in biochemical and cellular assays by binding to colchicine site in tubulin as shown by docking and competition studies. Prolonged treatment of cells with the compound results in apoptotic cell death increased Poly (ADP-ribose) polymerase cleavage and Annexin V/PI staining accompanied by p53 induction. More importantly, we demonstrate that SSE15206 is able to overcome resistance to chemotherapeutic drugs in different cancer cell lines including multidrug-resistant KB-V1 and A2780-Pac-Res cell lines overexpressing MDR-1, making it a promising hit for the lead optimization studies to target multidrug resistance.
How to cite this:Jawaid SA, Jawaid M. Common reasons for not accepting manuscripts for further processing after editor’s triage and initial screening. Pak J Med Sci. 2019;35(1):1-3. doi: ...https://doi.org/10.12669/pjms.35.1.28
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
doi: https://doi.org/10.12669/pjms.36.6.3154
How to cite this:Jawaid SA, Jawaid M. Some useful tips to detect Gift Authorship. Pak J Med Sci. 2020;36(6):1145-1146. doi: ...https://doi.org/10.12669/pjms.36.6.3154
doi: https://doi.org/10.12669/pjms.39.2.7398
How to cite this: Shah FA, Jawaid SA. The h-Index: An Indicator of Research and Publication Output. Pak J Med Sci. 2023;39(2):315-316. doi: ...https://doi.org/10.12669/pjms.39.2.7398
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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